FastQCFastQC Report
Wed 25 May 2016
SRR1780231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916909
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT218072.3783167140904933No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC72920.7952806658021678No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG57030.6219810253798359No Hit
CACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTTCTG43890.4786734561445029RNA PCR Primer, Index 31 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC12260.1337101064554934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCT250.002351710335.202444
TCTTCTG6200.032.64738544
GTCTTCT6200.032.29252243
ATGCCGT30500.031.66773444
CCGTCTT6850.029.22667741
CTTTATA1750.028.9162562
TATGCCG34550.027.82823643
CGTCTTC7400.027.64902542
TTCACTA508.344686E-526.39748230
CGCCAAA450.001398733724.44211217
GCCGTCT8650.023.65221240
CGAATCA500.002581396621.997935
GTATGCC44000.021.85029842
AATTCGT44050.021.77317829
CTCGTAT44200.021.75024239
TCGTATG44250.021.72566640
TCGTTAT43900.021.69724732
CGTATGC44350.021.6778641
GTTATCT44150.021.6242134
CAATTCG44400.021.60154228
Produced by FastQC (version 0.11.5)