##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780224_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 124721 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.005388026074197 34.0 33.0 34.0 21.0 34.0 2 31.32878985896521 34.0 34.0 34.0 23.0 34.0 3 31.13551045934526 34.0 34.0 34.0 21.0 34.0 4 30.904178125576287 34.0 32.0 34.0 21.0 34.0 5 31.006165761980743 34.0 33.0 34.0 21.0 34.0 6 33.93036457372856 38.0 34.0 38.0 22.0 38.0 7 34.39775178197738 38.0 35.0 38.0 24.0 38.0 8 34.248065682603574 38.0 35.0 38.0 23.0 38.0 9 34.60814938943722 38.0 36.0 38.0 24.0 38.0 10-11 34.40635498432501 38.0 35.5 38.0 23.5 38.0 12-13 34.455925625997224 38.0 36.0 38.0 23.5 38.0 14-15 34.48784086080131 38.0 36.0 38.0 24.0 38.0 16-17 34.55740011706128 38.0 36.0 38.0 24.0 38.0 18-19 34.571351256003396 38.0 36.0 38.0 24.0 38.0 20-21 34.5794894203863 38.0 36.0 38.0 24.0 38.0 22-23 34.59514035326849 38.0 36.0 38.0 24.0 38.0 24-25 34.63200663881784 38.0 36.0 38.0 24.5 38.0 26-27 34.6721201722244 38.0 36.0 38.0 24.0 38.0 28-29 34.74494271213348 38.0 36.0 38.0 25.0 38.0 30-31 34.76593757266218 38.0 36.0 38.0 25.0 38.0 32-33 34.81530375798783 38.0 37.0 38.0 25.5 38.0 34-35 34.790223779475795 38.0 36.5 38.0 25.5 38.0 36-37 34.728217381194824 38.0 36.0 38.0 25.0 38.0 38-39 34.739955580856474 38.0 36.5 38.0 25.0 38.0 40-41 34.76904851628836 38.0 36.5 38.0 25.5 38.0 42-43 34.81665076450638 38.0 36.5 38.0 26.0 38.0 44-45 34.865191106549815 38.0 37.0 38.0 26.0 38.0 46-47 34.806147320820074 38.0 36.5 38.0 25.5 38.0 48-49 34.78431058121728 38.0 36.0 38.0 25.5 38.0 50-51 34.73745800626999 38.0 36.0 38.0 25.0 38.0 52-53 34.786094563064765 38.0 36.0 38.0 25.5 38.0 54-55 34.76428588609777 38.0 36.0 38.0 25.0 38.0 56-57 34.71518830028624 38.0 36.0 38.0 25.0 38.0 58-59 34.78575781143512 38.0 36.0 38.0 25.5 38.0 60-61 34.76754916974687 38.0 36.5 38.0 25.0 38.0 62-63 34.67643780919011 38.0 36.0 38.0 25.0 38.0 64-65 34.67144666896513 38.0 36.0 38.0 25.0 38.0 66-67 34.56294850105435 38.0 36.0 38.0 24.5 38.0 68-69 34.51901444023059 38.0 36.0 38.0 24.0 38.0 70-71 34.52451872579597 38.0 36.0 38.0 24.0 38.0 72-73 34.61108393935264 38.0 36.0 38.0 24.0 38.0 74-75 34.643111424699924 38.0 36.0 38.0 24.5 38.0 76 33.208048363948336 37.0 33.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02043700685268135 1101 2 0.09544457335869083 1101 3 0.41308861910216876 1101 4 0.21236185708491462 1101 5 -0.2986947462788727 1101 6 0.2016206520880175 1101 7 -0.445394296069729 1101 8 -0.3701621458346622 1101 9 -0.3767620513281713 1101 10-11 -0.4867881594275971 1101 12-13 -0.2317201397617552 1101 14-15 -0.7838769600693212 1101 16-17 -0.9203924058828861 1101 18-19 -0.30370676335108016 1101 20-21 -0.19707193516705956 1101 22-23 -0.11362127059251037 1101 24-25 -0.3269248620828833 1101 26-27 -0.6548598995981578 1101 28-29 -0.5510818596070521 1101 30-31 -0.472329339485853 1101 32-33 -0.7207586289825656 1101 34-35 -0.24148004158915626 1101 36-37 -0.3350662461049012 1101 38-39 -0.2784336492547297 1101 40-41 -0.4190147190509421 1101 42-43 -0.5969305478713025 1101 44-45 -0.47990171945682647 1101 46-47 -0.32120829783701765 1101 48-49 -0.4606782053996099 1101 50-51 -0.46950274076738907 1101 52-53 -0.19728844891398012 1101 54-55 -0.3402273789417549 1101 56-57 -0.40931337188209227 1101 58-59 -0.22411049622548518 1101 60-61 -0.21272354048458908 1101 62-63 -0.3818084916221238 1101 64-65 0.03023018687854062 1101 66-67 -0.2690051231045203 1101 68-69 -0.5376741519973223 1101 70-71 -0.6199116053456066 1101 72-73 -0.4815550065185761 1101 74-75 -0.4694237812414599 1101 76 -0.9914861950840645 1102 1 0.2549546380559349 1102 2 -0.11074994361258916 1102 3 0.2510651204076524 1102 4 0.0019832670065866864 1102 5 -0.5975981405347461 1102 6 0.392240756526661 1102 7 -0.020113617218555646 1102 8 -0.339333164111423 1102 9 0.4703658860086435 1102 10-11 -0.014510091542476289 1102 12-13 0.11921328060378045 1102 14-15 0.08608061173224968 1102 16-17 -0.04382256254084638 1102 18-19 0.16989636980818545 1102 20-21 -0.005684859449047508 1102 22-23 -0.2750050826029593 1102 24-25 -0.10187590646931 1102 26-27 -0.45335533040756104 1102 28-29 -0.26109491443733646 1102 30-31 -0.10558651964251453 1102 32-33 -0.11274949060136663 1102 34-35 0.03227648582598874 1102 36-37 -0.23031755158793032 1102 38-39 -0.21581941948971917 1102 40-41 -0.20168443184467577 1102 42-43 -0.4456642293334454 1102 44-45 -0.10123983956126636 1102 46-47 -0.3970405432678277 1102 48-49 -0.2558772915614824 1102 50-51 -0.2330373360676603 1102 52-53 0.08874941009385395 1102 54-55 -0.39867385413757717 1102 56-57 -0.41879117867060245 1102 58-59 0.0228118275343121 1102 60-61 0.0535068772699816 1102 62-63 -0.3341485699511111 1102 64-65 -0.007341614688037623 1102 66-67 0.06193156096858132 1102 68-69 -0.24895026165789602 1102 70-71 -0.10321774111584858 1102 72-73 -0.0731574869363314 1102 74-75 0.014753764450446738 1102 76 -0.01713893659842114 1103 1 -0.27447885948101103 1103 2 -0.6347770372086501 1103 3 -0.3166442391982578 1103 4 -0.15281968865843965 1103 5 -0.6065636577761282 1103 6 -0.87158929273442 1103 7 -0.6700274103219996 1103 8 -0.32385286854491824 1103 9 -0.4407178578337181 1103 10-11 -0.3836357073060199 1103 12-13 -0.31900100511051477 1103 14-15 -0.5608097823564222 1103 16-17 -0.17141492707287398 1103 18-19 -0.040645502112994336 1103 20-21 0.0892597218317448 1103 22-23 -0.3207686294009875 1103 24-25 -0.27955447789788224 1103 26-27 -0.37503636489032033 1103 28-29 -0.4023018109890586 1103 30-31 -0.4637761748149245 1103 32-33 -0.2460869290250116 1103 34-35 -0.37744642057795375 1103 36-37 -0.3103360244943332 1103 38-39 -0.8604314884552409 1103 40-41 -0.5353666978545277 1103 42-43 -0.4054425544565987 1103 44-45 0.0028118845766584855 1103 46-47 -0.47749005065699635 1103 48-49 -0.45225044427084526 1103 50-51 -0.7236346267080549 1103 52-53 -0.3454993583790582 1103 54-55 -0.28520094773363525 1103 56-57 -0.46765817374449625 1103 58-59 -0.5857903892144591 1103 60-61 -0.4242825315970151 1103 62-63 -0.7320734467983954 1103 64-65 -0.24753865902301442 1103 66-67 -0.3694662222826466 1103 68-69 -0.22040962126380492 1103 70-71 -0.30774360318481087 1103 72-73 -0.018680885951106063 1103 74-75 -0.3105664325938875 1103 76 -0.1978039612289635 1104 1 0.3031253697632543 1104 2 0.023579324680092384 1104 3 0.2628943887003352 1104 4 0.748934486518781 1104 5 -0.1936957015103573 1104 6 0.08120417116080603 1104 7 0.1389717486351003 1104 8 -0.2332965787455734 1104 9 -0.16499996764989078 1104 10-11 0.0970142987014313 1104 12-13 0.024030353774513458 1104 14-15 0.12983061173224542 1104 16-17 0.3834030472152463 1104 18-19 0.1703841746862338 1104 20-21 -0.18150803018075123 1104 22-23 -0.11841971674930107 1104 24-25 -0.08861371134735663 1104 26-27 0.08606540129976281 1104 28-29 0.4278075245870525 1104 30-31 0.19593787060139078 1104 32-33 0.3720675825693647 1104 34-35 0.2202947785089151 1104 36-37 0.009164155729145307 1104 38-39 -0.1004230780263029 1104 40-41 0.02849849498458923 1104 42-43 0.21055528286167657 1104 44-45 0.27699186775580387 1104 46-47 0.4799411640492508 1104 48-49 0.0387263669750979 1104 50-51 0.17150534685917052 1104 52-53 0.10783477594750934 1104 54-55 0.18900907269168954 1104 56-57 0.3082819920611044 1104 58-59 0.3411349982660141 1104 60-61 0.2043910236114428 1104 62-63 0.06521118614645616 1104 64-65 0.24339009262903488 1104 66-67 0.29994985365151194 1104 68-69 0.316446079805516 1104 70-71 0.3905322588841571 1104 72-73 0.1204095862344019 1104 74-75 0.26158303274312544 1104 76 0.28712935608450607 1105 1 -0.24608448174846487 1105 2 0.07352267252433009 1105 3 -0.6651573734798788 1105 4 -0.34465487480270696 1105 5 -0.06938298161054135 1105 6 -0.1754181676787141 1105 7 0.0989633998963626 1105 8 -0.680586220346143 1105 9 -0.28110110665639354 1105 10-11 0.036107267870725934 1105 12-13 -0.8670275506920646 1105 14-15 -0.3722476278765541 1105 16-17 -0.5320957899247176 1105 18-19 -0.314137860020665 1105 20-21 -0.6057551528475784 1105 22-23 -0.5289659627985586 1105 24-25 -0.5712065910658879 1105 26-27 -0.32390239642222696 1105 28-29 -0.311204938887208 1105 30-31 -0.33208407465473755 1105 32-33 -0.4365942338776563 1105 34-35 -0.3235211914356242 1105 36-37 -0.6000761090451405 1105 38-39 -0.3015620845263953 1105 40-41 -0.5316660944363605 1105 42-43 -0.28919723666840724 1105 44-45 -0.35217504738523076 1105 46-47 -0.15497147236318654 1105 48-49 -0.4443644553756698 1105 50-51 -0.47392670037083207 1105 52-53 -0.4887750397838957 1105 54-55 -0.48338045560456777 1105 56-57 -0.13519704664126664 1105 58-59 -0.40906775681776963 1105 60-61 -0.31134336722879397 1105 62-63 -0.3374156848655332 1105 64-65 -0.4096154533188425 1105 66-67 -0.17455071287005097 1105 68-69 -0.17772471153565306 1105 70-71 -0.17792739881755182 1105 72-73 -0.16624734023706367 1105 74-75 -0.15179880278672186 1105 76 -0.4083981053025809 1106 1 0.015381773734326032 1106 2 -0.061541400899020715 1106 3 0.21043697970413433 1106 4 0.1576365333382448 1106 5 0.1232058795657558 1106 6 0.283459349491487 1106 7 -0.2865583408366419 1106 8 -0.2315316565737362 1106 9 -0.2901868778104486 1106 10-11 -0.1465327046078002 1106 12-13 -0.1840404882404485 1106 14-15 -0.16212918726272818 1106 16-17 -0.35847708515391474 1106 18-19 -0.3058322734078942 1106 20-21 -0.07272631673547636 1106 22-23 -0.23013071074365854 1106 24-25 -0.40284952455976253 1106 26-27 -0.4833364861864524 1106 28-29 -0.32363260287954887 1106 30-31 -0.37072471059728684 1106 32-33 -0.2370082845712176 1106 34-35 -0.11902376543029192 1106 36-37 -0.17086403399999028 1106 38-39 -0.24914228381132375 1106 40-41 -0.2925763916436779 1106 42-43 -0.3466943800872144 1106 44-45 -0.20404134709895771 1106 46-47 -0.31440863372009176 1106 48-49 -0.3658207588981739 1106 50-51 -0.4234205018968069 1106 52-53 -0.21920285121267824 1106 54-55 -0.22075928127325284 1106 56-57 -0.27989670630879004 1106 58-59 -0.3309632980938275 1106 60-61 -0.07506724333303083 1106 62-63 -0.31064982623251325 1106 64-65 -0.29794462976341407 1106 66-67 -0.8592179365188528 1106 68-69 -0.3051374475875477 1106 70-71 -0.4310757813168493 1106 72-73 -0.2650481904539177 1106 74-75 -0.2670050295194031 1106 76 0.034242972949314776 1107 1 -0.4093310762297797 1107 2 -0.8853928007554472 1107 3 -0.6715879408168348 1107 4 -0.5827106105444351 1107 5 -0.7161695691061745 1107 6 -1.4908714883712975 1107 7 -0.7246544335450835 1107 8 -1.087751531458359 1107 9 -0.5877973792974771 1107 10-11 -0.4435407037873702 1107 12-13 -0.0013989642941822922 1107 14-15 -0.9053734699004039 1107 16-17 -0.45435827682656793 1107 18-19 -0.46750158937548747 1107 20-21 -0.32004710434700456 1107 22-23 -0.3414846744396911 1107 24-25 -0.3203708044284994 1107 26-27 -0.53174818755042 1107 28-29 -0.654105118518963 1107 30-31 -0.6543110094384303 1107 32-33 -0.302341327336066 1107 34-35 -0.2786163713168719 1107 36-37 -0.29626142913895137 1107 38-39 -0.6355898276529857 1107 40-41 -0.2123987175589619 1107 42-43 -0.3575009640273237 1107 44-45 -0.800627594663311 1107 46-47 -0.6073721759208865 1107 48-49 -0.49702525074516046 1107 50-51 -0.37484856055745297 1107 52-53 -0.32390365113063524 1107 54-55 -0.6046942623008462 1107 56-57 -0.6735870970379523 1107 58-59 -0.8221116418534464 1107 60-61 -0.6982533268116455 1107 62-63 -0.7642251005633511 1107 64-65 -0.6658620228513072 1107 66-67 -0.5311041533171306 1107 68-69 -0.609792098392596 1107 70-71 -0.499773863564819 1107 72-73 -0.6700707522424523 1107 74-75 -0.4433074600393496 1107 76 -0.0916287325167886 1108 1 -0.591097993523011 1108 2 -0.1400920488757471 1108 3 -0.5060401427502406 1108 4 -0.023016732993415445 1108 5 0.1492439647284094 1108 6 -0.4155224013680723 1108 7 -0.13182414353434524 1108 8 -0.24709632200615772 1108 9 -0.38489727188608924 1108 10-11 -0.4762206178582673 1108 12-13 -0.6161814562383299 1108 14-15 -0.8635904408993298 1108 16-17 -0.7315199309619018 1108 18-19 -0.8469457354549732 1108 20-21 -0.6262769647122042 1108 22-23 -0.6839524510240125 1108 24-25 -0.8120732748903663 1108 26-27 -0.5252632251443998 1108 28-29 -0.925305440753128 1108 30-31 -0.6996654670109308 1108 32-33 -0.6576179116540004 1108 34-35 -0.6181840404898011 1108 36-37 -1.108935972640559 1108 38-39 -0.642464156331819 1108 40-41 -0.4536581160552018 1108 42-43 -0.8367168609123965 1108 44-45 -1.146371418508643 1108 46-47 -0.6250010695836181 1108 48-49 -0.7573904494562171 1108 50-51 -1.030997862383444 1108 52-53 -0.8800663793798265 1108 54-55 -0.8299896436112633 1108 56-57 -0.5495806523548126 1108 58-59 -0.8667276461499043 1108 60-61 -0.8891904911510764 1108 62-63 -0.774872254161636 1108 64-65 -0.702341614688045 1108 66-67 -0.6543184390314138 1108 68-69 -0.36375289323684257 1108 70-71 -0.7528887937474238 1108 72-73 -1.0040127500942262 1108 74-75 -0.8902462355495473 1108 76 -0.6684547260721061 1109 1 0.24951386670607434 1109 2 -0.4638560042186519 1109 3 -0.14189631209923448 1109 4 -0.3035952453901096 1109 5 -0.5141821625733165 1109 6 0.12238538462583648 1109 7 -0.2469249119843937 1109 8 -0.23949156631528012 1109 9 -0.1865211663329518 1109 10-11 -0.16969604195569588 1109 12-13 -0.16689553482321884 1109 14-15 0.03217220952839028 1109 16-17 0.1750445173489581 1109 18-19 0.12777515768697612 1109 20-21 0.2019563251239589 1109 22-23 -0.04109323687293198 1109 24-25 -0.17920028663460386 1109 26-27 0.09071836105249531 1109 28-29 -0.1687119943271469 1109 30-31 0.1775746373822784 1109 32-33 0.08720230003224572 1109 34-35 -0.1496071229894369 1109 36-37 -0.11046562321327258 1109 38-39 0.2728685970392135 1109 40-41 -0.1424213464452322 1109 42-43 0.04184265771889528 1109 44-45 -0.054896864354660124 1109 46-47 -0.022608725086008974 1109 48-49 -0.19668652021162103 1109 50-51 -0.1264016611365264 1109 52-53 -0.19830981855628238 1109 54-55 0.1054170549533282 1109 56-57 -0.3101685891389252 1109 58-59 0.023924086487475904 1109 60-61 0.17970178085125355 1109 62-63 0.02748021792768185 1109 64-65 0.15185948724033693 1109 66-67 0.3376237097289163 1109 68-69 0.2422170937140038 1109 70-71 0.07235044070234409 1109 72-73 -0.18649426379583645 1109 74-75 0.017949356736949085 1109 76 -0.7628000936232127 1110 1 0.26709749519879367 1110 2 0.019607199244553897 1110 3 0.21035083469336868 1110 4 0.04412612414944306 1110 5 0.04240185946525088 1110 6 0.6625978993838046 1110 7 0.07095781135287638 1110 8 0.23102397874571778 1110 9 0.2489373145800755 1110 10-11 -0.03058152011390547 1110 12-13 0.15528470917520565 1110 14-15 0.03840204030367289 1110 16-17 0.2995702946019989 1110 18-19 0.31989636980819114 1110 20-21 -0.1072920023061883 1110 22-23 0.14356634596846618 1110 24-25 0.17758837924497328 1110 26-27 0.1257518124495789 1110 28-29 0.24247651413408988 1110 30-31 -0.027372233928225853 1110 32-33 0.14617908082720987 1110 34-35 -0.044687799888293966 1110 36-37 0.14414673412635892 1110 38-39 0.11257343765313976 1110 40-41 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16-17 0.3368307296402193 2116 18-19 0.574464271042757 2116 20-21 0.6833017660653553 2116 22-23 0.7435545881789309 2116 24-25 0.5305572211027041 2116 26-27 0.9007547518969687 2116 28-29 0.20036599091245222 2116 30-31 1.012880558546783 2116 32-33 0.579771085530318 2116 34-35 0.9681869796531473 2116 36-37 0.2501608434738003 2116 38-39 0.12839354347324416 2116 40-41 0.5823176257684963 2116 42-43 0.8192123138764273 2116 44-45 0.3408280616732995 2116 46-47 0.7127073991190045 2116 48-49 0.36772867551670885 2116 50-51 0.6305789190763775 2116 52-53 0.610961344250228 2116 54-55 0.531717092364481 2116 56-57 0.3578754879960613 2116 58-59 0.38188075757546613 2116 60-61 0.2220614040189517 2116 62-63 0.8219059567978633 2116 64-65 0.23418101905681965 2116 66-67 0.5805581041784578 2116 68-69 0.48168245439148194 2116 70-71 0.8185157979788045 2116 72-73 0.8034217311706655 2116 74-75 0.5713792788537404 2116 76 0.42041250373079464 2117 1 0.2839369389408901 2117 2 0.26489164930776354 2117 3 0.36261379297402385 2117 4 0.8057443289534874 2117 5 -0.3960052201807649 2117 6 0.05301509280985073 2117 7 1.0023863827814452 2117 8 0.8364854199593736 2117 9 -0.25043057416834813 2117 10-11 0.2181668996079651 2117 12-13 0.5375097407807701 2117 14-15 0.41345893031631675 2117 16-17 0.5790646056007418 2117 18-19 -0.05833371868738624 2117 20-21 -0.13788618688267462 2117 22-23 0.2927825280165095 2117 24-25 0.3803696687519249 2117 26-27 0.6146645810968678 2117 28-29 0.6949670324653212 2117 30-31 0.6812497635433274 2117 32-33 0.42669741205349965 2117 34-35 0.7958937424631571 2117 36-37 0.7393174041642823 2117 38-39 0.8424217309527577 2117 40-41 0.6269660106331969 2117 42-43 0.6226101069497432 2117 44-45 0.4447557356599674 2117 46-47 0.6260236160242059 2117 48-49 0.8795098765801086 2117 50-51 0.673124168357127 2117 52-53 0.6765591446071255 2117 54-55 0.3600429600217083 2117 56-57 0.5274920071701032 2117 58-59 0.6475131549679318 2117 60-61 0.7083409480664429 2117 62-63 0.4342916955356202 2117 64-65 0.3779681198252334 2117 66-67 0.5808585521190324 2117 68-69 0.9986714197580326 2117 70-71 0.7666384535744228 2117 72-73 -1.0572614692372113 2117 74-75 0.2528953573707966 2117 76 0.13839203685290613 2118 1 0.4374173246230981 2118 2 0.5718744344968627 2118 3 0.8360651204076532 2118 4 0.18618724710608703 2118 5 1.1265809639428639 2118 6 1.2188452341386053 2118 7 0.975023198701841 2118 8 1.0962265373811135 2118 9 1.0069330501877474 2118 10-11 -0.004447902487747513 2118 12-13 0.8721859174196993 2118 14-15 0.8927659351153281 2118 16-17 0.7279000742750625 2118 18-19 0.9742247280171483 2118 20-21 1.0121820559738381 2118 22-23 0.7303183004816205 2118 24-25 1.1849089194013303 2118 26-27 0.9295613362591126 2118 28-29 1.1316662795925154 2118 30-31 0.992734375879877 2118 32-33 1.2316659322842014 2118 34-35 0.723116038064795 2118 36-37 0.9695269757752527 2118 38-39 0.8669231178237098 2118 40-41 0.8472583542249765 2118 42-43 0.9600820393232681 2118 44-45 0.45203130471733743 2118 46-47 0.7039855761351532 2118 48-49 0.7945144994832916 2118 50-51 1.078585798260704 2118 52-53 1.0988613503923546 2118 54-55 0.8032913199917786 2118 56-57 1.1290197666030295 2118 58-59 0.8357533698228679 2118 60-61 0.8064235439366456 2118 62-63 1.1450740668648152 2118 64-65 0.7455439574512681 2118 66-67 0.9328706156949522 2118 68-69 0.8875236189391202 2118 70-71 1.2139153434612737 2118 72-73 0.5372218662975001 2118 74-75 0.7775398341021926 2118 76 0.6882093221080439 2119 1 1.3049546380559356 2119 2 0.8571667230540747 2119 3 0.2683567870743211 2119 4 0.9592749336732531 2119 5 0.5509435261319204 2119 6 1.0189074231933262 2119 7 1.2628030494481095 2119 8 1.0206668358885764 2119 9 1.2318242193419806 2119 10-11 0.9090315751241889 2119 12-13 0.8937966139371127 2119 14-15 0.7454556117322468 2119 16-17 1.007323270792476 2119 18-19 1.1602088698081872 2119 20-21 1.0100443072176262 2119 22-23 0.6257240840637124 2119 24-25 0.8778115935306872 2119 26-27 0.7628946695924412 2119 28-29 0.9260925855626638 2119 30-31 0.716809313690824 2119 32-33 0.8855838427319611 2119 34-35 0.9300889858259893 2119 36-37 0.9879116150787439 2119 38-39 0.9466805805102823 2119 40-41 1.1514405681553228 2119 42-43 0.7108982706665543 2119 44-45 0.6921976604387297 2119 46-47 0.2165011233988423 2119 48-49 0.47443520843851417 2119 50-51 0.5073793305990151 2119 52-53 0.9676035767605171 2119 54-55 0.8916386458624217 2119 56-57 0.3422504879960684 2119 58-59 1.1323951608676381 2119 60-61 0.6710068772699813 2119 62-63 0.7109555967155643 2119 64-65 0.5934917186452964 2119 66-67 0.37068156096858473 2119 68-69 -0.05728359499122604 2119 70-71 0.3098030922174928 2119 72-73 0.639134179730334 2119 74-75 0.7045454311171184 2119 76 0.27348606340157744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 8.0 13 178.0 14 810.0 15 1395.0 16 1779.0 17 1785.0 18 1667.0 19 1307.0 20 1036.0 21 745.0 22 610.0 23 513.0 24 535.0 25 568.0 26 675.0 27 746.0 28 952.0 29 1291.0 30 1584.0 31 2200.0 32 2829.0 33 4111.0 34 5964.0 35 9926.0 36 20160.0 37 61347.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.98575219890796 22.090906904210197 14.61421893666664 26.309121960215197 2 18.78031766903729 22.855012387649232 33.6863880180563 24.678281925257174 3 17.860665004289576 25.627600804996753 29.07850321918522 27.43323097152845 4 13.847708084444479 19.6053591616488 34.629292581040886 31.917640172865834 5 13.875770720247594 33.95578932176618 35.40141596042366 16.76702399756256 6 26.98743595705615 35.12399676077004 20.82808829307013 17.060478989103682 7 25.981190016115967 29.916373345306724 23.188556858909084 20.91387977966822 8 23.179737173370967 33.07782971592595 22.555143079353115 21.187290031349974 9 23.51568701341394 17.94485291169891 23.255105395242182 35.28435467964497 10-11 25.026659504012954 25.007416553747962 26.745295499555006 23.22062844268407 12-13 24.113822050817426 22.89550276216515 26.09183698013967 26.898838206877752 14-15 22.959645929715126 23.92339702215345 24.146695424186785 28.970261623944644 16-17 23.57261407461454 26.023684864617824 25.225102428620684 25.178598632146954 18-19 22.357501944339766 25.58711043048083 26.711620336591274 25.343767288588126 20-21 22.292556987195418 26.0878280321678 26.271437849279593 25.34817713135719 22-23 23.72214783396541 25.700563658084846 25.833259835953847 24.744028671995892 24-25 23.226240969844696 25.978784647332848 25.570673743796153 25.22430063902631 26-27 22.27171045774168 26.268631585699282 26.298698695488333 25.16095926107071 28-29 22.840981069747677 25.628402594591126 27.69220901051146 23.838407325149735 30-31 22.68503299364181 25.458022305786514 27.14178045397327 24.71516424659841 32-33 22.24485050633013 26.23094747476367 26.82587535378966 24.69832666511654 34-35 22.668997201754316 25.787156934277306 27.079641760409235 24.464204103559144 36-37 23.05586068104008 25.136504678442286 26.83870398729965 24.96893065321798 38-39 21.9169185622309 25.820030307646668 27.90668772700668 24.356363403115754 40-41 22.225206661267947 24.722380352947777 28.2442411462384 24.808171839545867 42-43 21.531658662133882 24.863896216354906 27.955596892263536 25.64884822924768 44-45 21.035751798013166 24.641800498713128 28.745359642722555 25.57708806055115 46-47 21.16403813311311 24.9841646555111 29.00273410251682 24.849063108858974 48-49 20.7912059717289 25.1232751501351 29.20278060631329 24.882738271822706 50-51 20.875794773935425 25.93348353525068 28.004906952317572 25.185814738496326 52-53 20.13814834711075 25.728626293887956 28.500813816438292 25.632411542563 54-55 19.94251168608334 25.75829250887982 28.39457669518365 25.904619109853193 56-57 19.945718844460835 25.757891614082634 28.38896416802303 25.9074253734335 58-59 20.383896857786578 25.455616937003388 27.939561100376036 26.22092510483399 60-61 20.35503243238909 24.974543180378607 28.125977181068144 26.544447206164158 62-63 20.013069170388306 25.097617883115113 28.182102452674368 26.70721049382221 64-65 20.691383167229255 25.76991845799825 26.799416297175295 26.7392820775972 66-67 20.394320122513452 25.70336992166516 26.384891076883605 27.51741887893779 68-69 20.38830670055564 26.422174293022028 26.09865219169186 27.09086681473048 70-71 20.954053263096284 26.562593960094773 25.828151747306975 26.655201029501967 72-73 20.919091412031655 26.366449916212986 25.970766751389103 26.74369192036626 74-75 20.549065514227756 27.67136248105772 25.86893947290352 25.910632531811 76 20.67895542851645 27.41238444207471 25.911434321405373 25.997225808003467 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 3.5 15 5.0 16 8.0 17 12.0 18 14.5 19 19.5 20 26.0 21 30.0 22 50.5 23 94.0 24 133.5 25 150.0 26 239.5 27 410.5 28 603.0 29 714.0 30 849.5 31 1177.0 32 1650.0 33 1931.0 34 2144.0 35 2790.0 36 3419.5 37 3616.0 38 4106.5 39 5321.5 40 6541.5 41 7169.0 42 7301.0 43 7529.5 44 8035.5 45 8406.0 46 8499.0 47 8485.5 48 8087.5 49 7141.0 50 6579.0 51 6120.5 52 4979.5 53 3911.5 54 3526.0 55 3470.0 56 2987.5 57 2365.5 58 2170.0 59 1991.0 60 1775.0 61 1624.0 62 1510.0 63 1499.0 64 1466.0 65 1427.5 66 1396.0 67 1381.0 68 1302.0 69 1163.5 70 987.0 71 870.0 72 728.0 73 487.0 74 292.5 75 197.0 76 144.0 77 54.5 78 13.5 79 9.0 80 6.5 81 5.5 82 7.5 83 8.0 84 7.0 85 5.0 86 3.5 87 3.0 88 1.5 89 1.5 90 2.0 91 1.0 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 1.5 98 2.0 99 13.5 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 4.008947971873221E-4 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 124721.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.928873245083025 #Duplication Level Percentage of deduplicated Percentage of total 1 72.10755836836222 36.72356700154745 2 12.09401911239157 12.318695327972033 3 5.074072324816196 7.752503588008435 4 2.726743179206222 5.554798309827535 5 1.7664006045435225 4.498039624441754 6 1.3035469701978937 3.9832907048532324 7 0.9792345597380311 3.4909918939072004 8 0.7525307388340496 3.0660434088886395 9 0.636030164202837 2.9153069651462067 >10 2.556715313528236 19.600548424082554 >50 0.003148664179221965 0.0962147513249573 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004810737566247865 0.0 2 0.0 0.0 0.0 0.008819685538121087 0.0 3 0.0 0.0 0.0 0.008819685538121087 0.0 4 0.0 0.0 0.0 0.03046800458623648 0.0 5 0.0 0.0 0.0 0.03126979418061113 0.0 6 0.0 0.0 0.0 0.03126979418061113 0.0 7 0.0 0.0 0.0 0.03126979418061113 0.0 8 0.0 0.0 0.0 0.03126979418061113 0.0 9 0.0 0.0 0.0 0.035278742152484346 0.0 10 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 11 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 12 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 13 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 14 0.0 8.017895943746442E-4 0.0 0.03848590052998292 0.0 15 0.0 8.017895943746442E-4 0.0 0.03848590052998292 0.0 16 0.0 8.017895943746442E-4 0.0 0.03848590052998292 0.0 17 0.0 8.017895943746442E-4 0.0 0.039287690124357566 0.0 18 0.0 8.017895943746442E-4 0.0 0.04249484850185614 0.0 19 0.0 8.017895943746442E-4 0.0 0.04810737566247865 0.0 20 0.0 8.017895943746442E-4 0.0 0.05051274444560259 0.0 21 0.0 8.017895943746442E-4 0.0 0.052116323634351876 0.0 22 0.0 8.017895943746442E-4 0.0 0.05772885079497438 0.0 23 0.0 8.017895943746442E-4 0.0 0.05853064038934903 0.0 24 0.0 8.017895943746442E-4 0.0 0.06093600917247296 0.0 25 0.0 8.017895943746442E-4 0.0 0.06414316754997154 0.0 26 0.0 8.017895943746442E-4 0.0 0.07055748430496869 0.0 27 0.0 8.017895943746442E-4 0.0 0.12988991428869237 0.0 28 0.0 8.017895943746442E-4 0.0 0.1796008691399203 0.0 29 0.0 8.017895943746442E-4 0.0 0.22369929683052572 0.0 30 0.0 8.017895943746442E-4 0.0 0.2918514123523705 0.0 31 0.0 8.017895943746442E-4 0.0 0.35278742152484344 0.0 32 0.0 8.017895943746442E-4 0.0 0.4257502746129361 0.0 33 0.0 8.017895943746442E-4 0.0 0.5147489195885215 0.0 34 0.0 8.017895943746442E-4 0.0 0.6550620986040843 0.0 35 0.0 8.017895943746442E-4 0.0 0.8547077076033707 0.0 36 0.0 8.017895943746442E-4 0.0 1.1521716471163637 0.0 37 0.0 8.017895943746442E-4 0.0 1.5747147633518013 0.0 38 0.0 8.017895943746442E-4 0.0 2.044563465655343 0.0 39 0.0 8.017895943746442E-4 0.0 2.5520962788944925 0.0 40 0.0 8.017895943746442E-4 0.0 3.118961522117366 0.0 41 0.0 8.017895943746442E-4 0.0 3.768411093560828 0.0 42 0.0 8.017895943746442E-4 0.0 4.5148772059236215 0.0 43 0.0 8.017895943746442E-4 0.0 5.2797844789570325 0.0 44 0.0 8.017895943746442E-4 0.0 6.001395113894212 0.0 45 0.0 8.017895943746442E-4 0.0 6.791959653947611 0.0 46 0.0 8.017895943746442E-4 0.0 7.565686612519142 0.0 47 0.0 8.017895943746442E-4 0.0 8.249613136520715 0.0 48 0.0 8.017895943746442E-4 0.0 8.879819757699185 0.0 49 0.0 8.017895943746442E-4 0.0 9.538089014680768 0.0 50 0.0 8.017895943746442E-4 0.0 10.177917110991734 0.0 51 0.0 8.017895943746442E-4 0.0 10.853825739049558 0.0 52 0.0 8.017895943746442E-4 0.0 11.600291851412353 0.0 53 0.0 8.017895943746442E-4 0.0 12.322704275943908 0.0 54 0.0 8.017895943746442E-4 0.0 13.062756071551703 0.0 55 0.0 8.017895943746442E-4 0.0 13.719421749344537 0.0 56 0.0 8.017895943746442E-4 0.0 14.40014111496861 0.0 57 0.0 8.017895943746442E-4 0.0 14.924511509689626 0.0 58 0.0 8.017895943746442E-4 0.0 15.490574963318126 0.0 59 0.0 8.017895943746442E-4 0.0 16.06144915451287 0.0 60 0.0 8.017895943746442E-4 0.0 16.676421773398225 0.0 61 0.0 8.017895943746442E-4 0.0 17.26974607323546 0.0 62 0.0 8.017895943746442E-4 0.0 17.879907954554564 0.0 63 0.0 8.017895943746442E-4 0.0 18.536573632347398 0.0 64 0.0 8.017895943746442E-4 0.0 19.165176674337122 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGACTC 15 0.002220243 70.00001 65 CACTGTT 15 0.002220243 70.00001 45 GGAAGAC 15 0.002220243 70.00001 58 ACAGAGC 15 0.002220243 70.00001 43 CAAGACT 20 7.9009755E-5 70.0 64 CTTGGGC 25 2.3827513E-4 56.000004 47 GCTAGTC 25 2.3827513E-4 56.000004 7 ATGGGAC 20 0.0069375853 52.5 29 AGACTCC 20 0.0069375853 52.5 66 ACAGTGC 20 0.0069375853 52.5 3 TTTACCG 20 0.0069375853 52.5 65 CCAGAGA 20 0.0069375853 52.5 40 ACTAGAT 20 0.0069375853 52.5 24 GATGGGA 20 0.0069375853 52.5 28 TCAGTTA 20 0.0069375853 52.5 33 GGCTAAA 20 0.0069375853 52.5 66 GGTCCAA 30 5.8591506E-4 46.666668 11 AGTTGTT 30 5.8591506E-4 46.666668 20 AAGTTGT 30 5.8591506E-4 46.666668 19 TATTAAG 30 5.8591506E-4 46.666668 15 >>END_MODULE