FastQCFastQC Report
Wed 25 May 2016
SRR1780225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727629
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT216812.9796778303228706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC61890.8505708266163113No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG56680.7789684028536521No Hit
CACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTG38480.5288409340474335Illumina PCR Primer Index 4 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC11960.16436947950122935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG5400.036.6694744
GTCTTCT6100.033.1828743
CTTTATA1200.032.997992
CCGTCTT6150.032.55533641
ATGCCGT29750.032.1705344
CAGATAT551.3731915E-731.9980516
TATGTTA551.3731915E-731.9980520
TTGGTAA353.2177617E-431.42665918
GTAAACA353.2177617E-431.42665921
TTCGTAG602.9424518E-729.3335635
ATGTTAA602.9442162E-729.33154721
TCTTTAT1400.028.3287661
CGTCTTC7250.027.91937842
TATGCCG34700.027.51795643
ATTGTTC407.031926E-427.4983258
TTTATAC1600.027.4983253
TTCAGAT655.928905E-727.07527514
AAACAGG508.340405E-526.39839223
GCCGTCT7850.026.06389840
AGTCTAT701.1311568E-625.14305540
Produced by FastQC (version 0.11.5)