FastQCFastQC Report
Wed 25 May 2016
SRR1780230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115386
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT54024.681677153207494No Hit
CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG16661.443849340474581RNA PCR Primer, Index 23 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG14931.2939178063196575No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC13411.1621860537673547No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC3760.3258627563135909No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT2430.210597472830326RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT1400.12133187735080511No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT1220.10573206454855875No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT150.0022198270.039
TGCGGGT200.00693627152.545
CTCGGGC200.00693627152.52
TAGCTTG200.00693627152.523
CGATATG200.00693627152.524
CTTTATA506.136543E-848.9999962
TTAATAC451.6725389E-646.6666643
TCTTTAT551.298904E-744.5454521
TTTATAC802.910383E-1143.753
CTTAATA503.4536351E-642.02
CGCTCTA350.001251078640.00000414
AAAGACC350.001251078640.00000469
TAATACA753.4950062E-837.3333364
TATGCCG14200.035.2464843
TCTGACT400.002410451635.059
AACGGCC400.002410451635.024
ACTACAC501.7009799E-435.03
ATGCCGT14200.035.044
GCCGTCT14200.035.046
TCGTATG14250.034.8771940