FastQCFastQC Report
Wed 25 May 2016
SRR1780226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138018
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT83716.065150922343462No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC22851.655581156081091No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG22371.620803083655755No Hit
CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG20181.4621281282151604RNA PCR Primer, Index 32 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5460.395600573838195No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT3280.23765016157312815RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT2730.1978002869190975No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT1790.12969322841948153No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1660.12027416713761972No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAGA150.002220746870.03
TCTTTAT850.061.7647061
CTTTATA950.055.263162
CTAAAAT200.00693914652.563
ATAAGTT200.00693914652.529
GCCTAAA200.00693914652.51
CATAAGT200.00693914652.528
CACCGGT200.00693914652.57
CGCAAAG200.00693914652.512
ACATAAG200.00693914652.527
TGAGAGC200.00693914652.549
TTTATAC1150.051.739133
ATCGTAT506.148366E-848.99999639
GCTTATA305.8613386E-446.6666643
ATATCGT551.301396E-744.54545637
TATCGTA551.301396E-744.54545638
ACTCGTA404.5737175E-543.7538
TCGATAT503.4585955E-642.034
CTCGATA503.4585955E-642.033
TTAATAC350.001251933340.0000042