##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780232_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 177795 Sequences flagged as poor quality 0 Sequence length 76 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.73862594561152 34.0 34.0 34.0 34.0 34.0 2 33.73351894035265 34.0 34.0 34.0 34.0 34.0 3 33.753080795297954 34.0 34.0 34.0 34.0 34.0 4 33.77342444950646 34.0 34.0 34.0 34.0 34.0 5 33.77229955848027 34.0 34.0 34.0 34.0 34.0 6 37.52878877358756 38.0 38.0 38.0 37.0 38.0 7 37.54524030484547 38.0 38.0 38.0 37.0 38.0 8 37.454394105571026 38.0 38.0 38.0 37.0 38.0 9 37.46483871874912 38.0 38.0 38.0 37.0 38.0 10-11 37.41997244016986 38.0 38.0 38.0 37.0 38.0 12-13 37.41150482297027 38.0 38.0 38.0 37.0 38.0 14-15 37.34463848814646 38.0 38.0 38.0 37.0 38.0 16-17 37.37592733203971 38.0 38.0 38.0 36.5 38.0 18-19 37.335121910064956 38.0 38.0 38.0 36.5 38.0 20-21 37.351449703309996 38.0 38.0 38.0 36.5 38.0 22-23 37.32156978542704 38.0 38.0 38.0 36.0 38.0 24-25 37.33795663545094 38.0 38.0 38.0 36.5 38.0 26-27 37.259076464467505 38.0 38.0 38.0 36.0 38.0 28-29 37.23347956916673 38.0 38.0 38.0 36.0 38.0 30-31 37.192553221406676 38.0 38.0 38.0 36.0 38.0 32-33 37.16455187153744 38.0 38.0 38.0 36.0 38.0 34-35 37.18945133440198 38.0 38.0 38.0 36.0 38.0 36-37 37.16959138333474 38.0 38.0 38.0 36.0 38.0 38-39 36.79408588542985 38.0 38.0 38.0 35.0 38.0 40-41 36.98952164009111 38.0 38.0 38.0 35.5 38.0 42-43 37.113501504541745 38.0 38.0 38.0 36.0 38.0 44-45 37.16699288506426 38.0 38.0 38.0 36.0 38.0 46-47 37.10913411513259 38.0 38.0 38.0 36.0 38.0 48-49 37.151328777524675 38.0 38.0 38.0 36.0 38.0 50-51 37.02651649371467 38.0 38.0 38.0 36.0 38.0 52-53 37.01756236114626 38.0 38.0 38.0 36.0 38.0 54-55 37.00152985179561 38.0 38.0 38.0 36.0 38.0 56-57 37.04400854917179 38.0 38.0 38.0 36.0 38.0 58-59 36.95816249050873 38.0 38.0 38.0 35.5 38.0 60-61 36.96222334711325 38.0 38.0 38.0 36.0 38.0 62-63 36.93927275795157 38.0 38.0 38.0 35.5 38.0 64-65 36.891667369723564 38.0 38.0 38.0 35.5 38.0 66-67 36.65199527545769 38.0 37.5 38.0 34.5 38.0 68-69 36.599460052307435 38.0 38.0 38.0 34.0 38.0 70-71 36.12618183863438 38.0 37.0 38.0 32.5 38.0 72-73 35.36972918248544 38.0 37.0 38.0 27.0 38.0 74-75 35.121083270058215 38.0 37.0 38.0 25.0 38.0 76 34.32309120053995 38.0 35.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10314806739097548 1101 2 0.0786462074218619 1101 3 0.1637391044154981 1101 4 0.04574116078661916 1101 5 0.0062498496535852155 1101 6 -0.13566717379977433 1101 7 0.004139231435949853 1101 8 0.006932556844418514 1101 9 0.10360406390541499 1101 10-11 0.07885838566498649 1101 12-13 0.11087902165575514 1101 14-15 -0.014007535213160338 1101 16-17 0.15548263933406048 1101 18-19 0.06278659917925467 1101 20-21 0.10527810703406004 1101 22-23 0.06914798996900373 1101 24-25 0.04263916079784735 1101 26-27 0.004784311826291798 1101 28-29 -0.38662989503006173 1101 30-31 -0.17530187031309907 1101 32-33 0.03536250747794867 1101 34-35 0.04967108946480181 1101 36-37 0.04064138388454097 1101 38-39 0.22017884136442234 1101 40-41 0.27862207770622405 1101 42-43 0.27831346024318293 1101 44-45 0.3168972973348758 1101 46-47 0.16522679872904433 1101 48-49 0.20408890895946996 1101 50-51 0.21881378012669472 1101 52-53 0.2950834878353774 1101 54-55 0.029985809408493935 1101 56-57 -0.19293870534303892 1101 58-59 0.16884044726108272 1101 60-61 0.028485628965846388 1101 62-63 0.16147384678095733 1101 64-65 0.027140787381796372 1101 66-67 -0.006010773484604215 1101 68-69 -0.4230308706276773 1101 70-71 0.023110450527660475 1101 72-73 -0.142122115746794 1101 74-75 -0.020266189243287158 1101 76 -0.8462325280675813 1102 1 -0.12048605710521798 1102 2 -0.10402098171083907 1102 3 -0.17004205695187125 1102 4 0.10127183700694786 1102 5 -0.0747790352546076 1102 6 0.026176011750308703 1102 7 0.04545136757567292 1102 8 -0.049503105194688146 1102 9 0.006537337528342846 1102 10-11 0.035600996502431315 1102 12-13 -0.07307567307480412 1102 14-15 0.11307299919496216 1102 16-17 0.1329233021195506 1102 18-19 0.10234940706103401 1102 20-21 0.0596416314406909 1102 22-23 0.16930752870613475 1102 24-25 -0.13642207968580067 1102 26-27 0.021032072686132608 1102 28-29 -0.021566079535212168 1102 30-31 -0.16230552757537708 1102 32-33 -0.10341286944397865 1102 34-35 -0.1051981074302617 1102 36-37 0.10319736238879074 1102 38-39 0.06672310170178974 1102 40-41 0.21044492439975926 1102 42-43 0.043054466363493304 1102 44-45 0.03544204281926966 1102 46-47 0.10142529850513426 1102 48-49 0.10380808346312875 1102 50-51 0.10839151560959692 1102 52-53 0.18278127855041504 1102 54-55 0.052885682523886146 1102 56-57 0.17449486683191395 1102 58-59 0.19321908586385206 1102 60-61 0.150602735996749 1102 62-63 0.13373649792291786 1102 64-65 0.2885930563771737 1102 66-67 -0.01409500247400075 1102 68-69 -0.05334416365648309 1102 70-71 -0.13440839556877648 1102 72-73 -0.07860983779486475 1102 74-75 0.1932684801103477 1102 76 0.28124918562102863 1103 1 -0.08820951012278755 1103 2 -0.004644867628847749 1103 3 -0.05634203148713368 1103 4 0.024877642965691393 1103 5 0.047451675211391375 1103 6 -0.04665311990235921 1103 7 0.1333046907231079 1103 8 -0.021555562541266227 1103 9 -0.026503151394500435 1103 10-11 0.00934049230075118 1103 12-13 -0.028989856930159874 1103 14-15 0.028498260208458248 1103 16-17 -0.03141970781169334 1103 18-19 -0.03945731562804866 1103 20-21 -0.042822081317140714 1103 22-23 0.014227314802390367 1103 24-25 -0.047995800083057816 1103 26-27 -0.02680979133220518 1103 28-29 0.029427044987322404 1103 30-31 -0.004137714995799513 1103 32-33 0.005289702493882942 1103 34-35 -0.059998209289183535 1103 36-37 0.13012631578833833 1103 38-39 0.06341268662666977 1103 40-41 0.10002348309596698 1103 42-43 -0.06278332329781477 1103 44-45 -9.725230070571911E-4 1103 46-47 -0.026248498286307154 1103 48-49 -0.059611829956786266 1103 50-51 -0.1234330323914179 1103 52-53 0.031011479721790636 1103 54-55 0.18339243067769218 1103 56-57 -0.10580816347112432 1103 58-59 0.1287614846415508 1103 60-61 0.20388768634052212 1103 62-63 0.1034652985340756 1103 64-65 0.19364143936672917 1103 66-67 0.057874694495687606 1103 68-69 0.1163146089434619 1103 70-71 0.15786305846738458 1103 72-73 0.4964156269364821 1103 74-75 0.35595628250403877 1103 76 0.831542030031521 1104 1 -0.06787764856460399 1104 2 -0.12777547580432014 1104 3 -0.027304145146160863 1104 4 -0.07421792746586675 1104 5 -0.021840448417719927 1104 6 0.0654213709109186 1104 7 -0.06226930158567967 1104 8 -0.17041256296855778 1104 9 -0.12228722773690492 1104 10-11 -0.04521014151210068 1104 12-13 0.04191350441437436 1104 14-15 0.058355118205902556 1104 16-17 0.09444377317392139 1104 18-19 -0.02537456388328252 1104 20-21 -0.04019603089697199 1104 22-23 0.010684372201616554 1104 24-25 -0.05396005018987182 1104 26-27 0.12972951930730403 1104 28-29 -0.09772720654380151 1104 30-31 0.04076329625615216 1104 32-33 -0.18930508457348338 1104 34-35 0.18356574171495055 1104 36-37 0.10398153584245762 1104 38-39 0.0868869068232172 1104 40-41 0.0667394067535696 1104 42-43 0.035217317636522694 1104 44-45 -0.09596433329049603 1104 46-47 -0.1527707173434223 1104 48-49 -0.006040401385355665 1104 50-51 -0.17936345540807963 1104 52-53 -0.18577565886246106 1104 54-55 -0.1200543717721061 1104 56-57 -0.2713650641975107 1104 58-59 0.037374930019701935 1104 60-61 -0.033320332460220925 1104 62-63 -0.11975788904604201 1104 64-65 -0.015579113261097177 1104 66-67 -0.4383420125260997 1104 68-69 -0.34290594368436444 1104 70-71 -0.28495135543376904 1104 72-73 0.02658405023800725 1104 74-75 -0.2599104031532775 1104 76 0.1207458471515892 1105 1 -0.06932793687493444 1105 2 5.400929533223575E-4 1105 3 0.1001770709992158 1105 4 0.04083536626119866 1105 5 0.12701504695183985 1105 6 0.006392015677647578 1105 7 0.03835537078894902 1105 8 -0.0316872440798619 1105 9 -0.005940905877736213 1105 10-11 0.04966579768063184 1105 12-13 0.16626917451746692 1105 14-15 0.12007973816046302 1105 16-17 0.01359675238062863 1105 18-19 0.06140684446898348 1105 20-21 -0.009397061824806485 1105 22-23 0.003944026229227404 1105 24-25 -0.018441091601722803 1105 26-27 0.06804639856643036 1105 28-29 0.10424728236062464 1105 30-31 -0.05580755819080707 1105 32-33 0.00948488572181816 1105 34-35 -0.05286401540544006 1105 36-37 0.003096947339919609 1105 38-39 0.07154748693854174 1105 40-41 0.06568882139623611 1105 42-43 -0.12479709787981363 1105 44-45 -0.12054715698435814 1105 46-47 -0.19250293820660858 1105 48-49 9.183615012418045E-4 1105 50-51 -0.1514781679714261 1105 52-53 0.03740929658049197 1105 54-55 -0.223286942102348 1105 56-57 -0.05978459940631353 1105 58-59 0.07964297125680986 1105 60-61 0.1302586470959568 1105 62-63 -0.035322278352353464 1105 64-65 -0.189982835085317 1105 66-67 -0.36948171198442736 1105 68-69 0.06851507675945356 1105 70-71 -0.10446909088719991 1105 72-73 0.10097466704737457 1105 74-75 0.09910147872685116 1105 76 -0.360487771579848 1106 1 0.08979131983882382 1106 2 0.06489035386716324 1106 3 0.09229026093600368 1106 4 0.08235248475426005 1106 5 0.12104160501047545 1106 6 0.10201375425471326 1106 7 0.08037734694609355 1106 8 0.10941410927807738 1106 9 0.18033884466318284 1106 10-11 0.04250869785457212 1106 12-13 0.04062749618392303 1106 14-15 0.08717913996777327 1106 16-17 0.0014631984372215356 1106 18-19 0.06266686737848914 1106 20-21 0.1761105534651719 1106 22-23 0.08558672276310375 1106 24-25 0.16504497178124922 1106 26-27 0.11163385773235746 1106 28-29 0.05832008368428632 1106 30-31 0.18468287496857982 1106 32-33 0.14185729249284407 1106 34-35 0.16807697140037448 1106 36-37 0.043914053105488904 1106 38-39 0.20652054705478662 1106 40-41 0.15305889479150636 1106 42-43 0.12416985303444505 1106 44-45 0.1715384566934688 1106 46-47 0.2295050376959864 1106 48-49 0.17863038050764857 1106 50-51 0.22944330332805407 1106 52-53 0.22660576681933975 1106 54-55 0.3255060662553788 1106 56-57 0.25706661607033254 1106 58-59 0.18037904524604187 1106 60-61 0.002561476844377353 1106 62-63 0.1387602807244832 1106 64-65 0.006152246158578123 1106 66-67 -0.021269926315596877 1106 68-69 0.10938097129086799 1106 70-71 0.3173713062109229 1106 72-73 0.5517680148052122 1106 74-75 0.33017992257734363 1106 76 0.5655739143901997 1107 1 -0.14105769857849992 1107 2 -0.04490523407050517 1107 3 0.07251524913002783 1107 4 0.07881069159498111 1107 5 -0.03222527753691651 1107 6 0.03798319363945524 1107 7 0.13316693070106567 1107 8 0.06611147148546337 1107 9 0.08134572586131839 1107 10-11 0.042626757626550216 1107 12-13 -0.013414364438084192 1107 14-15 0.20006114765852345 1107 16-17 0.1361567490005342 1107 18-19 0.061073060456813266 1107 20-21 0.11451708385713033 1107 22-23 0.06958962366035593 1107 24-25 0.20496604039084332 1107 26-27 0.1644613892711817 1107 28-29 0.1668254469710675 1107 30-31 0.01926426874852183 1107 32-33 0.16931883873854758 1107 34-35 -0.2575116408913303 1107 36-37 -9.781751883863876E-4 1107 38-39 -0.12662726377972433 1107 40-41 0.10541334395834667 1107 42-43 0.16310006284351886 1107 44-45 0.167077483880945 1107 46-47 0.19128318651875986 1107 48-49 0.06568091009005883 1107 50-51 -0.015446395113556832 1107 52-53 0.29632006217116214 1107 54-55 0.29134462795004623 1107 56-57 -0.034233222019125265 1107 58-59 0.012784766085275123 1107 60-61 -0.10372183287699954 1107 62-63 0.08929323626654195 1107 64-65 0.09887976420486666 1107 66-67 0.2405444837228572 1107 68-69 0.4406103108307704 1107 70-71 0.07799668568876683 1107 72-73 0.22445151342222402 1107 74-75 0.22904486219820797 1107 76 -0.34983625898900783 1108 1 -0.10743154405070499 1108 2 -0.03292541061527032 1108 3 -0.0030828899927044517 1108 4 -0.13111739538228306 1108 5 -0.16069312116869838 1108 6 -0.15553866996437904 1108 7 -0.10653178440747979 1108 8 0.03213597644439403 1108 9 -0.16628983529883357 1108 10-11 -0.038871827970396566 1108 12-13 -0.14402724402637546 1108 14-15 -0.09830121217924415 1108 16-17 -0.06834164914540253 1108 18-19 0.027208331919958084 1108 20-21 -0.0969787751797142 1108 22-23 -0.0942127348141284 1108 24-25 -0.09681304007676772 1108 26-27 -0.018718717064665213 1108 28-29 0.019595461626330746 1108 30-31 -0.07142793669778769 1108 32-33 -0.18455245058356695 1108 34-35 -0.15891241114456278 1108 36-37 -0.015299006107575508 1108 38-39 -0.15379061881193223 1108 40-41 -0.0774451134902776 1108 42-43 -0.021869348560315416 1108 44-45 -0.0370012796240502 1108 46-47 -0.10598215890232154 1108 48-49 -0.11232938267433923 1108 50-51 -0.2325828253647444 1108 52-53 -0.06923922347009182 1108 54-55 -0.2853526557144548 1108 56-57 -0.0867258943888487 1108 58-59 -0.08528619264142634 1108 60-61 -0.09376775837374396 1108 62-63 -0.25032538613896804 1108 64-65 -0.09078657300246107 1108 66-67 -0.2289904673694707 1108 68-69 -0.3814709917833099 1108 70-71 -0.018310529470909387 1108 72-73 0.16575165656662705 1108 74-75 0.04667633351820655 1108 76 0.7803356847075236 1109 1 -0.056871248677133224 1109 2 -0.10697584151217399 1109 3 -0.04401593922450786 1109 4 -0.010060813744793506 1109 5 -0.05616072410517603 1109 6 -0.303600712217289 1109 7 -0.09290612555361122 1109 8 -0.1493755702123849 1109 9 0.05713818895906542 1109 10-11 -0.04486729039739146 1109 12-13 -0.15595993956902987 1109 14-15 -0.03869812041848064 1109 16-17 -0.10219013216401152 1109 18-19 -0.05651247794192926 1109 20-21 -0.025932545419792064 1109 22-23 -0.26480089399149875 1109 24-25 -0.10458773146141453 1109 26-27 -0.17790902770725125 1109 28-29 -0.27549990504580535 1109 30-31 -0.09654417965635531 1109 32-33 -0.1855337223117175 1109 34-35 -0.26060666844048086 1109 36-37 -0.19713404893847297 1109 38-39 -0.12407641689855353 1109 40-41 -0.17577501057536438 1109 42-43 -0.2544523145457731 1109 44-45 -0.0486816481508896 1109 46-47 -0.06343162879992548 1109 48-49 -0.24411094080443974 1109 50-51 -0.1470870998275231 1109 52-53 -0.1967161258432597 1109 54-55 -0.27855707941680663 1109 56-57 0.03675259527221186 1109 58-59 0.08820480553837484 1109 60-61 -0.010441613844278663 1109 62-63 -0.46231889896235145 1109 64-65 -0.21376903519654178 1109 66-67 -0.0752758686341295 1109 68-69 -0.1650585212214679 1109 70-71 -0.03401950368610329 1109 72-73 -0.4161554180790361 1109 74-75 -0.47125613773376784 1109 76 -0.020649468974724527 1110 1 0.1291606001389738 1110 2 -0.06404213783056178 1110 3 0.04131469033917057 1110 4 0.13807281836645302 1110 5 -0.020430919304850192 1110 6 -0.21138143422603406 1110 7 -0.1878348533902212 1110 8 0.03843701765303109 1110 9 -0.06946468919237248 1110 10-11 -0.12478328041162001 1110 12-13 0.058212272381517494 1110 14-15 -0.19507299580934756 1110 16-17 -0.04039465302592049 1110 18-19 0.1902050832123976 1110 20-21 0.0035668027785931145 1110 22-23 -0.23967671087358866 1110 24-25 0.039769285417264655 1110 26-27 -0.16220614699972913 1110 28-29 0.12859792483823185 1110 30-31 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0.296407589038445 2115 62-63 0.195994415443991 2115 64-65 0.23912278980164103 2115 66-67 0.41517419324255656 2115 68-69 0.44907666113275724 2115 70-71 0.20930600406667565 2115 72-73 0.4036469405145411 2115 74-75 0.38787431287407514 2115 76 0.1528600309160808 2116 1 -0.018414925622323608 2116 2 -0.10431807024322381 2116 3 -0.09228974390543954 2116 4 -0.08467729011864833 2116 5 -0.030979697337627954 2116 6 -0.08789183761166441 2116 7 0.04709242804025848 2116 8 -0.020699664626540937 2116 9 -0.03389499702164045 2116 10-11 -0.1160485522059389 2116 12-13 -0.05789586377734679 2116 14-15 0.021456554637346414 2116 16-17 -0.36479194559569805 2116 18-19 -0.11707169471301171 2116 20-21 -0.1773147672804143 2116 22-23 -0.10746580689072971 2116 24-25 -0.17101167309892418 2116 26-27 0.024972032508436826 2116 28-29 -0.0791163723796231 2116 30-31 -0.018415648391382433 2116 32-33 -0.14990015122538125 2116 34-35 -0.005609787253703757 2116 36-37 -0.15325370288579876 2116 38-39 -0.1594320068062629 2116 40-41 -0.3700589943394519 2116 42-43 -0.21618357228630458 2116 44-45 -0.1832787788427268 2116 46-47 -0.0813761048845052 2116 48-49 -0.012304017943087331 2116 50-51 -0.02146446718756323 2116 52-53 -0.23206041570305302 2116 54-55 -0.11990666673905537 2116 56-57 -0.23784986904223615 2116 58-59 0.02974965768854787 2116 60-61 -0.28132862240519785 2116 62-63 -0.22313756483349323 2116 64-65 -0.0676797557779878 2116 66-67 -0.06447980285880561 2116 68-69 -0.1044292441533301 2116 70-71 -0.03448054564092473 2116 72-73 -0.17620342068256178 2116 74-75 -0.04993385132727468 2116 76 0.13686415888305703 2117 1 -0.006427469952463127 2117 2 -0.08846180247453361 2117 3 0.05194074350627176 2117 4 -0.08453562906957757 2117 5 0.05127652353637302 2117 6 0.07945382511780252 2117 7 -0.032237759440810976 2117 8 -0.2860259246771548 2117 9 0.028260009475225445 2117 10-11 -0.03294480746938433 2117 12-13 0.046117540288811654 2117 14-15 0.2043800479011182 2117 16-17 0.0583935717602202 2117 18-19 0.0751232699694242 2117 20-21 0.1439407404034725 2117 22-23 0.020859564114672935 2117 24-25 -0.28031294218652647 2117 26-27 -0.10943499387959577 2117 28-29 -0.5067300187350341 2117 30-31 0.1599869034614514 2117 32-33 -0.040933495305409906 2117 34-35 0.15853628971220957 2117 36-37 0.15747492339281166 2117 38-39 0.34558474827644403 2117 40-41 0.29660278746345625 2117 42-43 0.034576024028552865 2117 44-45 0.029249431245496282 2117 46-47 0.055839718166190266 2117 48-49 -0.04695968389656713 2117 50-51 0.17702952931487914 2117 52-53 -0.042254149399816754 2117 54-55 -0.03566963360990627 2117 56-57 0.0285608307633467 2117 58-59 -0.09603314172917976 2117 60-61 0.03131731289966666 2117 62-63 0.12373416841386131 2117 64-65 -0.1620553236434077 2117 66-67 -0.016356567515842357 2117 68-69 0.05265820467771931 2117 70-71 0.3976320602385002 2117 72-73 -0.004041282284610759 2117 74-75 0.532148956883205 2117 76 0.0485409372177088 2118 1 -0.2439481213567518 2118 2 -0.29540854151945695 2118 3 -0.21307577761717056 2118 4 -0.06035381803975781 2118 5 -0.04142861374629092 2118 6 -0.05872373762312577 2118 7 -0.0038764975416683 2118 8 -0.004675126156186593 2118 9 -0.00468389737710595 2118 10-11 0.02416615940970246 2118 12-13 -0.07212158330492713 2118 14-15 -0.01654080805042213 2118 16-17 -0.2626964290264979 2118 18-19 -0.31087114431741725 2118 20-21 0.005856600681973134 2118 22-23 -0.15652124778053889 2118 24-25 -0.10627569098677725 2118 26-27 -0.2276151134610629 2118 28-29 -0.16373969539304056 2118 30-31 -0.048071077814618945 2118 32-33 -0.0182710375916173 2118 34-35 -0.1766409190046474 2118 36-37 0.02990333185791627 2118 38-39 -0.07277336674204804 2118 40-41 -0.3035958593778645 2118 42-43 -0.3154554447779887 2118 44-45 -0.23711434815817256 2118 46-47 -0.07250384252926523 2118 48-49 -0.04332110313973914 2118 50-51 -0.03131855502152803 2118 52-53 -0.0032377462844053184 2118 54-55 -0.20506031884317366 2118 56-57 -0.5010149303884077 2118 58-59 -0.2170110348925789 2118 60-61 -0.28802223552295914 2118 62-63 -0.24369856701214587 2118 64-65 -0.49223685932116723 2118 66-67 -0.3128959821960393 2118 68-69 -0.20004614588478375 2118 70-71 -0.2643782064596394 2118 72-73 -0.25095832264334916 2118 74-75 -0.04743270466978089 2118 76 -0.5379294427681316 2119 1 0.09368105432216822 2119 2 0.15147922584416307 2119 3 0.07997276840542611 2119 4 0.06747002238321187 2119 5 -0.02036997399623175 2119 6 0.09499532029563795 2119 7 -0.022982481680095646 2119 8 0.11206363856383206 2119 9 0.051132012259998305 2119 10-11 0.1101280676048475 2119 12-13 0.07016692907190958 2119 14-15 -0.09107217070363305 2119 16-17 0.03906396510952703 2119 18-19 0.05962272734804941 2119 20-21 -0.014796487517976686 2119 22-23 0.19485161931871886 2119 24-25 0.13523776731651083 2119 26-27 0.2161634253517306 2119 28-29 0.15349173935822336 2119 30-31 -0.016498138617301095 2119 32-33 -0.1392605833738827 2119 34-35 0.23198981879875902 2119 36-37 0.15299573752962914 2119 38-39 -0.05757269382688435 2119 40-41 -0.06720436694816101 2119 42-43 0.04779858069665721 2119 44-45 -0.013970873032000952 2119 46-47 0.032368784243139714 2119 48-49 0.10297786345482507 2119 50-51 0.16242994293569524 2119 52-53 0.13301255412415713 2119 54-55 0.22185027957066694 2119 56-57 0.23013443274545153 2119 58-59 0.11271739577312445 2119 60-61 0.15162568564983303 2119 62-63 -0.05905244829068579 2119 64-65 -0.09436074780951031 2119 66-67 -0.18878218743516584 2119 68-69 0.12226219345672007 2119 70-71 0.16567166136059797 2119 72-73 0.18768322986806396 2119 74-75 0.17719779280678694 2119 76 -1.221449029405953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 3.0 20 1.0 21 7.0 22 7.0 23 20.0 24 53.0 25 70.0 26 156.0 27 253.0 28 457.0 29 773.0 30 1285.0 31 1905.0 32 2918.0 33 4973.0 34 8083.0 35 13784.0 36 23093.0 37 119954.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.82114232683709 15.929581821761015 11.426080598442026 32.82319525295987 2 21.53491380522512 18.136055569616694 33.05885992294497 27.270170702213225 3 20.714305801625468 17.70690964312832 29.017688911386706 32.5610956438595 4 16.97685536713631 19.79864450631345 29.469332658398717 33.75516746815152 5 18.254169127365785 28.757276638825612 34.16687758373407 18.821676650074522 6 32.886751595939145 33.18934728198206 15.426193087544643 18.497708034534156 7 30.81695210776456 23.58671503698079 19.566354509406903 26.02997834584775 8 26.239208076717567 32.83388171770859 16.079192328243202 24.847717877330634 9 24.005174498720436 15.071289968784274 16.841868443994485 44.0816670885008 10-11 29.166174526842713 23.369611068927696 21.71883348800585 25.74538091622374 12-13 27.506679040467958 17.838240670435052 23.335864338142244 31.31921595095475 14-15 24.78978598948227 17.80730616721505 19.42461824010799 37.978289603194696 16-17 29.104024297646163 22.69355156219241 18.82252031834416 29.379903821817262 18-19 30.326218397592736 21.604938271604937 19.427430467673446 28.641412863128885 20-21 26.87167805618831 20.479484799910008 19.30509856857617 33.34373857532552 22-23 27.05250428864704 22.61255940830732 19.141989369779804 31.19294693326584 24-25 29.827610450237636 23.21831322590624 18.998284541185072 27.955791782671053 26-27 29.320565820186168 24.477909952473354 19.51882786355072 26.68269636378976 28-29 29.810455862088357 21.1580753114542 19.90719649034 29.12427233611744 30-31 29.666469810737084 22.963244185719507 20.16451531257909 27.205770690964314 32-33 26.698163615399757 23.029050310751146 20.580724992266376 29.69206108158272 34-35 27.941730644843783 21.016057819398746 20.8951320340842 30.147079501673275 36-37 27.725189122303778 21.325402851598753 23.781883630023344 27.167524396074132 38-39 24.5276863803819 21.71377147838803 25.609550324812286 28.148991816417784 40-41 25.136533648302823 18.890576225428163 25.648921510728652 30.32396861554037 42-43 27.62338648443432 18.70974999296943 25.088163334177 28.578700188419248 44-45 23.912652211816983 22.14488596417222 27.301667650946314 26.640794173064485 46-47 25.98132680896538 20.081273376641636 25.94420540510138 27.9931944092916 48-49 25.56399223825192 19.45920863916308 23.70032903062516 31.27647009195984 50-51 23.8836862678928 20.308782586686917 25.848027222362834 29.95950392305745 52-53 21.43142383081639 20.09477206895582 30.698276104502376 27.77552799572541 54-55 21.484856154560028 20.703900559633283 28.015411007058695 29.795832278747998 56-57 23.93993081920189 21.365898928541295 24.444444444444443 30.249725807812368 58-59 22.38645631204477 21.580753114542027 27.4203436542085 28.612446919204704 60-61 24.445006889957536 23.709328158834612 24.03020332405298 27.815461627154868 62-63 21.78267105374167 28.918417278326164 21.39289631316966 27.90601535476251 64-65 21.25369104867966 30.49326471498074 20.422677803087826 27.83036643325178 66-67 21.484293709046938 29.917882955088725 20.133862032115637 28.463961303748697 68-69 21.404426446188026 30.71149357406001 20.21879130459237 27.665288675159594 70-71 21.883067577828395 29.892572906999636 20.124862903906184 28.099496611265785 72-73 22.255125284738043 28.242638994347423 20.09533451446891 29.406901206445625 74-75 22.149947973790038 27.581765516465595 20.764082229534015 29.504204280210356 76 22.69917601732332 27.668382125481592 20.272786073849097 29.35965578334599 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.5 15 1.0 16 3.0 17 5.0 18 4.0 19 4.0 20 7.5 21 10.0 22 20.5 23 44.5 24 59.0 25 60.0 26 127.5 27 233.0 28 376.5 29 482.0 30 574.5 31 726.5 32 1035.5 33 1285.0 34 1492.0 35 1934.5 36 2370.5 37 2571.0 38 2991.0 39 3928.0 40 4903.5 41 7307.0 42 9252.0 43 10305.0 44 10951.5 45 9542.5 46 8540.0 47 8114.5 48 7246.5 49 6278.5 50 5753.0 51 5376.5 52 4584.0 53 3880.5 54 3593.0 55 3544.0 56 3332.5 57 3158.0 58 3146.0 59 3338.5 60 3810.0 61 4490.0 62 4891.0 63 5155.0 64 5710.5 65 6113.5 66 6425.0 67 6625.0 68 6559.0 69 6266.5 70 5782.5 71 5525.0 72 5292.5 73 4578.0 74 3628.0 75 3160.0 76 2662.0 77 1790.5 78 1058.0 79 699.0 80 462.0 81 153.0 82 48.0 83 15.0 84 9.0 85 2.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 177795.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.22073174161253 #Duplication Level Percentage of deduplicated Percentage of total 1 64.60485419142518 31.798981973621306 2 20.254365115641285 19.93869343907309 3 7.009324435505988 10.350122331899097 4 2.7413383307432126 5.397227143620461 5 1.3632416125788462 3.354987485587334 6 0.9084468415760124 2.682865097443685 7 0.6593381479111436 2.271717427374223 8 0.4822195813145626 1.8988160521949435 9 0.37366304049730326 1.6552771450265755 >10 1.580354694213365 11.579065778002755 >50 0.010284303866898254 0.3003459039905509 >100 0.00685620257793217 0.5348856829494643 >500 0.0011427004296553616 0.285722320650187 >1k 0.003428101288966085 3.596839056216429 >5k 0.0011427004296553616 4.354453162349897 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 7742 4.354453162349897 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 2330 1.310498045501842 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 2090 1.1755111223600214 No Hit CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG 1975 1.1108298883545655 TruSeq Adapter, Index 2 (95% over 24bp) TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 508 0.285722320650187 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 263 0.14792316994291177 TruSeq Adapter, Index 2 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT 204 0.11473888467054753 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006749346157091032 0.0 2 0.0 0.0 0.0 0.009561573722545628 0.0 3 0.0 0.0 0.0 0.012936246801091145 0.0 4 0.0 0.0 0.0 0.08324193593745606 0.0 5 0.0 0.0 0.0 0.09167861863381985 0.0 6 0.0 0.0 0.0 0.09280350966000168 0.0 7 0.0 0.0 0.0 0.09392840068618352 0.0 8 0.0 0.0 0.0 0.09561573722545628 0.0 9 0.0 0.0 0.0 0.10686464748727467 0.0 10 0.0 0.0 0.0 0.10967687505272927 0.0 11 0.0 0.0 0.0 0.11080176607891111 0.0 12 0.0 0.0 0.0 0.11136421159200202 0.0 13 0.0 0.0 0.0 0.11192665710509295 0.0 14 0.0 0.0 0.0 0.11305154813127478 0.0 15 0.0 0.0 0.0 0.11586377569672938 0.0 16 0.0 0.0 0.0 0.11867600326218397 0.0 17 0.0 0.0 0.0 0.1220506763407295 0.0 18 0.0 0.0 0.0 0.125425349419275 0.0 19 0.0 0.0 0.0 0.12880002249782052 0.0 20 0.0 0.0 0.0 0.1338620321156388 0.0 21 0.0 0.0 0.0 0.13723670519418432 0.0 22 0.0 0.0 0.0 0.14061137827272982 0.0 23 0.0 5.624455130909193E-4 0.0 0.14679827891672995 0.0 24 0.0 5.624455130909193E-4 0.0 0.14961050648218455 0.0 25 0.0 5.624455130909193E-4 0.0 0.15748474366545742 0.0 26 0.0 5.624455130909193E-4 0.0 0.18448212829382155 0.0 27 0.0 5.624455130909193E-4 0.0 0.30428302258218737 0.0 28 0.0 5.624455130909193E-4 0.0 0.4280210354621896 0.0 29 0.0 5.624455130909193E-4 0.0 0.5568210579600101 0.0 30 0.0 0.0011248910261818386 0.0 0.7008071093112854 0.0 31 0.0 0.0011248910261818386 0.0 0.8639163081076521 0.0 32 0.0 0.0011248910261818386 0.0 1.0433364267836553 0.0 33 0.0 0.0011248910261818386 0.0 1.2705644140723866 0.0 34 0.0 0.0011248910261818386 0.0 1.5534745071571192 0.0 35 0.0 0.0011248910261818386 0.0 1.9623723951742176 0.0 36 0.0 0.0011248910261818386 0.0 2.588936696757502 0.0 37 0.0 0.0011248910261818386 0.0 3.44441632216879 0.0 38 0.0 0.0011248910261818386 0.0 4.322393768103715 0.0 39 0.0 0.0011248910261818386 0.0 5.170561601844821 0.0 40 0.0 0.0011248910261818386 0.0 6.046851711240474 0.0 41 0.0 0.0011248910261818386 0.0 6.987823054641582 0.0 42 0.0 0.0011248910261818386 0.0 7.973790039089963 0.0 43 0.0 0.0011248910261818386 0.0 8.881014651705616 0.0 44 0.0 0.0011248910261818386 0.0 9.774740572007087 0.0 45 0.0 0.0011248910261818386 0.0 10.765769566073287 0.0 46 0.0 0.0011248910261818386 0.0 11.74105008577294 0.0 47 0.0 0.0011248910261818386 0.0 12.62690176889114 0.0 48 0.0 0.0011248910261818386 0.0 13.475069602632246 0.0 49 0.0 0.0011248910261818386 0.0 14.301302061362806 0.0 50 0.0 0.0011248910261818386 0.0 15.084226215585366 0.0 51 0.0 0.0011248910261818386 0.0 15.886835962766106 0.0 52 0.0 0.0011248910261818386 0.0 16.67763435417194 0.0 53 0.0 0.0011248910261818386 0.0 17.501054585337045 0.0 54 0.0 0.0011248910261818386 0.0 18.300852104952334 0.0 55 0.0 0.0011248910261818386 0.0 19.01684524311707 0.0 56 0.0 0.0011248910261818386 0.0 19.728338817177086 0.0 57 0.0 0.0011248910261818386 0.0 20.448269073933464 0.0 58 0.0 0.0011248910261818386 0.0 21.096206305014203 0.0 59 0.0 0.0011248910261818386 0.0 21.818386343822944 0.0 60 0.0 0.0011248910261818386 0.0 22.491071177479682 0.0 61 0.0 0.0011248910261818386 0.0 23.16375601113642 0.0 62 0.0 0.0011248910261818386 0.0 23.815630360808797 0.0 63 0.0 0.0011248910261818386 0.0 24.51025056947608 0.0 64 0.0 0.0011248910261818386 0.0 25.18293540313282 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTATA 65 0.0 59.230766 2 TTTATAC 90 0.0 58.33333 3 ACAAAAG 20 0.0069424207 52.5 57 CTGTAGA 20 0.0069424207 52.5 1 CTATAAC 20 0.0069424207 52.5 2 CTTGCAT 20 0.0069424207 52.5 13 TGTATAA 30 5.865926E-4 46.666664 11 GGTTTTC 35 0.0012529077 40.0 37 CATTACC 35 0.0012529077 40.0 29 CCAGTAG 35 0.0012529077 40.0 3 CATACAG 45 9.170543E-5 38.888885 5 CCCGACA 45 9.170543E-5 38.888885 21 CTTGAAA 1630 0.0 35.858894 57 TTGAAAA 1635 0.0 35.749233 58 TATGCCG 1675 0.0 35.731346 43 CGTATGC 1685 0.0 35.519287 41 CTCGTAT 1685 0.0 35.519287 39 TCGTATG 1685 0.0 35.519287 40 TGAAAAA 1655 0.0 35.31722 59 GTATGCC 1700 0.0 35.205883 42 >>END_MODULE