FastQCFastQC Report
Wed 25 May 2016
SRR1780224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124721
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACTC150.00222024370.0000165
CACTGTT150.00222024370.0000145
GGAAGAC150.00222024370.0000158
ACAGAGC150.00222024370.0000143
CAAGACT207.9009755E-570.064
CTTGGGC252.3827513E-456.00000447
GCTAGTC252.3827513E-456.0000047
ATGGGAC200.006937585352.529
AGACTCC200.006937585352.566
ACAGTGC200.006937585352.53
TTTACCG200.006937585352.565
CCAGAGA200.006937585352.540
ACTAGAT200.006937585352.524
GATGGGA200.006937585352.528
TCAGTTA200.006937585352.533
GGCTAAA200.006937585352.566
GGTCCAA305.8591506E-446.66666811
AGTTGTT305.8591506E-446.66666820
AAGTTGT305.8591506E-446.66666819
TATTAAG305.8591506E-446.66666815
Produced by FastQC (version 0.11.5)