##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780228_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 128420 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.7408814826351 34.0 34.0 34.0 34.0 34.0 2 33.73230805170534 34.0 34.0 34.0 34.0 34.0 3 33.762723874785856 34.0 34.0 34.0 34.0 34.0 4 33.795195452421744 34.0 34.0 34.0 34.0 34.0 5 33.79292166329232 34.0 34.0 34.0 34.0 34.0 6 37.56799563930852 38.0 38.0 38.0 37.0 38.0 7 37.57815760784924 38.0 38.0 38.0 37.0 38.0 8 37.481615013237814 38.0 38.0 38.0 37.0 38.0 9 37.4925478897368 38.0 38.0 38.0 37.0 38.0 10-11 37.454037533094535 38.0 38.0 38.0 37.0 38.0 12-13 37.44135259305404 38.0 38.0 38.0 37.0 38.0 14-15 37.373294658152936 38.0 38.0 38.0 37.0 38.0 16-17 37.394506307428756 38.0 38.0 38.0 37.0 38.0 18-19 37.37107927114157 38.0 38.0 38.0 36.5 38.0 20-21 37.3909866064476 38.0 38.0 38.0 37.0 38.0 22-23 37.380322379691634 38.0 38.0 38.0 37.0 38.0 24-25 37.38678944089706 38.0 38.0 38.0 37.0 38.0 26-27 37.29456081607226 38.0 38.0 38.0 36.5 38.0 28-29 37.27995249961065 38.0 38.0 38.0 36.0 38.0 30-31 37.27230182214608 38.0 38.0 38.0 36.0 38.0 32-33 37.20892384363806 38.0 38.0 38.0 36.0 38.0 34-35 37.26286793334371 38.0 38.0 38.0 36.5 38.0 36-37 37.26037610964025 38.0 38.0 38.0 36.5 38.0 38-39 36.65817629652702 38.0 38.0 38.0 34.0 38.0 40-41 36.92431085500701 38.0 38.0 38.0 35.5 38.0 42-43 37.1762848465971 38.0 38.0 38.0 36.0 38.0 44-45 37.24326818252609 38.0 38.0 38.0 36.0 38.0 46-47 37.20018688677776 38.0 38.0 38.0 36.0 38.0 48-49 37.21470175985049 38.0 38.0 38.0 36.0 38.0 50-51 37.09481389191714 38.0 38.0 38.0 36.0 38.0 52-53 37.104563152156985 38.0 38.0 38.0 36.0 38.0 54-55 37.071484192493386 38.0 38.0 38.0 36.0 38.0 56-57 37.11431241239683 38.0 38.0 38.0 36.0 38.0 58-59 37.01570627628095 38.0 38.0 38.0 36.0 38.0 60-61 37.03318797695063 38.0 38.0 38.0 36.0 38.0 62-63 37.013522037065876 38.0 38.0 38.0 36.0 38.0 64-65 36.93261952966827 38.0 38.0 38.0 35.5 38.0 66-67 36.6399470487463 38.0 37.5 38.0 34.5 38.0 68-69 36.54896433577324 38.0 38.0 38.0 34.0 38.0 70-71 35.91944401183616 38.0 37.0 38.0 31.5 38.0 72-73 34.85903286092509 38.0 37.0 38.0 24.0 38.0 74-75 34.556264600529516 38.0 37.0 38.0 23.0 38.0 76 33.986653169288275 38.0 35.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.11885344611853554 1101 2 -0.07844984010333178 1101 3 -0.0459744670232638 1101 4 -0.10059113079742588 1101 5 -0.16922487787762464 1101 6 -0.09558029335398999 1101 7 -0.027358992276980132 1101 8 -0.007298772649086516 1101 9 -0.0869423821184725 1101 10-11 -0.1797065362790704 1101 12-13 -0.11217348598793819 1101 14-15 0.1185401550885743 1101 16-17 -0.048913881002633275 1101 18-19 -0.16799305144596133 1101 20-21 -0.11450937018476282 1101 22-23 -0.14907148858362973 1101 24-25 -0.2209247776876353 1101 26-27 -0.2599328448916651 1101 28-29 -0.38800310341922284 1101 30-31 -0.4365039631524894 1101 32-33 -0.30663521262402327 1101 34-35 -0.19256018169602385 1101 36-37 -0.2129373280912361 1101 38-39 -0.3220282632415774 1101 40-41 -0.37835297435628945 1101 42-43 0.0831789346072398 1101 44-45 -0.21313761315079915 1101 46-47 -0.17978311742460562 1101 48-49 -0.08574323264378592 1101 50-51 -0.323289522633992 1101 52-53 -0.2970060199320912 1101 54-55 -0.17944434206504667 1101 56-57 -0.25646698442754 1101 58-59 -0.4559514420760422 1101 60-61 -0.3863957787280654 1101 62-63 -0.1385679830561557 1101 64-65 -0.4047385542079951 1101 66-67 -0.75524994105632 1101 68-69 -0.8805087106586527 1101 70-71 -1.0688868319994853 1101 72-73 -1.4003590658390266 1101 74-75 -1.4320875892259508 1101 76 -2.019430501445207 1102 1 -0.27757377635589364 1102 2 -0.21635085863431414 1102 3 -0.21582236041403036 1102 4 -0.2665533509257543 1102 5 -0.0883526205078553 1102 6 -0.010633912055475037 1102 7 -0.09148606186911934 1102 8 -0.27867670597344585 1102 9 -0.10681046665205685 1102 10-11 -0.162395996502525 1102 12-13 -0.05697987536290583 1102 14-15 0.0621171282119235 1102 16-17 -0.025438312285437803 1102 18-19 -0.06778216617652788 1102 20-21 -0.005697058074566996 1102 22-23 -0.07296882783970204 1102 24-25 -0.023284897937102755 1102 26-27 -0.1612789210795711 1102 28-29 -0.06007650944066967 1102 30-31 0.013354698847777513 1102 32-33 -0.23173953352858945 1102 34-35 0.03244542695475161 1102 36-37 0.011440111671852549 1102 38-39 -0.18484963893143913 1102 40-41 0.026066642460691014 1102 42-43 0.028519267536758264 1102 44-45 0.028631130960114604 1102 46-47 0.06817757715070627 1102 48-49 -0.12805938107111814 1102 50-51 -0.1177706102635554 1102 52-53 0.04915990639268131 1102 54-55 0.1114247744602821 1102 56-57 -0.05048143231196178 1102 58-59 -0.037106375445276285 1102 60-61 -0.05117345181103161 1102 62-63 -0.015980824622992884 1102 64-65 -0.39195531734345934 1102 66-67 -0.16480708199049587 1102 68-69 -0.16639734528870065 1102 70-71 -0.3112523365537001 1102 72-73 -0.5343833849488178 1102 74-75 -0.5014778167128284 1102 76 -0.09031487350066669 1103 1 -0.12505439651093297 1103 2 -0.1828490049100111 1103 3 -0.010766748685007599 1103 4 -0.07606632699585703 1103 5 0.006768714173638557 1103 6 -0.07583445132073052 1103 7 -0.01739607198034321 1103 8 -0.13852672282548895 1103 9 0.05999944234693544 1103 10-11 0.008508957996973265 1103 12-13 0.07207304003986081 1103 14-15 0.025611398518627482 1103 16-17 0.04544136415540834 1103 18-19 0.23978018636813658 1103 20-21 0.1669183783932482 1103 22-23 0.13288725574641091 1103 24-25 -0.03528776278374579 1103 26-27 0.1676897341277055 1103 28-29 -0.046257837381347144 1103 30-31 -0.08895824351824899 1103 32-33 0.05166542097089888 1103 34-35 0.034800499418523145 1103 36-37 0.02967823098429534 1103 38-39 0.06953610424348255 1103 40-41 0.21209630204950258 1103 42-43 0.13378552975448343 1103 44-45 0.1108732947619373 1103 46-47 0.10972206653392647 1103 48-49 0.06263408033770901 1103 50-51 0.1060160429214747 1103 52-53 0.08882539341660589 1103 54-55 0.06835517553880521 1103 56-57 -0.14040391293211485 1103 58-59 0.10093710281559254 1103 60-61 0.07530952762947862 1103 62-63 0.06540188518489032 1103 64-65 0.13438863277450963 1103 66-67 -0.39732730443741815 1103 68-69 -0.16370944505277407 1103 70-71 -0.2352370011981222 1103 72-73 0.20072362549944955 1103 74-75 0.033658684803860695 1103 76 0.2727791608774979 1104 1 -0.025765369708778962 1104 2 -0.03717622307819113 1104 3 -0.013017311325668857 1104 4 -0.06388430421743863 1104 5 0.057943439673195485 1104 6 -0.05085661595279589 1104 7 -0.006595076276873613 1104 8 -0.2615319059394494 1104 9 0.12055151218257265 1104 10-11 -0.05047453787699396 1104 12-13 -0.005434973327119508 1104 14-15 0.0555720136724176 1104 16-17 0.030369414349777912 1104 18-19 0.09411166696555284 1104 20-21 0.06034059758441401 1104 22-23 -0.03115659612564059 1104 24-25 0.013325242740357623 1104 26-27 0.1764362389357217 1104 28-29 0.07779590332655317 1104 30-31 0.013992210004232675 1104 32-33 -0.0910790719705119 1104 34-35 -0.003505952194380768 1104 36-37 -0.03666648019350305 1104 38-39 -0.19207901544552897 1104 40-41 -0.23059959919854123 1104 42-43 -0.3502303605817403 1104 44-45 -0.21575239575160055 1104 46-47 -0.1468804363645262 1104 48-49 -0.015466410694138233 1104 50-51 -0.5681212413996022 1104 52-53 -0.1749465154697063 1104 54-55 -0.2353897947946777 1104 56-57 -0.4250659875416787 1104 58-59 -0.07391626961842235 1104 60-61 -0.09357644386339103 1104 62-63 -0.08934560850671147 1104 64-65 -0.27098970044515625 1104 66-67 -0.5693461614243844 1104 68-69 -0.4048180176504701 1104 70-71 -0.4986508660279725 1104 72-73 -0.10914219322796015 1104 74-75 -0.46819973434637774 1104 76 -0.2673899723148949 1105 1 0.02133819199227105 1105 2 0.03052481332199619 1105 3 -0.03685190498892155 1105 4 0.04567302846615462 1105 5 0.041540296535103494 1105 6 0.04149699578437094 1105 7 0.05953961223744386 1105 8 -0.22914331243283215 1105 9 -0.10959720384725813 1105 10-11 0.010691347066043022 1105 12-13 -0.04910562257832396 1105 14-15 -0.0030572578887984037 1105 16-17 -0.03564344710675016 1105 18-19 0.010590516918128401 1105 20-21 0.009251550707091383 1105 22-23 0.14814672714246058 1105 24-25 0.08641277548138504 1105 26-27 -0.04756456164208345 1105 28-29 0.23822908553632516 1105 30-31 -0.07289223703146064 1105 32-33 0.2072231809598648 1105 34-35 -0.038827097020643464 1105 36-37 -0.09184978640580965 1105 38-39 0.021588240051919172 1105 40-41 -0.039352873300714464 1105 42-43 -0.19346069203835015 1105 44-45 -0.23596698541197014 1105 46-47 -0.28544131073472556 1105 48-49 -0.20287123330524537 1105 50-51 -0.4831686234231469 1105 52-53 -0.023067333103305998 1105 54-55 -0.06966583750376998 1105 56-57 0.10392015027954216 1105 58-59 0.059023156228050766 1105 60-61 0.09150061175066071 1105 62-63 -0.02795347331348097 1105 64-65 -0.27474893537123535 1105 66-67 -0.23274670268460085 1105 68-69 9.531041156733977E-4 1105 70-71 0.054309096379675736 1105 72-73 0.22381985764353374 1105 74-75 0.3818920710435947 1105 76 0.5424292885429409 1106 1 -0.13952067901076504 1106 2 0.017123554601546687 1106 3 0.112303841971503 1106 4 0.005320446688713787 1106 5 -0.08442803412847866 1106 6 0.005372244158451167 1106 7 -2.714921628239608E-4 1106 8 0.22064354540528797 1106 9 0.11530060170756684 1106 10-11 0.11414626334100575 1106 12-13 0.11076412874123776 1106 14-15 -0.1350740991832282 1106 16-17 0.05802968822757748 1106 18-19 -0.1376680197099347 1106 20-21 0.009873032986099872 1106 22-23 -0.07035243692011761 1106 24-25 0.015730107834350804 1106 26-27 0.0687839236042791 1106 28-29 0.10517935820072921 1106 30-31 0.09838740373427868 1106 32-33 0.09554018944178466 1106 34-35 -0.007787996156693566 1106 36-37 0.04283680270686574 1106 38-39 0.07489128706573922 1106 40-41 0.05841241647633666 1106 42-43 -0.08130502385736804 1106 44-45 0.06033563269155451 1106 46-47 -0.1331562692004553 1106 48-49 0.1701450565330873 1106 50-51 0.1311841159131788 1106 52-53 0.06942411057377029 1106 54-55 0.231201611705373 1106 56-57 0.03771915765854317 1106 58-59 0.2502733860014317 1106 60-61 -0.045094190556703495 1106 62-63 0.05768853538726404 1106 64-65 0.10029811346326767 1106 66-67 0.14073351054509686 1106 68-69 0.16117736293240625 1106 70-71 -0.07704687291384715 1106 72-73 0.11977847217571735 1106 74-75 0.18295209222650755 1106 76 0.2324759434802246 1107 1 -0.016170267583966336 1107 2 -0.14157333511536763 1107 3 -0.19647326303289248 1107 4 0.03339579730715059 1107 5 -0.05278180942217148 1107 6 -0.10796419428278625 1107 7 0.091148059666601 1107 8 0.033120903993498985 1107 9 0.025200720736705762 1107 10-11 -0.12767695251574196 1107 12-13 -0.09445762771479593 1107 14-15 -0.08312823156433069 1107 16-17 -0.03377800208966164 1107 18-19 -0.04028407311778892 1107 20-21 -0.015524162066427039 1107 22-23 0.007275260642373382 1107 24-25 0.01359534615138358 1107 26-27 0.05215865837631384 1107 28-29 0.1482886062470996 1107 30-31 0.0028665218836323447 1107 32-33 0.025494130341229493 1107 34-35 -0.04962932514287388 1107 36-37 0.11179607404154268 1107 38-39 0.003823129947278403 1107 40-41 0.014319884260842741 1107 42-43 0.01283149738674183 1107 44-45 0.12036771880654129 1107 46-47 -0.2187408057630975 1107 48-49 -0.0935691700362895 1107 50-51 0.099913091745087 1107 52-53 -0.10161558305558316 1107 54-55 0.10572937654519876 1107 56-57 -0.10106622763360207 1107 58-59 0.003714880593371106 1107 60-61 -0.41950551385018997 1107 62-63 0.02637869737039722 1107 64-65 0.19484864909438215 1107 66-67 0.3016816034913248 1107 68-69 0.3153977220898838 1107 70-71 0.3742676227648829 1107 72-73 0.36804478693015596 1107 74-75 0.6294019188579867 1107 76 0.283115078190157 1108 1 -0.09966212976954836 1108 2 -0.0180969735286709 1108 3 -0.09531424097976782 1108 4 -0.1705956069061969 1108 5 -0.06912789552094978 1108 6 -0.2292387662548876 1108 7 -0.018201619752829856 1108 8 -0.29749982453464696 1108 9 -0.10688904182229919 1108 10-11 -0.09677263348523724 1108 12-13 -0.32467674981724315 1108 14-15 -0.13894800187364353 1108 16-17 -0.13244459829905963 1108 18-19 -0.16290614033402306 1108 20-21 -0.0239614198695719 1108 22-23 0.002598659500968381 1108 24-25 -0.1849620412931401 1108 26-27 -0.09005052925139267 1108 28-29 -0.010988854472969933 1108 30-31 -0.2909931272391759 1108 32-33 -0.3314776162944355 1108 34-35 -0.1376392596176217 1108 36-37 -0.03355726915579993 1108 38-39 -0.3923404718282413 1108 40-41 -0.14434107536713725 1108 42-43 -0.04150867030155325 1108 44-45 0.09877256626656106 1108 46-47 0.04494551848124928 1108 48-49 -0.015766732214828494 1108 50-51 -0.0717648480844062 1108 52-53 -0.023478473212705353 1108 54-55 -0.09823560619276606 1108 56-57 -0.0072083399424798245 1108 58-59 -0.0014725357386282667 1108 60-61 0.12542860860454397 1108 62-63 -0.18331974552398833 1108 64-65 0.08036439629370307 1108 66-67 0.060101246977545486 1108 68-69 0.11077220246753683 1108 70-71 0.18706266257323279 1108 72-73 -0.10246003939268178 1108 74-75 -0.02819337459653326 1108 76 -0.2024940248888285 1109 1 0.015993475106093058 1109 2 -0.20005286727911198 1109 3 -0.24618086537207517 1109 4 0.07140749321358442 1109 5 0.07002520812168456 1109 6 -0.24246711825939116 1109 7 -0.20417357776029377 1109 8 -0.025358628170245368 1109 9 -0.17947764183639237 1109 10-11 -0.05896350222334945 1109 12-13 -0.002352364556905684 1109 14-15 -0.27318671109649983 1109 16-17 -0.06594174477971393 1109 18-19 -0.04174606142188253 1109 20-21 0.06488519465871434 1109 22-23 -0.2090990083634665 1109 24-25 0.04429710053734226 1109 26-27 -0.14813373928451057 1109 28-29 -0.10463537036108761 1109 30-31 -0.0029814313327420905 1109 32-33 -0.12655265328449872 1109 34-35 -0.22068195672183322 1109 36-37 -0.22884720081225396 1109 38-39 0.06376465041510926 1109 40-41 0.05379356847137018 1109 42-43 0.05084319329317566 1109 44-45 -0.13840421101802036 1109 46-47 -0.10470571804380313 1109 48-49 -0.10672706477312488 1109 50-51 -0.2962857386642668 1109 52-53 -0.07968575849417903 1109 54-55 -0.09163904450743132 1109 56-57 -0.02942880073300813 1109 58-59 7.909039851838884E-4 1109 60-61 0.11996817036034457 1109 62-63 -0.2280072675418836 1109 64-65 0.11151531576104645 1109 66-67 -0.2275581625905474 1109 68-69 0.06685971039397742 1109 70-71 0.43274715492862725 1109 72-73 0.22038396821670858 1109 74-75 0.4349574744135438 1109 76 0.5989045518743694 1110 1 -0.009546565471538315 1110 2 0.10740796166399491 1110 3 0.14883729226691855 1110 4 0.08929234138844322 1110 5 0.014981135354737773 1110 6 -0.0743597750714926 1110 7 -0.03325307923507381 1110 8 0.03742588413091852 1110 9 0.10824847617973177 1110 10-11 -0.18402104403706687 1110 12-13 -0.0663431237678509 1110 14-15 -0.08399348994838363 1110 16-17 -0.036326891319532706 1110 18-19 0.09286768382558819 1110 20-21 0.0912691818375464 1110 22-23 -0.29854029866405085 1110 24-25 -0.02112154812513012 1110 26-27 0.027731092863888307 1110 28-29 -0.010492700761922436 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-0.1070643342787676 2115 56-57 0.2496183680872477 2115 58-59 0.2334221327557131 2115 60-61 0.14676545298807042 2115 62-63 0.12160053439809104 2115 64-65 0.0771629678253376 2115 66-67 0.2817302780719828 2115 68-69 0.37603084181534996 2115 70-71 -0.12220434557913507 2115 72-73 0.010960709471035557 2115 74-75 -0.05100918875135818 2115 76 0.026295384244711784 2116 1 0.08262522861922861 2116 2 0.005280116922683931 2116 3 -0.18778861609215625 2116 4 0.10143280524989251 2116 5 0.03169929383347636 2116 6 0.11088890862373546 2116 7 0.03051264027234879 2116 8 0.21122162844143588 2116 9 0.035953972024124425 2116 10-11 0.049284730298253976 2116 12-13 0.0607575245938321 2116 14-15 0.2947150083763148 2116 16-17 -0.21103208256447203 2116 18-19 0.08897751368503748 2116 20-21 0.019070408938191008 2116 22-23 0.10806080659681072 2116 24-25 -0.11071730113848588 2116 26-27 0.11380219507876177 2116 28-29 0.10719582454159848 2116 30-31 0.18512627574590113 2116 32-33 -0.0543871649367631 2116 34-35 0.06558408150807793 2116 36-37 0.037527068193590196 2116 38-39 0.23929052979016774 2116 40-41 0.12589359465623318 2116 42-43 0.09901461687701385 2116 44-45 0.04628200701463214 2116 46-47 0.28881352783208314 2116 48-49 -0.00869965794760219 2116 50-51 0.15484457468128454 2116 52-53 0.15199356419064003 2116 54-55 -0.02636453983779319 2116 56-57 0.21419518460346154 2116 58-59 0.2729520281887261 2116 60-61 0.2655472922945279 2116 62-63 0.29284461340426304 2116 64-65 -0.0035278892664507566 2116 66-67 -0.11367145577375481 2116 68-69 -0.18804995182125595 2116 70-71 0.321561853366255 2116 72-73 0.2544766626393269 2116 74-75 0.2817149152793874 2116 76 0.3247402854870032 2117 1 0.042931568639247075 2117 2 0.1540551919846891 2117 3 0.09635915815613316 2117 4 0.03389875538260867 2117 5 -0.03458154686100556 2117 6 0.09765188673608804 2117 7 0.07461864088154613 2117 8 -0.274527468638766 2117 9 -0.08125589699810831 2117 10-11 0.037553299986257116 2117 12-13 -0.10715945029846807 2117 14-15 -0.1116902829513009 2117 16-17 0.1910783564938754 2117 18-19 0.2021971298897256 2117 20-21 0.05919921099206249 2117 22-23 0.22769876839134184 2117 24-25 0.1785869066853607 2117 26-27 0.23181272551694576 2117 28-29 -0.18979299230711177 2117 30-31 0.1040506045392533 2117 32-33 -0.03062405628178766 2117 34-35 -0.035716993292851384 2117 36-37 0.06405768043848781 2117 38-39 0.15485121035237626 2117 40-41 -0.42148902576933267 2117 42-43 -0.035844614662089214 2117 44-45 0.16298275981041144 2117 46-47 0.10486156751704812 2117 48-49 0.04744237242530858 2117 50-51 0.2684253588072991 2117 52-53 -0.006846347040372791 2117 54-55 0.12415980636124146 2117 56-57 -0.09382448381827402 2117 58-59 -0.09111829077041023 2117 60-61 -0.058402927452220865 2117 62-63 0.03064105394209804 2117 64-65 0.22939931146459003 2117 66-67 0.237601334792366 2117 68-69 0.022667597458578825 2117 70-71 0.0321836713898449 2117 72-73 -0.5730025593266461 2117 74-75 -0.15257428014291463 2117 76 -0.7614265479841222 2118 1 -0.23240612349593448 2118 2 -0.04276368772272576 2118 3 -0.20761551709012593 2118 4 -0.036693133105671905 2118 5 -0.1355355379248877 2118 6 -0.019518227294305746 2118 7 0.1345751678710414 2118 8 0.08073500828776758 2118 9 0.1377997726070106 2118 10-11 0.0389032059672445 2118 12-13 0.075444445021283 2118 14-15 0.14967394152124314 2118 16-17 0.0076334654351697395 2118 18-19 -0.21094741787020155 2118 20-21 0.011042501139911565 2118 22-23 -0.04130773530904008 2118 24-25 0.10126273948679199 2118 26-27 -0.07649631238392374 2118 28-29 0.04132988795235093 2118 30-31 0.09362225737621088 2118 32-33 0.2695596689411772 2118 34-35 -0.06172316922052801 2118 36-37 -0.02874512115551653 2118 38-39 0.2265730520899183 2118 40-41 0.1635205486436888 2118 42-43 0.07875938416483308 2118 44-45 0.13969364703935838 2118 46-47 0.2532045902713662 2118 48-49 0.15504669902492907 2118 50-51 0.3922242443801238 2118 52-53 0.1764217192732147 2118 54-55 0.2720413596588074 2118 56-57 0.010070835932616262 2118 58-59 0.06321520932446845 2118 60-61 0.043711206451554574 2118 62-63 0.05197646891958385 2118 64-65 -0.11699605141429004 2118 66-67 -0.05359020522178071 2118 68-69 -0.10582792585640988 2118 70-71 0.12379182775467257 2118 72-73 -0.08723048211030004 2118 74-75 0.16600353137052792 2118 76 -0.030165658167504716 2119 1 0.14260970988263466 2119 2 0.11833867733164993 2119 3 0.08908390205107253 2119 4 0.007133658780404062 2119 5 0.07370029798702404 2119 6 0.09419257378417001 2119 7 0.164676682446796 2119 8 0.011258143115767894 2119 9 0.13137594472940606 2119 10-11 0.06311922753683064 2119 12-13 0.004474718441116465 2119 14-15 -0.3447436615766719 2119 16-17 -0.051777894787761625 2119 18-19 0.029851127268500477 2119 20-21 -0.11323594917946878 2119 22-23 0.17522955003558138 2119 24-25 -0.016623549712129204 2119 26-27 0.21937524724910418 2119 28-29 0.15877736637371953 2119 30-31 0.062217881935133335 2119 32-33 0.18215489540373397 2119 34-35 0.05869193672739215 2119 36-37 0.06474419357585504 2119 38-39 0.266699357212687 2119 40-41 0.09914203137174127 2119 42-43 0.07074769460762553 2119 44-45 -0.07001266640725845 2119 46-47 0.17748485011972548 2119 48-49 0.16788530713523642 2119 50-51 0.2543607552463456 2119 52-53 0.15499689615467815 2119 54-55 0.05580717041826233 2119 56-57 -0.1033866310887177 2119 58-59 0.10437746978806928 2119 60-61 0.09005776744172778 2119 62-63 0.07246486079117886 2119 64-65 -0.22797446373355967 2119 66-67 0.337439959626316 2119 68-69 0.28358271055493134 2119 70-71 0.5685242888950413 2119 72-73 0.9420160300226001 2119 74-75 0.44979196124223364 2119 76 -0.36775138460291146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 2.0 21 0.0 22 8.0 23 6.0 24 25.0 25 40.0 26 80.0 27 143.0 28 295.0 29 460.0 30 759.0 31 1291.0 32 2150.0 33 3708.0 34 6456.0 35 10889.0 36 16751.0 37 85356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.9484503971344 17.718423921507554 12.803301666407101 33.52982401495094 2 19.2633546176608 19.433110107459896 36.05201681981 25.251518455069306 3 18.27285469553029 19.743809375486684 32.68961220993615 29.293723719046877 4 14.369257125058402 23.12412396822925 33.93007319732129 28.57654570939106 5 14.549914343560195 30.84410527955147 38.96433577324404 15.641644603644291 6 27.4061672636661 38.4869957950475 17.38047033172403 16.726366609562373 7 25.960130820744432 25.841769194829467 23.081295748325807 25.1168042361003 8 22.38436380626071 36.88911384519545 17.765145615947674 22.961376732596168 9 21.87431864195608 16.815916523905933 18.694907335306027 42.61485749883196 10-11 25.28227690390905 26.83304781186731 24.439729014172247 23.444946270051396 12-13 24.32448216788662 20.29824014950942 26.39697866375954 28.98029901884442 14-15 21.72013705030369 19.94860613611587 21.52974614546021 36.80151066812023 16-17 26.39464257903753 26.00179099828687 20.546643824949385 27.056922597726214 18-19 28.036131443700356 24.539401962311167 21.586201526242018 25.83826506774646 20-21 23.889970409593523 22.85664226755957 21.293412241083942 31.959975081762966 22-23 23.369802211493536 25.944946270051396 21.1193739292945 29.565877589160568 24-25 26.302756579971962 26.254866843170845 22.815371437470798 24.627005139386387 26-27 26.836162591496652 28.85843326584644 21.810855007008254 22.494549135648654 28-29 26.30197788506463 24.656984893318796 22.248481544930694 26.792555676685875 30-31 24.746534807662357 22.226288740071638 29.387167107927116 23.64000934433889 32-33 21.211649275813734 29.428437938015882 26.13806260707055 23.221850179099828 34-35 21.571795670456314 28.302055754555365 23.28687120386233 26.83927737112599 36-37 26.70417380470332 26.820588693349944 22.9465815293568 23.52865597258994 38-39 22.21149353683227 24.731739604422987 27.655349634013394 25.401417224731347 40-41 21.47913097648341 20.951565176763744 28.865830867466123 28.703472979286715 42-43 25.1576857187354 20.79115402585267 28.262342314281263 25.788817941130667 44-45 20.860068525151846 25.163915277994082 30.7751907802523 23.200825416601774 46-47 23.68634169132534 22.2243420028033 28.532938794580282 25.55637751129108 48-49 23.48855318486217 20.939495405700047 25.8016664071017 29.770285002336085 50-51 21.563230026475626 21.750895499143436 28.659087369568603 28.026787104812335 52-53 18.06883662980844 21.685095779473603 35.02219280485905 25.223874785858904 54-55 18.60341068369413 22.655349634013394 31.040725743653635 27.700513938638842 56-57 22.059258682448217 23.486606447593832 26.346363494782743 28.107771375175204 58-59 19.367699735243733 23.639230649431553 30.956237346207754 26.03683226911696 60-61 22.14530446970877 26.646161034106836 26.84005606603333 24.368478430151068 62-63 18.95499143435602 33.16461610341068 23.214452577480145 24.665939884753154 64-65 18.2498831957639 35.18065721850179 21.89767948917614 24.67178009655817 66-67 18.390048279084255 34.71344027410061 21.67030057623423 25.226210870580907 68-69 18.09725899392618 35.12770596480299 21.69366142345429 25.08137361781654 70-71 18.555910294346674 33.83312568135805 22.122722317396043 25.488241706899238 72-73 18.79380158853761 31.56128328920729 22.89168353838966 26.75323158386544 74-75 18.930851892228624 31.12988631054353 23.209001713128796 26.73026008409905 76 18.78757202927893 31.42656907023828 23.325027254321757 26.460831646161033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 3.5 16 6.5 17 6.0 18 5.0 19 10.5 20 26.0 21 35.0 22 36.5 23 62.5 24 102.5 25 118.0 26 184.0 27 303.5 28 445.5 29 534.0 30 611.0 31 811.5 32 1181.5 33 1428.0 34 1580.0 35 2009.0 36 2473.5 37 2661.0 38 3113.0 39 5869.5 40 9405.5 41 10121.0 42 9605.0 43 8745.0 44 7748.5 45 7353.0 46 7094.0 47 7005.5 48 6554.5 49 5907.0 50 5622.0 51 5155.0 52 4229.0 53 3574.0 54 3378.0 55 3244.0 56 2896.5 57 2489.0 58 2295.0 59 2243.5 60 2229.5 61 2285.5 62 2304.0 63 2348.5 64 2338.0 65 2301.0 66 2261.0 67 2203.0 68 2120.0 69 1907.0 70 1647.5 71 1518.0 72 1337.0 73 974.5 74 631.0 75 469.0 76 339.0 77 136.0 78 38.0 79 13.0 80 11.5 81 6.0 82 2.0 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 128420.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.034885531848616 #Duplication Level Percentage of deduplicated Percentage of total 1 58.62952350228666 24.058557857031616 2 21.092282293109665 17.310387790063853 3 7.989069586503976 9.834916679644914 4 3.597927775774712 5.905622177230961 5 2.3018388143537583 4.722784612988631 6 1.5370893978784372 3.7844572496495874 7 1.0437026775717781 2.997975393240928 8 0.8463479894491147 2.778383429372372 9 0.6015522705277341 2.221616570627628 >10 2.326508150369091 14.09593521258371 >50 0.01707877108753819 0.5030369101386077 >100 0.00948820615974344 0.9048434823236257 >500 0.0 0.0 >1k 0.005692923695846064 4.913564865285782 >5k 0.001897641231948688 5.967917769817785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 7664 5.967917769817785 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 2332 1.8159165239059336 No Hit CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG 2013 1.567512848465971 Illumina PCR Primer Index 9 (96% over 25bp) TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 1965 1.5301354929138764 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 485 0.3776670300576234 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT 235 0.18299330322379692 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 193 0.15028811711571408 Illumina PCR Primer Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT 140 0.10901728702694286 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012459118517364896 0.0 2 0.0 0.0 0.0 0.017909982868712038 0.0 3 0.0 0.0 0.0 0.01946737268338265 0.0 4 0.0 0.0 0.0 0.06307428749415979 0.0 5 0.0 0.0 0.0 0.06930384675284224 0.0 6 0.0 0.0 0.0 0.07008254166017754 0.0 7 0.0 0.0 0.0 0.07008254166017754 0.0 8 0.0 0.0 0.0 0.07008254166017754 0.0 9 0.0 0.0 0.0 0.07864818564086591 0.0 10 0.0 0.0 0.0 0.07942688054820121 0.0 11 0.0 0.0 0.0 0.08020557545553653 0.0 12 0.0 0.0 0.0 0.08020557545553653 0.0 13 0.0 0.0 0.0 0.08020557545553653 0.0 14 0.0 0.0 0.0 0.08020557545553653 0.0 15 0.0 0.0 0.0 0.08254166017754244 0.0 16 0.0 0.0 0.0 0.08409904999221306 0.0 17 0.0 0.0 0.0 0.08409904999221306 0.0 18 0.0 0.0 0.0 0.08409904999221306 0.0 19 0.0 0.0 0.0 0.08877121943622489 0.0 20 0.0 0.0 0.0 0.08877121943622489 0.0 21 0.0 0.0 0.0 0.0911073041582308 0.0 22 0.0 0.0 0.0 0.09889425323158386 0.0 23 0.0 0.0 0.0 0.1035664226755957 0.0 24 0.0 0.0 0.0 0.11135337174894876 0.0 25 0.0 0.0 0.0 0.11991901572963713 0.0 26 0.0 0.0 0.0 0.1300420495249961 0.0 27 0.0 0.0 0.0 0.21491979442454445 0.0 28 0.0 0.0 0.0 0.3099205731194518 0.0 29 0.0 0.0 0.0 0.40570004672169446 0.0 30 0.0 0.0 0.0 0.5123812490266314 0.0 31 0.0 0.0 0.0 0.6393085189222862 0.0 32 0.0 0.0 0.0 0.8004983647406946 0.0 33 0.0 0.0 0.0 1.0068525151845507 0.0 34 0.0 0.0 0.0 1.2809531225665785 0.0 35 0.0 0.0 0.0 1.6671857966048902 0.0 36 0.0 0.0 0.0 2.2099361470175984 0.0 37 0.0 0.0 0.0 2.8975237501946736 0.0 38 0.0 0.0 0.0 3.627939573275191 0.0 39 0.0 0.0 0.0 4.338888023672325 0.0 40 0.0 0.0 0.0 5.146394642579038 0.0 41 0.0 0.0 0.0 6.058246379068681 0.0 42 0.0 0.0 0.0 6.987229403519701 0.0 43 0.0 0.0 0.0 8.021336240460988 0.0 44 0.0 0.0 0.0 9.006385298240149 0.0 45 0.0 0.0 0.0 10.022582152312724 0.0 46 0.0 0.0 0.0 11.066033328142034 0.0 47 0.0 0.0 0.0 11.940507709079583 0.0 48 0.0 0.0 0.0 12.64289051549603 0.0 49 0.0 0.0 0.0 13.431708456626694 0.0 50 0.0 0.0 0.0 14.187821211649275 0.0 51 0.0 0.0 0.0 14.918237034729794 0.0 52 0.0 0.0 0.0 15.717178009655816 0.0 53 0.0 0.0 0.0 16.600218034574056 0.0 54 0.0 0.0 0.0 17.3555520946893 0.0 55 0.0 0.0 0.0 18.14670612054197 0.0 56 0.0 0.0 0.0 18.83740850334839 0.0 57 0.0 0.0 0.0 19.518766547266782 0.0 58 0.0 0.0 0.0 20.154960286559724 0.0 59 0.0 0.0 0.0 20.772465348076622 0.0 60 0.0 0.0 0.0 21.460052951253697 0.0 61 0.0 0.0 0.0 22.099361470175985 0.0 62 0.0 0.0 0.0 22.759694751596324 0.0 63 0.0 0.0 0.0 23.418470643201992 0.0 64 0.0 0.0 0.0 24.113066500545088 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTGAT 20 0.0069380524 52.5 37 GCTTATA 20 0.0069380524 52.5 3 GTCAATT 20 0.0069380524 52.5 12 AAGCTGG 20 0.0069380524 52.5 70 CTTTATA 35 2.0770318E-5 50.000004 2 CAGCAAA 30 5.8598054E-4 46.666664 20 TATACCG 30 5.8598054E-4 46.666664 6 TAAGCGC 30 5.8598054E-4 46.666664 47 TTCAGCA 35 0.0012516078 40.000004 18 GTAAGCG 35 0.0012516078 40.000004 46 CCCAGGG 50 1.7020512E-4 35.000004 2 TCTTTAT 50 1.7020512E-4 35.000004 1 TCTTCAG 40 0.0024114659 35.0 16 CCATCGT 40 0.0024114659 35.0 31 TCAGCAA 40 0.0024114659 35.0 19 TATGCCG 1725 0.0 34.492752 43 CGTATGC 1725 0.0 34.289852 41 GTATGCC 1725 0.0 34.289852 42 TCGTATG 1725 0.0 34.289852 40 CTCGTAT 1715 0.0 34.285713 39 >>END_MODULE