FastQCFastQC Report
Wed 25 May 2016
SRR1780227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564402
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA401.8090464E-533.0247713
AATGCGG353.2367464E-431.39363742
AAACTCG502.3724842E-630.7766727
CGAAACT554.9841856E-627.9787925
TCGGAGT407.06596E-427.474331
TATCGGT508.2912506E-526.41981324
TTCTAGC757.080416E-826.3683192
TCTAGCA801.3236422E-724.718113
ATTGGCG551.5793012E-424.02867737
ATCGGTT551.6003528E-423.98182125
TCTAGCC551.6061352E-423.9690723
TATCGGC602.8402192E-422.03411515
GTATCGG602.8556163E-422.0165114
ATTGTTC500.002598127821.9716518
CTTACAT500.002598127821.9716513
AACCGTA654.9292814E-420.3067110
GACGAAA1152.0082553E-719.13799723
TAGATCT1504.5656634E-1019.08944939
GGAAAGA708.031848E-418.88638516
TTTCAAC708.176907E-418.83618242
Produced by FastQC (version 0.11.5)