##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780226_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 138018 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.547566259473403 34.0 32.0 34.0 21.0 34.0 2 30.58785086003275 34.0 32.0 34.0 12.0 34.0 3 30.42959613963396 34.0 32.0 34.0 11.0 34.0 4 30.25879233143503 34.0 32.0 34.0 11.0 34.0 5 30.328696257009955 34.0 32.0 34.0 11.0 34.0 6 33.28613659087945 38.0 34.0 38.0 11.0 38.0 7 33.73790375168456 38.0 34.0 38.0 21.0 38.0 8 33.624135982263184 38.0 34.0 38.0 21.0 38.0 9 33.871726876204555 38.0 34.0 38.0 21.0 38.0 10-11 33.65921836282224 38.0 34.0 38.0 21.0 38.0 12-13 33.72844121781217 38.0 34.0 38.0 21.0 38.0 14-15 33.64823791099711 38.0 34.0 38.0 21.0 38.0 16-17 33.71180570650205 38.0 34.0 38.0 21.0 38.0 18-19 33.72458664811836 38.0 34.0 38.0 21.0 38.0 20-21 33.72079728731035 38.0 34.0 38.0 21.0 38.0 22-23 33.72092045965019 38.0 34.0 38.0 21.0 38.0 24-25 33.73626990682375 38.0 34.0 38.0 21.0 38.0 26-27 33.71118622208697 38.0 34.0 38.0 21.0 38.0 28-29 33.7923169441667 38.0 35.0 38.0 21.0 38.0 30-31 33.78562216522482 38.0 34.5 38.0 21.0 38.0 32-33 33.849352982944254 38.0 35.0 38.0 21.0 38.0 34-35 33.81184338274718 38.0 34.5 38.0 21.0 38.0 36-37 33.74882986277152 38.0 34.0 38.0 21.0 38.0 38-39 33.744214522743405 38.0 34.5 38.0 21.0 38.0 40-41 33.79898274138156 38.0 34.5 38.0 21.0 38.0 42-43 33.87529162862815 38.0 35.0 38.0 21.0 38.0 44-45 33.95204248721181 38.0 35.0 38.0 21.0 38.0 46-47 33.874324363488824 38.0 35.0 38.0 21.0 38.0 48-49 33.83545986755351 38.0 35.0 38.0 21.0 38.0 50-51 33.7877160950021 38.0 35.0 38.0 21.0 38.0 52-53 33.858532944978194 38.0 35.0 38.0 21.0 38.0 54-55 33.81559289368053 38.0 35.0 38.0 21.0 38.0 56-57 33.77121824689533 38.0 35.0 38.0 21.0 38.0 58-59 33.83536567694069 38.0 35.0 38.0 21.0 38.0 60-61 33.861221000159404 38.0 35.0 38.0 20.5 38.0 62-63 33.72979611355041 38.0 35.0 38.0 20.0 38.0 64-65 33.69524627222536 38.0 34.5 38.0 19.0 38.0 66-67 33.632493587792894 38.0 34.0 38.0 19.0 38.0 68-69 33.58347099653668 38.0 34.0 38.0 19.0 38.0 70-71 33.572943384196265 38.0 34.0 38.0 19.0 38.0 72-73 33.634830239533976 38.0 34.0 38.0 19.0 38.0 74-75 33.63481574867046 38.0 34.0 38.0 19.0 38.0 76 32.05629700473851 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09157633093570894 1101 2 -0.2379092767858637 1101 3 -0.22259553387961617 1101 4 0.10027707431574129 1101 5 0.35635249368746 1101 6 0.06761528275116291 1101 7 -0.1881707436622193 1101 8 -0.19615046679597725 1101 9 -0.1856828269441806 1101 10-11 -0.07781369894339463 1101 12-13 -0.2096190145315333 1101 14-15 0.0027392605974014828 1101 16-17 -0.05993368714542413 1101 18-19 0.08896372630400151 1101 20-21 0.19170708489069455 1101 22-23 0.25257931281577584 1101 24-25 0.32190943531868754 1101 26-27 -0.07091837194916195 1101 28-29 0.09055149518692218 1101 30-31 -0.2923048588504713 1101 32-33 0.1574117922193139 1101 34-35 0.19363315016872207 1101 36-37 -0.005313004970474822 1101 38-39 -0.015707832231932173 1101 40-41 0.16791140498847312 1101 42-43 -0.3176723827407528 1101 44-45 0.22757187938747592 1101 46-47 0.027343125756395636 1101 48-49 0.10728247549712933 1101 50-51 0.011704781742430725 1101 52-53 0.06695911690979273 1101 54-55 0.22040954512244326 1101 56-57 0.27943473335392355 1101 58-59 0.06867788202949754 1101 60-61 0.19144187015003666 1101 62-63 0.16857922586660123 1101 64-65 0.34171800388407547 1101 66-67 -0.12018248149097133 1101 68-69 0.08683706656212564 1101 70-71 0.11182583141949465 1101 72-73 0.21093191763365127 1101 74-75 -0.2241826653029051 1101 76 -1.058911596649473 1102 1 0.6656796057397543 1102 2 0.42699837492917325 1102 3 0.7431036745636561 1102 4 0.6701979186429199 1102 5 0.11096990793548045 1102 6 0.663921351352748 1102 7 0.17330682889715376 1102 8 0.7378864725180065 1102 9 0.8011245609186162 1102 10-11 0.5290464593679545 1102 12-13 0.8800907480014573 1102 14-15 0.8932405798586203 1102 16-17 0.5983776585010148 1102 18-19 0.24067876588183879 1102 20-21 0.42653558093290656 1102 22-23 0.39901730753872755 1102 24-25 0.36148727173029727 1102 26-27 0.12697081010888667 1102 28-29 0.3003140281684509 1102 30-31 0.5243178324424065 1102 32-33 0.43181812467314273 1102 34-35 0.23057246415289256 1102 36-37 0.3904653591456224 1102 38-39 0.4143713234408892 1102 40-41 0.30247604878793766 1102 42-43 0.31161521620383326 1102 44-45 0.39247953110251643 1102 46-47 -0.03466215128845107 1102 48-49 -0.011451033737699845 1102 50-51 -0.018638226173131045 1102 52-53 0.3189380087303775 1102 54-55 0.013285534568360902 1102 56-57 0.44566164628268723 1102 58-59 0.2388625785994165 1102 60-61 0.025214957221280088 1102 62-63 -0.008201776771926461 1102 64-65 0.14514808303975002 1102 66-67 0.18720538130586561 1102 68-69 0.1250956417600122 1102 70-71 0.20417411637991734 1102 72-73 0.4958923397972441 1102 74-75 0.29296773047546054 1102 76 0.5189248149600232 1103 1 -0.04400111259286277 1103 2 0.12516368142730272 1103 3 -0.0013891768813962813 1103 4 0.39496662181255715 1103 5 0.3191881894187816 1103 6 -0.16044305109350887 1103 7 0.06304795371221417 1103 8 0.35731881521230235 1103 9 0.573727562152655 1103 10-11 0.007723516495026672 1103 12-13 0.5839183099930949 1103 14-15 0.3145382965481289 1103 16-17 0.23204915904985768 1103 18-19 0.19833286718200327 1103 20-21 -0.11644438129599877 1103 22-23 0.14608050253713145 1103 24-25 0.08429242332601916 1103 26-27 -0.2016172735875017 1103 28-29 0.10014767171566774 1103 30-31 0.3086510089643255 1103 32-33 0.23372099704910454 1103 34-35 0.02956750754874804 1103 36-37 0.11982250054178678 1103 38-39 -0.32441836518619027 1103 40-41 0.0790811499313051 1103 42-43 -0.10584062093047208 1103 44-45 0.06994232248197818 1103 46-47 0.017041297880787454 1103 48-49 0.15751326098593665 1103 50-51 0.008452649448145166 1103 52-53 0.3328976332011422 1103 54-55 -0.13401492291188788 1103 56-57 0.22843693553156186 1103 58-59 -0.20109569592963794 1103 60-61 0.06696429077278765 1103 62-63 0.166128195342921 1103 64-65 0.04521489833636139 1103 66-67 1.2595973543483296E-4 1103 68-69 0.26545208091377503 1103 70-71 -0.036231411563193205 1103 72-73 -0.04049131636305958 1103 74-75 0.10786549625375841 1103 76 0.5659205128730704 1104 1 0.16257755910208616 1104 2 -0.2681738421427795 1104 3 -0.2048461221988056 1104 4 -0.4150620114718926 1104 5 -0.2489031577423333 1104 6 -0.31470661698498503 1104 7 0.24764184993401983 1104 8 -0.030857733429364487 1104 9 -0.15123228141184342 1104 10-11 -0.007518469677187056 1104 12-13 -0.005696886598848039 1104 14-15 0.35122032741620757 1104 16-17 -0.20958782677318055 1104 18-19 -0.3287500296683348 1104 20-21 -0.003971443241667316 1104 22-23 -0.32396279657594107 1104 24-25 -0.02281351982642832 1104 26-27 -0.06374800897404498 1104 28-29 -0.24827400577578373 1104 30-31 -0.09538907763389659 1104 32-33 -0.18322501873412023 1104 34-35 0.008309039778644944 1104 36-37 -0.1383694123012873 1104 38-39 -0.29248526929960406 1104 40-41 -0.2542521834020306 1104 42-43 0.009418824561528538 1104 44-45 0.5346744965164802 1104 46-47 0.19318139146273694 1104 48-49 -0.26584381702230786 1104 50-51 -0.1098063071829074 1104 52-53 -0.14664353587490808 1104 54-55 -0.20088760955201934 1104 56-57 0.09890275018341299 1104 58-59 -0.03871354633818669 1104 60-61 -0.08252589708949642 1104 62-63 0.001699813472676226 1104 64-65 -0.11175486212177788 1104 66-67 0.1443329574307981 1104 68-69 0.008419466904420858 1104 70-71 -0.06530816273296125 1104 72-73 0.2692789189885687 1104 74-75 0.11601201486539026 1104 76 -0.09346910930725016 1105 1 0.6507145510558736 1105 2 0.4507450767761405 1105 3 0.028198372677749717 1105 4 0.7741409319295265 1105 5 0.731875330669741 1105 6 0.9379651387402106 1105 7 1.1843174568386345 1105 8 0.4503815210923321 1105 9 0.6312654835286722 1105 10-11 0.67009998847562 1105 12-13 -0.5065702790752908 1105 14-15 0.8399934516855083 1105 16-17 0.3401817901720108 1105 18-19 0.4991297585168013 1105 20-21 0.3677361422063825 1105 22-23 0.6342553452299882 1105 24-25 0.2915630705043526 1105 26-27 0.5702627193114864 1105 28-29 0.5666940156807883 1105 30-31 0.5766451703545385 1105 32-33 0.4628962934177281 1105 34-35 0.6831663398267906 1105 36-37 0.6174554965770582 1105 38-39 0.6008336221108905 1105 40-41 0.6070709718142524 1105 42-43 0.6453379497936282 1105 44-45 0.5080944813868413 1105 46-47 0.7799464007468302 1105 48-49 0.6916178431111106 1105 50-51 0.4672963680113682 1105 52-53 0.5163404404678502 1105 54-55 0.3784019853864393 1105 56-57 0.21190735784451675 1105 58-59 0.3852703612335091 1105 60-61 0.11147382782626636 1105 62-63 0.42103479731147786 1105 64-65 0.3263064816647585 1105 66-67 0.6265831465512193 1105 68-69 0.5740304977826227 1105 70-71 0.37576535353139207 1105 72-73 0.6892362809306647 1105 74-75 0.6772285749369118 1105 76 0.47204908063842055 1106 1 0.2295083642527871 1106 2 0.3695932443154071 1106 3 0.8126860696789358 1106 4 0.44816142138504844 1106 5 0.0866706359466427 1106 6 1.0915761055281052 1106 7 0.6170206528206776 1106 8 0.44769044826018245 1106 9 1.060611499541885 1106 10-11 0.46973496176472196 1106 12-13 0.910406127409928 1106 14-15 0.8134390906820883 1106 16-17 0.9364642462422452 1106 18-19 0.9022744503090223 1106 20-21 0.8814113217222541 1106 22-23 0.6887229257956449 1106 24-25 0.5235215587075999 1106 26-27 0.7635903241234416 1106 28-29 0.5315335504715719 1106 30-31 0.6820766079469678 1106 32-33 0.5501130313578528 1106 34-35 0.7333974790032158 1106 36-37 0.5978552940804889 1106 38-39 0.7896217067864342 1106 40-41 0.7562190207864461 1106 42-43 0.6849246969260463 1106 44-45 0.36140436774896756 1106 46-47 0.9144634064620263 1106 48-49 0.7116077182826359 1106 50-51 0.5566080128963407 1106 52-53 0.546918088576767 1106 54-55 0.6330447521290381 1106 56-57 0.4995890385660431 1106 58-59 0.8119019906647864 1106 60-61 0.8305065070423865 1106 62-63 0.6347619331840093 1106 64-65 0.9754745138510614 1106 66-67 0.6539268445116022 1106 68-69 0.6946158727343317 1106 70-71 0.6066247649591219 1106 72-73 0.6968931824667166 1106 74-75 0.3527178306287979 1106 76 0.8545144763726711 1107 1 0.14671696166254122 1107 2 -0.13609702848701843 1107 3 0.05589356485667096 1107 4 0.022989197631947178 1107 5 -0.13226019899411057 1107 6 -0.43312766961656024 1107 7 6.005761538574461E-5 1107 8 -0.11549500227715725 1107 9 0.41000527193097724 1107 10-11 0.2929438347324833 1107 12-13 1.1178806382944728 1107 14-15 0.03799130892402758 1107 16-17 0.08842070807745728 1107 18-19 0.29745907417235173 1107 20-21 0.6065904344254776 1107 22-23 0.6500414708493807 1107 24-25 0.2982391117525083 1107 26-27 0.7328380507698995 1107 28-29 0.5448981137121294 1107 30-31 0.12893524669042478 1107 32-33 0.2666327337552161 1107 34-35 0.49379979369045657 1107 36-37 0.5796960215536231 1107 38-39 -0.002869337578410125 1107 40-41 0.7286425534400749 1107 42-43 0.0810048843054858 1107 44-45 0.6667157482945711 1107 46-47 0.35399220227355244 1107 48-49 0.7286440919392874 1107 50-51 0.3241197241115472 1107 52-53 0.44226115829294343 1107 54-55 0.25473116711939525 1107 56-57 0.5308373163285083 1107 58-59 0.5006178903616743 1107 60-61 0.3547073888279968 1107 62-63 0.5783000979676984 1107 64-65 0.7154855361712507 1107 66-67 0.37473836283620443 1107 68-69 0.4418120700338619 1107 70-71 0.4837672284476895 1107 72-73 0.3562277098847346 1107 74-75 0.3787787289478928 1107 76 0.6541838067667172 1108 1 0.07008991016282451 1108 2 -0.11914739634213944 1108 3 -0.004264895811129321 1108 4 0.5588409237213661 1108 5 -0.07184706939592189 1108 6 0.2906697271010401 1108 7 -0.09326195709881802 1108 8 0.24056045895657263 1108 9 -0.351086974814919 1108 10-11 0.17160823767532207 1108 12-13 -0.09039610194322023 1108 14-15 0.12970042041242635 1108 16-17 0.23005907819630522 1108 18-19 0.3830561313313723 1108 20-21 0.10141216617419957 1108 22-23 0.26131764515611167 1108 24-25 -0.002114595095250138 1108 26-27 0.013780325812348337 1108 28-29 -0.0010130351277126692 1108 30-31 0.23458040798074364 1108 32-33 -0.11820031661264352 1108 34-35 0.056688871222995374 1108 36-37 0.002418149454015861 1108 38-39 0.005393254385367641 1108 40-41 0.31564028971624936 1108 42-43 0.24151705182104877 1108 44-45 0.049822709245333385 1108 46-47 0.1445836001230063 1108 48-49 -0.0629376850839094 1108 50-51 0.4417484734041395 1108 52-53 0.3309283908905698 1108 54-55 -0.15807302076968455 1108 56-57 -0.13919663952887618 1108 58-59 0.5271176073961925 1108 60-61 0.42588735487213825 1108 62-63 -0.05146787034016853 1108 64-65 0.5427351117230401 1108 66-67 0.3153297606856711 1108 68-69 0.21120935356527326 1108 70-71 0.09068573438996452 1108 72-73 -5.412373156588046E-5 1108 74-75 0.15786758010805357 1108 76 0.36089008860033545 1109 1 0.20528168054636708 1109 2 0.44024638911209735 1109 3 0.5980126420772827 1109 4 0.247944301928122 1109 5 0.17434292298406007 1109 6 0.7196765851147546 1109 7 0.035071799568690665 1109 8 -0.37909494702090996 1109 9 0.49338004745245456 1109 10-11 0.3454821687565186 1109 12-13 -0.007782436715892516 1109 14-15 0.27647038061900986 1109 16-17 0.45778111697371315 1109 18-19 0.3919990658839083 1109 20-21 0.40512269026191916 1109 22-23 0.17008912936574916 1109 24-25 0.19070354054102268 1109 26-27 0.659671867595435 1109 28-29 0.39843451376738415 1109 30-31 0.49153367188727515 1109 32-33 0.4513958225796344 1109 34-35 0.4065639876398919 1109 36-37 0.44509870148178976 1109 38-39 0.24087289201131057 1109 40-41 0.2759315436320904 1109 42-43 0.5694528743593565 1109 44-45 0.07730768088194395 1109 46-47 0.23798170358588067 1109 48-49 0.3296836001586527 1109 50-51 0.41310991612701997 1109 52-53 0.14367709972130882 1109 54-55 0.2416285286334201 1109 56-57 0.1365632453242256 1109 58-59 0.4263534892012899 1109 60-61 0.2572733509774707 1109 62-63 0.6879699418687863 1109 64-65 0.7093382782626989 1109 66-67 0.6518381003690124 1109 68-69 0.3382307742747841 1109 70-71 0.6529687756227105 1109 72-73 0.35246683131034473 1109 74-75 0.5008846828089304 1109 76 0.318916504653167 1110 1 -0.20590054467013275 1110 2 0.13791085217721033 1110 3 0.28240637093604093 1110 4 -0.2744636591088323 1110 5 -0.05978623333129107 1110 6 0.05534685895084834 1110 7 0.01333053326975886 1110 8 -0.45883865552793424 1110 9 0.14103538732641852 1110 10-11 0.16566407115241333 1110 12-13 0.17073034276955923 1110 14-15 -0.22982462127580305 1110 16-17 0.08345307509124922 1110 18-19 0.07655420504766397 1110 20-21 -0.34245966408282413 1110 22-23 -0.14762951141912595 1110 24-25 -3.5362677830619305E-4 1110 26-27 0.131923330814935 1110 28-29 -0.08928426737427486 1110 30-31 -0.04459416507428671 1110 32-33 0.07447118013855203 1110 34-35 -0.051865840984469 1110 36-37 0.21255557685899618 1110 38-39 -0.08627772485677099 1110 40-41 0.011790597346632126 1110 42-43 0.0058923033879523246 1110 44-45 0.4043811015171954 1110 46-47 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8 0.3691110214526887 2116 9 -0.3039740405119815 2116 10-11 0.16120516725454337 2116 12-13 0.11741668940490513 2116 14-15 0.2337777403204342 2116 16-17 0.21791568830258967 2116 18-19 0.353323083907668 2116 20-21 0.30113713354320737 2116 22-23 -0.12798560551204474 2116 24-25 0.31562578653195317 2116 26-27 0.5053661919320547 2116 28-29 -0.029409765813277033 2116 30-31 0.40465779004302505 2116 32-33 0.3420054140107638 2116 34-35 0.42892300634637337 2116 36-37 -0.17731645182636413 2116 38-39 0.08546348870695653 2116 40-41 -0.09818281924017924 2116 42-43 0.24038273645030017 2116 44-45 -0.13619724227433494 2116 46-47 0.10196908088627055 2116 48-49 0.45157533623499546 2116 50-51 0.5718684311392295 2116 52-53 -0.029247816456070552 2116 54-55 0.2582906438458892 2116 56-57 0.006057882194042463 2116 58-59 0.44432035461478847 2116 60-61 -0.03066681257145376 2116 62-63 -0.045003826583567275 2116 64-65 -0.3549434264086031 2116 66-67 -0.11818071230830185 2116 68-69 0.0828297153031059 2116 70-71 0.2111173957735133 2116 72-73 0.11263933142412696 2116 74-75 0.05577142745717367 2116 76 0.02138794427812485 2117 1 -0.37692039582613646 2117 2 -0.4335279202565019 2117 3 0.06913735682918443 2117 4 -0.11481339490280007 2117 5 -0.08204936032473853 2117 6 -0.5233119582312753 2117 7 0.0962102996894103 2117 8 0.02562634709032352 2117 9 -0.22776608524534936 2117 10-11 0.1676158634690168 2117 12-13 0.18980395548953055 2117 14-15 -0.23649721208175123 2117 16-17 -0.08140593568327148 2117 18-19 0.32313063437850076 2117 20-21 -0.3143411601395769 2117 22-23 -0.15245345340487404 2117 24-25 -7.066949006144796E-4 2117 26-27 0.17996439779473405 2117 28-29 -0.13903910321743496 2117 30-31 -0.08308255746388227 2117 32-33 0.07679984292279585 2117 34-35 0.021052643916910085 2117 36-37 0.013250605342470578 2117 38-39 0.06915159011172989 2117 40-41 0.1463412884080526 2117 42-43 0.25980778919381464 2117 44-45 0.3188592747665382 2117 46-47 0.0545856740836399 2117 48-49 -0.41180179280223683 2117 50-51 0.03056982562646482 2117 52-53 7.259404263137981E-4 2117 54-55 -0.22253730078627854 2117 56-57 -0.44574397369179053 2117 58-59 0.297869178820207 2117 60-61 -0.24688951887151234 2117 62-63 0.03461744920930698 2117 64-65 -0.2794387180845632 2117 66-67 0.09662263583323494 2117 68-69 0.005528296802566501 2117 70-71 0.203265900944956 2117 72-73 -1.5440260774024779 2117 74-75 -0.40152347314487713 2117 76 0.030101345420476378 2118 1 -0.37762514360061417 2118 2 -0.37112992322385807 2118 3 -0.14673801409069753 2118 4 -0.8128660655259452 2118 5 -0.219595725309901 2118 6 -0.12095661698498361 2118 7 -0.3604662581740925 2118 8 -0.07519894964558205 2118 9 -0.18045525438481747 2118 10-11 -1.2290134021096222 2118 12-13 -0.6644384406529014 2118 14-15 -0.5905448752865041 2118 16-17 -0.5774510024488606 2118 18-19 -0.5921790837223853 2118 20-21 -0.78855759189031 2118 22-23 -0.761800634413774 2118 24-25 -0.4946885198264326 2118 26-27 -0.44972773870377836 2118 28-29 -0.7499209652352405 2118 30-31 -0.4585647533095738 2118 32-33 -0.2933179241395223 2118 34-35 -0.39929231157270095 2118 36-37 -0.32063292581479885 2118 38-39 -0.11676736389419773 2118 40-41 -0.6073771834020292 2118 42-43 -0.457810905168202 2118 44-45 -0.6446329359159577 2118 46-47 -0.6381952977264476 2118 48-49 -0.476865776481759 2118 50-51 -0.22686711799371295 2118 52-53 -0.12607765749653055 2118 54-55 -0.49049909603849784 2118 56-57 -0.2782087363030712 2118 58-59 -0.29724395174358875 2118 60-61 -0.18953603222463045 2118 62-63 -0.1953018757165097 2118 64-65 -0.5859947269866339 2118 66-67 -0.4596366371637899 2118 68-69 -0.4601616141766627 2118 70-71 -0.2785682978680981 2118 72-73 -0.28447952695737655 2118 74-75 -0.3019778499994743 2118 76 -0.3076582984964418 2119 1 0.5445654101453137 2119 2 0.636412829219136 2119 3 -0.1491810108333702 2119 4 0.8351184621613505 2119 5 0.47831754830573203 2119 6 0.47584712894335723 2119 7 0.7108692467119013 2119 8 0.3063462457941597 2119 9 0.35820516906795064 2119 10-11 0.3670523552193643 2119 12-13 0.25979303328846726 2119 14-15 0.4962374944203347 2119 16-17 0.6974726539029348 2119 18-19 0.13804282181507688 2119 20-21 0.2089434260250016 2119 22-23 -0.06359216535840062 2119 24-25 0.31089983522242903 2119 26-27 0.1089652580388929 2119 28-29 0.20480725137714018 2119 30-31 0.3619971359412375 2119 32-33 0.08807967358034574 2119 34-35 -0.28050159496032023 2119 36-37 0.14502261164448527 2119 38-39 -0.17779647627205009 2119 40-41 0.16268592734715526 2119 42-43 0.25786499059726964 2119 44-45 0.35142773183648757 2119 46-47 -0.21408597362221116 2119 48-49 0.06345893850195239 2119 50-51 0.0714024259802244 2119 52-53 0.0432983604187811 2119 54-55 0.03787020363576943 2119 56-57 0.20166707802918182 2119 58-59 0.14399179744207657 2119 60-61 -0.2530030517686015 2119 62-63 0.45208717151475497 2119 64-65 0.24145844890912116 2119 66-67 0.24126930746161435 2119 68-69 -0.28324793339490384 2119 70-71 0.10265116467261493 2119 72-73 0.22952189812406232 2119 74-75 0.2561960099353797 2119 76 -0.5451990151088175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 19.0 13 286.0 14 1186.0 15 2072.0 16 2454.0 17 2840.0 18 2905.0 19 2454.0 20 1953.0 21 1439.0 22 1164.0 23 864.0 24 749.0 25 779.0 26 887.0 27 1040.0 28 1324.0 29 1485.0 30 1917.0 31 2475.0 32 3310.0 33 4375.0 34 6149.0 35 9683.0 36 18066.0 37 66141.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.497355417409324 16.99343563882972 9.793650103609675 30.715558840151285 2 23.678070976249472 18.614238722485474 29.18459911026098 28.523091191004074 3 20.461099276905912 21.33272471706589 26.16905041371415 32.03712559231405 4 17.340491819907548 17.305713747482212 28.52961207958382 36.824182353026416 5 17.362228115173384 29.689605703603878 31.642974104826905 21.305192076395834 6 33.363764146705506 29.525858945934587 16.778246315697952 20.332130591661958 7 30.725702444608675 26.67043429117941 18.162123780956108 24.441739483255805 8 26.54436377863757 29.267197032271152 19.86262661391992 24.325812575171355 9 25.148893622570966 16.76230636583634 18.848266168180963 39.240533843411725 10-11 27.835137445840395 21.651885985885897 23.50744105841267 27.005535509861033 12-13 26.455244968047648 19.45434653450999 23.70161862945413 30.38878986798823 14-15 25.66404382037126 20.119114898056775 21.664203219869872 32.5526380617021 16-17 26.786361199263865 22.541625005434074 21.946412786737962 28.7256010085641 18-19 25.881406773029603 21.481980611224625 22.882522569519917 29.754090046225855 20-21 25.551377356576676 21.96851135359156 23.41651088988393 29.06360039994783 22-23 27.197539451375906 21.680505441319248 22.341288817400628 28.780666289904218 24-25 26.354895738237044 22.578938978973756 21.744989783941225 29.32117549884798 26-27 25.19816255850686 22.98576997203263 23.11691228680317 28.699155182657332 28-29 26.036821284180323 22.338390644698517 23.96643916010955 27.65834891101161 30-31 26.15963135243229 21.56023127418163 23.88891304032807 28.391224333058005 32-33 24.815603761828168 22.798837832746454 24.201915692156096 28.183642713269286 34-35 25.272519789157172 22.665603999492816 24.126650605901425 27.935225605448583 36-37 25.603544465214682 21.440319378631774 24.279441811937573 28.67669434421597 38-39 24.08200379660624 21.46386703183643 26.318306307872884 28.135822863684446 40-41 24.016432639220973 20.93748641481546 26.72658638728282 28.319494558680752 42-43 22.82637047341651 20.79982321146517 26.769696706226725 29.604109608891594 44-45 21.991696735208453 20.467620165485663 28.702415626947207 28.838267472358677 46-47 21.743902969177935 21.22440551232448 28.833195670129985 28.198495848367607 48-49 21.18383109449492 21.964164094538393 28.429262849773217 28.422741961193466 50-51 20.815038618151256 23.213638800736135 27.31346635946036 28.65785622165225 52-53 19.737642916141375 23.500557898245155 27.495688968105608 29.266110217507862 54-55 19.95935312785289 22.849193583445636 27.45076729122288 29.74068599747859 56-57 20.158602501123042 23.23936008346737 27.13921372574592 29.46282368966367 58-59 20.595864307554088 22.88360938428321 26.58457592487936 29.935950383283338 60-61 20.560361691953226 22.16305119622078 26.833456505673176 30.443130606152817 62-63 20.68389630338072 22.52351142604588 26.354171195061515 30.43842107551189 64-65 21.123331739338347 22.78108652494602 24.910156646234547 31.185425089481082 66-67 21.17549884797635 23.628077497138054 23.742555318871453 31.453868336014146 68-69 21.141807590314308 24.452969902476486 23.276311785419292 31.12891072178991 70-71 21.270414003970497 25.603182193626917 23.073801967859264 30.052601834543317 72-73 21.047979249083454 25.953861090582386 23.071990609920444 29.926169050413716 74-75 20.872277529017953 27.50220985668536 23.393325508267036 28.23218710602965 76 21.33344926024142 27.61813676476981 22.61154342187251 28.43687055311626 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.5 15 1.5 16 3.0 17 6.0 18 6.0 19 12.0 20 23.5 21 29.0 22 38.5 23 63.5 24 91.0 25 103.0 26 162.5 27 264.5 28 382.0 29 457.0 30 539.5 31 744.0 32 1032.0 33 1198.0 34 1418.5 35 1932.5 36 2386.0 37 2546.0 38 2922.0 39 3826.5 40 4666.0 41 5280.0 42 5583.0 43 5708.5 44 6182.5 45 6642.5 46 6754.0 47 6723.0 48 6449.5 49 6055.0 50 5903.0 51 5467.0 52 4625.0 53 4030.5 54 3842.0 55 3779.0 56 3645.0 57 3718.0 58 3862.0 59 3933.5 60 4094.5 61 4288.5 62 4393.0 63 4464.5 64 4534.5 65 4520.0 66 4448.0 67 4389.0 68 4292.0 69 3960.5 70 3375.0 71 3024.0 72 2695.5 73 1996.5 74 1310.5 75 995.0 76 700.0 77 284.5 78 114.0 79 64.0 80 42.5 81 19.0 82 15.0 83 13.0 84 9.0 85 5.0 86 6.5 87 8.0 88 7.5 89 7.5 90 8.5 91 7.0 92 5.0 93 4.5 94 4.0 95 3.5 96 3.0 97 4.0 98 5.0 99 18.5 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.6227158776391485E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 138018.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.80446028778854 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2537931338222 49.52035241780058 2 14.669353240404307 19.30617745511455 3 4.97016141463522 9.81176368299787 4 2.016031358041003 5.306554217565824 5 0.9314923696901631 3.0648176324827197 6 0.6055801457796569 2.390992479241838 7 0.402986060646099 1.8562796157022998 8 0.30168901807931997 1.5881986407570028 9 0.19158353702847328 1.1346346128765812 >10 0.6573297218735549 6.020229245460737 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0014490863510556594 0.0 2 0.0 0.0 0.0 0.002898172702111319 0.0 3 0.0 0.0 0.0 0.010143604457389616 0.0 4 0.0 0.0 0.0 0.018113579388195744 0.0 5 0.0 0.0 0.0 0.019562665739251403 0.0 6 0.0 0.0 0.0 0.019562665739251403 0.0 7 0.0 0.0 0.0 0.019562665739251403 0.0 8 0.0 0.0 0.0 0.02173629526583489 0.0 9 0.0 0.0 0.0 0.026808097494529698 0.0 10 0.0 0.0 0.0 0.02970627019664102 0.0 11 0.0 0.0 0.0 0.02970627019664102 0.0 12 0.0 0.0 0.0 0.02970627019664102 0.0 13 0.0 0.0 0.0 0.031879899723224504 0.0 14 0.0 0.0 0.0 0.032604442898752334 0.0 15 0.0 0.0 0.0 0.03405352924980799 0.0 16 0.0 0.0 0.0 0.03405352924980799 0.0 17 0.0 0.0 0.0 0.03550261560086366 0.0 18 0.0 0.0 0.0 0.03840078830297498 0.0 19 0.0 0.0 0.0 0.042748047356141954 0.0 20 0.0 0.0 0.0 0.04781984958483676 0.0 21 0.0 0.0 0.0 0.04926893593589242 0.0 22 0.0 0.0 0.0 0.05071802228694808 0.0 23 0.0 0.0 0.0 0.052891651813531566 0.0 24 0.0 0.0 0.0 0.06158616991986553 0.0 25 0.0 0.0 0.0 0.07462794707936646 0.0 26 0.0 0.0 0.0 0.08911881058992305 0.0 27 0.0 0.0 0.0 0.1854830529351244 0.0 28 0.0 0.0 0.0 0.297787245141938 0.0 29 0.0 0.0 0.0 0.4506658551783101 0.0 30 0.0 0.0 0.0 0.5810836267733194 0.0 31 0.0 0.0 0.0 0.7622194206552768 0.0 32 0.0 0.0 0.0 0.9549479053456795 0.0 33 0.0 0.0 0.0 1.2034662145517252 0.0 34 0.0 0.0 0.0 1.5628396296135287 0.0 35 0.0 0.0 0.0 2.010607312089727 0.0 36 0.0 0.0 0.0 2.649654392905273 0.0 37 0.0 0.0 0.0 3.5756205712298397 0.0 38 0.0 0.0 0.0 4.627657262096248 0.0 39 0.0 0.0 0.0 5.74200466605805 0.0 40 0.0 0.0 0.0 6.832442145227434 0.0 41 0.0 0.0 0.0 8.127200799895666 0.0 42 0.0 0.0 0.0 9.546580880754684 0.0 43 0.0 0.0 0.0 10.942051036821285 0.0 44 0.0 0.0 0.0 12.197684360011014 0.0 45 0.0 0.0 0.0 13.39390514280746 0.0 46 0.0 0.0 0.0 14.591575011954962 0.0 47 0.0 0.0 0.0 15.739251401991044 0.0 48 0.0 0.0 0.0 16.782593574751118 0.0 49 0.0 0.0 0.0 17.73391876421916 0.0 50 0.0 0.0 0.0 18.698285730846703 0.0 51 0.0 0.0 0.0 19.740903360431247 0.0 52 0.0 0.0 0.0 20.89220246634497 0.0 53 0.0 0.0 0.0 22.048573374487386 0.0 54 0.0 0.0 0.0 23.103508238055905 0.0 55 0.0 0.0 0.0 24.07656972278978 0.0 56 0.0 0.0 0.0 24.949644249300817 0.0 57 0.0 0.0 0.0 25.775623469402543 0.0 58 0.0 0.0 0.0 26.69072150009419 0.0 59 0.0 0.0 0.0 27.530467040530944 0.0 60 0.0 7.245431755278297E-4 0.0 28.315871842803112 0.0 61 0.0 7.245431755278297E-4 0.0 29.082438522511556 0.0 62 0.0 7.245431755278297E-4 0.0 29.93595038328334 0.0 63 0.0 7.245431755278297E-4 0.0 30.80685128026779 0.0 64 0.0 7.245431755278297E-4 0.0 31.700937558869132 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGGTC 25 2.3836464E-4 55.999996 28 TTCTGAT 25 2.3836464E-4 55.999996 19 GACAGTG 20 0.006939146 52.5 25 GGAGCAA 20 0.006939146 52.5 26 CAAAAGA 20 0.006939146 52.5 1 GAACTAA 20 0.006939146 52.5 19 TGACATT 20 0.006939146 52.5 22 GAGCAAA 20 0.006939146 52.5 28 TTACCGG 20 0.006939146 52.5 42 ACGAATT 20 0.006939146 52.5 24 CATTACA 35 2.0778481E-5 50.000004 61 CGGTTGA 30 5.8613386E-4 46.666664 56 GACGCTT 30 5.8613386E-4 46.666664 14 TTTTCGG 30 5.8613386E-4 46.666664 26 TACATAT 40 4.5737175E-5 43.75 5 TTCCGAC 50 3.4585955E-6 42.0 19 ACGCTTC 50 3.4585955E-6 42.0 15 CTGAAGT 35 0.0012519333 40.000004 38 TTGGTAG 35 0.0012519333 40.000004 67 TAAGACC 35 0.0012519333 40.000004 60 >>END_MODULE