FastQCFastQC Report
Wed 25 May 2016
SRR1780225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727629
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG301.3067384E-436.6374551
ACGTTTT300.005721531329.3563735
TACGTTT300.005721531329.3563734
GGTACAA300.00576594129.3099651
GGCTTAT300.00576594129.3099651
ATTAGAG554.99365E-627.9738483
GGGCTTA450.001404182924.424971
GATACCA500.00259349121.9794545
TCAGATG602.889456E-421.9794524
CTATACA654.956129E-420.2915131
AGTTGTT550.004447400620.02673520
GTTACAA550.00444917420.02535618
TTTTTGC550.004491906619.99231130
GAAAATT1101.2690361E-719.99231126
TAGAGCA550.00450622519.9813214
TTTAACG808.9282985E-519.26511834
TTAACGG808.9282985E-519.26511835
CATGTTT809.008856E-519.242630
TTTATAC1051.8067694E-618.839533
GTTTAAC951.6066762E-518.51155733
Produced by FastQC (version 0.11.5)