FastQCFastQC Report
Wed 25 May 2016
SRR1780229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716967
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTGC207.828991E-444.0290639
GGCTAGC300.005766133329.30970844
AAACGTA609.643485E-625.70157215
TTAAACG450.001391724824.462314
AAAACGT651.8018412E-523.70961214
CGAAACT703.217406E-521.9960925
CGATTTC703.2295626E-521.9853542
ACGGTAC500.002595718721.976158
TTCGGTA856.117105E-620.70220426
GAAAACG755.4409116E-520.54689413
GAAACTC755.476133E-520.52968426
ATGCCAT755.502679E-520.51679443
GTTAATA654.9656583E-420.2856772
GCTAGTC809.057195E-519.229137
ATTAGAT708.1057526E-418.8616924
TCGGTAA1051.7936563E-618.85379227
CTAAATT708.143891E-418.8485310
CGGTAAG951.5980739E-518.52302428
TCAAATG600.007381061618.34544240
AAACTCG600.007416827618.33007627
Produced by FastQC (version 0.11.5)