FastQCFastQC Report
Wed 25 May 2016
SRR1780230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115386
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC200.00693627152.53
CTATAAT200.00693627152.539
GCTACAG200.00693627152.53
TACAGGT200.00693627152.57
TAAGTAG305.8573135E-446.66666433
TAAGGTT305.8573135E-446.6666645
AACGTGG350.001251078640.00000462
AGTGATA350.001251078640.00000443
ACGTGGT350.001251078640.00000463
GTGATAT400.002410451635.044
AGAACGT400.002410451635.060
TTGAGTC400.002410451635.023
TGGTTAC400.002410451635.066
AGGTTGT400.002410451635.07
CAATGCC400.002410451635.024
ACTCCGG400.002410451635.011
TAGTGAT400.002410451635.04
ATACAAT400.002410451635.021
AGAGACA501.7009799E-435.06
TCAGGCC552.976907E-431.8181833