FastQCFastQC Report
Wed 25 May 2016
SRR1780223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792405
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAT407.070724E-427.4728243
TATCGGA609.7840875E-625.65425326
GAAAATT901.5186743E-824.43262326
GGCTAAA450.001407932524.4141221
AACTTTT551.5947566E-423.9959722
CGCAAAC551.6022882E-423.97930731
TAACACG703.225568E-521.9893625
ATCGGAA703.225568E-521.9893627
CCGCAAA602.8809754E-421.98935930
TTGCGTG904.114645E-721.97964532
GTGATTC755.4394717E-520.54805640
TTATCGG755.4899865E-520.52340325
TAAGCGC755.5220997E-520.5078624
CACGTAA654.8667094E-420.34669518
TATACCG1201.4480975E-820.1441946
ATTGTAC550.004432766720.0384115
TTGGGCC550.004507992419.98023442
GTATAAG2050.019.293111
GTAAGCG809.069619E-519.2261223
TAGATCT2200.019.01362239
Produced by FastQC (version 0.11.5)