FastQCFastQC Report
Wed 25 May 2016
SRR1780232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177795
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT77424.354453162349897No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC23301.310498045501842No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG20901.1755111223600214No Hit
CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG19751.1108298883545655TruSeq Adapter, Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC5080.285722320650187No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2630.14792316994291177TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT2040.11473888467054753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA650.059.2307662
TTTATAC900.058.333333
ACAAAAG200.006942420752.557
CTGTAGA200.006942420752.51
CTATAAC200.006942420752.52
CTTGCAT200.006942420752.513
TGTATAA305.865926E-446.66666411
GGTTTTC350.001252907740.037
CATTACC350.001252907740.029
CCAGTAG350.001252907740.03
CATACAG459.170543E-538.8888855
CCCGACA459.170543E-538.88888521
CTTGAAA16300.035.85889457
TTGAAAA16350.035.74923358
TATGCCG16750.035.73134643
CGTATGC16850.035.51928741
CTCGTAT16850.035.51928739
TCGTATG16850.035.51928740
TGAAAAA16550.035.3172259
GTATGCC17000.035.20588342