##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780224_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 124721 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.56922250463033 34.0 34.0 34.0 34.0 34.0 2 33.57653482573103 34.0 34.0 34.0 34.0 34.0 3 33.64336398842216 34.0 34.0 34.0 34.0 34.0 4 33.69223306419929 34.0 34.0 34.0 34.0 34.0 5 33.68272383961001 34.0 34.0 34.0 34.0 34.0 6 37.31678706873742 38.0 38.0 38.0 36.0 38.0 7 37.35811130443149 38.0 38.0 38.0 37.0 38.0 8 37.198555175150936 38.0 38.0 38.0 36.0 38.0 9 37.22259282719029 38.0 38.0 38.0 36.0 38.0 10-11 37.152977445658706 38.0 38.0 38.0 36.0 38.0 12-13 37.15848574017207 38.0 38.0 38.0 36.0 38.0 14-15 37.0826965787638 38.0 38.0 38.0 36.0 38.0 16-17 37.11656417123018 38.0 38.0 38.0 36.0 38.0 18-19 37.096431234515435 38.0 38.0 38.0 35.5 38.0 20-21 37.12412504710514 38.0 38.0 38.0 36.0 38.0 22-23 37.080672060038005 38.0 38.0 38.0 36.0 38.0 24-25 37.11481626991445 38.0 38.0 38.0 36.0 38.0 26-27 36.979943233296716 38.0 38.0 38.0 35.5 38.0 28-29 36.93452987067134 38.0 37.5 38.0 35.0 38.0 30-31 36.92105980548585 38.0 37.0 38.0 35.0 38.0 32-33 36.873188957753705 38.0 37.0 38.0 35.0 38.0 34-35 36.88726838303093 38.0 37.0 38.0 35.0 38.0 36-37 36.91618893370002 38.0 37.0 38.0 35.0 38.0 38-39 36.57426576117895 38.0 37.0 38.0 34.0 38.0 40-41 36.71952598199181 38.0 37.0 38.0 34.5 38.0 42-43 36.831175183008476 38.0 37.0 38.0 35.0 38.0 44-45 36.88373249091973 38.0 37.0 38.0 35.0 38.0 46-47 36.8198539139359 38.0 37.0 38.0 34.5 38.0 48-49 36.82644061545369 38.0 37.0 38.0 35.0 38.0 50-51 36.666688047722516 38.0 37.0 38.0 34.0 38.0 52-53 36.721494375446 38.0 37.0 38.0 34.0 38.0 54-55 36.656870134139396 38.0 37.0 38.0 34.0 38.0 56-57 36.71602216146439 38.0 37.0 38.0 34.0 38.0 58-59 36.63118881343158 38.0 37.0 38.0 34.0 38.0 60-61 36.63735056646435 38.0 37.0 38.0 34.0 38.0 62-63 36.56826035711708 38.0 37.0 38.0 34.0 38.0 64-65 36.49207831880758 38.0 37.0 38.0 34.0 38.0 66-67 36.15871425020646 38.0 37.0 38.0 33.5 38.0 68-69 36.12671883644294 38.0 37.0 38.0 34.0 38.0 70-71 35.72606056718596 38.0 37.0 38.0 32.0 38.0 72-73 35.194698567201996 38.0 37.0 38.0 26.5 38.0 74-75 34.99358167429703 38.0 37.0 38.0 25.0 38.0 76 34.16374948885913 38.0 34.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0816649123390718 1101 2 0.011760925344411532 1101 3 -0.07143475388392062 1101 4 0.16728193685059978 1101 5 0.12389631557559255 1101 6 0.0675909030315438 1101 7 0.17945365094873011 1101 8 0.049940818312471436 1101 9 0.012242625589948375 1101 10-11 0.16896211197645528 1101 12-13 0.13059800787100784 1101 14-15 0.4421830917006915 1101 16-17 0.3836059316494129 1101 18-19 0.27822122397680005 1101 20-21 0.24628063862918026 1101 22-23 0.08630058098238891 1101 24-25 0.06306151201060572 1101 26-27 -0.019652170440245698 1101 28-29 -0.07002322162745145 1101 30-31 -0.028746234751352517 1101 32-33 -0.033193514903793186 1101 34-35 0.08859657741450633 1101 36-37 0.29726758237375606 1101 38-39 0.06717249588769647 1101 40-41 0.14735411822083222 1101 42-43 0.29158666947265743 1101 44-45 0.033734117853576606 1101 46-47 0.03780287698927509 1101 48-49 0.25127170232732965 1101 50-51 0.12684051645715044 1101 52-53 0.13186926734524462 1101 54-55 0.2119956355491368 1101 56-57 0.16746279668863195 1101 58-59 0.23627148014711707 1101 60-61 0.01091340513539052 1101 62-63 0.17064963115282694 1101 64-65 0.18923406383968455 1101 66-67 0.13750599931971408 1101 68-69 -0.5003340904182636 1101 70-71 0.2392583470492653 1101 72-73 0.17705287455714114 1101 74-75 0.007912434102991028 1101 76 -0.725143847028491 1102 1 0.1558611973215065 1102 2 0.10201549453501002 1102 3 0.18776237405341334 1102 4 -0.0041149299901306335 1102 5 -6.728750249251902E-4 1102 6 0.27055434950673884 1102 7 0.11814816792000471 1102 8 0.006566144683226582 1102 9 -0.08835789660326299 1102 10-11 0.07006198142815379 1102 12-13 0.024305579672571298 1102 14-15 0.12323726924638123 1102 16-17 -0.013009603598625574 1102 18-19 0.11948754251465488 1102 20-21 0.2479092287075062 1102 22-23 0.16644418411554796 1102 24-25 0.020760598172486766 1102 26-27 0.37417615854147357 1102 28-29 0.1936321308529685 1102 30-31 0.16705010989616653 1102 32-33 0.16965570180638423 1102 34-35 0.07750976279309185 1102 36-37 0.17124930561136154 1102 38-39 -0.07424068949089246 1102 40-41 0.1425401495524241 1102 42-43 0.034896068950466486 1102 44-45 0.011116624380989037 1102 46-47 -0.06837205773657473 1102 48-49 0.03585721146571785 1102 50-51 0.09768581671824705 1102 52-53 -0.06814378748504879 1102 54-55 -0.16204483442475492 1102 56-57 -0.0051579604915232835 1102 58-59 0.35609523993824155 1102 60-61 0.2639192798090235 1102 62-63 -0.04514671349468813 1102 64-65 -0.1425120197112335 1102 66-67 0.21129842751814465 1102 68-69 0.42533823334153453 1102 70-71 -0.11513460334237635 1102 72-73 -0.22798302622615552 1102 74-75 -0.03597790532259637 1102 76 0.08821124435532113 1103 1 -0.04917574849130801 1103 2 0.06252042064338781 1103 3 0.1438091720829746 1103 4 0.1212298975960735 1103 5 0.24863746980265944 1103 6 0.14673661551658768 1103 7 0.01804964575251944 1103 8 0.12411540576697178 1103 9 0.1664696896036304 1103 10-11 0.006779961723722749 1103 12-13 0.17755681119967193 1103 14-15 0.2366917027439186 1103 16-17 0.14427487915999393 1103 18-19 0.24803434054420848 1103 20-21 0.1323981449636662 1103 22-23 0.29085305111062354 1103 24-25 -0.12763225897037955 1103 26-27 0.28594339992078943 1103 28-29 0.17427252494163525 1103 30-31 0.04591710497005863 1103 32-33 0.16366432249604657 1103 34-35 0.05150606821181469 1103 36-37 0.10900792630101108 1103 38-39 -0.0755399505746368 1103 40-41 0.31356847467557714 1103 42-43 0.28766700491105723 1103 44-45 0.23728657511991003 1103 46-47 0.26051956787919295 1103 48-49 0.2281170636824612 1103 50-51 0.17744566893499325 1103 52-53 0.08606803517505313 1103 54-55 0.2126226532599773 1103 56-57 -0.13304589152600244 1103 58-59 0.23248686555400155 1103 60-61 0.0898983438484322 1103 62-63 0.21790747369743002 1103 64-65 0.4906591625547776 1103 66-67 0.2826654225920393 1103 68-69 0.335030351568129 1103 70-71 -0.015220810238936622 1103 72-73 -0.179196080413341 1103 74-75 -0.18395820088910142 1103 76 -0.16358678520132486 1104 1 0.2898246119556518 1104 2 0.07585695794964664 1104 3 0.20465261795585832 1104 4 0.10600702122938088 1104 5 0.12298566156043478 1104 6 0.37921288609210535 1104 7 0.1244896313346402 1104 8 0.069675900780787 1104 9 0.12652015217722123 1104 10-11 0.26521442045253707 1104 12-13 0.21625679918477658 1104 14-15 0.10113361070979465 1104 16-17 0.11445990859649413 1104 18-19 0.2442436400756378 1104 20-21 0.19995191163433645 1104 22-23 0.33802954996921386 1104 24-25 0.29170572012370144 1104 26-27 0.3338407926878233 1104 28-29 0.3726565210968644 1104 30-31 0.32851352453030813 1104 32-33 0.26505204326979737 1104 34-35 0.32565000669553257 1104 36-37 0.20673711048940646 1104 38-39 0.1191434568505656 1104 40-41 0.204887710528034 1104 42-43 0.3589814348041287 1104 44-45 0.4071532097468449 1104 46-47 0.11711574714148298 1104 48-49 0.29991208951449266 1104 50-51 0.06753337769385581 1104 52-53 0.09002694422226654 1104 54-55 0.14655272655085838 1104 56-57 -0.12110308244274393 1104 58-59 0.26713182530409796 1104 60-61 0.2783400115163417 1104 62-63 0.47924353040775003 1104 64-65 0.23825017541072668 1104 66-67 0.12135940312790439 1104 68-69 0.21613091626836223 1104 70-71 0.03187759177956906 1104 72-73 0.5293645347494547 1104 74-75 0.3486562410188654 1104 76 0.8441868541114204 1105 1 -0.021076455490231183 1105 2 0.1361169615276765 1105 3 0.1382085843223635 1105 4 0.01993152477758997 1105 5 0.25685768732225966 1105 6 0.08741254021578015 1105 7 0.25761027060949004 1105 8 -0.02500475996225049 1105 9 0.13704552637961598 1105 10-11 0.42143423081689946 1105 12-13 0.37230068969702756 1105 14-15 0.2958381249921018 1105 16-17 0.34832597097350515 1105 18-19 0.3854655376246825 1105 20-21 0.11870079349969131 1105 22-23 0.1945982183453765 1105 24-25 0.17266463240230934 1105 26-27 0.2758051316466137 1105 28-29 0.32556367119526186 1105 30-31 0.24218458422379285 1105 32-33 0.2163580245447747 1105 34-35 0.15614973834321688 1105 36-37 0.20082143275072184 1105 38-39 0.0570765476729207 1105 40-41 0.06407132314655684 1105 42-43 0.4155501031802942 1105 44-45 -0.08976968368746441 1105 46-47 -4.1362252874677097E-4 1105 48-49 0.17298740706473126 1105 50-51 0.053245107671791914 1105 52-53 0.21037699491103723 1105 54-55 0.09571188929162844 1105 56-57 0.3973267583348772 1105 58-59 0.30835074116074424 1105 60-61 0.4577365169728367 1105 62-63 0.10020780973269439 1105 64-65 0.024605950948917155 1105 66-67 0.21731920991422982 1105 68-69 0.6667868885982529 1105 70-71 0.14855121572852426 1105 72-73 0.1618977806198174 1105 74-75 -0.14935198845218167 1105 76 0.33553398274163015 1106 1 0.1393159711908467 1106 2 -0.04535887732428279 1106 3 -0.006672299313414953 1106 4 0.1324931102108735 1106 5 -0.05529599060281498 1106 6 0.16525284196904977 1106 7 -0.015319168763411994 1106 8 0.23627719995960916 1106 9 0.23118984209020255 1106 10-11 0.07231700655377438 1106 12-13 0.1015920118333753 1106 14-15 0.18432395266346902 1106 16-17 0.1969087381099115 1106 18-19 -0.09214562331448661 1106 20-21 0.013317520164797259 1106 22-23 0.08561503838690498 1106 24-25 0.15915883937850595 1106 26-27 0.03525027914449197 1106 28-29 -0.053553798795277885 1106 30-31 -0.06269863382242846 1106 32-33 -0.08291339367099937 1106 34-35 -0.033413604041079736 1106 36-37 0.10112367747066031 1106 38-39 -0.07338013672707433 1106 40-41 0.20080019980368036 1106 42-43 0.16711968704091618 1106 44-45 0.22399350880310465 1106 46-47 0.15522090708754632 1106 48-49 0.1819564576968702 1106 50-51 0.32852124385392045 1106 52-53 0.18595168990189137 1106 54-55 0.21432451230892013 1106 56-57 0.23933324553862434 1106 58-59 0.12341936054125568 1106 60-61 0.143862747145711 1106 62-63 0.05384826137968446 1106 64-65 0.3624565732535956 1106 66-67 0.13636124158849583 1106 68-69 0.4116573288189187 1106 70-71 0.37769202982344297 1106 72-73 0.07954838080902249 1106 74-75 -0.44713368421707145 1106 76 0.4435127518930102 1107 1 0.07591221772966605 1107 2 -0.05007634219968082 1107 3 0.0231195169105618 1107 4 0.05068098837721635 1107 5 0.053000594362828224 1107 6 0.07504414542510318 1107 7 0.23682163730776296 1107 8 0.14355594060159405 1107 9 0.22215230747836756 1107 10-11 0.05498545081590578 1107 12-13 0.09711170212155196 1107 14-15 0.46698726924638123 1107 16-17 0.3969138657891307 1107 18-19 -0.04612470238330246 1107 20-21 0.19186331034016035 1107 22-23 0.10476051064615888 1107 24-25 0.2397146798051324 1107 26-27 0.08063024017413056 1107 28-29 0.28924437575092554 1107 30-31 0.24674398744718928 1107 32-33 0.17707917119414418 1107 34-35 0.3055964974869738 1107 36-37 0.3609431831623837 1107 38-39 0.038948086019310324 1107 40-41 0.3217493332258883 1107 42-43 0.32316137507291387 1107 44-45 0.23917784887078852 1107 46-47 0.22361773818179387 1107 48-49 0.22613782371060864 1107 50-51 0.23413989835089666 1107 52-53 0.3732337635353602 1107 54-55 0.3715776145548446 1107 56-57 0.020938978283986387 1107 58-59 0.4698197297341622 1107 60-61 0.10925091246208041 1107 62-63 0.1413328783420411 1107 64-65 0.5154216537581604 1107 66-67 0.1633392438446819 1107 68-69 0.3404402741578565 1107 70-71 0.07512049869843196 1107 72-73 -0.18693710785880313 1107 74-75 -0.3346003543021894 1107 76 0.02861940762063142 1108 1 -0.34900722373112814 1108 2 -0.12048450546498657 1108 3 -0.030263941736059508 1108 4 -0.02595703525329185 1108 5 0.00880080918560111 1108 6 0.0729227705593658 1108 7 0.19130606265684946 1108 8 0.30196088152533207 1108 9 0.032168419186206165 1108 10-11 0.11210145511235936 1108 12-13 0.14430557967257585 1108 14-15 0.10646095345691009 1108 16-17 0.3013982911382129 1108 18-19 0.2029085951462335 1108 20-21 0.29876449186540555 1108 22-23 -0.03276634220024022 1108 24-25 0.29003691396195563 1108 26-27 0.15200510590990035 1108 28-29 0.10600055190560198 1108 30-31 0.1607343204224776 1108 32-33 0.03616885970112804 1108 34-35 0.060470289108884856 1108 36-37 0.1512493056113584 1108 38-39 -0.48759595264878897 1108 40-41 -0.04989406097389093 1108 42-43 0.1381855426346803 1108 44-45 0.056511361223094525 1108 46-47 0.04715425805289897 1108 48-49 0.06605457988676022 1108 50-51 0.20222529040245973 1108 52-53 0.09317200198864128 1108 54-55 0.22334990241735397 1108 56-57 0.06872361845584152 1108 58-59 0.07306892414877098 1108 60-61 0.3012219113879766 1108 62-63 0.18248486545267895 1108 64-65 0.43058008555192373 1108 66-67 0.047482638044463954 1108 68-69 0.10046981228889962 1108 70-71 0.12026013349972686 1108 72-73 -0.3622593420156264 1108 74-75 -0.7251884316383865 1108 76 -0.16415717669730867 1109 1 0.054421803382112444 1109 2 -0.0994101252996984 1109 3 0.09927063851622364 1109 4 -0.02244826332346861 1109 5 0.22883125720647968 1109 6 -0.040285870878939534 1109 7 0.20982860869136033 1109 8 0.05026449179066361 1109 9 0.22109113920940615 1109 10-11 0.05037534230969243 1109 12-13 0.2441678386257422 1109 14-15 0.25762087806180745 1109 16-17 0.1657197352443518 1109 18-19 0.3591431898975799 1109 20-21 0.30493746562211044 1109 22-23 0.13780782047918905 1109 24-25 0.3376373199355669 1109 26-27 0.3142278114340371 1109 28-29 0.3498855743791367 1109 30-31 0.32851016499257923 1109 32-33 0.2869663078669973 1109 34-35 0.14024047904653258 1109 36-37 0.21715839652045332 1109 38-39 0.03205751987550087 1109 40-41 -0.07999772923545123 1109 42-43 0.07639055930859229 1109 44-45 0.05908494394022057 1109 46-47 0.2527436447427647 1109 48-49 0.2313909580221818 1109 50-51 0.1758986266356004 1109 52-53 0.06382590948464895 1109 54-55 0.23935323720059642 1109 56-57 0.3107890092054433 1109 58-59 0.3080442757509161 1109 60-61 0.25227671782555205 1109 62-63 0.23488083471467291 1109 64-65 0.21240189213450122 1109 66-67 0.4757268021737886 1109 68-69 0.4460475997327151 1109 70-71 0.6525857823325509 1109 72-73 0.09048460462783936 1109 74-75 0.3067493674046773 1109 76 0.2551120708016015 1110 1 0.23121834017864273 1110 2 0.19237263739215393 1110 3 -0.028309054518011578 1110 4 0.06231364143843621 1110 5 -0.07353001788207081 1110 6 0.056982920935311654 1110 7 0.13243388220572427 1110 8 0.25406614468322886 1110 9 0.161642103396737 1110 10-11 0.11274055285672091 1110 12-13 0.11859129395828916 1110 14-15 -0.020155587896475424 1110 16-17 -0.0910453178843369 1110 18-19 0.08734468537180362 1110 20-21 0.035944942993218376 1110 22-23 -0.13141295874159198 1110 24-25 0.3395105981724811 1110 26-27 0.07899758711290872 1110 28-29 0.3179178451386804 1110 30-31 -0.06580703296097568 1110 32-33 0.12233427323496215 1110 34-35 -0.04588309434976168 1110 36-37 0.10482073418278759 1110 38-39 -0.08549068949089644 1110 40-41 0.2273615781238547 1110 42-43 -0.04617535962096753 1110 44-45 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-0.0018721316362260154 2116 5 -0.013388924407642833 2116 6 -0.09337569164552662 2116 7 0.14728397038913954 2116 8 -0.07836183885586934 2116 9 -0.41292579783783623 2116 10-11 -0.26860571404510125 2116 12-13 -0.29293721868133105 2116 14-15 -0.21202507643263147 2116 16-17 -0.5223563114175533 2116 18-19 -0.36530669616847433 2116 20-21 -0.331715256889197 2116 22-23 -0.13847351135770225 2116 24-25 -0.10073631540776518 2116 26-27 -0.23410059042971199 2116 28-29 -0.10916622305649781 2116 30-31 -0.38969886129724784 2116 32-33 -0.11333298132118585 2116 34-35 -0.16767027835916792 2116 36-37 -0.0959729166108616 2116 38-39 -0.45022319977896075 2116 40-41 -0.6456542948920188 2116 42-43 -0.4607315030659933 2116 44-45 -0.5962393426971957 2116 46-47 -0.3648123869546822 2116 48-49 -0.2241067803038348 2116 50-51 -0.3848604795780446 2116 52-53 -0.2566005776085021 2116 54-55 -0.6644831060296852 2116 56-57 -0.6259861497919346 2116 58-59 -0.1716516736420033 2116 60-61 -0.6112916255407725 2116 62-63 -0.27703971760992374 2116 64-65 -0.3586282748552634 2116 66-67 -0.14883531733782007 2116 68-69 -0.4992811082222559 2116 70-71 -0.15471279264279048 2116 72-73 -0.08414043363355717 2116 74-75 0.19657353500662822 2116 76 0.23300548303844693 2117 1 -0.34809897966965053 2117 2 -0.36251990369508036 2117 3 -0.1020385109023394 2117 4 -0.01212377954765742 2117 5 -0.11739853874173889 2117 6 -0.2434721991658293 2117 7 -0.3351261683631819 2117 8 -0.9537214659362405 2117 9 -0.4482694010280426 2117 10-11 -0.4074490805187523 2117 12-13 -0.28268557076989964 2117 14-15 -0.19009237677131807 2117 16-17 -0.3885295151030519 2117 18-19 -0.41153015660039216 2117 20-21 -0.48648236421283997 2117 22-23 -0.022892099070297434 2117 24-25 -0.4120823221815044 2117 26-27 -0.6043526025204642 2117 28-29 -1.3553059222443764 2117 30-31 -0.544365819307373 2117 32-33 -0.8213066875741433 2117 34-35 -0.5190942195077852 2117 36-37 -0.2808303404063395 2117 38-39 -0.4224818399333685 2117 40-41 -0.46448418673076475 2117 42-43 -0.7554800372442259 2117 44-45 -0.5305869154420151 2117 46-47 -0.43505347366577496 2117 48-49 -0.5356803991537546 2117 50-51 -0.4137854222198101 2117 52-53 -0.6486305131487669 2117 54-55 -0.5772439494690005 2117 56-57 -0.6013858365977214 2117 58-59 -0.23299768041573543 2117 60-61 -0.453237799836991 2117 62-63 -0.3801024657070826 2117 64-65 -0.8171027276758309 2117 66-67 -0.2655821034553085 2117 68-69 -0.6729582268354619 2117 70-71 -0.4973691166167171 2117 72-73 -0.36478612357128526 2117 74-75 0.17119023627032703 2117 76 -0.3734259237862716 2118 1 0.050351247072747185 2118 2 -0.1923750527286714 2118 3 -0.42570777520031555 2118 4 -0.10205025337321416 2118 5 -0.3321654123383624 2118 6 -0.10529142163753846 2118 7 -0.10294635944318031 2118 8 -0.15618261153567659 2118 9 -0.4022384936181922 2118 10-11 -0.36998155091016116 2118 12-13 -0.26738596261598246 2118 14-15 -0.38127143722127954 2118 16-17 -0.6686003996185264 2118 18-19 -0.5279502684306152 2118 20-21 -0.37431092054622184 2118 22-23 -0.6279090497152922 2118 24-25 -0.3687481082951791 2118 26-27 -0.38231015986647776 2118 28-29 -0.17482558058981823 2118 30-31 -0.3156862085117922 2118 32-33 -0.1449649449597814 2118 34-35 0.04429458866373892 2118 36-37 -0.1299695998612762 2118 38-39 -0.20942103774959975 2118 40-41 -0.2484735320396183 2118 42-43 -0.4940218414972932 2118 44-45 -0.7319803905443791 2118 46-47 -0.3581481771395545 2118 48-49 -0.3003305994795582 2118 50-51 0.060689548061525045 2118 52-53 -0.28737264320643874 2118 54-55 -0.007306028454600266 2118 56-57 -0.14398258735719338 2118 58-59 0.12252558819695025 2118 60-61 -0.2714476356138604 2118 62-63 0.10435577406749985 2118 64-65 -0.24942122369132846 2118 66-67 0.04544021856292346 2118 68-69 0.08191783533158059 2118 70-71 -0.07569430483491857 2118 72-73 -0.2239967078181948 2118 74-75 0.7330270698017856 2118 76 0.31107194087273626 2119 1 -0.27038880267849663 2119 2 -0.1590261721316537 2119 3 0.0729707073867516 2119 4 -0.04848992999013291 2119 5 -0.562964541691592 2119 6 -0.23798731715992716 2119 7 -0.14997683207999302 2119 8 -0.174683855316772 2119 9 0.061225436730069305 2119 10-11 -0.3005630185718502 2119 12-13 -0.22288192032742415 2119 14-15 -1.0824918974202902 2119 16-17 -0.5732179369319539 2119 18-19 -0.39572079081867884 2119 20-21 -0.1959449379591618 2119 22-23 -0.1886599825511226 2119 24-25 -0.9371560684941898 2119 26-27 -0.3826988414585202 2119 28-29 -0.4523053691470338 2119 30-31 -0.7874290567704989 2119 32-33 -1.0196151315269475 2119 34-35 -0.49457357054023277 2119 36-37 -0.46489652772196877 2119 38-39 0.30138431050910697 2119 40-41 -0.2611056837809116 2119 42-43 -0.21510393104953351 2119 44-45 -0.4868000422856724 2119 46-47 -0.3446220577365722 2119 48-49 -0.38424695520095753 2119 50-51 -0.4375225166150898 2119 52-53 -0.2972062874850465 2119 54-55 -0.46319066775807727 2119 56-57 -0.6042204604915256 2119 58-59 -0.7242172600617565 2119 60-61 -0.555768220190977 2119 62-63 -0.8336883801613624 2119 64-65 -1.2458453530445581 2119 66-67 -0.7407849058151896 2119 68-69 -0.5266409333251403 2119 70-71 -0.041801270009045766 2119 72-73 -0.0074621928928237935 2119 74-75 -0.05972790532259609 2119 76 -1.5855387556446772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 6.0 23 9.0 24 24.0 25 51.0 26 110.0 27 182.0 28 341.0 29 668.0 30 1070.0 31 1782.0 32 2899.0 33 4625.0 34 8029.0 35 12099.0 36 20254.0 37 72568.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.976932513369846 19.957344793579267 12.923244682130516 30.142478010920374 2 19.55564820679757 21.367692690084265 34.69744469656273 24.379214406555434 3 18.56383447855614 22.004313628017734 30.92342107584128 28.508430817584852 4 14.036930428716895 22.455721169650662 35.73656401087227 27.770784390760177 5 13.87176177227572 33.37769902422206 38.01204287970751 14.738496323794708 6 27.579156677704635 37.95271044972378 18.248731167966902 16.219401704604678 7 26.476695985439502 27.616840788640246 24.215649329303005 21.69081389661725 8 23.339293302651516 35.80471612639411 18.977557909253452 21.878432661700916 9 23.031406098411654 17.319457028086692 20.763143335925786 38.88599353757587 10-11 25.45000440984277 27.02832722636926 25.638424964520812 21.883243399267162 12-13 25.0378845583342 21.813086809759383 26.05214839521813 27.096880236688285 14-15 22.097321220965192 22.16547333648704 22.615277298931215 33.12192814361655 16-17 24.411686885127605 27.04235854427081 22.931984188709198 25.613970381892383 18-19 25.656465230394236 25.985198964087846 24.343133874808572 24.015201930709342 20-21 23.06989199894164 25.6039480119627 23.710521884846976 27.615638104248685 22-23 22.716703682619606 27.35866453925161 23.690076250190426 26.234555527938358 24-25 24.475830052677576 27.729091331852697 24.441753994916652 23.353324620553074 26-27 25.13369841486197 28.510034396773598 24.157519583710844 22.198747604653587 28-29 22.366321629877888 27.07803818122048 26.755718764281877 23.799921424619754 30-31 22.06484874239302 28.556538193247327 26.760128607050937 22.618484457308714 32-33 23.22664186464188 28.162057712815002 25.745463875369822 22.86583654717329 34-35 23.267533133954988 24.820198683461488 29.78287537784335 22.12939280474018 36-37 23.32365840556121 27.835328453107334 26.697989913486904 22.143023227844548 38-39 21.515221975449204 27.17144666896513 27.677375903015534 23.635955452570137 40-41 22.159860809326418 23.540141596042368 28.289141363523385 26.01085623110783 42-43 24.17596074438146 23.41065257655086 27.910295780181364 24.503090898886313 44-45 21.09307975401095 26.06898597669999 29.53271702439846 23.305217244890596 46-47 22.887885761018595 23.84482164190473 29.00072962853088 24.266562968545795 48-49 22.599241507043722 23.31804587840059 27.161825193832634 26.920887420723055 50-51 22.017944051122104 23.74299436341915 28.16446308159813 26.074598503860617 52-53 19.628611059885664 23.811146478940998 32.725844083995476 23.834398377177862 54-55 19.98580832417957 24.933251016268308 29.60608077228374 25.47485988726838 56-57 21.252635883291507 25.52537263171398 27.331403693042873 25.890587791951635 58-59 19.94531794966365 25.498111785505245 29.657395306323714 24.899174958507388 60-61 21.40938574899175 27.58597188925682 27.233585362529166 23.771056999222264 62-63 19.261391425662076 31.167566007328357 25.22991316618693 24.341129400822638 64-65 19.356002597798287 32.29648575620786 24.036850249757457 24.310661396236398 66-67 19.13230330096776 31.723206196229984 24.151907056550222 24.992583446252034 68-69 18.90178879258505 32.25198643372006 23.97671603017936 24.869508743515524 70-71 19.19404109973461 31.428949415094493 24.23850033274268 25.138509152428217 72-73 19.334354278750173 30.26875987203438 24.366786667842625 26.030099181372822 74-75 19.14152388130307 30.168536172737547 24.80135662799368 25.8885833179657 76 19.351191860232035 30.609921344440792 24.94126891221206 25.097617883115113 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 2.5 16 4.5 17 5.0 18 6.5 19 11.0 20 20.0 21 26.0 22 36.5 23 60.0 24 87.5 25 102.0 26 191.5 27 326.0 28 466.0 29 561.0 30 662.5 31 948.0 32 1375.5 33 1619.0 34 1876.0 35 2560.0 36 4103.0 37 5219.0 38 6256.5 39 7570.5 40 7897.0 41 7661.5 42 7376.0 43 7376.5 44 7666.5 45 7863.5 46 7771.0 47 7762.0 48 7462.5 49 6742.0 50 6312.0 51 5802.0 52 4620.0 53 3639.0 54 3330.0 55 3276.5 56 2747.0 57 2152.5 58 2034.0 59 1824.5 60 1537.0 61 1438.0 62 1417.0 63 1365.0 64 1361.0 65 1409.0 66 1372.5 67 1336.0 68 1290.0 69 1211.5 70 989.0 71 799.0 72 708.5 73 495.0 74 285.0 75 198.0 76 138.5 77 48.0 78 10.5 79 4.0 80 3.0 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 124721.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.669510347094715 #Duplication Level Percentage of deduplicated Percentage of total 1 61.18517904165544 23.660009140401375 2 16.697422712475895 12.91362320699802 3 6.759418607062141 7.84150223298402 4 3.705239586141119 5.731192020589957 5 2.5026436376454 4.838800202050978 6 1.884758133073462 4.37296044771931 7 1.4825105227145494 4.012956919845094 8 1.0657488233220678 3.2969588120685374 9 0.8438906052375126 2.9369552841943216 >10 3.8275726222812 22.600043296638095 >50 0.03110161935764789 0.7857538024871513 >100 0.006220323871529578 0.5187578675603948 >500 0.0 0.0 >1k 0.008293765162039437 6.4904867664627455 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 3954 3.170276056157343 No Hit CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG 2091 1.676542041837381 Illumina PCR Primer Index 8 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 1042 0.8354647573383793 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 1008 0.8082039111296414 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 325 0.26058161817175934 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 208 0.166772235629926 RNA PCR Primer, Index 15 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005612527160622509 0.0 2 0.0 0.0 0.0 0.00962147513249573 0.0 3 0.0 0.0 0.0 0.00962147513249573 0.0 4 0.0 0.0 0.0 0.03046800458623648 0.0 5 0.0 0.0 0.0 0.03126979418061113 0.0 6 0.0 8.017895943746442E-4 0.0 0.03126979418061113 0.0 7 0.0 8.017895943746442E-4 0.0 0.03126979418061113 0.0 8 0.0 8.017895943746442E-4 0.0 0.03126979418061113 0.0 9 0.0 8.017895943746442E-4 0.0 0.035278742152484346 0.0 10 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 11 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 12 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 13 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 14 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 15 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 16 0.0 8.017895943746442E-4 0.0 0.03768411093560828 0.0 17 0.0 8.017895943746442E-4 0.0 0.03848590052998292 0.0 18 0.0 8.017895943746442E-4 0.0 0.04008947971873221 0.0 19 0.0 8.017895943746442E-4 0.0 0.044900217284980073 0.0 20 0.0 8.017895943746442E-4 0.0 0.047305586068104005 0.0 21 0.0 8.017895943746442E-4 0.0 0.0489091652568533 0.0 22 0.0 8.017895943746442E-4 0.0 0.05051274444560259 0.0 23 0.0 8.017895943746442E-4 0.0 0.05131453403997723 0.0 24 0.0 8.017895943746442E-4 0.0 0.053719902823101164 0.0 25 0.0 8.017895943746442E-4 0.0 0.05692706120059974 0.0 26 0.0 8.017895943746442E-4 0.0 0.06414316754997154 0.0 27 0.0 8.017895943746442E-4 0.0 0.1234755975336952 0.0 28 0.0 8.017895943746442E-4 0.0 0.17318655238492314 0.0 29 0.0 8.017895943746442E-4 0.0 0.22369929683052572 0.0 30 0.0 8.017895943746442E-4 0.0 0.2886442539748719 0.0 31 0.0 8.017895943746442E-4 0.0 0.35278742152484344 0.0 32 0.0 8.017895943746442E-4 0.0 0.42334490582981216 0.0 33 0.0 8.017895943746442E-4 0.0 0.5107399716166484 0.0 34 0.0 8.017895943746442E-4 0.0 0.6518549402265857 0.0 35 0.0 8.017895943746442E-4 0.0 0.8498969700371228 0.0 36 0.0 8.017895943746442E-4 0.0 1.144955540766992 0.0 37 0.0 8.017895943746442E-4 0.0 1.5674986570024294 0.0 38 0.0 8.017895943746442E-4 0.0 2.0181044090409794 0.0 39 0.0 8.017895943746442E-4 0.0 2.5200246951195067 0.0 40 0.0 8.017895943746442E-4 0.0 3.0820792007761324 0.0 41 0.0 8.017895943746442E-4 0.0 3.7259162450589716 0.0 42 0.0 8.017895943746442E-4 0.0 4.4467250904017765 0.0 43 0.0 8.017895943746442E-4 0.0 5.200407309113943 0.0 44 0.0 8.017895943746442E-4 0.0 5.910792889729877 0.0 45 0.0 8.017895943746442E-4 0.0 6.682114479518285 0.0 46 0.0 8.017895943746442E-4 0.0 7.436598487824825 0.0 47 0.0 8.017895943746442E-4 0.0 8.100480271967031 0.0 48 0.0 8.017895943746442E-4 0.0 8.729083313956751 0.0 49 0.0 8.017895943746442E-4 0.0 9.384145412560835 0.0 50 0.0 8.017895943746442E-4 0.0 10.01675740252243 0.0 51 0.0 8.017895943746442E-4 0.0 10.680639186664635 0.0 52 0.0 8.017895943746442E-4 0.0 11.413474875923061 0.0 53 0.0 8.017895943746442E-4 0.0 12.13668909004899 0.0 54 0.0 8.017895943746442E-4 0.0 12.867921200118666 0.0 55 0.0 8.017895943746442E-4 0.0 13.521379719534 0.0 56 0.0 8.017895943746442E-4 0.0 14.201297295563698 0.0 57 0.0 8.017895943746442E-4 0.0 14.720856952718467 0.0 58 0.0 8.017895943746442E-4 0.0 15.287722195941342 0.0 59 0.0 8.017895943746442E-4 0.0 15.861803545513586 0.0 60 0.0 8.017895943746442E-4 0.0 16.463145741294568 0.0 61 0.0 8.017895943746442E-4 0.0 17.050857513971184 0.0 62 0.0 8.017895943746442E-4 0.0 17.641776445025297 0.0 63 0.0 8.017895943746442E-4 0.0 18.27599201417564 0.0 64 0.0 8.017895943746442E-4 0.0 18.89577537062724 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATTAC 20 7.9009755E-5 70.0 43 TTTCGCC 20 0.0069375853 52.5 33 TTATGCA 20 0.0069375853 52.5 50 ACGATGC 20 0.0069375853 52.5 8 ATGGTCA 20 0.0069375853 52.5 14 CCGAAGA 20 0.0069375853 52.5 29 GGCGCAT 20 0.0069375853 52.5 10 TAAGCCA 20 0.0069375853 52.5 43 TGGTCAT 20 0.0069375853 52.5 15 TACTTAT 30 5.8591506E-4 46.666668 47 TATGACA 30 5.8591506E-4 46.666668 53 ACTAAGC 40 4.5711666E-5 43.75 41 CTAGAAC 35 0.0012514691 40.0 64 ACACAGT 35 0.0012514691 40.0 34 TTACTTA 35 0.0012514691 40.0 46 TACATAC 35 0.0012514691 40.0 43 GGATATT 45 9.154789E-5 38.88889 1 TCCATAC 40 0.0024111997 35.0 64 TTTACAC 40 0.0024111997 35.0 3 CTTATAT 40 0.0024111997 35.0 49 >>END_MODULE