FastQCFastQC Report
Wed 25 May 2016
SRR1780224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124721
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT39543.170276056157343No Hit
CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG20911.676542041837381Illumina PCR Primer Index 8 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC10420.8354647573383793No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG10080.8082039111296414No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3250.26058161817175934No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT2080.166772235629926RNA PCR Primer, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAC207.9009755E-570.043
TTTCGCC200.006937585352.533
TTATGCA200.006937585352.550
ACGATGC200.006937585352.58
ATGGTCA200.006937585352.514
CCGAAGA200.006937585352.529
GGCGCAT200.006937585352.510
TAAGCCA200.006937585352.543
TGGTCAT200.006937585352.515
TACTTAT305.8591506E-446.66666847
TATGACA305.8591506E-446.66666853
ACTAAGC404.5711666E-543.7541
CTAGAAC350.001251469140.064
ACACAGT350.001251469140.034
TTACTTA350.001251469140.046
TACATAC350.001251469140.043
GGATATT459.154789E-538.888891
TCCATAC400.002411199735.064
TTTACAC400.002411199735.03
CTTATAT400.002411199735.049