FastQCFastQC Report
Wed 25 May 2016
SRR1780231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916909
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGA407.08454E-427.4642144
AACCGTA450.001398339224.44329310
CTAGCGA450.00140860124.4126363
GTACGAT500.002555409222.0362620
ACCGTAT803.615436E-621.99896211
ATCGGTT500.00258476521.99295825
GTCCTTA500.002597900821.9737661
AAGGATG856.1800256E-620.6789386
TATCGGC550.004447302320.02748915
TATCGGT550.004461394620.01654424
TGTCTAG550.00449110819.99359926
GCCTTAC550.004516705419.9739747
CGCAAAG708.0821814E-418.87067812
ACACAGG708.1926887E-418.8326036
CGTCATA600.0074619418.3114742
ATAGGTC600.007466635718.3094753
GTATACC750.001300910717.5790141
GGCATAG750.001300910717.5790141
TAGATCT2055.456968E-1217.18493539
AAGTCAA902.2154307E-417.11030411
Produced by FastQC (version 0.11.5)