FastQCFastQC Report
Wed 25 May 2016
SRR1780222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169962
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT91715.395912027394359No Hit
CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG27141.5968275261529048RNA PCR Primer, Index 47 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC26901.582706722679187No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG23511.3832503736129254No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5440.32007154540426686No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT2880.16944964168461185No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2760.16238923994775303Illumina PCR Primer Index 9 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGTT150.002221634870.09
CAAGTTG150.002221634870.010
ATCGTAT150.002221634870.039
ATATCGT150.002221634870.037
AGTTGCG150.002221634870.012
CGTACCG150.002221634870.066
CATCGTT207.9092235E-570.032
TCGTTGT200.006941899752.534
TGCCCAT200.006941899752.512
CAGTTTC200.006941899752.54
CACGCTA200.006941899752.523
AAGCGCA200.006941899752.55
CTGGTCG200.006941899752.544
CGGATGG200.006941899752.529
GGTATTG200.006941899752.533
TTGCGGG200.006941899752.514
GAACGGA200.006941899752.526
AACTACA200.006941899752.546
ATGCCCA200.006941899752.511
CTTTATA950.044.210532
Produced by FastQC (version 0.11.5)