FastQCFastQC Report
Wed 25 May 2016
SRR1780223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792405
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT211502.6690896700550857No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC68810.8683690789432171No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG52760.665821139442583No Hit
CACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTG45030.568270013440097Illumina PCR Primer Index 1 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC11480.1448754109325408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT6500.035.53637341
AATATCG505.9480953E-835.1957136
ATATCGT505.9480953E-835.1957137
CGTCTTC6650.034.73480242
GTCTTCT6800.034.292143
ATCGTAT551.3741919E-731.99609839
TCTTCTG7300.031.64197344
ATGCCGT31600.031.39688944
GCCGTCT7600.030.3929540
CAGTTGT300.00566793629.4132751
CATCGAT300.00574194229.3353121
GAATAAG300.005747257729.3297636
TACGTAG300.005747257729.3297614
CTTTATA1200.027.5035922
TATGCCG36550.027.20496643
CGCAAAG508.344786E-526.39678212
CGGTATA450.001398782224.44146336
TGCCGTC10000.023.31715839
TCTTTAT1550.022.771571
AATCTCG43900.022.09753436
Produced by FastQC (version 0.11.5)