FastQCFastQC Report
Wed 25 May 2016
SRR1780226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138018
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTC252.3836464E-455.99999628
TTCTGAT252.3836464E-455.99999619
GACAGTG200.00693914652.525
GGAGCAA200.00693914652.526
CAAAAGA200.00693914652.51
GAACTAA200.00693914652.519
TGACATT200.00693914652.522
GAGCAAA200.00693914652.528
TTACCGG200.00693914652.542
ACGAATT200.00693914652.524
CATTACA352.0778481E-550.00000461
CGGTTGA305.8613386E-446.66666456
GACGCTT305.8613386E-446.66666414
TTTTCGG305.8613386E-446.66666426
TACATAT404.5737175E-543.755
TTCCGAC503.4585955E-642.019
ACGCTTC503.4585955E-642.015
CTGAAGT350.001251933340.00000438
TTGGTAG350.001251933340.00000467
TAAGACC350.001251933340.00000460
Produced by FastQC (version 0.11.5)