FastQCFastQC Report
Wed 25 May 2016
SRR1780227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564402
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT184623.271072746021453No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC59831.060060028135974No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG44780.7934061183340952No Hit
CACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTG40460.7168649296069114RNA PCR Primer, Index 36 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC10410.18444300339119987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG5000.037.39969644
TTAATAC301.2989623E-436.672872
CATCGTT250.00235284935.19659832
GTCTTCT5550.033.6934243
CGTCTTC5600.033.3925942
CCGTCTT5600.033.3925941
CTTTATA1350.032.5981062
ATGCCGT28750.032.36843544
TTATCGG353.217529E-431.42553525
TATCGGA353.217529E-431.42553526
TCTTTAT1450.030.4227491
GCCGTCT6250.030.27175740
CGCAATC300.00574516529.330512
AATCGAT300.00574516529.330522
TATGCCG32850.028.32853943
ATCGTAT407.031427E-427.49734339
ATCGGAA407.031427E-427.49734327
CTATAAC655.913025E-727.0815034
TATCTAG450.001396635724.4464135
TTCGGTA450.001398078424.44208126