Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780228_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 128420 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT | 7664 | 5.967917769817785 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC | 2332 | 1.8159165239059336 | No Hit |
CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG | 2013 | 1.567512848465971 | Illumina PCR Primer Index 9 (96% over 25bp) |
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG | 1965 | 1.5301354929138764 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC | 485 | 0.3776670300576234 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT | 235 | 0.18299330322379692 | No Hit |
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT | 193 | 0.15028811711571408 | Illumina PCR Primer Index 9 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT | 140 | 0.10901728702694286 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCTTGAT | 20 | 0.0069380524 | 52.5 | 37 |
GCTTATA | 20 | 0.0069380524 | 52.5 | 3 |
GTCAATT | 20 | 0.0069380524 | 52.5 | 12 |
AAGCTGG | 20 | 0.0069380524 | 52.5 | 70 |
CTTTATA | 35 | 2.0770318E-5 | 50.000004 | 2 |
CAGCAAA | 30 | 5.8598054E-4 | 46.666664 | 20 |
TATACCG | 30 | 5.8598054E-4 | 46.666664 | 6 |
TAAGCGC | 30 | 5.8598054E-4 | 46.666664 | 47 |
TTCAGCA | 35 | 0.0012516078 | 40.000004 | 18 |
GTAAGCG | 35 | 0.0012516078 | 40.000004 | 46 |
CCCAGGG | 50 | 1.7020512E-4 | 35.000004 | 2 |
TCTTTAT | 50 | 1.7020512E-4 | 35.000004 | 1 |
TCTTCAG | 40 | 0.0024114659 | 35.0 | 16 |
CCATCGT | 40 | 0.0024114659 | 35.0 | 31 |
TCAGCAA | 40 | 0.0024114659 | 35.0 | 19 |
TATGCCG | 1725 | 0.0 | 34.492752 | 43 |
CGTATGC | 1725 | 0.0 | 34.289852 | 41 |
GTATGCC | 1725 | 0.0 | 34.289852 | 42 |
TCGTATG | 1725 | 0.0 | 34.289852 | 40 |
CTCGTAT | 1715 | 0.0 | 34.285713 | 39 |