Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780230_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 115386 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT | 5402 | 4.681677153207494 | No Hit |
CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG | 1666 | 1.443849340474581 | RNA PCR Primer, Index 23 (95% over 24bp) |
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG | 1493 | 1.2939178063196575 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC | 1341 | 1.1621860537673547 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC | 376 | 0.3258627563135909 | No Hit |
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT | 243 | 0.210597472830326 | RNA PCR Primer, Index 47 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT | 140 | 0.12133187735080511 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT | 122 | 0.10573206454855875 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATCGTAT | 15 | 0.00221982 | 70.0 | 39 |
TGCGGGT | 20 | 0.006936271 | 52.5 | 45 |
CTCGGGC | 20 | 0.006936271 | 52.5 | 2 |
TAGCTTG | 20 | 0.006936271 | 52.5 | 23 |
CGATATG | 20 | 0.006936271 | 52.5 | 24 |
CTTTATA | 50 | 6.136543E-8 | 48.999996 | 2 |
TTAATAC | 45 | 1.6725389E-6 | 46.666664 | 3 |
TCTTTAT | 55 | 1.298904E-7 | 44.545452 | 1 |
TTTATAC | 80 | 2.910383E-11 | 43.75 | 3 |
CTTAATA | 50 | 3.4536351E-6 | 42.0 | 2 |
CGCTCTA | 35 | 0.0012510786 | 40.000004 | 14 |
AAAGACC | 35 | 0.0012510786 | 40.000004 | 69 |
TAATACA | 75 | 3.4950062E-8 | 37.333336 | 4 |
TATGCCG | 1420 | 0.0 | 35.24648 | 43 |
TCTGACT | 40 | 0.0024104516 | 35.0 | 59 |
AACGGCC | 40 | 0.0024104516 | 35.0 | 24 |
ACTACAC | 50 | 1.7009799E-4 | 35.0 | 3 |
ATGCCGT | 1420 | 0.0 | 35.0 | 44 |
GCCGTCT | 1420 | 0.0 | 35.0 | 46 |
TCGTATG | 1425 | 0.0 | 34.87719 | 40 |