FastQCFastQC Report
Wed 25 May 2016
SRR1780228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128420
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCAA252.3830187E-456.00000413
ACACGTG200.006938052452.58
CGCTGGT200.006938052452.527
CGCTGCA200.006938052452.516
ATGTACC200.006938052452.554
TGCAAAG352.0770318E-550.00000415
GGCTATA404.5719295E-543.7530
AGAGTAG350.001251607840.00000427
AGTGTGC350.001251607840.0000047
TAGAGTA400.002411465935.026
ACGGGTT400.002411465935.037
ATGACGG400.002411465935.034
TGCAGAC400.002411465935.019
GTTGGGC400.002411465935.041
CGGGTTG450.00429435931.11110938
GCAAAGA450.00429435931.11110916
TAATTGG450.00429435931.11110934
GTAGAGT450.00429435931.11110925
TACTGCG450.00429435931.11110932
TAGATGA450.00429435931.11110931