Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780227_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 564402 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 56 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT | 18462 | 3.271072746021453 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC | 5983 | 1.060060028135974 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG | 4478 | 0.7934061183340952 | No Hit |
CACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTG | 4046 | 0.7168649296069114 | RNA PCR Primer, Index 36 (95% over 24bp) |
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC | 1041 | 0.18444300339119987 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCTTCTG | 500 | 0.0 | 37.399696 | 44 |
TTAATAC | 30 | 1.2989623E-4 | 36.67287 | 2 |
CATCGTT | 25 | 0.002352849 | 35.196598 | 32 |
GTCTTCT | 555 | 0.0 | 33.69342 | 43 |
CGTCTTC | 560 | 0.0 | 33.39259 | 42 |
CCGTCTT | 560 | 0.0 | 33.39259 | 41 |
CTTTATA | 135 | 0.0 | 32.598106 | 2 |
ATGCCGT | 2875 | 0.0 | 32.368435 | 44 |
TTATCGG | 35 | 3.217529E-4 | 31.425535 | 25 |
TATCGGA | 35 | 3.217529E-4 | 31.425535 | 26 |
TCTTTAT | 145 | 0.0 | 30.422749 | 1 |
GCCGTCT | 625 | 0.0 | 30.271757 | 40 |
CGCAATC | 30 | 0.005745165 | 29.3305 | 12 |
AATCGAT | 30 | 0.005745165 | 29.3305 | 22 |
TATGCCG | 3285 | 0.0 | 28.328539 | 43 |
ATCGTAT | 40 | 7.031427E-4 | 27.497343 | 39 |
ATCGGAA | 40 | 7.031427E-4 | 27.497343 | 27 |
CTATAAC | 65 | 5.913025E-7 | 27.081503 | 4 |
TATCTAG | 45 | 0.0013966357 | 24.446413 | 5 |
TTCGGTA | 45 | 0.0013980784 | 24.442081 | 26 |