##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780222_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 169962 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.694225768112872 34.0 32.0 34.0 21.0 34.0 2 30.82886762923477 34.0 32.0 34.0 21.0 34.0 3 30.60315835304362 34.0 32.0 34.0 21.0 34.0 4 30.51312057989433 34.0 32.0 34.0 21.0 34.0 5 30.556159612148598 34.0 32.0 34.0 11.0 34.0 6 33.602205198809145 38.0 34.0 38.0 21.0 38.0 7 33.9658688412704 38.0 34.0 38.0 21.0 38.0 8 34.02784151751568 38.0 35.0 38.0 21.0 38.0 9 34.16846706910957 38.0 36.0 38.0 21.0 38.0 10-11 33.93848919170168 38.0 34.5 38.0 21.0 38.0 12-13 33.9869735587955 38.0 35.0 38.0 21.0 38.0 14-15 33.899574610795355 38.0 34.5 38.0 21.0 38.0 16-17 33.96542756616185 38.0 35.0 38.0 21.0 38.0 18-19 33.98806497923066 38.0 35.0 38.0 21.0 38.0 20-21 33.97858933173298 38.0 35.0 38.0 21.0 38.0 22-23 33.9937162424542 38.0 35.0 38.0 21.0 38.0 24-25 34.034184111742626 38.0 35.5 38.0 21.0 38.0 26-27 34.02155187630176 38.0 35.0 38.0 21.0 38.0 28-29 34.08743719184288 38.0 36.0 38.0 21.0 38.0 30-31 34.106873889457646 38.0 36.0 38.0 21.0 38.0 32-33 34.15253409585672 38.0 36.0 38.0 21.0 38.0 34-35 34.124301314411454 38.0 36.0 38.0 21.0 38.0 36-37 34.069362563396524 38.0 35.5 38.0 21.0 38.0 38-39 34.04459820430449 38.0 35.5 38.0 21.0 38.0 40-41 34.11993269083678 38.0 36.0 38.0 21.0 38.0 42-43 34.16910250526588 38.0 36.0 38.0 21.0 38.0 44-45 34.22685659147339 38.0 36.0 38.0 21.0 38.0 46-47 34.17488909285605 38.0 36.0 38.0 21.0 38.0 48-49 34.13428001553288 38.0 36.0 38.0 21.0 38.0 50-51 34.08043856862122 38.0 36.0 38.0 21.0 38.0 52-53 34.11436379896683 38.0 36.0 38.0 21.0 38.0 54-55 34.108177121944905 38.0 36.0 38.0 21.0 38.0 56-57 34.05802179310669 38.0 36.0 38.0 21.0 38.0 58-59 34.11715265765289 38.0 36.0 38.0 21.0 38.0 60-61 34.136474623739424 38.0 36.0 38.0 21.0 38.0 62-63 34.02497322931008 38.0 36.0 38.0 21.0 38.0 64-65 34.01272343229663 38.0 35.5 38.0 20.0 38.0 66-67 33.92484202351114 38.0 35.0 38.0 20.0 38.0 68-69 33.88048504959932 38.0 35.0 38.0 20.0 38.0 70-71 33.913510078723476 38.0 35.0 38.0 20.0 38.0 72-73 33.9487002977136 38.0 35.0 38.0 20.0 38.0 74-75 33.968237017686306 38.0 36.0 38.0 20.0 38.0 76 32.58782551393841 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05066574241128308 1101 2 0.5197731068006277 1101 3 0.5392794849339104 1101 4 0.7045916434792332 1101 5 0.16598486759374964 1101 6 0.5320512428770314 1101 7 0.4028083570364842 1101 8 0.06712304612257469 1101 9 -0.07685262259760606 1101 10-11 0.39266405158208784 1101 12-13 0.40839583761041354 1101 14-15 0.6442159924760631 1101 16-17 0.35464049349806714 1101 18-19 0.09257046161412319 1101 20-21 0.07441615305796034 1101 22-23 0.15395180463480074 1101 24-25 0.35028301143877627 1101 26-27 -0.09330837790675872 1101 28-29 -0.44609858099283883 1101 30-31 0.2168571214414854 1101 32-33 0.31878447132824306 1101 34-35 0.2373642017260309 1101 36-37 0.2277205137503273 1101 38-39 0.21129737435959584 1101 40-41 0.5566255785072869 1101 42-43 0.3525380237811504 1101 44-45 0.144766959031827 1101 46-47 0.3181843677048093 1101 48-49 0.13779902407079447 1101 50-51 0.09333317906256156 1101 52-53 0.3480039847831691 1101 54-55 0.31833591157391794 1101 56-57 0.48547584731354476 1101 58-59 0.4502688338243601 1101 60-61 0.31301186775825585 1101 62-63 0.1024839292153672 1101 64-65 0.23962877510991376 1101 66-67 0.26763480175940657 1101 68-69 0.08944122681040056 1101 70-71 0.2160667943958785 1101 72-73 0.1955155211470796 1101 74-75 0.11898998612543465 1101 76 -0.9484065285072347 1102 1 0.4248027744271319 1102 2 0.21295279185642002 1102 3 0.2177131067922886 1102 4 0.18321298042782175 1102 5 -0.44758276198180624 1102 6 -0.06582131943395098 1102 7 -0.07534369218658554 1102 8 0.06933391274057499 1102 9 0.17408073854537065 1102 10-11 -0.007377662882390723 1102 12-13 0.07609841347859003 1102 14-15 -0.14196609116143577 1102 16-17 0.03610884264813308 1102 18-19 0.10952739142954471 1102 20-21 0.27187678503209867 1102 22-23 -0.24301868238157454 1102 24-25 0.007202398236145768 1102 26-27 0.07003503642216202 1102 28-29 -0.14318899709462585 1102 30-31 0.1259039459278739 1102 32-33 0.048021096628069415 1102 34-35 0.10248048079579775 1102 36-37 0.2774910841956242 1102 38-39 0.012606165682989001 1102 40-41 -0.055893975147945696 1102 42-43 0.008101690482583024 1102 44-45 0.1877954291538444 1102 46-47 -0.2842924286243189 1102 48-49 -0.07542446117270885 1102 50-51 -0.029072702676934625 1102 52-53 -0.1772071946933167 1102 54-55 -0.20881499050137364 1102 56-57 -0.24369299198147587 1102 58-59 -0.08853604676798454 1102 60-61 0.004460402537695529 1102 62-63 -0.08198786137802472 1102 64-65 0.018771542864634227 1102 66-67 0.1645792172354703 1102 68-69 -0.0802219288888324 1102 70-71 -0.2501236321345175 1102 72-73 -0.03952462902499576 1102 74-75 -0.07822035906175984 1102 76 -0.03613204733088793 1103 1 0.11261839273602448 1103 2 0.11411628719032052 1103 3 -0.13440582828628678 1103 4 0.3868145640742462 1103 5 0.005917823805773992 1103 6 -0.6190067919019313 1103 7 -0.2849142492907788 1103 8 -0.10548328113491579 1103 9 -0.053932027583982745 1103 10-11 -0.0670572976741397 1103 12-13 0.021731266062118948 1103 14-15 -0.3038303096400057 1103 16-17 0.07692207740755919 1103 18-19 -0.1611064711325767 1103 20-21 0.015286674742434059 1103 22-23 0.10726209166602274 1103 24-25 -0.46083682933223713 1103 26-27 -0.4472052980827499 1103 28-29 -0.26262906496518923 1103 30-31 -0.15883426762199804 1103 32-33 -0.021818922763522153 1103 34-35 -0.6159879876726748 1103 36-37 -0.052999159411207586 1103 38-39 -0.6625241215549238 1103 40-41 -0.25806643909170646 1103 42-43 -0.3387148570066074 1103 44-45 -0.13892254442512098 1103 46-47 -0.32185858222186425 1103 48-49 -0.4116614829478422 1103 50-51 -0.6455888825339215 1103 52-53 -0.10166079627696689 1103 54-55 -0.26197521350464825 1103 56-57 -0.4673578002875374 1103 58-59 -0.4923406361106899 1103 60-61 -0.10261247751724767 1103 62-63 -0.24068074708884524 1103 64-65 -0.09807288003359815 1103 66-67 -0.15571529252092375 1103 68-69 -0.2823741932608357 1103 70-71 -0.23225711090476864 1103 72-73 -0.3021746108453769 1103 74-75 -0.2563452328144322 1103 76 0.12426265230148203 1104 1 -0.09835437778730594 1104 2 0.0715107603151317 1104 3 0.23081332344971628 1104 4 0.3735490513705315 1104 5 -0.5235892113192371 1104 6 -0.2755003641258398 1104 7 -0.17652271168575595 1104 8 -0.3414737660018048 1104 9 -0.2610177650067982 1104 10-11 -0.10175716608187457 1104 12-13 0.07762761184611122 1104 14-15 -0.22215453273950914 1104 16-17 -0.27948545371907585 1104 18-19 -0.14627197371380873 1104 20-21 -0.7881524385281367 1104 22-23 -0.44242917263198933 1104 24-25 -0.4285385197867342 1104 26-27 -0.3194646360724249 1104 28-29 -0.657831008481736 1104 30-31 -0.19367332015767857 1104 32-33 -0.21097774450661433 1104 34-35 -0.12167682257499024 1104 36-37 -0.13176472272733264 1104 38-39 -0.3444251236806437 1104 40-41 -0.30731484480078564 1104 42-43 0.12103815443178689 1104 44-45 -0.011151514968695153 1104 46-47 -0.10584228502579407 1104 48-49 -0.443726975406598 1104 50-51 -0.4714695384707497 1104 52-53 -0.18727168806763217 1104 54-55 -0.5853353714153755 1104 56-57 -0.6867347615184372 1104 58-59 -0.3029059767322124 1104 60-61 -0.14446134886050999 1104 62-63 0.025922654065119843 1104 64-65 -0.04850814676100157 1104 66-67 0.09411768652553576 1104 68-69 -0.09961024954183273 1104 70-71 -0.3440194350266452 1104 72-73 0.07158032386460889 1104 74-75 -0.23225119495635482 1104 76 0.16298620731716795 1105 1 0.5434610000492199 1105 2 -0.22007084111445607 1105 3 0.32493586634952365 1105 4 0.46187491854636775 1105 5 0.7020845787960575 1105 6 0.18470868828413955 1105 7 0.4787317496493415 1105 8 0.45827165026707917 1105 9 0.16982113734565019 1105 10-11 0.41960583604781476 1105 12-13 -0.5390353829398222 1105 14-15 0.14101895138572473 1105 16-17 0.03221560623049413 1105 18-19 0.29754335516431496 1105 20-21 0.31494662881415536 1105 22-23 0.3454120062865229 1105 24-25 0.3182290012041875 1105 26-27 0.6000036755968097 1105 28-29 0.23742981744663894 1105 30-31 0.4412731927795832 1105 32-33 0.4800653144148299 1105 34-35 0.3622844023644305 1105 36-37 0.2906606768956834 1105 38-39 0.3490462723684118 1105 40-41 0.21963920773158208 1105 42-43 0.49429462306169114 1105 44-45 0.27691976847602007 1105 46-47 0.44918451970359285 1105 48-49 0.4433773794439517 1105 50-51 0.3549484194739776 1105 52-53 0.12720041787836323 1105 54-55 0.3046281545693077 1105 56-57 0.5133802907446707 1105 58-59 0.4847320247855009 1105 60-61 0.38684021046484673 1105 62-63 0.4642815681675927 1105 64-65 -0.3064724560802361 1105 66-67 0.37072291038786886 1105 68-69 0.6347267311930338 1105 70-71 0.6173866505036969 1105 72-73 0.3967669778020877 1105 74-75 0.26942318265986387 1105 76 0.27127731402202215 1106 1 -0.10216104187099617 1106 2 -0.15784317226913913 1106 3 0.38596152182244126 1106 4 0.6151269281624749 1106 5 0.2330458138647984 1106 6 0.19609494776877057 1106 7 -0.41705932540136814 1106 8 0.07918198758208206 1106 9 0.4092941296076873 1106 10-11 0.3492598815740706 1106 12-13 0.4882775537290982 1106 14-15 0.30482144556347635 1106 16-17 0.395829346745856 1106 18-19 0.27429660435670655 1106 20-21 0.40621747623358573 1106 22-23 0.26620120319134344 1106 24-25 0.2481178149672374 1106 26-27 0.3036392965118466 1106 28-29 0.27013538051477326 1106 30-31 0.45739942296514613 1106 32-33 0.48569505673321345 1106 34-35 0.4343770325199401 1106 36-37 0.53748895801656 1106 38-39 0.45941786353361636 1106 40-41 0.33824646864361796 1106 42-43 0.24275454342830471 1106 44-45 0.2924614257519593 1106 46-47 0.34203160106487474 1106 48-49 0.720056441873524 1106 50-51 0.44096750143625485 1106 52-53 0.5848975292899823 1106 54-55 0.2877976196733485 1106 56-57 0.4217479002405753 1106 58-59 0.5129793390368746 1106 60-61 0.42765701611430984 1106 62-63 0.3321000078039802 1106 64-65 0.6425006676520226 1106 66-67 0.04151210729268229 1106 68-69 0.1305548996251531 1106 70-71 0.4397586548609951 1106 72-73 0.4993747833764317 1106 74-75 0.4902860967910314 1106 76 0.09762394462828894 1107 1 -0.1659166640598997 1107 2 -0.9105605635734868 1107 3 -0.6401441753289809 1107 4 -1.0315559803832528 1107 5 -0.618191067694422 1107 6 -1.2760377358893962 1107 7 -0.5376365367956737 1107 8 -0.9799446990745935 1107 9 -0.2288243111719197 1107 10-11 -0.828063956506881 1107 12-13 -0.4206992078900953 1107 14-15 -0.41571828457145443 1107 16-17 -0.32225536090031426 1107 18-19 -0.4336681632594832 1107 20-21 -0.5346839480542727 1107 22-23 -0.3868722600770269 1107 24-25 -0.46961082071491944 1107 26-27 -0.55152374696641 1107 28-29 0.07359302669168244 1107 30-31 -0.7263430807829891 1107 32-33 -0.7703206853957241 1107 34-35 -0.33230212789985814 1107 36-37 -0.8178052698694884 1107 38-39 -0.6791222414139142 1107 40-41 -0.1638243709365952 1107 42-43 -0.3975378103947804 1107 44-45 -0.3721187841430975 1107 46-47 -0.20873676475221714 1107 48-49 -0.43297954013547013 1107 50-51 -0.18313294833885152 1107 52-53 -0.23111633877598337 1107 54-55 -0.23224548377492482 1107 56-57 -0.3151055480352838 1107 58-59 -0.4221780822524863 1107 60-61 -0.5242732687432579 1107 62-63 -0.6652107118361954 1107 64-65 -0.5993752693794718 1107 66-67 -0.5409091820626344 1107 68-69 -0.6513322817841356 1107 70-71 -0.5088826494868357 1107 72-73 -0.5747069257690001 1107 74-75 -0.5040636033588939 1107 76 -0.5089727570208851 1108 1 -0.6840966896933409 1108 2 -0.328336369548051 1108 3 -0.21279033524222157 1108 4 0.2727770571795993 1108 5 0.2709788031524667 1108 6 0.19826966823167425 1108 7 -0.13684781551005898 1108 8 -0.567961065041267 1108 9 -0.3181962918482526 1108 10-11 -0.1411518003400687 1108 12-13 -0.17128828410028518 1108 14-15 0.08837567185169348 1108 16-17 0.08328047662310212 1108 18-19 0.23729703441379257 1108 20-21 0.06991528294246763 1108 22-23 0.03546580558402468 1108 24-25 -0.09262422897677425 1108 26-27 -0.025513197819222455 1108 28-29 0.13100947153048992 1108 30-31 0.07701637894293611 1108 32-33 -0.16688235354124004 1108 34-35 -0.07835852147177746 1108 36-37 -0.26353878066511527 1108 38-39 -0.15382154142377402 1108 40-41 0.008932702907436862 1108 42-43 0.045026026734277025 1108 44-45 -0.28421579686122556 1108 46-47 -0.03587617123945108 1108 48-49 0.18313995113930304 1108 50-51 0.05067115301183378 1108 52-53 0.013510497465759386 1108 54-55 -0.0665425359291234 1108 56-57 0.25003050638648006 1108 58-59 -0.242670047154391 1108 60-61 -0.1153829010019578 1108 62-63 -0.011464285729523738 1108 64-65 -0.1857575034325052 1108 66-67 0.055107775445002005 1108 68-69 -0.07146685735349934 1108 70-71 -0.14222830659025476 1108 72-73 0.06298902217540103 1108 74-75 0.1355696412433005 1108 76 0.018376153557838393 1109 1 0.3069982030098757 1109 2 0.6778356356778801 1109 3 0.092310954721885 1109 4 0.055405340280916704 1109 5 0.2649119172369545 1109 6 0.7777774095180305 1109 7 0.6711742895792199 1109 8 0.6513778846666511 1109 9 0.7818772916690406 1109 10-11 0.7354306207078167 1109 12-13 0.3360024909293742 1109 14-15 0.33217738679815056 1109 16-17 0.5495221609347922 1109 18-19 0.8563596705057535 1109 20-21 0.6537554415224633 1109 22-23 0.7131925254110243 1109 24-25 0.48586235329410243 1109 26-27 0.6446299146133896 1109 28-29 0.22681070593154118 1109 30-31 0.3742111950592246 1109 32-33 0.4382669909647703 1109 34-35 0.3602067501114732 1109 36-37 0.001181677054397312 1109 38-39 0.5968279457441596 1109 40-41 0.5239647642971121 1109 42-43 0.5725672860636095 1109 44-45 0.6849692281421582 1109 46-47 0.31201618658668195 1109 48-49 0.49358730889034774 1109 50-51 0.3871880997463748 1109 52-53 0.3599912531234324 1109 54-55 0.6495977833332418 1109 56-57 0.522477233421661 1109 58-59 0.8425066283723339 1109 60-61 0.7547722250661266 1109 62-63 0.7399961218330589 1109 64-65 0.3003967816810942 1109 66-67 0.5740442683644602 1109 68-69 0.6601635431510715 1109 70-71 0.8061355864283257 1109 72-73 0.5253938516568581 1109 74-75 0.6233382586240239 1109 76 0.3915647225836807 1110 1 -0.48887920876157764 1110 2 -0.1591959861219543 1110 3 0.11106041717995652 1110 4 -0.09858967132998941 1110 5 0.07875039663834826 1110 6 -0.05518322807836995 1110 7 -0.10046356484009067 1110 8 -0.18401960967123898 1110 9 -0.21141418506613974 1110 10-11 0.052107321490481695 1110 12-13 0.009241503572354759 1110 14-15 -0.2719624027720968 1110 16-17 -0.10451585403037456 1110 18-19 -0.3627854228115268 1110 20-21 -0.1525646181088547 1110 22-23 -0.3495063651024424 1110 24-25 -0.013413756909159247 1110 26-27 0.059726958849509515 1110 28-29 0.10194329960717852 1110 30-31 0.21224622845849694 1110 32-33 -0.1314772824218835 1110 34-35 -0.051199172883855226 1110 36-37 0.1300913986793475 1110 38-39 -0.02838348741428831 1110 40-41 0.06124150521623761 1110 42-43 -0.04949056778232119 1110 44-45 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0.08811669423623414 2116 4 -0.5413385890789044 2116 5 -0.6906218977339478 2116 6 -0.09273734063248185 2116 7 0.21522907585254814 2116 8 -0.03715813780976873 2116 9 -0.2002669989189556 2116 10-11 0.13127398815714741 2116 12-13 -0.04530395097309281 2116 14-15 -1.5504346474415343E-4 2116 16-17 -0.0744292739437924 2116 18-19 -0.20861875614485825 2116 20-21 0.020108542064299684 2116 22-23 0.2696494790534061 2116 24-25 -0.2971799906753958 2116 26-27 -0.1105652489426987 2116 28-29 -0.3206757480118725 2116 30-31 -0.02471264600037415 2116 32-33 -0.05885823480283392 2116 34-35 -0.2270649737496555 2116 36-37 -0.2673111987232417 2116 38-39 -0.1311973402281339 2116 40-41 -0.6031524341777015 2116 42-43 -0.2770338578256144 2116 44-45 -0.3967235272260936 2116 46-47 -0.07869723906051718 2116 48-49 -0.17285108163744667 2116 50-51 0.16138286193783102 2116 52-53 -0.10987127948744302 2116 54-55 -0.0015636655931032806 2116 56-57 -0.24806009348983338 2116 58-59 0.26290096913091077 2116 60-61 -0.15490567981045444 2116 62-63 -0.08284905175715096 2116 64-65 -0.2644938464545632 2116 66-67 -0.17890325320888678 2116 68-69 0.11462621623957148 2116 70-71 0.1925995639519016 2116 72-73 -0.04031957399034525 2116 74-75 -0.4310892950584915 2116 76 0.09715372519255538 2117 1 0.09685262697026076 2117 2 -0.3701179470439122 2117 3 0.10883741495695531 2117 4 0.21339114065082398 2117 5 0.0036655715535225397 2117 6 0.4643265414314044 2117 7 -0.005634970011499263 2117 8 -0.07620400185563625 2117 9 -0.2566347302866845 2117 10-11 0.20433909872225797 2117 12-13 -0.3352507159198552 2117 14-15 0.04526878945909374 2117 16-17 -0.33411395862847826 2117 18-19 -0.11380917383527844 2117 20-21 -0.3022808928251308 2117 22-23 -0.097692863288934 2117 24-25 -0.12187286536826747 2117 26-27 -0.17806115393860011 2117 28-29 -0.15114257847869794 2117 30-31 0.0022017684140394067 2117 32-33 0.13583873489412923 2117 34-35 0.17455255286787263 2117 36-37 -0.07777393418598422 2117 38-39 0.1730614640306598 2117 40-41 0.0741407681155053 2117 42-43 -0.1585346768264344 2117 44-45 0.22932069881812112 2117 46-47 0.033997273633993075 2117 48-49 0.1874376161512501 2117 50-51 0.1010327390877066 2117 52-53 0.15622208660591497 2117 54-55 0.44410586757643244 2117 56-57 0.12498454205479703 2117 58-59 0.22950621073615451 2117 60-61 0.29491004500527396 2117 62-63 0.09040033399223546 2117 64-65 0.11814333618261941 2117 66-67 0.14946488765926347 2117 68-69 0.10951769863105909 2117 70-71 0.23508523143757287 2117 72-73 -0.8945169531877184 2117 74-75 -0.12571460218379826 2117 76 0.0026410306798609895 2118 1 0.21187836699600027 2118 2 -0.22034317226913913 2118 3 0.12695597995051955 2118 4 -0.014106446221763491 2118 5 0.009215764603716536 2118 6 2.821398869983227E-4 2118 7 0.19485569922917279 2118 8 0.2693759530993205 2118 9 0.6612183906914311 2118 10-11 -0.17925766768701124 2118 12-13 0.184667639935995 2118 14-15 0.3778279110807148 2118 16-17 0.32824929748477416 2118 18-19 0.03622702307591652 2118 20-21 0.30630984076559997 2118 22-23 0.1910780504819769 2118 24-25 0.48557163270122317 2118 26-27 0.4364517965118466 2118 28-29 0.26871142731280884 2118 30-31 0.07104628257105361 2118 32-33 0.08375540156080064 2118 34-35 0.24868583793865895 2118 36-37 0.3952090934845458 2118 38-39 0.37144064678484057 2118 40-41 0.3286251632248991 2118 42-43 0.4143216616549097 2118 44-45 0.048426635111560756 2118 46-47 -0.39326704425532455 2118 48-49 0.10490865862229981 2118 50-51 0.3654210112884755 2118 52-53 0.3394387854476193 2118 54-55 0.028022373367939224 2118 56-57 0.05386766625041872 2118 58-59 -0.029854712687267693 2118 60-61 0.21772013187785433 2118 62-63 0.18019883785323998 2118 64-65 0.3778762834155671 2118 66-67 0.38268359744046876 2118 68-69 0.34445576169412107 2118 70-71 0.16966936914671038 2118 72-73 0.19511062081485875 2118 74-75 0.1485373283181275 2118 76 0.407583919997748 2119 1 0.3860573489336012 2119 2 0.15235797715614652 2119 3 -0.26705571955686835 2119 4 -0.35312019827430774 2119 5 0.21436765294525983 2119 6 0.3608937983434899 2119 7 0.36081423781701716 2119 8 0.35691187263955015 2119 9 -0.2576598933808185 2119 10-11 0.1823058585855648 2119 12-13 0.26457065717737294 2119 14-15 0.5234996064830142 2119 16-17 0.10430635824010892 2119 18-19 0.10116442044866147 2119 20-21 -0.3266129835365277 2119 22-23 -0.5019022450845227 2119 24-25 0.1303017230132184 2119 26-27 0.2881938942129949 2119 28-29 0.21158652994006388 2119 30-31 -0.23692528967853832 2119 32-33 -0.2860003455656326 2119 34-35 -0.07050802495133013 2119 36-37 0.054945854568288155 2119 38-39 -0.09927466520201733 2119 40-41 -0.07935410606902593 2119 42-43 0.06057063538233365 2119 44-45 0.16401314988988958 2119 46-47 -0.40725000813052503 2119 48-49 0.0088495453218016 2119 50-51 -0.2912164066097134 2119 52-53 0.01873373618653318 2119 54-55 -0.09172824239561095 2119 56-57 -0.4889561227479291 2119 58-59 -0.24794020119301763 2119 60-61 -0.44949815629948375 2119 62-63 -0.05848907265578873 2119 64-65 -0.1486631254514279 2119 66-67 -0.4122235248912247 2119 68-69 -0.5098905026736986 2119 70-71 -0.2883304255987724 2119 72-73 0.007277082227005849 2119 74-75 -0.20231447792161106 2119 76 0.02980785267426711 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 10.0 13 221.0 14 967.0 15 2004.0 16 2787.0 17 3298.0 18 3382.0 19 2985.0 20 2247.0 21 1762.0 22 1353.0 23 1167.0 24 963.0 25 990.0 26 1111.0 27 1280.0 28 1583.0 29 1830.0 30 2375.0 31 2954.0 32 3817.0 33 5128.0 34 7197.0 35 11112.0 36 21322.0 37 86117.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.571927842694244 20.645203045386616 11.901483861098363 29.881385250820774 2 19.16957908238312 20.173332862639885 34.67069109565667 25.986396959320317 3 18.47824807898236 22.376178204539837 30.387380708628985 28.758193007848813 4 14.655040538473305 20.459867499794072 32.74849672279686 32.13659523893576 5 15.77529094738824 33.07504030312658 32.833809910450576 18.315858839034608 6 27.931537637824928 33.07445193631517 18.201715677622058 20.79229474823784 7 26.895423682940894 28.69759122627411 21.447147009331495 22.9598380814535 8 23.649403984420047 33.85639142867229 19.591438086160437 22.902766500747227 9 23.405231757687012 18.022263800143563 21.308880808651345 37.26362363351808 10-11 25.6601475623963 24.496652192843108 25.054718113460655 24.788482131299936 12-13 24.81907720549299 21.738682764382627 24.847907179251834 28.59433285087255 14-15 23.621750744284018 22.711841470446334 23.250491286287524 30.415916498982128 16-17 24.5678445770231 24.62432779091797 23.968592979607205 26.839234652451726 18-19 23.557912945246585 23.829150045304246 25.1685670914675 27.444369917981664 20-21 23.375813417116767 24.33867570398089 25.435685623845327 26.849825255057013 22-23 25.05030536237512 24.095974394276368 24.196290935620905 26.65742930772761 24-25 24.05956643122119 24.84003730256558 23.881584947179213 27.218811319034014 26-27 23.068097574752002 25.40185453218955 25.263588331509396 26.266459561549055 28-29 23.90387263035267 24.48282557277509 26.26175262705781 25.35154916981443 30-31 23.770607547569455 24.050081782986783 25.96109718642991 26.21821348301385 32-33 22.911592002918297 24.994998882103058 25.96345065367553 26.129958461303115 34-35 23.470246290347255 24.65933561619656 26.1046586884127 25.765759405043482 36-37 23.775314482060697 23.646462150363025 26.09818665348725 26.480036714089035 38-39 22.645944387568985 23.78413998423177 27.87770207458138 25.692213553617865 40-41 22.55298243136701 23.288146762217437 28.151881008696062 26.006989797719488 42-43 21.45214812722844 23.37728433414528 28.074804956402023 27.095762582224264 44-45 20.709923394641155 23.241077417305046 29.160341723444066 26.88865746460974 46-47 20.925033022184437 23.791564560209224 29.150131059092793 26.133271358513543 48-49 20.6913898400819 24.1239218178181 28.957060990103674 26.22762735199633 50-51 20.280121438909873 25.020004471587765 27.910944799425753 26.788929290076606 52-53 19.53701415610548 25.051776279403633 28.397818335863313 27.013391228627576 54-55 19.437874348383758 24.937927301396783 28.275143855685386 27.34905449453407 56-57 20.0191807580518 24.818194655275885 27.635000764876853 27.527623821795462 58-59 20.243054329791367 24.815841188030266 27.124592555983103 27.81651192619527 60-61 20.06242571869006 23.763252962426897 27.751203210129322 28.42311810875372 62-63 20.23422882762029 24.202468787140656 27.242854285075484 28.320448100163564 64-65 20.659029665454632 24.62315105729516 26.139372330285592 28.578446946964615 66-67 20.331603534907803 25.484814252597637 25.219755004059728 28.96382720843483 68-69 20.421329473647052 26.229098269024842 25.138560384085856 28.211011873242253 70-71 20.663148233134464 26.675080312069753 24.650510114025487 28.01126134077029 72-73 20.340134853673174 26.954260364081385 24.79495416622539 27.91065061602005 74-75 20.260116967322105 28.052741200974335 25.028829973758842 26.658311857944717 76 20.857137813969086 27.867569618912576 24.60093786221545 26.674354704902893 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 2.0 14 5.5 15 7.5 16 12.0 17 17.0 18 15.5 19 24.5 20 44.0 21 53.0 22 63.5 23 103.5 24 158.0 25 183.0 26 287.5 27 459.0 28 643.5 29 761.0 30 962.5 31 1376.5 32 1888.5 33 2188.0 34 2540.5 35 3357.0 36 3998.0 37 4175.0 38 4806.5 39 6216.5 40 7617.5 41 8533.5 42 8827.0 43 9242.0 44 10003.0 45 10418.5 46 10488.0 47 10414.0 48 9943.0 49 8918.0 50 8290.0 51 7747.5 52 6572.0 53 5481.5 54 5024.0 55 4887.5 56 4383.5 57 3853.5 58 3691.0 59 3610.0 60 3445.0 61 3388.0 62 3415.0 63 3358.0 64 3228.5 65 3153.5 66 3061.0 67 2971.0 68 2809.5 69 2509.5 70 2138.0 71 1905.0 72 1673.0 73 1163.5 74 697.0 75 508.0 76 364.0 77 148.5 78 56.0 79 35.0 80 28.5 81 15.5 82 11.5 83 14.0 84 11.5 85 8.5 86 8.0 87 8.0 88 7.5 89 4.5 90 4.0 91 6.5 92 7.0 93 5.0 94 5.5 95 6.0 96 4.0 97 5.0 98 6.0 99 19.5 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.941834057024512E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 2.941834057024512E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 5.883668114049024E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 169962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.922641531636486 #Duplication Level Percentage of deduplicated Percentage of total 1 72.59951117318437 39.14757416363658 2 13.309619413407821 14.353796731033997 3 5.204696229050279 8.41952907120415 4 2.4550453910614523 5.295301302644121 5 1.4522957402234637 3.9155811298996244 6 1.0714909217877095 3.466657252797684 7 0.7583362430167597 2.8624045374848497 8 0.5728439245810055 2.4711406079005895 9 0.4604574022346369 2.234617149715819 >10 2.1080656424581004 17.571574822607406 >50 0.007637918994413408 0.2618232310751815 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0035302008684294137 0.0 2 0.0 0.0 0.0 0.005883668114049023 0.0 3 0.0 0.0 0.0 0.007060401736858827 0.0 4 0.0 0.0 0.0 0.03530200868429414 0.0 5 0.0 0.0 0.0 0.042950777232557864 0.0 6 0.0 0.0 0.0 0.04412751085536767 0.0 7 0.0 0.0 0.0 0.04648097810098728 0.0 8 0.0 0.0 0.0 0.04648097810098728 0.0 9 0.0 0.0 0.0 0.0505995457808216 0.0 10 0.0 0.0 0.0 0.05765994751768042 0.0 11 0.0 0.0 0.0 0.06001341476330003 0.0 12 0.0 0.0 0.0 0.06119014838610984 0.0 13 0.0 0.0 0.0 0.06236688200891964 0.0 14 0.0 0.0 0.0 0.06530871606594416 0.0 15 0.0 0.0 0.0 0.06707381650015885 0.0 16 0.0 0.0 0.0 0.06766218331156376 0.0 17 0.0 0.0 0.0 0.06883891693437356 0.0 18 0.0 0.0 0.0 0.0758993186712324 0.0 19 0.0 0.0 0.0 0.08295972040809121 0.0 20 0.0 0.0 0.0 0.08648992127652064 0.0 21 0.0 0.0 0.0 0.09002012214495005 0.0 22 0.0 0.0 0.0 0.09531542344759417 0.0 23 0.0 0.0 0.0 0.09766889069321377 0.0 24 0.0 0.0 0.0 0.10178745837304809 0.0 25 0.0 0.0 0.0 0.10943622692131182 0.0 26 0.0 0.0 0.0 0.126498864452054 0.0 27 0.0 0.0 0.0 0.20592838399171579 0.0 28 0.0 0.0 0.0 0.2777091349831139 0.0 29 0.0 0.0 0.0 0.37067109118508845 0.0 30 0.0 0.0 0.0 0.475400383615161 0.0 31 0.0 0.0 0.0 0.5977806803873807 0.0 32 0.0 0.0 0.0 0.7348701474447229 0.0 33 0.0 0.0 0.0 0.9360915969451995 0.0 34 0.0 0.0 0.0 1.2196844000423623 0.0 35 0.0 0.0 0.0 1.601534460644144 0.0 36 0.0 0.0 0.0 2.1098833856979797 0.0 37 0.0 0.0 0.0 2.9112389828314567 0.0 38 0.0 0.0 0.0 3.6208093573857685 0.0 39 0.0 0.0 0.0 4.36391664019016 0.0 40 0.0 0.0 0.0 5.209399748179004 0.0 41 0.0 0.0 0.0 6.052529388922229 0.0 42 0.0 0.0 0.0 6.949200409503301 0.0 43 0.0 0.0 0.0 7.879408338334451 0.0 44 0.0 0.0 0.0 8.786081594709406 0.0 45 0.0 0.0 0.0 9.763358868452949 0.0 46 0.0 0.0 0.0 10.748873277556159 0.0 47 0.0 0.0 0.0 11.637307162777562 0.0 48 0.0 0.0 0.0 12.397477083112696 0.0 49 0.0 0.0 0.0 13.095868488250314 0.0 50 0.0 0.0 0.0 13.796024993822149 0.0 51 0.0 0.0 0.0 14.517362704604558 0.0 52 0.0 0.0 0.0 15.292241795224815 0.0 53 0.0 0.0 0.0 16.087713724244242 0.0 54 0.0 0.0 0.0 16.852590579070615 0.0 55 0.0 0.0 0.0 17.54450994928278 0.0 56 0.0 0.0 0.0 18.273496428613456 0.0 57 0.0 0.0 0.0 18.953060095786118 0.0 58 0.0 0.0 0.0 19.628505195278944 0.0 59 0.0 0.0 0.0 20.281592355938386 0.0 60 0.0 0.0 0.0 20.983513961944436 0.0 61 0.0 0.0 0.0 21.65601722738024 0.0 62 0.0 0.0 0.0 22.291453383697533 0.0 63 0.0 0.0 0.0 22.90217813393582 0.0 64 0.0 0.0 0.0 23.56467916357774 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGGTC 15 0.0022216348 70.0 9 AGGCATC 15 0.0022216348 70.0 8 CGGTACG 15 0.0022216348 70.0 14 CGCAACT 15 0.0022216348 70.0 68 GCCGGTA 20 0.0069418997 52.5 12 CAACTTG 20 0.0069418997 52.5 70 GTTATAG 20 0.0069418997 52.5 2 GGATAAA 30 5.8651954E-4 46.666664 32 TACAATT 30 5.8651954E-4 46.666664 7 GGACTAG 40 4.5782173E-5 43.75 1 GCGAAAT 35 0.0012527524 40.0 11 GGCACGA 35 0.0012527524 40.0 27 TTAGATA 35 0.0012527524 40.0 2 GGCAACT 35 0.0012527524 40.0 30 CTCCAAT 35 0.0012527524 40.0 12 GGCGAAA 35 0.0012527524 40.0 10 CATCGTT 45 9.1688424E-5 38.888885 32 GACTAGG 45 9.1688424E-5 38.888885 2 AGTCATC 40 0.0024136568 35.0 8 GAGTCAT 40 0.0024136568 35.0 41 >>END_MODULE