FastQCFastQC Report
Wed 25 May 2016
SRR1780228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128420
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT76645.967917769817785No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC23321.8159165239059336No Hit
CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG20131.567512848465971Illumina PCR Primer Index 9 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG19651.5301354929138764No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4850.3776670300576234No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT2350.18299330322379692No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT1930.15028811711571408Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1400.10901728702694286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAT200.006938052452.537
GCTTATA200.006938052452.53
GTCAATT200.006938052452.512
AAGCTGG200.006938052452.570
CTTTATA352.0770318E-550.0000042
CAGCAAA305.8598054E-446.66666420
TATACCG305.8598054E-446.6666646
TAAGCGC305.8598054E-446.66666447
TTCAGCA350.001251607840.00000418
GTAAGCG350.001251607840.00000446
CCCAGGG501.7020512E-435.0000042
TCTTTAT501.7020512E-435.0000041
TCTTCAG400.002411465935.016
CCATCGT400.002411465935.031
TCAGCAA400.002411465935.019
TATGCCG17250.034.49275243
CGTATGC17250.034.28985241
GTATGCC17250.034.28985242
TCGTATG17250.034.28985240
CTCGTAT17150.034.28571339