##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780230_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 115386 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.716083406999115 34.0 32.0 34.0 21.0 34.0 2 30.779045984781515 34.0 32.0 34.0 21.0 34.0 3 30.68248314353561 34.0 32.0 34.0 21.0 34.0 4 30.49895134591718 34.0 32.0 34.0 21.0 34.0 5 30.3963045776784 34.0 31.0 34.0 21.0 34.0 6 33.67242993084083 37.0 34.0 38.0 21.0 38.0 7 33.84794515799144 38.0 34.0 38.0 21.0 38.0 8 33.82638275007366 38.0 34.0 38.0 21.0 38.0 9 34.19770162758047 38.0 35.0 38.0 21.0 38.0 10-11 33.95618185915102 38.0 34.5 38.0 21.0 38.0 12-13 34.02643734941847 38.0 34.5 38.0 21.0 38.0 14-15 34.02674067911185 38.0 34.5 38.0 21.0 38.0 16-17 34.097264832822006 38.0 35.0 38.0 21.0 38.0 18-19 34.10714471426343 38.0 35.0 38.0 21.5 38.0 20-21 34.12456450522593 38.0 35.0 38.0 21.5 38.0 22-23 34.131909417086995 38.0 35.0 38.0 21.5 38.0 24-25 34.15707278179329 38.0 35.0 38.0 21.5 38.0 26-27 34.12027022342399 38.0 35.0 38.0 21.5 38.0 28-29 34.229477579602374 38.0 36.0 38.0 22.0 38.0 30-31 34.27126774478706 38.0 36.0 38.0 22.0 38.0 32-33 34.31834884648051 38.0 36.0 38.0 22.0 38.0 34-35 34.28187995077393 38.0 36.0 38.0 22.5 38.0 36-37 34.1974762969511 38.0 36.0 38.0 22.0 38.0 38-39 34.21581474355641 38.0 36.0 38.0 22.0 38.0 40-41 34.27720000693325 38.0 36.0 38.0 22.5 38.0 42-43 34.33017437124088 38.0 36.0 38.0 23.0 38.0 44-45 34.379482779539984 38.0 36.0 38.0 23.0 38.0 46-47 34.33928292860486 38.0 36.0 38.0 22.5 38.0 48-49 34.295512453850556 38.0 36.0 38.0 22.0 38.0 50-51 34.242689754389616 38.0 36.0 38.0 22.0 38.0 52-53 34.295872116201274 38.0 36.0 38.0 23.0 38.0 54-55 34.274942367358264 38.0 36.0 38.0 22.5 38.0 56-57 34.22592428890853 38.0 36.0 38.0 22.0 38.0 58-59 34.285992234759846 38.0 36.0 38.0 23.0 38.0 60-61 34.3085339642591 38.0 36.0 38.0 23.0 38.0 62-63 34.18695508987226 38.0 36.0 38.0 22.0 38.0 64-65 34.18126115819943 38.0 36.0 38.0 22.5 38.0 66-67 34.08917892985284 38.0 35.0 38.0 22.0 38.0 68-69 34.010157211446796 38.0 35.0 38.0 22.0 38.0 70-71 34.04718510044546 38.0 35.0 38.0 22.0 38.0 72-73 34.07026849011145 38.0 35.0 38.0 22.0 38.0 74-75 34.094378867453585 38.0 35.0 38.0 22.0 38.0 76 32.60307142980951 37.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.8882652719185451 1101 2 -0.4054309490322048 1101 3 3.37957923427723E-4 1101 4 0.2232064471621662 1101 5 -0.3248305534086171 1101 6 -0.30206746253269756 1101 7 -1.535260311711994 1101 8 -0.9415820580864676 1101 9 -0.8522488011139799 1101 10-11 -1.1032963673525629 1101 12-13 -0.7461404183122511 1101 14-15 -0.7686219229675544 1101 16-17 -0.779398385317208 1101 18-19 -0.6997992924235987 1101 20-21 -0.8266382096543552 1101 22-23 -0.5311884170490586 1101 24-25 -0.5315191469591696 1101 26-27 -0.9994417841569998 1101 28-29 -0.6989010625062377 1101 30-31 -0.6752080461817869 1101 32-33 -0.8251089897981174 1101 34-35 -0.5969111079035585 1101 36-37 -0.8110399958480912 1101 38-39 -0.6240089858399003 1101 40-41 -0.5163264469756541 1101 42-43 -1.229249358991055 1101 44-45 -0.3609104844199962 1101 46-47 -0.29012685960066875 1101 48-49 -0.8408129298495481 1101 50-51 -0.5047690274855796 1101 52-53 -0.8033306954082562 1101 54-55 -0.6161373264375882 1101 56-57 -0.5113524874634976 1101 58-59 -0.35357863953625923 1101 60-61 -0.32464027669058737 1101 62-63 -0.9199186542067181 1101 64-65 -0.6073205804270714 1101 66-67 -0.9827889761365611 1101 68-69 -0.9264249645679072 1101 70-71 -0.9042463559520613 1101 72-73 -0.43841735976344864 1101 74-75 -0.6697478191517305 1101 76 -1.4121034206414471 1102 1 -0.036047409051398915 1102 2 -0.15425343150464954 1102 3 0.05865826235718785 1102 4 -0.013083708889634948 1102 5 -0.4480986930784212 1102 6 0.019918436756363178 1102 7 -0.7999583854014816 1102 8 -0.34671691948847183 1102 9 -0.03374874703175834 1102 10-11 -0.6387153079309442 1102 12-13 -0.44558582968131333 1102 14-15 -0.6532743709257076 1102 16-17 -0.2358474447781589 1102 18-19 -0.8961718501195008 1102 20-21 -0.51185016280931 1102 22-23 -0.3319495014468359 1102 24-25 -0.3109177526210942 1102 26-27 -0.6052364486997064 1102 28-29 -0.5622991961778325 1102 30-31 -0.8106741857925996 1102 32-33 -0.3363531759589691 1102 34-35 -0.4969917395839403 1102 36-37 -0.006773399979977057 1102 38-39 -0.3570197924516947 1102 40-41 -0.40423857811029507 1102 42-43 -0.3858862312685005 1102 44-45 -0.2014170873679717 1102 46-47 -0.7890697979370955 1102 48-49 -0.8089290001367857 1102 50-51 -0.6196790051643717 1102 52-53 -0.13975654867827103 1102 54-55 -1.0315527270885454 1102 56-57 -0.4041910176069621 1102 58-59 -0.9094701604267499 1102 60-61 -0.4969855769452707 1102 62-63 -0.4932836502243063 1102 64-65 -0.4872777079726234 1102 66-67 -0.46637256267304394 1102 68-69 -0.4433674505802827 1102 70-71 -0.2562970555793882 1102 72-73 -0.2727389228380801 1102 74-75 -1.0377675247477711 1102 76 -0.6294942974618039 1103 1 0.7345947927834615 1103 2 0.3686823483118644 1103 3 -0.14317660002813426 1103 4 0.7239193492143414 1103 5 0.3928799001937513 1103 6 -0.39598370391641424 1103 7 0.8147205136810882 1103 8 0.014139349624677777 1103 9 0.5778720480752781 1103 10-11 0.07535197035651464 1103 12-13 0.25624903270400523 1103 14-15 0.40330055262169395 1103 16-17 0.5240913931423279 1103 18-19 0.15581591746460077 1103 20-21 0.3749999900958869 1103 22-23 0.10535936705469595 1103 24-25 0.5132412687856345 1103 26-27 0.08742410175900517 1103 28-29 -0.0011371166671239052 1103 30-31 -0.06602586774977937 1103 32-33 0.37618505034072314 1103 34-35 0.030531196195873633 1103 36-37 0.08496971928607877 1103 38-39 -0.2912705569776932 1103 40-41 0.04071555033007712 1103 42-43 0.38628502255412656 1103 44-45 0.5845156343445623 1103 46-47 0.1727039023687169 1103 48-49 0.16201901209563374 1103 50-51 -0.009587262045101852 1103 52-53 0.6110079773156158 1103 54-55 0.18557265517446098 1103 56-57 0.23342366129212166 1103 58-59 -0.018032851558245966 1103 60-61 0.3057667166327178 1103 62-63 0.4470833222527588 1103 64-65 0.47755409630871526 1103 66-67 0.10090572478873128 1103 68-69 0.2569383598172763 1103 70-71 0.14584361720348937 1103 72-73 0.21349960927201295 1103 74-75 0.17018354558862114 1103 76 0.8261631948929349 1104 1 -0.1932034620346208 1104 2 -0.07697577180615411 1104 3 -0.5762525504168998 1104 4 0.12313195470099458 1104 5 0.14561291247100527 1104 6 -0.04021231736669506 1104 7 0.07055198614470015 1104 8 -0.34414309004279175 1104 9 0.24443109817975284 1104 10-11 0.4217293278318337 1104 12-13 0.04297991058611217 1104 14-15 0.2017885399229371 1104 16-17 -0.06069700579226378 1104 18-19 0.09026397902127314 1104 20-21 -0.5659947456606105 1104 22-23 -0.044653636613233516 1104 24-25 -0.07127360472006217 1104 26-27 0.03137829297569539 1104 28-29 -0.10578077132557695 1104 30-31 -0.29102967916968936 1104 32-33 -0.07718110604407968 1104 34-35 0.16870637802302468 1104 36-37 0.013530258086333902 1104 38-39 0.07891696488192679 1104 40-41 -0.02849983525110389 1104 42-43 -0.0660542924664611 1104 44-45 -0.14012048715453318 1104 46-47 -0.16634207680748148 1104 48-49 0.09243145257022434 1104 50-51 -0.3558076787005575 1104 52-53 -0.1569482944879539 1104 54-55 -0.058627260525902614 1104 56-57 0.09554890903442015 1104 58-59 -0.0743073455362051 1104 60-61 0.29913036196440146 1104 62-63 -0.020672065301383213 1104 64-65 0.09238993621114844 1104 66-67 0.05350959719136483 1104 68-69 0.09553145262758989 1104 70-71 0.08391028566376946 1104 72-73 0.32831751308074075 1104 74-75 0.1269396236823681 1104 76 0.4829470739319177 1105 1 -0.187112082287328 1105 2 0.14776265185700055 1105 3 0.614327081022946 1105 4 0.3770510742115114 1105 5 0.4274817803613793 1105 6 0.33176009492829905 1105 7 0.41068268380227835 1105 8 -0.2315566521875354 1105 9 0.0170497578951867 1105 10-11 -0.03396108888009053 1105 12-13 -0.4403615687231124 1105 14-15 0.3962782397889839 1105 16-17 0.046630752073127724 1105 18-19 0.059598905345445985 1105 20-21 0.27582397922262913 1105 22-23 0.16421371069497326 1105 24-25 -0.14296271807584304 1105 26-27 0.4842130925846959 1105 28-29 0.2919736546546545 1105 30-31 0.2209403798433769 1105 32-33 -0.33847685927531046 1105 34-35 0.27659530311625957 1105 36-37 0.1844147300460719 1105 38-39 0.08721228366111689 1105 40-41 0.19345085444152232 1105 42-43 0.17480863791260504 1105 44-45 0.22898273141105108 1105 46-47 0.0659675788892713 1105 48-49 0.13373155032193296 1105 50-51 0.2115731185189489 1105 52-53 0.2611665456699015 1105 54-55 0.21595548448694757 1105 56-57 0.17413042309979687 1105 58-59 -0.01592332613068237 1105 60-61 0.15845388392232707 1105 62-63 0.2589249804246947 1105 64-65 -0.06906783142942174 1105 66-67 0.32918752340692237 1105 68-69 0.3098747059493405 1105 70-71 0.23212179140215738 1105 72-73 0.20920976897300392 1105 74-75 0.2066117934083067 1105 76 0.012254484959761669 1106 1 -0.21952423803281462 1106 2 -0.43045197302429017 1106 3 0.1797591797883804 1106 4 0.9190263828534846 1106 5 0.40972299593592965 1106 6 0.7102501234183265 1106 7 -0.7471425773563141 1106 8 -0.40864117260538535 1106 9 0.1333460541914846 1106 10-11 -0.4152478885002253 1106 12-13 0.2101939868324436 1106 14-15 0.1335052103492913 1106 16-17 0.11086626964197421 1106 18-19 -0.10402882494894783 1106 20-21 0.31318865158730347 1106 22-23 0.08344922826382373 1106 24-25 0.11096415751769939 1106 26-27 -0.12681254844094525 1106 28-29 0.08443804211903938 1106 30-31 -0.057354966785304384 1106 32-33 0.07556872477027099 1106 34-35 -0.31105901787140056 1106 36-37 -0.24586067356266028 1106 38-39 0.01719803864686753 1106 40-41 -0.06649216550150072 1106 42-43 -0.011188513084547935 1106 44-45 0.08565889475388389 1106 46-47 -0.10900393108223483 1106 48-49 -0.46601203942165625 1106 50-51 -0.5082416962958689 1106 52-53 -0.09893791776789129 1106 54-55 -0.14090111570298092 1106 56-57 0.017606206575578653 1106 58-59 -0.5366308285048476 1106 60-61 -0.221370427568651 1106 62-63 -0.49027729877757764 1106 64-65 -0.1708674515623727 1106 66-67 -0.38122083366810244 1106 68-69 -0.6848024070611061 1106 70-71 -0.43014791420088727 1106 72-73 -0.17447968971645622 1106 74-75 -0.18816978303993892 1106 76 0.213432072804018 1107 1 -0.20886757762005814 1107 2 -0.6226001906603145 1107 3 -1.083655454357963 1107 4 -0.9626809342196836 1107 5 -0.758757304251688 1107 6 -1.8693464994709785 1107 7 -0.9069174366433685 1107 8 -0.7443916416485408 1107 9 -0.6653124823938796 1107 10-11 -0.7616221849354972 1107 12-13 -0.2918182082895058 1107 14-15 -0.4394075476244481 1107 16-17 -0.683533355123501 1107 18-19 -0.8602670988701462 1107 20-21 -0.6279135998198271 1107 22-23 -0.5019260062577544 1107 24-25 -0.47343546724778207 1107 26-27 -0.34302267977302847 1107 28-29 -0.5473443218584464 1107 30-31 -0.8180350793556599 1107 32-33 -0.44286936153367407 1107 34-35 -0.2979492617738444 1107 36-37 -0.9172865647446571 1107 38-39 -0.970771004505103 1107 40-41 -0.7026647733814215 1107 42-43 -0.7590215337224464 1107 44-45 -1.1332763772911463 1107 46-47 -0.670199991119766 1107 48-49 -0.6320204821983921 1107 50-51 -0.3944002328812317 1107 52-53 -0.3490035838091714 1107 54-55 -0.48804933333900635 1107 56-57 -0.6047765326364214 1107 58-59 -0.45470306115025494 1107 60-61 -0.7676790579626456 1107 62-63 -0.40712063836482315 1107 64-65 -0.6082126673222206 1107 66-67 -0.6190773064635948 1107 68-69 -0.5089675102505993 1107 70-71 -0.39272765153672395 1107 72-73 -0.3999252807108249 1107 74-75 -0.598924942514607 1107 76 -0.7940538559014243 1108 1 -0.51210747569068 1108 2 -0.7272143151597668 1108 3 -0.10680798439072348 1108 4 0.03245921867438284 1108 5 -0.13218285940276076 1108 6 -0.06793586969303789 1108 7 -0.5123779390095891 1108 8 -0.46163772828850824 1108 9 -0.470236035360756 1108 10-11 -0.2730664878113558 1108 12-13 -0.4933881027197913 1108 14-15 -0.3437622982615025 1108 16-17 -0.9007066350652551 1108 18-19 -0.3955328433186409 1108 20-21 -0.5522992933036264 1108 22-23 -0.197675915249377 1108 24-25 -0.4674744188313298 1108 26-27 -0.581209792987444 1108 28-29 -0.6541153447925581 1108 30-31 -0.5175845879104486 1108 32-33 -0.24679637281870725 1108 34-35 -0.31105901787140056 1108 36-37 -0.5640466666740309 1108 38-39 -0.31070092805806837 1108 40-41 -0.1447470449504067 1108 42-43 -0.2921758839226669 1108 44-45 -0.364697017990089 1108 46-47 -0.6415642066275851 1108 48-49 -0.6277112357477179 1108 50-51 -0.4222715470421363 1108 52-53 -0.42353034027076575 1108 54-55 -0.36094703992801414 1108 56-57 -0.23653845473325674 1108 58-59 -0.4130257768400938 1108 60-61 -0.327730932735129 1108 62-63 -0.3736068050921588 1108 64-65 -0.028571240310938606 1108 66-67 0.027068488949581138 1108 68-69 -0.539291500057665 1108 70-71 -0.22344297734669283 1108 72-73 -0.4570284842055443 1108 74-75 -0.5645647313751851 1108 76 -0.43488021192617765 1109 1 -0.20730123168137027 1109 2 -0.07780083211627087 1109 3 -0.08507262449296604 1109 4 -0.037548540693915555 1109 5 0.03202363108060169 1109 6 0.0749643083159981 1109 7 0.579246507564882 1109 8 -0.17240499288296718 1109 9 0.16502801137802692 1109 10-11 0.5707648143932147 1109 12-13 -0.11225249634798473 1109 14-15 0.2106400021629824 1109 16-17 0.669351331980252 1109 18-19 0.2811981804615371 1109 20-21 0.13646788000414034 1109 22-23 0.5075000398375735 1109 24-25 0.1049843880516903 1109 26-27 0.46204183876206883 1109 28-29 0.07837358669678451 1109 30-31 0.31623712919211044 1109 32-33 0.4694572215945456 1109 34-35 0.5182987802937404 1109 36-37 0.1981195663808819 1109 38-39 0.28059488644738906 1109 40-41 0.030012186415703468 1109 42-43 0.18292110818103424 1109 44-45 0.032527866760467816 1109 46-47 0.35007393294975486 1109 48-49 0.1910709998632214 1109 50-51 0.2855197715940392 1109 52-53 -0.07553636519202911 1109 54-55 0.09994574385947175 1109 56-57 -0.06321242433479313 1109 58-59 0.5752393196955765 1109 60-61 0.27977283284067056 1109 62-63 0.1886735363200387 1109 64-65 0.09223299539128504 1109 66-67 0.2109974679080011 1109 68-69 0.5841554851995312 1109 70-71 0.7314705285184786 1109 72-73 0.0667106184463293 1109 74-75 0.30626917249993824 1109 76 0.015765641376113138 1110 1 0.6383123004287228 1110 2 0.3373845654372474 1110 3 0.9578841797883797 1110 4 0.2940263828534846 1110 5 0.040251842089773504 1110 6 0.17851935418755716 1110 7 0.3299728072590682 1110 8 0.19097421200999776 1110 9 0.44147105419148147 1110 10-11 0.03689153457670358 1110 12-13 0.4636314868324476 1110 14-15 0.5309811718877526 1110 16-17 0.8044239619496736 1110 18-19 0.718086559666439 1110 20-21 0.3255924977411482 1110 22-23 0.4141944205715191 1110 24-25 0.5348343498253882 1110 26-27 0.44619225925136163 1110 28-29 0.19289958058057977 1110 30-31 0.056202725522389585 1110 32-33 0.6263379555394977 1110 34-35 0.503003482128598 1110 36-37 0.48397105720655986 1110 38-39 0.33570765403148073 1110 40-41 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0.14952999248543364 2116 7 0.865699757613676 2116 8 0.4003713751305611 2116 9 -0.3816894068014207 2116 10-11 0.3208786799667749 2116 12-13 0.2526053343501715 2116 14-15 0.11561104777427289 2116 16-17 0.30437963570853555 2116 18-19 0.6181752121487065 2116 20-21 0.5257254764645509 2116 22-23 -0.03459768935756102 2116 24-25 -0.012118221096592663 2116 26-27 0.05513507840029774 2116 28-29 0.03261589263731679 2116 30-31 0.441619392189061 2116 32-33 0.1802386647593579 2116 34-35 0.46624594666760544 2116 36-37 0.21724898273848225 2116 38-39 0.3999585405563053 2116 40-41 0.42118923111607387 2116 42-43 0.5111955567463298 2116 44-45 0.48384220080952645 2116 46-47 0.8207942140350539 2116 48-49 0.38905069925810665 2116 50-51 0.4987086583140581 2116 52-53 0.2910457156199939 2116 54-55 0.4893334724039491 2116 56-57 0.12468203854503201 2116 58-59 0.35874833134239026 2116 60-61 0.5116519401345698 2116 62-63 0.30009913330098215 2116 64-65 0.062367682098297905 2116 66-67 0.06703884991073039 2116 68-69 0.4688909917386752 2116 70-71 0.37212704488258197 2116 72-73 0.3629725743315504 2116 74-75 0.7040178874456089 2116 76 0.8630556407254559 2117 1 0.4757876941295116 2117 2 0.12958436858685474 2117 3 0.7183418569537352 2117 4 -0.020343695886673174 2117 5 0.37241227516064157 2117 6 0.9866886455261437 2117 7 0.9324334371803289 2117 8 0.7907773616162928 2117 9 0.3645271565536916 2117 10-11 1.2545219479625302 2117 12-13 0.49609949864346703 2117 14-15 1.0383507585019203 2117 16-17 0.6554574265166053 2117 18-19 1.0400353785640704 2117 20-21 0.5768228127017778 2117 22-23 1.0318986528549843 2117 24-25 1.2986261805340575 2117 26-27 1.036706530904901 2117 28-29 0.8940806829427856 2117 30-31 0.658810993238923 2117 32-33 0.5964166956969805 2117 34-35 1.1196496435459267 2117 36-37 0.7779794233482988 2117 38-39 0.5865196619054984 2117 40-41 0.7257489005190934 2117 42-43 0.7237660599015214 2117 44-45 0.9636964477520635 2117 46-47 0.8616441609831753 2117 48-49 0.7190333875922761 2117 50-51 1.0040417682710654 2117 52-53 0.9004442748426484 2117 54-55 1.1725997928372962 2117 56-57 0.6639962732018745 2117 58-59 1.12618564635887 2117 60-61 0.7883872950237176 2117 62-63 0.880388964093406 2117 64-65 0.6669217670929868 2117 66-67 0.8539517889848369 2117 68-69 0.5929625838534918 2117 70-71 0.7602155019105581 2117 72-73 -0.5351398956522573 2117 74-75 0.6919695264694496 2117 76 0.623316991035388 2118 1 -0.6360424566562983 2118 2 -0.5817070337530375 2118 3 -0.30445134652741146 2118 4 -0.8129574228145309 2118 5 -1.0809652631733826 2118 6 0.0859586254426219 2118 7 -0.40074581622270955 2118 8 0.5924924306334773 2118 9 0.3437104266611186 2118 10-11 -0.7011588196743119 2118 12-13 -0.6596035840177592 2118 14-15 -0.28633284633087186 2118 16-17 -0.568728872058422 2118 18-19 -0.36435271158863003 2118 20-21 -0.5462445467932682 2118 22-23 -0.15104469886168204 2118 24-25 -0.32927875746206325 2118 26-27 -0.3521566779956018 2118 28-29 -0.3453595287311657 2118 30-31 -0.03881245666384103 2118 32-33 -0.5692086031649595 2118 34-35 -0.8304922162519617 2118 36-37 -0.3575205925910012 2118 38-39 -0.24434042289159663 2118 40-41 -0.9135366999144523 2118 42-43 -0.6730508612626807 2118 44-45 -0.5213046679991535 2118 46-47 -0.7795302468717082 2118 48-49 -0.4104654807171997 2118 50-51 -0.3577963521663179 2118 52-53 -0.20193386918489864 2118 54-55 -0.7624395772414445 2118 56-57 -0.3197217286470888 2118 58-59 -0.17703568680444448 2118 60-61 -0.27027731015974155 2118 62-63 -0.6791841813686759 2118 64-65 -0.37054356492269847 2118 66-67 -8.969470284228009E-4 2118 68-69 -0.34682669855908443 2118 70-71 -0.5824556065085815 2118 72-73 -0.20646349538447595 2118 74-75 -0.49821026886989017 2118 76 -0.038227846224323514 2119 1 0.6041405524612422 2119 2 0.3011802971445654 2119 3 -0.14463106411405846 2119 4 -0.17609556836602636 2119 5 1.1070963542848986 2119 6 0.25565350052902147 2119 7 1.0443020755517551 2119 8 0.47207177298560765 2119 9 0.821476135492297 2119 10-11 0.8791298475848279 2119 12-13 1.028811873011307 2119 14-15 0.9178078995300254 2119 16-17 0.7565072952830079 2119 18-19 0.8704239580404121 2119 20-21 0.24861079042407397 2119 22-23 0.39339919699428094 2119 24-25 0.7430279473863663 2119 26-27 0.7016927673814379 2119 28-29 0.7697288488732568 2119 30-31 0.6672291482866157 2119 32-33 0.6938176303362482 2119 34-35 0.5023556162749472 2119 36-37 0.4607622157431521 2119 38-39 0.44233875159245883 2119 40-41 0.6875791290576032 2119 42-43 0.7623199296921825 2119 44-45 1.2320691511641328 2119 46-47 0.34047074058755555 2119 48-49 0.16421935519998954 2119 50-51 0.4363924500455951 2119 52-53 0.42437039993067316 2119 54-55 0.2883734308886332 2119 56-57 0.39725598768878 2119 58-59 0.3781221421017804 2119 60-61 0.117991673744676 2119 62-63 0.48972895513110615 2119 64-65 0.23629952779972996 2119 66-67 0.3631381406908787 2119 68-69 -0.05774799805547559 2119 70-71 0.06560568179661885 2119 72-73 0.8678592721347016 2119 74-75 -0.16853876365281906 2119 76 -0.1660050754136222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 109.0 14 559.0 15 1214.0 16 1660.0 17 1963.0 18 2054.0 19 1830.0 20 1402.0 21 1050.0 22 825.0 23 745.0 24 648.0 25 718.0 26 802.0 27 964.0 28 1154.0 29 1442.0 30 1772.0 31 2252.0 32 3019.0 33 4057.0 34 5475.0 35 8583.0 36 16161.0 37 54926.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.39323661449396 18.809040958175167 10.478740921775605 29.318981505555268 2 21.79553845353856 19.657497443364015 31.889483992858754 26.65748011023868 3 19.08290433848127 22.73066056540655 28.14379560778604 30.042639488326138 4 14.798155755464268 17.541122839859256 31.954483212868112 35.70623819180837 5 14.779089317594856 31.640753644289603 32.99793735808504 20.582219680030505 6 29.056384656717455 35.04757942904685 17.637321685473108 18.25871422876259 7 28.68025583692996 26.984209522819057 21.762605515400484 22.572929124850504 8 25.32369611564661 31.181425822890123 20.829216716066075 22.66566134539719 9 24.314908221101348 17.392924618237913 20.43575477094275 37.85641238971799 10-11 25.735791170505955 23.1418889639991 25.827656734785847 25.2946631307091 12-13 25.057632641741634 21.084880314769556 25.50569393167282 28.351793111815994 14-15 23.930112838645936 22.086301631047096 23.1925883556064 30.790997174700568 16-17 24.413273707382178 24.64770422754927 24.261175532560276 26.677846532508276 18-19 23.518017783786593 24.23734248522351 25.278196661640063 26.966443069349832 20-21 23.74291508501898 24.42540689511726 25.4164283361933 26.41524968367046 22-23 24.753869620231225 24.12077721734006 24.879968107049383 26.245385055379334 24-25 24.20397621895204 24.920700951588582 24.414573691782365 26.460749137677013 26-27 23.162255386268697 25.307662974710972 25.536026901010523 25.994054738009808 28-29 23.764148163555372 24.729169916627665 26.31168425978888 25.19499766002808 30-31 23.829580711698124 24.230842563222573 26.173885913369038 25.765690811710257 32-33 22.884492052762035 25.044632797739762 26.27355138405006 25.797323765448148 34-35 23.570450487927477 24.54500545993448 26.332917338325274 25.55162671381277 36-37 23.618549910734405 23.957845839183264 26.172152600835457 26.251451649246878 38-39 22.60759537552216 24.294541798831748 27.78846653840154 25.30939628724455 40-41 22.71116079940374 23.277087341618568 27.862998977345605 26.148752881632088 42-43 21.570207824172776 23.184788449205275 28.015097152167506 27.22990657445444 44-45 21.138613003310628 22.753193628343126 29.38441405369802 26.723779314648223 46-47 20.99604804742343 23.130189104397413 29.52481236891824 26.348950479260914 48-49 20.386788691869032 23.563950565926543 29.84850848456485 26.200752257639575 50-51 20.392421957603176 24.51770578753055 28.67852252439637 26.4113497304699 52-53 19.388834000658658 24.812368918239645 28.428925519560433 27.36987156154126 54-55 19.45036659560085 24.454006551921374 28.321460142478287 27.77416670999948 56-57 19.536598894146604 24.57663841367237 28.145095592186227 27.7416670999948 58-59 19.91879430780164 24.161076733745862 27.788033210268143 28.132095748184355 60-61 19.641030974294978 23.571750472327665 28.298493751408316 28.488724801969045 62-63 19.801362383651398 24.24644237602482 27.720867349591806 28.23132789073198 64-65 20.773750714991422 24.05881129426447 26.095886849357807 29.071551141386305 66-67 20.526753678955853 24.7564695890316 25.32022949057945 29.3965472414331 68-69 20.650252196973636 25.02469970360356 24.863501637980345 29.461546461442463 70-71 21.023347719827363 25.558993292080494 24.776402683167802 28.64125630492434 72-73 20.810583606330056 26.41481635553707 24.436673426585546 28.337926611547324 74-75 20.554575748252596 27.73572069280802 24.382613066633155 27.327090492306226 76 20.82748340353249 27.949664604024754 23.67184927114208 27.551002721300677 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.0 15 4.0 16 6.0 17 6.0 18 10.0 19 16.5 20 25.5 21 32.0 22 37.0 23 51.5 24 81.5 25 102.0 26 151.5 27 237.0 28 359.5 29 446.0 30 567.5 31 784.5 32 1141.5 33 1403.0 34 1571.5 35 2034.5 36 2585.5 37 2842.0 38 3193.0 39 4078.5 40 4979.0 41 5643.0 42 5941.0 43 6077.5 44 6652.5 45 7101.0 46 7111.0 47 7235.0 48 7036.0 49 6540.5 50 6368.0 51 5871.5 52 4893.0 53 4033.0 54 3655.0 55 3460.5 56 2986.5 57 2576.5 58 2446.0 59 2376.5 60 2249.0 61 2159.0 62 2127.0 63 2058.0 64 1969.5 65 1938.0 66 1911.5 67 1897.0 68 1872.0 69 1728.0 70 1522.5 71 1436.0 72 1262.0 73 926.0 74 643.5 75 523.0 76 376.5 77 170.0 78 75.0 79 40.0 80 28.0 81 12.5 82 7.5 83 6.0 84 4.0 85 3.0 86 4.5 87 5.0 88 4.5 89 4.0 90 4.5 91 4.5 92 4.0 93 3.5 94 4.5 95 5.0 96 4.0 97 2.5 98 2.0 99 16.0 100 29.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.333281333957326E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 115386.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.59846081847018 #Duplication Level Percentage of deduplicated Percentage of total 1 75.9384321008498 46.77690534380254 2 12.932635488772581 15.932608808694296 3 4.416399347177669 8.161302064375226 4 2.1160447971185774 5.2138041010174545 5 1.2212279813157747 3.761288197874959 6 0.8300973605717823 3.067963184441787 7 0.5979514885474703 2.578302393704609 8 0.42067645900163203 2.0730417901651848 9 0.35455005909167653 1.9655764130830429 >10 1.1719849175530417 10.4692077028409 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0025999688003743955 0.0 2 0.0 0.0 0.0 0.004333281333957326 0.0 3 0.0 0.0 0.0 0.004333281333957326 0.0 4 0.0 0.0 0.0 0.010399875201497582 0.0 5 0.0 0.0 0.0 0.011266531468289048 0.0 6 0.0 0.0 0.0 0.011266531468289048 0.0 7 0.0 0.0 0.0 0.012133187735080512 0.0 8 0.0 0.0 0.0 0.012999844001871978 0.0 9 0.0 0.0 0.0 0.015599812802246373 0.0 10 0.0 0.0 0.0 0.01646646906903784 0.0 11 0.0 0.0 0.0 0.018199781602620767 0.0 12 0.0 0.0 0.0 0.019066437869412235 0.0 13 0.0 0.0 0.0 0.024266375470161024 0.0 14 0.0 0.0 0.0 0.02513303173695249 0.0 15 0.0 0.0 0.0 0.025999688003743956 0.0 16 0.0 0.0 0.0 0.02686634427053542 0.0 17 0.0 0.0 0.0 0.03293293813807568 0.0 18 0.0 0.0 0.0 0.03293293813807568 0.0 19 0.0 0.0 0.0 0.0398661882724074 0.0 20 0.0 0.0 0.0 0.045932782139947656 0.0 21 0.0 0.0 0.0 0.04853275094032205 0.0 22 0.0 0.0 0.0 0.0571993136082367 0.0 23 0.0 0.0 0.0 0.06586587627615136 0.0 24 0.0 0.0 0.0 0.07366578267727454 0.0 25 0.0 0.0 0.0 0.08059903281160626 0.0 26 0.0 0.0 0.0 0.09186556427989531 0.0 27 0.0 0.0 0.0 0.18026450349262477 0.0 28 0.0 0.0 0.0 0.26086353630423104 0.0 29 0.0 0.0 0.0 0.3657289445859983 0.0 30 0.0 0.0 0.0 0.4714610091345571 0.0 31 0.0 0.0 0.0 0.5945261990189451 0.0 32 0.0 0.0 0.0 0.7392577955731198 0.0 33 0.0 0.0 0.0 0.8874560171944603 0.0 34 0.0 0.0 0.0 1.1188532404277816 0.0 35 0.0 0.0 0.0 1.4031164959353821 0.0 36 0.0 0.0 0.0 1.8303780354635744 0.0 37 0.0 0.0 0.0 2.4491706099526804 0.0 38 0.0 0.0 0.0 3.2334945313989567 0.0 39 0.0 0.0 0.0 4.023885046712773 0.0 40 0.0 0.0 0.0 4.923474251642314 0.0 41 0.0 0.0 0.0 6.045794117137261 0.0 42 0.0 0.0 0.0 7.368311580261037 0.0 43 0.0 0.0 0.0 8.761028200994922 0.0 44 0.0 0.0 0.0 9.976080287036556 0.0 45 0.0 0.0 0.0 11.095800183731129 0.0 46 0.0 0.0 0.0 12.192120361222331 0.0 47 0.0 0.0 0.0 13.229507912571716 0.0 48 0.0 0.0 0.0 14.190629712443451 0.0 49 0.0 0.0 0.0 15.055552666701333 0.0 50 0.0 0.0 0.0 15.877142807619641 0.0 51 0.0 0.0 0.0 16.796665106685385 0.0 52 0.0 0.0 0.0 17.907718440712046 0.0 53 0.0 0.0 0.0 19.195569653164164 0.0 54 0.0 0.0 0.0 20.456554521345744 0.0 55 0.0 0.0 0.0 21.437609415353684 0.0 56 0.0 0.0 0.0 22.362331652020174 0.0 57 0.0 0.0 0.0 23.236787825212765 0.0 58 0.0 0.0 0.0 23.969979026918345 0.0 59 0.0 0.0 0.0 24.693637009689215 0.0 60 0.0 0.0 0.0 25.45889449326608 0.0 61 0.0 0.0 0.0 26.192952351238453 0.0 62 0.0 0.0 0.0 26.988542804153017 0.0 63 0.0 0.0 0.0 27.8837987277486 0.0 64 0.0 0.0 0.0 28.77645468254381 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAATAC 20 0.006936271 52.5 3 CTATAAT 20 0.006936271 52.5 39 GCTACAG 20 0.006936271 52.5 3 TACAGGT 20 0.006936271 52.5 7 TAAGTAG 30 5.8573135E-4 46.666664 33 TAAGGTT 30 5.8573135E-4 46.666664 5 AACGTGG 35 0.0012510786 40.000004 62 AGTGATA 35 0.0012510786 40.000004 43 ACGTGGT 35 0.0012510786 40.000004 63 GTGATAT 40 0.0024104516 35.0 44 AGAACGT 40 0.0024104516 35.0 60 TTGAGTC 40 0.0024104516 35.0 23 TGGTTAC 40 0.0024104516 35.0 66 AGGTTGT 40 0.0024104516 35.0 7 CAATGCC 40 0.0024104516 35.0 24 ACTCCGG 40 0.0024104516 35.0 11 TAGTGAT 40 0.0024104516 35.0 4 ATACAAT 40 0.0024104516 35.0 21 AGAGACA 50 1.7009799E-4 35.0 6 TCAGGCC 55 2.976907E-4 31.81818 33 >>END_MODULE