FastQCFastQC Report
Wed 25 May 2016
SRR1780229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716967
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT214722.994837977201182No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG58240.8123107479144785No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC58070.8099396485472832No Hit
CACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTG37370.5212234314828995Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC12670.17671664107274115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA301.3010195E-436.66469240
GTTGCGT250.002352778335.19810538
TCTTCTG4900.032.77374344
CTTTATA1350.032.5953832
ATGCCGT29850.031.98487744
GTCTTCT5250.030.58882743
CGTCTTC5300.030.30025542
CCGTCTT5350.030.01707541
GATCATA406.951649E-427.5523191
TATGCCG34600.027.5303143
ATGCCAA407.031537E-427.49851810
ATACCAT609.749834E-625.6652836
CGGCTTA450.001398098124.44312915
TCTTTAT1850.023.8290331
CTATAAC651.8108562E-523.6943364
TTCCATA42500.022.82700529
CTTCCAT42750.022.69351428
CGAGACT42850.022.64055323
GAGACTT43000.022.56157724
CGTATGC42200.022.46798341
Produced by FastQC (version 0.11.5)