##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780230_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 115386 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.705596866170936 34.0 34.0 34.0 34.0 34.0 2 33.690413048376755 34.0 34.0 34.0 34.0 34.0 3 33.72427330872029 34.0 34.0 34.0 34.0 34.0 4 33.75110498674016 34.0 34.0 34.0 34.0 34.0 5 33.743019083770996 34.0 34.0 34.0 34.0 34.0 6 37.49597871492209 38.0 38.0 38.0 37.0 38.0 7 37.504879274782034 38.0 38.0 38.0 37.0 38.0 8 37.39806388989999 38.0 38.0 38.0 37.0 38.0 9 37.40873242854419 38.0 38.0 38.0 37.0 38.0 10-11 37.354557745307055 38.0 38.0 38.0 37.0 38.0 12-13 37.34845648518885 38.0 38.0 38.0 37.0 38.0 14-15 37.28139462326452 38.0 38.0 38.0 36.0 38.0 16-17 37.306089993586745 38.0 38.0 38.0 36.0 38.0 18-19 37.26832544676131 38.0 38.0 38.0 36.0 38.0 20-21 37.299520739084464 38.0 38.0 38.0 36.0 38.0 22-23 37.28529457646508 38.0 38.0 38.0 36.0 38.0 24-25 37.29331981349557 38.0 38.0 38.0 36.0 38.0 26-27 37.21361343663876 38.0 38.0 38.0 36.0 38.0 28-29 37.161401729845906 38.0 38.0 38.0 36.0 38.0 30-31 37.14082297679094 38.0 38.0 38.0 36.0 38.0 32-33 37.08647929558179 38.0 38.0 38.0 36.0 38.0 34-35 37.13642469623698 38.0 38.0 38.0 36.0 38.0 36-37 37.14298095089525 38.0 38.0 38.0 36.0 38.0 38-39 36.62127554469346 38.0 37.5 38.0 34.0 38.0 40-41 36.86150399528539 38.0 37.5 38.0 35.5 38.0 42-43 37.053108696028985 38.0 38.0 38.0 36.0 38.0 44-45 37.1213058776628 38.0 38.0 38.0 36.0 38.0 46-47 37.0675948555284 38.0 38.0 38.0 36.0 38.0 48-49 37.09434420120292 38.0 38.0 38.0 36.0 38.0 50-51 36.96338810600939 38.0 38.0 38.0 35.5 38.0 52-53 36.98323453451892 38.0 38.0 38.0 36.0 38.0 54-55 36.92211360130345 38.0 38.0 38.0 35.5 38.0 56-57 36.986675159898084 38.0 38.0 38.0 36.0 38.0 58-59 36.88925866222939 38.0 37.5 38.0 35.5 38.0 60-61 36.9007071915137 38.0 38.0 38.0 35.0 38.0 62-63 36.859840015253155 38.0 37.5 38.0 35.0 38.0 64-65 36.79161683393133 38.0 37.5 38.0 34.5 38.0 66-67 36.52585235643839 38.0 37.5 38.0 34.0 38.0 68-69 36.440525713691436 38.0 37.0 38.0 34.0 38.0 70-71 35.923604250082334 38.0 37.0 38.0 32.5 38.0 72-73 35.08990258783561 38.0 37.0 38.0 25.5 38.0 74-75 34.83209401487182 38.0 37.0 38.0 24.0 38.0 76 34.16780198637616 38.0 35.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.017936903341706056 1101 2 -0.06442190790095026 1101 3 -0.03416999556592515 1101 4 0.10055964575644083 1101 5 -0.05178641603885126 1101 6 -0.039816981362932324 1101 7 -0.06344640606299379 1101 8 -0.0935322566902812 1101 9 -0.12895144103498524 1101 10-11 0.1446789935790207 1101 12-13 0.06800010258059785 1101 14-15 0.036934474526667316 1101 16-17 0.0984473324999442 1101 18-19 -0.04404353129669403 1101 20-21 -0.004640572272833765 1101 22-23 0.0398910028924675 1101 24-25 -0.08670315663317751 1101 26-27 -0.012091527783304912 1101 28-29 -0.196436651218022 1101 30-31 -0.008451056100717835 1101 32-33 -0.11639423092903911 1101 34-35 -0.060816875734190035 1101 36-37 0.09857489750347526 1101 38-39 0.07523393608386186 1101 40-41 0.1483217841760336 1101 42-43 0.012955068466240505 1101 44-45 0.08002348893570144 1101 46-47 -0.04157449149619197 1101 48-49 0.006302515877635528 1101 50-51 0.035463837721074754 1101 52-53 0.10314490828907452 1101 54-55 -0.10404858442971232 1101 56-57 -0.271201507161301 1101 58-59 -0.029854850802728095 1101 60-61 -0.027050980477163478 1101 62-63 -0.2752499328829643 1101 64-65 -0.4080610654778738 1101 66-67 -0.16007453037053665 1101 68-69 -0.6214778160569594 1101 70-71 -0.19040256886232498 1101 72-73 -0.4627868087675395 1101 74-75 -0.5245154519680284 1101 76 -1.3857307032286883 1102 1 -0.09148618681827969 1102 2 0.010988379521492675 1102 3 -0.06383655406813915 1102 4 0.04600543078451835 1102 5 -0.10976256086121339 1102 6 -0.16566140155231324 1102 7 0.06547578436543944 1102 8 0.14134192838892545 1102 9 -0.18349582287515886 1102 10-11 0.01428183338747857 1102 12-13 0.1597186390172638 1102 14-15 0.0034034952450738842 1102 16-17 0.02365653237800558 1102 18-19 0.2114359813732989 1102 20-21 0.2558440044600019 1102 22-23 0.17674853064648488 1102 24-25 0.0566393215126908 1102 26-27 -0.053371014887147794 1102 28-29 0.10765294827852046 1102 30-31 -0.024311897620130196 1102 32-33 0.31649464484900847 1102 34-35 0.1445382478191064 1102 36-37 0.15060199761458648 1102 38-39 -0.0420507429135526 1102 40-41 0.039872174059709664 1102 42-43 0.2127003903538025 1102 44-45 0.3149615893713147 1102 46-47 0.2209062109827471 1102 48-49 0.1508790520586487 1102 50-51 0.1344244756946651 1102 52-53 0.20337893682482644 1102 54-55 0.21390081427428242 1102 56-57 -0.06300952509693758 1102 58-59 0.31553881862704714 1102 60-61 0.21397382851425562 1102 62-63 0.3132335032065967 1102 64-65 0.22753782141160883 1102 66-67 0.5793868106719771 1102 68-69 0.4387353662394773 1102 70-71 0.011481459181759135 1102 72-73 -0.5125473720093296 1102 74-75 -0.2752750613960373 1102 76 -0.3861633610258721 1103 1 -0.03644031525864477 1103 2 -0.04099938806260184 1103 3 0.0890686447086182 1103 4 0.08881888644200586 1103 5 0.1471181730837401 1103 6 0.21048538743850997 1103 7 0.2367296069954108 1103 8 0.3095376470433635 1103 9 0.25075494165083967 1103 10-11 0.20541333185842348 1103 12-13 0.09091129956772193 1103 14-15 0.15936679799737163 1103 16-17 0.19643940699574358 1103 18-19 0.3184696205170283 1103 20-21 0.09376449375664464 1103 22-23 0.18898094654862518 1103 24-25 0.016883969830729484 1103 26-27 0.13776048358379 1103 28-29 0.2789067709084918 1103 30-31 0.28302755192115114 1103 32-33 0.15288608215787036 1103 34-35 0.09866668818607849 1103 36-37 0.07873655418951131 1103 38-39 -0.14449722609398208 1103 40-41 0.35791498751535755 1103 42-43 -0.07781948732203148 1103 44-45 0.20486984625204485 1103 46-47 0.08634963605919665 1103 48-49 0.08360076459687349 1103 50-51 0.1344244756946651 1103 52-53 0.09481624569333036 1103 54-55 0.19555219042107552 1103 56-57 0.050140321997865556 1103 58-59 0.3017773507371402 1103 60-61 0.16963132086899435 1103 62-63 0.20772891605063393 1103 64-65 0.29175800489785075 1103 66-67 0.02739904308787544 1103 68-69 0.28888827143824614 1103 70-71 0.01148145918176624 1103 72-73 0.25350767386223794 1103 74-75 0.07487784380274576 1103 76 0.49762868790379144 1104 1 0.00594267415009142 1104 2 -0.18286492116814657 1104 3 -0.005033070878745605 1104 4 0.07431755468704893 1104 5 0.052182429139413955 1104 6 -0.05274472367558758 1104 7 0.04849030363328666 1104 8 -0.08104769737447981 1104 9 0.04973617152878518 1104 10-11 0.02789084448095025 1104 12-13 0.09086645933347626 1104 14-15 -0.043149635948473986 1104 16-17 0.0996024371194153 1104 18-19 0.05845903822174847 1104 20-21 0.13288759813573847 1104 22-23 -0.05641171938265899 1104 24-25 0.2174005293292396 1104 26-27 0.10356980497169133 1104 28-29 0.0933578816010936 1104 30-31 -0.07912459996169474 1104 32-33 -0.06514058881345619 1104 34-35 0.05328388710633192 1104 36-37 0.1001118938542831 1104 38-39 0.16622317710940138 1104 40-41 0.14430956364062553 1104 42-43 0.04897075903021175 1104 44-45 -0.1297010775464642 1104 46-47 -0.2739909703601313 1104 48-49 0.0012247702646774883 1104 50-51 -0.4786850151893063 1104 52-53 -0.02070116576962988 1104 54-55 -0.08607138478048881 1104 56-57 -0.3002771405829563 1104 58-59 0.15953515966393184 1104 60-61 0.12204238026436798 1104 62-63 0.06328058545255288 1104 64-65 0.1781238832731944 1104 66-67 -0.14758997105472815 1104 68-69 -0.05555198791767424 1104 70-71 -0.09830537034463305 1104 72-73 0.2749054336441361 1104 74-75 0.0970109834262658 1104 76 0.34516292342264165 1105 1 -0.08259503266718582 1105 2 0.10100423635692124 1105 3 0.03467403603266206 1105 4 -0.048342740016252606 1105 5 0.08431377847506383 1105 6 -0.15034256589709116 1105 7 0.005814441064948994 1105 8 -0.10964912382537051 1105 9 -0.17194298563424582 1105 10-11 -0.04731914067527043 1105 12-13 -0.11190045714312191 1105 14-15 -0.12674997626924522 1105 16-17 -0.3976822375846183 1105 18-19 -0.12399271949882973 1105 20-21 0.0678555572972499 1105 22-23 -0.10598450820275929 1105 24-25 -0.09116903730483727 1105 26-27 -0.24000030472745237 1105 28-29 0.07317848910986413 1105 30-31 -0.030909473789577646 1105 32-33 0.08223821716750024 1105 34-35 0.07594327670119583 1105 36-37 0.1225269041725241 1105 38-39 0.2403425066050744 1105 40-41 0.07462979100386491 1105 42-43 -0.045567816691161056 1105 44-45 -0.07637661427575893 1105 46-47 -0.1728813074225144 1105 48-49 0.04673362222514754 1105 50-51 -0.3787565187554449 1105 52-53 -0.027720281659718182 1105 54-55 -0.05618922989832953 1105 56-57 -0.20416367618992837 1105 58-59 -0.09471432442425964 1105 60-61 0.2117793557540395 1105 62-63 -0.03901476953097216 1105 64-65 -0.3111415307233969 1105 66-67 -0.7116852246661125 1105 68-69 0.07170625762014993 1105 70-71 -0.06208293089638772 1105 72-73 0.3053397046697981 1105 74-75 0.47751404269276776 1105 76 -0.021116356835136685 1106 1 0.0631409597354704 1106 2 0.003121995141349032 1106 3 -0.0414418234165268 1106 4 0.0848386464984685 1106 5 0.001787662615612362 1106 6 0.027083834862644096 1106 7 0.035054706971884286 1106 8 0.08324736240444963 1106 9 0.2518746781834196 1106 10-11 0.16872549370269496 1106 12-13 0.09047846023580064 1106 14-15 0.1574143162283761 1106 16-17 0.13491035500797466 1106 18-19 0.04645362428085065 1106 20-21 -0.016275496833806358 1106 22-23 0.08576288021129841 1106 24-25 0.08968566354985796 1106 26-27 0.06002818530103582 1106 28-29 0.38627915387719725 1106 30-31 0.20104684150007301 1106 32-33 0.060291398554021214 1106 34-35 0.15382076957869373 1106 36-37 0.24014798679360183 1106 38-39 0.10101677061267367 1106 40-41 0.10092133896208111 1106 42-43 -0.0794614539181282 1106 44-45 0.10820553197304861 1106 46-47 0.26585563464776385 1106 48-49 0.053970089461614634 1106 50-51 0.20874110707551807 1106 52-53 0.10465863571920409 1106 54-55 0.13644609773700012 1106 56-57 0.21804904935613223 1106 58-59 -0.04481403952397045 1106 60-61 0.15918201482336514 1106 62-63 0.05707259988972879 1106 64-65 -0.08791266082786109 1106 66-67 0.1997820118011191 1106 68-69 0.26557140481862973 1106 70-71 -0.07074389955735683 1106 72-73 -0.2002490892523241 1106 74-75 -0.2539342004221439 1106 76 0.08004223765679086 1107 1 0.1901390835628618 1107 2 -0.17413879285114575 1107 3 0.004336788215745457 1107 4 0.12280299921891924 1107 5 0.11698466073944047 1107 6 0.0711645102847811 1107 7 0.04243744618388945 1107 8 0.23719670574404006 1107 9 0.10233434047234624 1107 10-11 0.11230898338374828 1107 12-13 0.005759886126980973 1107 14-15 0.14737679277622107 1107 16-17 0.2284469403738285 1107 18-19 0.005825106457209017 1107 20-21 0.07892150129001863 1107 22-23 0.01831916538953493 1107 24-25 0.04179166730220629 1107 26-27 -5.58118638920746E-4 1107 28-29 0.030589660443808953 1107 30-31 0.05611250754135 1107 32-33 0.05165631412624805 1107 34-35 0.05990894969126259 1107 36-37 0.04602509748778516 1107 38-39 0.08447830907421405 1107 40-41 -0.07573907379589429 1107 42-43 0.25607325527511193 1107 44-45 0.16282091658843711 1107 46-47 0.15138096297796722 1107 48-49 0.15620742529650755 1107 50-51 0.15095968118433944 1107 52-53 0.06250103722013733 1107 54-55 -0.12348823622172489 1107 56-57 0.08509407749872366 1107 58-59 0.2311099754854098 1107 60-61 -0.34729547110534753 1107 62-63 0.07962419463644466 1107 64-65 0.34494850239915564 1107 66-67 0.3581544320637846 1107 68-69 0.21964738980924636 1107 70-71 0.12919981526439983 1107 72-73 0.004225582417468843 1107 74-75 -0.10596984770169371 1107 76 -0.5439633908610304 1108 1 -0.010934815235827955 1108 2 0.07435046816086555 1108 3 0.18955702847784295 1108 4 -0.02110854064275003 1108 5 0.006609706243629887 1108 6 0.035625740717982524 1108 7 0.07905011454938204 1108 8 -0.02426124838773802 1108 9 -0.21627687175917742 1108 10-11 -0.14624580365665452 1108 12-13 -0.1506926075024353 1108 14-15 0.14007792128004581 1108 16-17 0.029238713216926726 1108 18-19 -0.07519964782018462 1108 20-21 0.05212863634644549 1108 22-23 0.10743911442484233 1108 24-25 0.14183262106995187 1108 26-27 0.040900745576578856 1108 28-29 -0.02880695404472533 1108 30-31 0.15041538340592808 1108 32-33 -0.09973156355849966 1108 34-35 -0.22282676199421303 1108 36-37 0.0986324873676594 1108 38-39 -0.16440228794071032 1108 40-41 0.07460675802063577 1108 42-43 -0.02667155724763859 1108 44-45 -0.008074605799620826 1108 46-47 0.0016994922826683023 1108 48-49 0.13589890691281425 1108 50-51 0.1579718763062914 1108 52-53 0.08199503066753522 1108 54-55 -0.09000625587953692 1108 56-57 -0.19398309760138233 1108 58-59 0.03481856667579564 1108 60-61 -0.09728181984942808 1108 62-63 0.10729073995861427 1108 64-65 0.03525611069911605 1108 66-67 -0.0728930743068048 1108 68-69 -0.22809105212741088 1108 70-71 -0.05749476063657255 1108 72-73 -0.3886302603200633 1108 74-75 -0.6297321338320145 1108 76 0.055426853041410595 1109 1 0.14704592327345978 1109 2 0.1302544345673624 1109 3 0.17469555602360032 1109 4 0.07352836656433226 1109 5 0.06760746971982456 1109 6 -0.0341629306043032 1109 7 0.18168373543577587 1109 8 0.06488932900055033 1109 9 0.05503628721658771 1109 10-11 -0.14779767731589288 1109 12-13 0.13984096317628314 1109 14-15 0.13337291420532438 1109 16-17 -0.04362175508376254 1109 18-19 0.05547267862100114 1109 20-21 0.1625718332061865 1109 22-23 -0.03426064366544068 1109 24-25 0.13615002487659922 1109 26-27 0.26925895453180715 1109 28-29 0.005206465098090973 1109 30-31 0.007798194122983659 1109 32-33 0.0932530546349355 1109 34-35 0.12771867595366615 1109 36-37 0.10320138599379192 1109 38-39 0.24999818675005514 1109 40-41 0.40072844317285217 1109 42-43 0.13930589494096068 1109 44-45 0.12383009090036268 1109 46-47 0.3478175259674501 1109 48-49 0.23956406734916413 1109 50-51 0.23228380291179462 1109 52-53 0.4862535545618272 1109 54-55 0.1405063188614335 1109 56-57 0.3330149397348734 1109 58-59 0.16722077581358974 1109 60-61 0.27819401200049754 1109 62-63 -0.07514570168637391 1109 64-65 0.024173907038523623 1109 66-67 0.3530871164823708 1109 68-69 0.1604479044657765 1109 70-71 0.5925212145334484 1109 72-73 0.06849238334235963 1109 74-75 0.3057646939556449 1109 76 0.16123725059492244 1110 1 -0.09339750180298978 1110 2 -0.17009915870480796 1110 3 0.006394715045011878 1110 4 0.03873287726769803 1110 5 -0.049173875845923476 1110 6 -0.04613731898351148 1110 7 -0.15984913918196497 1110 8 0.08483390086598774 1110 9 0.018797755106497505 1110 10-11 -0.2625004678357641 1110 12-13 0.03312269100503329 1110 14-15 -0.010854914540850302 1110 16-17 0.14491035500797977 1110 18-19 0.16013150889622807 1110 20-21 0.14870046470466036 1110 22-23 -0.13183327363486086 1110 24-25 -0.07120375952706581 1110 26-27 -0.22917854546819427 1110 28-29 0.12620703849258774 1110 30-31 0.1142679953462249 1110 32-33 0.06670966778478515 1110 34-35 -0.08105903811360804 1110 36-37 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0.10547618260837766 2115 64-65 0.013926116832827518 2115 66-67 -0.11325803034851134 2115 68-69 -0.05739487547641886 2115 70-71 0.1573488296312604 2115 72-73 0.5232282553209089 2115 74-75 0.4828624276073583 2115 76 0.23556383934278102 2116 1 0.07947483862254501 2116 2 -0.047603591328922334 2116 3 -0.011490923252864604 2116 4 -0.001980775214569519 2116 5 -0.013269620526777715 2116 6 -9.467161466290008E-4 2116 7 0.10464554166910034 2116 8 0.1632691845539327 2116 9 -0.016308627872227532 2116 10-11 0.020023911596865673 2116 12-13 0.10982925128871557 2116 14-15 0.11494295779957753 2116 16-17 -0.2589903542118819 2116 18-19 -0.1207749627349699 2116 20-21 0.023135305130189465 2116 22-23 0.07007275473393548 2116 24-25 -0.1892777843497555 2116 26-27 0.2533015077232932 2116 28-29 0.06316404203868586 2116 30-31 0.009148314495163845 2116 32-33 -0.015016839307413932 2116 34-35 0.12371711436865951 2116 36-37 -0.10629500284087356 2116 38-39 0.42579036581180674 2116 40-41 -0.08983698073241442 2116 42-43 -0.029085021839570402 2116 44-45 -0.06058333873071575 2116 46-47 0.11520642570067707 2116 48-49 0.3489400840060739 2116 50-51 0.39130520963961857 2116 52-53 0.08762644804871655 2116 54-55 0.15844827995194777 2116 56-57 -0.16587893755058047 2116 58-59 -0.029604001335208352 2116 60-61 -0.09334935451651916 2116 62-63 0.3276781645378435 2116 64-65 -0.15360278630522117 2116 66-67 -0.20646457848802413 2116 68-69 -0.3086457255687165 2116 70-71 -0.08257150457644968 2116 72-73 0.18579019061674273 2116 74-75 0.30293432112722485 2116 76 -0.06655896256143023 2117 1 -0.12784632070062685 2117 2 0.09242544759440818 2117 3 -0.07699603298648583 2117 4 -0.10691180777167375 2117 5 -0.05557151364119761 2117 6 -0.09699853945595294 2117 7 0.07582999467630458 2117 8 -0.47340920937023157 2117 9 0.04123869998838359 2117 10-11 -0.16942714106411216 2117 12-13 -0.27182317513670284 2117 14-15 0.008141148451272784 2117 16-17 0.11420169359065113 2117 18-19 0.08602963881749304 2117 20-21 -0.18615435812998982 2117 22-23 -0.05973681694194255 2117 24-25 -0.3921314170073771 2117 26-27 -0.14215836830283735 2117 28-29 -0.6572944378853691 2117 30-31 -0.08408338260652926 2117 32-33 -0.35362743851442957 2117 34-35 -0.1630841365388136 2117 36-37 0.23126851980389773 2117 38-39 0.19373027757815464 2117 40-41 0.13119390104566264 2117 42-43 0.08663787981899418 2117 44-45 -0.0812735716005406 2117 46-47 -0.0794381267089932 2117 48-49 -0.42031216371827895 2117 50-51 -0.07391788747323602 2117 52-53 -0.3785878815430621 2117 54-55 0.07194579489024022 2117 56-57 -0.20353181072866278 2117 58-59 0.14453181147541727 2117 60-61 -0.03786825773872238 2117 62-63 0.14016769377222005 2117 64-65 0.013831736507086134 2117 66-67 0.2257965484455724 2117 68-69 0.2196113805908837 2117 70-71 0.1211337503517882 2117 72-73 0.42008404218316997 2117 74-75 0.9595909358588912 2117 76 0.2648756719390448 2118 1 -0.13540155038598556 2118 2 -0.11153387530399783 2118 3 -0.3224134833355592 2118 4 -0.05993868143675485 2118 5 -0.007928936574671752 2118 6 -0.18716717728310073 2118 7 -0.3615444833115191 2118 8 -0.21586194933643554 2118 9 -0.23687026108783726 2118 10-11 -0.12026236054831685 2118 12-13 -0.3367280174969949 2118 14-15 -0.10331442871089536 2118 16-17 -0.325332559971784 2118 18-19 -0.28358686154911084 2118 20-21 -0.3212957397487912 2118 22-23 0.023657617053395086 2118 24-25 -0.18788518665257214 2118 26-27 -0.2671782924317512 2118 28-29 -0.11444959106206909 2118 30-31 0.1018565580992643 2118 32-33 -0.4229474678427394 2118 34-35 -0.4944383397330512 2118 36-37 -0.23361719539263248 2118 38-39 -0.2125864682537184 2118 40-41 -0.4879045719690964 2118 42-43 -0.28367198023391893 2118 44-45 -0.3867742251119708 2118 46-47 -0.31414436535224155 2118 48-49 -0.07712302794729453 2118 50-51 -0.25579330587994065 2118 52-53 -0.14019966387593996 2118 54-55 -0.19262272817393722 2118 56-57 -0.22186997898395333 2118 58-59 -0.46821484924056733 2118 60-61 -0.3864050297110495 2118 62-63 -0.3409840802722144 2118 64-65 -0.2172244017185534 2118 66-67 0.13055124257034834 2118 68-69 -0.6731330486226668 2118 70-71 -0.4740637376140384 2118 72-73 -0.46850819856405934 2118 74-75 -0.239116386656967 2118 76 -1.0919415680112223 2119 1 0.013309815270183378 2119 2 0.037243320969992055 2119 3 0.004336788215745457 2119 4 -0.06520513086238822 2119 5 -0.1187876969841355 2119 6 0.17177426638234294 2119 7 -0.21874141560472538 2119 8 -0.3247951641746596 2119 9 -0.27266565952765376 2119 10-11 -0.2149267889739761 2119 12-13 -0.11720759354781762 2119 14-15 -0.6656313373050793 2119 16-17 -0.2664717588131609 2119 18-19 -0.029743999233843965 2119 20-21 -0.08774516537664567 2119 22-23 0.08590046620253844 2119 24-25 0.04026727705830524 2119 26-27 0.09649472688953153 2119 28-29 -0.04512172167001438 2119 30-31 -0.3178712322960493 2119 32-33 -0.6012908403452926 2119 34-35 -0.1829772291705254 2119 36-37 -0.020539943162624752 2119 38-39 -0.06897697547863402 2119 40-41 0.31653734896833186 2119 42-43 -0.0914877203346407 2119 44-45 -0.2558782704034357 2119 46-47 -0.2337816386480469 2119 48-49 -0.10751208689860903 2119 50-51 0.0681344779323112 2119 52-53 0.05437095591933172 2119 54-55 -0.18751262646562594 2119 56-57 -0.3142961664037145 2119 58-59 -0.05801604077475275 2119 60-61 0.17252160206538747 2119 62-63 -0.1500506021115271 2119 64-65 -0.0910271073569433 2119 66-67 -0.07426426712321188 2119 68-69 -0.23309651262977837 2119 70-71 0.332451847784732 2119 72-73 0.7064613547751932 2119 74-75 0.4722821848186314 2119 76 -0.3437601388285074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 2.0 22 6.0 23 9.0 24 25.0 25 25.0 26 96.0 27 156.0 28 296.0 29 514.0 30 845.0 31 1372.0 32 2172.0 33 3657.0 34 6057.0 35 9996.0 36 15412.0 37 74745.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.5611252664968 16.28533790927842 11.412129721110013 32.74140710311476 2 21.79813842233893 19.4555665332016 33.01007054582012 25.73622449863935 3 19.104570745151058 18.919972960324476 31.18662576049087 30.78883053403359 4 14.415093685542441 21.223545317456193 33.78659456086527 30.5747664361361 5 14.623091189572394 31.40155651465516 37.95261123533184 16.022741060440605 6 29.0615845943182 37.39708456831851 17.21959336487962 16.32173747248366 7 28.1983949525939 26.667879985440173 21.303277694001004 23.83044736796492 8 24.6243045083459 35.90816910197078 16.853864420293625 22.6136619693897 9 23.02619035238244 16.80359835681972 18.08278300660392 42.087428284193926 10-11 25.747924358241036 25.967188393739278 24.634271055414 23.65061619260569 12-13 25.019066437869412 20.408021770405423 25.93858873693516 28.634323054790013 14-15 22.16170072625795 20.008493231414555 21.736605827396737 36.093200214930754 16-17 26.036954223215986 26.085920302289704 21.42460957135181 26.452515903142498 18-19 27.32913871700206 24.92503423292254 22.24403307160314 25.50179397847226 20-21 23.837813946232643 23.612483316866864 21.892170627285807 30.65753210961468 22-23 22.96205778863987 26.69864628291127 22.075035099578805 28.264260828870054 24-25 26.00618792574489 26.74241242438424 22.758826894077274 24.4925727557936 26-27 27.375071499142013 27.266739465793076 22.61279531312291 22.745393721942005 28-29 23.002790633179067 27.65240150451528 23.87724680637166 25.467561055933995 30-31 22.87669214636091 28.132095748184355 25.431161492728755 23.56005061272598 32-33 27.806666320004158 23.736848491151438 25.227930598166154 23.228554590678247 34-35 25.098798814414224 24.70317022862392 27.332172013935836 22.86585894302602 36-37 26.557381311424262 26.165219350701125 24.583138335673304 22.69426100220131 38-39 22.51356317057529 25.03943286013901 27.30400568526511 25.14299828402059 40-41 21.985336175965887 21.62004055951329 28.38342606555388 28.011197198966947 42-43 25.116565267883452 21.069713830100707 27.82659941414036 25.98712148787548 44-45 21.294177803199695 24.567971851004454 30.215104085417643 23.922746260378208 46-47 23.244587731613887 21.934203456225195 29.030384968713708 25.790823843447214 48-49 22.711594127537136 21.15681278491325 27.252439637391017 28.879153450158597 50-51 21.331877350805122 22.08673495918049 29.003951952576568 27.577435737437817 52-53 18.350146464909088 22.148267554122683 34.033591596900834 25.467994384067396 54-55 18.86277364671624 22.809092957551176 30.635865702944898 27.692267692787688 56-57 21.0701471582341 23.603383426065555 26.987676147886226 28.338793267814122 58-59 19.036104900074534 23.445651985509507 30.68439845388522 26.833844660530744 60-61 21.39947653961486 25.92688887733347 27.334771982736207 25.338862600315466 62-63 18.488811467595724 31.76425216230739 23.858180368502243 25.888756001594647 64-65 18.45241190439048 33.289567191860364 22.375764824155446 25.882256079593713 66-67 18.53691089040265 32.655608132702405 21.642573622449866 27.164907354445077 68-69 18.22101468115716 32.95937115421282 21.930736831158026 26.888877333472 70-71 18.519144436933423 32.207113514637825 22.287365884942716 26.986376163486035 72-73 19.121037214220095 30.539666857331042 22.45419721630007 27.885098712148785 74-75 19.87416151006188 30.33816927530203 22.642261626193818 27.14540758844227 76 19.985093512211186 30.92489556791985 22.260932868805575 26.829078051063387 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 1.0 18 3.0 19 7.5 20 11.5 21 13.0 22 17.5 23 35.0 24 59.5 25 71.0 26 140.0 27 216.0 28 292.5 29 362.0 30 470.5 31 655.0 32 921.0 33 1111.0 34 1271.5 35 1657.0 36 2075.5 37 2269.0 38 2716.5 39 4862.0 40 7571.5 41 8437.0 42 8291.0 43 7708.5 44 7054.5 45 6753.5 46 6524.0 47 6551.5 48 6420.5 49 5944.0 50 5626.0 51 5310.5 52 4437.0 53 3572.5 54 3266.0 55 3062.5 56 2626.0 57 2231.0 58 2069.0 59 2052.0 60 1973.0 61 1932.0 62 1953.0 63 1956.0 64 1957.0 65 1975.5 66 1934.0 67 1872.0 68 1845.5 69 1736.5 70 1526.5 71 1399.0 72 1245.0 73 938.5 74 621.0 75 456.0 76 350.0 77 165.5 78 59.0 79 31.0 80 17.5 81 3.5 82 2.5 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 115386.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.263844833861995 #Duplication Level Percentage of deduplicated Percentage of total 1 64.86081450676258 30.00710658138769 2 18.67670750440223 17.281125959821818 3 6.622082349855757 9.190889709323487 4 3.0328575175152674 5.6124659837415285 5 1.7440335693679518 4.034284921914271 6 1.1445805702296654 3.177161874057511 7 0.7661758645236222 2.481236891823965 8 0.6387921022067364 2.3642382958071173 9 0.4627027837098648 1.9265768810774269 >10 2.0137874189801805 13.915033019603765 >50 0.022479487467685737 0.6647253566290537 >100 0.007493162489228579 0.7635241710432809 >500 0.0 0.0 >1k 0.005619871866921434 3.8999532005615936 >5k 0.0018732906223071447 4.681677153207494 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 5402 4.681677153207494 No Hit CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG 1666 1.443849340474581 RNA PCR Primer, Index 23 (95% over 24bp) TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 1493 1.2939178063196575 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 1341 1.1621860537673547 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 376 0.3258627563135909 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 243 0.210597472830326 RNA PCR Primer, Index 47 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT 140 0.12133187735080511 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT 122 0.10573206454855875 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0025999688003743955 0.0 2 0.0 0.0 0.0 0.004333281333957326 0.0 3 0.0 0.0 0.0 0.004333281333957326 0.0 4 0.0 0.0 0.0 0.011266531468289048 0.0 5 0.0 0.0 0.0 0.012133187735080512 0.0 6 0.0 0.0 0.0 0.012133187735080512 0.0 7 0.0 0.0 0.0 0.012999844001871978 0.0 8 0.0 0.0 0.0 0.013866500268663443 0.0 9 0.0 0.0 0.0 0.01646646906903784 0.0 10 0.0 0.0 0.0 0.017333125335829303 0.0 11 0.0 0.0 0.0 0.019066437869412235 0.0 12 0.0 0.0 0.0 0.0199330941362037 0.0 13 0.0 0.0 0.0 0.024266375470161024 0.0 14 0.0 0.0 0.0 0.02513303173695249 0.0 15 0.0 0.0 0.0 0.025999688003743956 0.0 16 0.0 0.0 0.0 0.02686634427053542 0.0 17 0.0 0.0 0.0 0.03379959440486714 0.0 18 0.0 0.0 0.0 0.03379959440486714 0.0 19 0.0 0.0 0.0 0.04073284453919886 0.0 20 0.0 0.0 0.0 0.04679943840673912 0.0 21 0.0 0.0 0.0 0.05026606347390498 0.0 22 0.0 0.0 0.0 0.05546600107465377 0.0 23 0.0 0.0 0.0 0.06413256374256843 0.0 24 0.0 0.0 0.0 0.07193247014369161 0.0 25 0.0 0.0 0.0 0.0797323765448148 0.0 26 0.0 0.0 0.0 0.09099890801310384 0.0 27 0.0 0.0 0.0 0.17766453469225035 0.0 28 0.0 0.0 0.0 0.25479694243669077 0.0 29 0.0 0.0 0.0 0.3631289757856239 0.0 30 0.0 0.0 0.0 0.46886104033418263 0.0 31 0.0 0.0 0.0 0.5936595427521536 0.0 32 0.0 0.0 0.0 0.7427244206402857 0.0 33 0.0 0.0 0.0 0.8943892673287921 0.0 34 0.0 0.0 0.0 1.1335863969632365 0.0 35 0.0 0.0 0.0 1.4256495588719602 0.0 36 0.0 0.0 0.0 1.8624443173348586 0.0 37 0.0 0.0 0.0 2.4916367670254624 0.0 38 0.0 0.0 0.0 3.2837605948728616 0.0 39 0.0 0.0 0.0 4.0750177664534695 0.0 40 0.0 0.0 0.0 4.985873502851299 0.0 41 0.0 0.0 0.0 6.107326712079455 0.0 42 0.0 0.0 0.0 7.430710831470022 0.0 43 0.0 0.0 0.0 8.829494046071447 0.0 44 0.0 0.0 0.0 10.057545976114953 0.0 45 0.0 0.0 0.0 11.18506577921065 0.0 46 0.0 0.0 0.0 12.290919175636558 0.0 47 0.0 0.0 0.0 13.341306570987815 0.0 48 0.0 0.0 0.0 14.312828246061047 0.0 49 0.0 0.0 0.0 15.176884544052138 0.0 50 0.0 0.0 0.0 16.009741216438737 0.0 51 0.0 0.0 0.0 16.93013017177127 0.0 52 0.0 0.0 0.0 18.044650130865097 0.0 53 0.0 0.0 0.0 19.333367999584006 0.0 54 0.0 0.0 0.0 20.600419461633127 0.0 55 0.0 0.0 0.0 21.576274418040317 0.0 56 0.0 0.0 0.0 22.50706324857435 0.0 57 0.0 0.0 0.0 23.37631948416619 0.0 58 0.0 0.0 0.0 24.107777373338187 0.0 59 0.0 0.0 0.0 24.833168668642642 0.0 60 0.0 0.0 0.0 25.5888929332848 0.0 61 0.0 0.0 0.0 26.317750853656424 0.0 62 0.0 0.0 0.0 27.10900802523703 0.0 63 0.0 0.0 0.0 28.009463886433362 0.0 64 0.0 0.0 0.0 28.906453122562528 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGTAT 15 0.00221982 70.0 39 TGCGGGT 20 0.006936271 52.5 45 CTCGGGC 20 0.006936271 52.5 2 TAGCTTG 20 0.006936271 52.5 23 CGATATG 20 0.006936271 52.5 24 CTTTATA 50 6.136543E-8 48.999996 2 TTAATAC 45 1.6725389E-6 46.666664 3 TCTTTAT 55 1.298904E-7 44.545452 1 TTTATAC 80 2.910383E-11 43.75 3 CTTAATA 50 3.4536351E-6 42.0 2 CGCTCTA 35 0.0012510786 40.000004 14 AAAGACC 35 0.0012510786 40.000004 69 TAATACA 75 3.4950062E-8 37.333336 4 TATGCCG 1420 0.0 35.24648 43 TCTGACT 40 0.0024104516 35.0 59 AACGGCC 40 0.0024104516 35.0 24 ACTACAC 50 1.7009799E-4 35.0 3 ATGCCGT 1420 0.0 35.0 44 GCCGTCT 1420 0.0 35.0 46 TCGTATG 1425 0.0 34.87719 40 >>END_MODULE