FastQCFastQC Report
Wed 25 May 2016
SRR1780222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169962
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTC150.002221634870.09
AGGCATC150.002221634870.08
CGGTACG150.002221634870.014
CGCAACT150.002221634870.068
GCCGGTA200.006941899752.512
CAACTTG200.006941899752.570
GTTATAG200.006941899752.52
GGATAAA305.8651954E-446.66666432
TACAATT305.8651954E-446.6666647
GGACTAG404.5782173E-543.751
GCGAAAT350.001252752440.011
GGCACGA350.001252752440.027
TTAGATA350.001252752440.02
GGCAACT350.001252752440.030
CTCCAAT350.001252752440.012
GGCGAAA350.001252752440.010
CATCGTT459.1688424E-538.88888532
GACTAGG459.1688424E-538.8888852
AGTCATC400.002413656835.08
GAGTCAT400.002413656835.041
Produced by FastQC (version 0.11.5)