##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780222_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 169962 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.73639401748626 34.0 34.0 34.0 34.0 34.0 2 33.7308810204634 34.0 34.0 34.0 34.0 34.0 3 33.76118779491886 34.0 34.0 34.0 34.0 34.0 4 33.78739365269884 34.0 34.0 34.0 34.0 34.0 5 33.78953530789235 34.0 34.0 34.0 34.0 34.0 6 37.559413280615665 38.0 38.0 38.0 37.0 38.0 7 37.56958025911674 38.0 38.0 38.0 37.0 38.0 8 37.475824007719375 38.0 38.0 38.0 37.0 38.0 9 37.48867982254857 38.0 38.0 38.0 37.0 38.0 10-11 37.44548487308928 38.0 38.0 38.0 37.0 38.0 12-13 37.435100198867985 38.0 38.0 38.0 37.0 38.0 14-15 37.36268401172027 38.0 38.0 38.0 37.0 38.0 16-17 37.3942528329862 38.0 38.0 38.0 37.0 38.0 18-19 37.36218978359869 38.0 38.0 38.0 36.5 38.0 20-21 37.383273908285375 38.0 38.0 38.0 37.0 38.0 22-23 37.37619291371013 38.0 38.0 38.0 37.0 38.0 24-25 37.381602946540994 38.0 38.0 38.0 37.0 38.0 26-27 37.29819018368812 38.0 38.0 38.0 36.5 38.0 28-29 37.28159529777244 38.0 38.0 38.0 36.5 38.0 30-31 37.271313587743144 38.0 38.0 38.0 36.5 38.0 32-33 37.181275814593846 38.0 38.0 38.0 36.0 38.0 34-35 37.226356479683695 38.0 38.0 38.0 36.0 38.0 36-37 37.22757734081736 38.0 38.0 38.0 36.0 38.0 38-39 36.70421329473647 38.0 38.0 38.0 34.0 38.0 40-41 36.96141490450807 38.0 38.0 38.0 35.5 38.0 42-43 37.17213553617867 38.0 38.0 38.0 36.0 38.0 44-45 37.23393758604865 38.0 38.0 38.0 36.0 38.0 46-47 37.18086101599181 38.0 38.0 38.0 36.0 38.0 48-49 37.20610783586919 38.0 38.0 38.0 36.0 38.0 50-51 37.09197349995881 38.0 38.0 38.0 36.0 38.0 52-53 37.09223532318989 38.0 38.0 38.0 36.0 38.0 54-55 37.06125486873536 38.0 38.0 38.0 36.0 38.0 56-57 37.10344959461527 38.0 38.0 38.0 36.0 38.0 58-59 37.01555053482543 38.0 38.0 38.0 36.0 38.0 60-61 37.01383544557018 38.0 38.0 38.0 36.0 38.0 62-63 36.99840258410703 38.0 38.0 38.0 36.0 38.0 64-65 36.90376378249256 38.0 38.0 38.0 35.5 38.0 66-67 36.611063061154844 38.0 38.0 38.0 34.5 38.0 68-69 36.53770842894294 38.0 38.0 38.0 34.0 38.0 70-71 35.974435462044454 38.0 37.5 38.0 32.0 38.0 72-73 35.03216012991139 38.0 37.0 38.0 24.0 38.0 74-75 34.75367435073722 38.0 37.0 38.0 23.0 38.0 76 34.150763111754394 38.0 35.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01354606184795415 1101 2 0.04027616168028203 1101 3 0.05251860603539171 1101 4 0.003289635151183745 1101 5 -0.018783797672419666 1101 6 -0.164128557017321 1101 7 0.009879747996961896 1101 8 -0.09126462054187101 1101 9 -0.13557971953185444 1101 10-11 0.16012122798939288 1101 12-13 -0.04805055989642426 1101 14-15 0.16224181173666352 1101 16-17 0.12645846936563743 1101 18-19 0.15023466377623151 1101 20-21 0.004144884466221299 1101 22-23 0.06114580519273716 1101 24-25 0.07788505103867038 1101 26-27 -0.010024680461867774 1101 28-29 -0.16403397472765135 1101 30-31 -0.044096059848584446 1101 32-33 0.03367420558870293 1101 34-35 0.15306579208031934 1101 36-37 0.21092034264962223 1101 38-39 0.3500572409854854 1101 40-41 0.19138098023785943 1101 42-43 0.23550359900748674 1101 44-45 0.2943576941771937 1101 46-47 0.23196842630121495 1101 48-49 0.07711224478467926 1101 50-51 0.11814253905301086 1101 52-53 0.28134499647190836 1101 54-55 0.19486706386818753 1101 56-57 0.1690991866918452 1101 58-59 0.17477427275048285 1101 60-61 0.12506156273219204 1101 62-63 0.21113825735437786 1101 64-65 -0.003176181301341785 1101 66-67 0.08969892226207321 1101 68-69 -0.1601704286827328 1101 70-71 0.09517888288716847 1101 72-73 0.3028076296685285 1101 74-75 0.23365556591267733 1101 76 -0.3253255722142967 1102 1 -0.22225537730107447 1102 2 -0.092970266518698 1102 3 -0.016956834573640833 1102 4 -0.06991925002974853 1102 5 -0.08019791801864784 1102 6 0.050304647696414406 1102 7 -0.13429586989854414 1102 8 -0.04308441405527219 1102 9 -0.48217477636781325 1102 10-11 -0.1210342626769858 1102 12-13 -0.03628186660202459 1102 14-15 -0.16553480730598835 1102 16-17 -0.0841422189230272 1102 18-19 -0.12015328074352993 1102 20-21 -0.018255782965212575 1102 22-23 -0.16447209031252896 1102 24-25 -0.1212024450505993 1102 26-27 -0.14240031921460883 1102 28-29 -0.06540012343971568 1102 30-31 0.045303251862748084 1102 32-33 7.927789540147501E-4 1102 34-35 -0.09222047343224915 1102 36-37 0.07436804313977063 1102 38-39 0.2080455609354246 1102 40-41 0.07921078522274172 1102 42-43 0.014166650420555982 1102 44-45 0.1004897204573112 1102 46-47 0.05394152047161782 1102 48-49 -0.04164362131188426 1102 50-51 -0.2128148079070442 1102 52-53 -0.024646243490551 1102 54-55 -0.2635331864186057 1102 56-57 -0.06911126278151158 1102 58-59 0.05221196176915299 1102 60-61 0.10473618990916833 1102 62-63 -0.2631917040597358 1102 64-65 -0.15296760897889072 1102 66-67 -0.08584597293033625 1102 68-69 0.06203209504236895 1102 70-71 0.07805509521379861 1102 72-73 -0.10291768058279871 1102 74-75 -0.004064738602913565 1102 76 0.1437118665175845 1103 1 -0.1613334688455339 1103 2 0.02479323689502877 1103 3 -0.04964984148189444 1103 4 -0.0689395707989533 1103 5 0.046887687766513864 1103 6 -0.12486603868503465 1103 7 0.14695286762821524 1103 8 -0.14238550887206003 1103 9 0.04936685494960358 1103 10-11 -0.0937547410028543 1103 12-13 -0.06262210818890424 1103 14-15 0.16651585322000528 1103 16-17 0.10872329232812916 1103 18-19 -0.0166153257481767 1103 20-21 0.17251907260785515 1103 22-23 0.2126438148302796 1103 24-25 0.031981007460210265 1103 26-27 0.013686821738005506 1103 28-29 -0.06664239710352149 1103 30-31 -0.02984925490410717 1103 32-33 0.1086584922009024 1103 34-35 -0.3258992194428316 1103 36-37 -0.06394347465605676 1103 38-39 -0.0412323043738283 1103 40-41 -0.03671455716000338 1103 42-43 0.017312733723592544 1103 44-45 0.10763026907768136 1103 46-47 0.00989635422914148 1103 48-49 0.057051486351831215 1103 50-51 -0.02874621016132295 1103 52-53 0.1265576509868822 1103 54-55 0.08475287991679181 1103 56-57 0.004208132847296042 1103 58-59 0.19669444874043052 1103 60-61 0.1502710745696092 1103 62-63 -0.08541527292008055 1103 64-65 0.13191705146630994 1103 66-67 -0.40890782401909576 1103 68-69 -0.3001714762419212 1103 70-71 -0.4508403922018758 1103 72-73 -0.3159070152089072 1103 74-75 -0.32354116570265745 1103 76 -0.7621682288243932 1104 1 0.04184499051270052 1104 2 -0.021861282793196324 1104 3 -0.05573851442449751 1104 4 0.028322297811236297 1104 5 0.04499984486743358 1104 6 -0.0471962340489398 1104 7 -0.03668414016609489 1104 8 -0.0757694546155605 1104 9 0.11558307116582256 1104 10-11 -0.016975714785615992 1104 12-13 0.1252967095051929 1104 14-15 -0.04932076991998713 1104 16-17 -0.018263244770764686 1104 18-19 0.13880273275977117 1104 20-21 -0.045554619061348944 1104 22-23 -0.02577606572442903 1104 24-25 -0.0723109249216165 1104 26-27 -0.103857970244988 1104 28-29 0.00589024790103565 1104 30-31 -0.1348851897153267 1104 32-33 0.007975224663702818 1104 34-35 0.07434878136696454 1104 36-37 -0.0980030960752245 1104 38-39 -0.19665036288177618 1104 40-41 -0.01176770019067419 1104 42-43 -0.06628631476309721 1104 44-45 -0.01898173618601362 1104 46-47 -0.20286303437293896 1104 48-49 -0.13004741029762812 1104 50-51 -0.1917738445372663 1104 52-53 -0.12224789613326692 1104 54-55 -0.04371407017937656 1104 56-57 -0.21961259799590493 1104 58-59 -0.034136100910345135 1104 60-61 0.048800784054371604 1104 62-63 -0.0919189676260217 1104 64-65 -0.006350991857914323 1104 66-67 -0.3104970536982137 1104 68-69 -0.2714717090590071 1104 70-71 -0.25433770974413505 1104 72-73 -0.5400719104412204 1104 74-75 -0.6347923044818842 1104 76 -0.21719910239459495 1105 1 0.050466566483912345 1105 2 -0.06544405922461749 1105 3 -0.07065614189995983 1105 4 -0.011808910727111765 1105 5 0.11093703122762122 1105 6 0.15932992198151652 1105 7 0.12129485667608719 1105 8 -0.03636772638368768 1105 9 0.18224973783248544 1105 10-11 0.03805353786425769 1105 12-13 0.16038030598998176 1105 14-15 0.04841245570484887 1105 16-17 0.04960535085724871 1105 18-19 0.12045556867565921 1105 20-21 0.0981358658717042 1105 22-23 0.08093858018386868 1105 24-25 -0.05439949068499317 1105 26-27 0.18773587078705134 1105 28-29 0.11888136194965426 1105 30-31 -0.030099505154360884 1105 32-33 0.1314018237677672 1105 34-35 0.13522366815064402 1105 36-37 0.1411807672328962 1105 38-39 -0.14998075871051952 1105 40-41 -0.035581070732398246 1105 42-43 -0.04918759012967655 1105 44-45 -0.020115123373599886 1105 46-47 -0.11465466738070518 1105 48-49 0.10414269520774866 1105 50-51 -0.018309302665585392 1105 52-53 0.20765345267091817 1105 54-55 0.04223241827867952 1105 56-57 0.03470186187043822 1105 58-59 0.20803667331794884 1105 60-61 0.07115519398313808 1105 62-63 0.17730332803381543 1105 64-65 -0.10542322999162224 1105 66-67 -0.3405159026859934 1105 68-69 0.0028153340978320784 1105 70-71 -0.016950620076805478 1105 72-73 0.27820915978961835 1105 74-75 -0.06014541701279086 1105 76 -0.04393529000321905 1106 1 -0.04217767512077586 1106 2 -0.0640542312627872 1106 3 -0.01988516301032206 1106 4 0.08974713314637484 1106 5 -0.05613343680634131 1106 6 -0.04887194113921112 1106 7 -0.09306732497817904 1106 8 -0.08444826127963978 1106 9 -0.025365204696250032 1106 10-11 -0.06932945364653165 1106 12-13 0.08495866223670134 1106 14-15 -0.06612627166349228 1106 16-17 0.02751600745705929 1106 18-19 0.05713622752924152 1106 20-21 0.06399352119436941 1106 22-23 -0.06847027339587441 1106 24-25 -0.01585993693246479 1106 26-27 -0.08792664517719118 1106 28-29 0.13818635406144608 1106 30-31 -0.03700403899854621 1106 32-33 -0.012205906680740952 1106 34-35 0.08941803746925103 1106 36-37 0.08448189036414533 1106 38-39 -0.06325971972021449 1106 40-41 0.15084369074307347 1106 42-43 0.10991719162977631 1106 44-45 0.0066672370801583725 1106 46-47 0.06903739181845481 1106 48-49 0.003861442860063846 1106 50-51 0.1093013201275923 1106 52-53 0.05679607907014628 1106 54-55 0.010710087123996459 1106 56-57 0.06562083865655666 1106 58-59 -0.085400145854166 1106 60-61 0.11237110563515529 1106 62-63 0.03552678983233193 1106 64-65 0.20824122046288807 1106 66-67 0.1953073825668028 1106 68-69 0.11238871325792132 1106 70-71 0.05212054287112977 1106 72-73 0.2927835719299523 1106 74-75 0.3636675947819654 1106 76 0.7296964807127324 1107 1 -0.07083209790938128 1107 2 -0.13429411132275249 1107 3 0.02784722079777424 1107 4 -0.04175586535437503 1107 5 -0.054071967540970434 1107 6 -0.04223775823066944 1107 7 -0.017167774753296783 1107 8 -0.08302397342357182 1107 9 0.08732220160060677 1107 10-11 0.01639018653338553 1107 12-13 0.07973002655453598 1107 14-15 0.017044642879241678 1107 16-17 0.059496892014777814 1107 18-19 -0.06427494188605465 1107 20-21 -0.10614328540233231 1107 22-23 0.032101315809519804 1107 24-25 0.13294066276768746 1107 26-27 -0.016571697650945794 1107 28-29 0.019080282097419854 1107 30-31 -0.17518807198205621 1107 32-33 0.053517481625107166 1107 34-35 -0.08619103299551512 1107 36-37 0.05561195033415345 1107 38-39 -0.18161616649681633 1107 40-41 0.14181070723483202 1107 42-43 0.18312746149484127 1107 44-45 0.05115749195272201 1107 46-47 -0.0039011389161771604 1107 48-49 -0.009566218309352337 1107 50-51 0.0033043186283450154 1107 52-53 -0.05060646965549864 1107 54-55 0.22348182625442803 1107 56-57 -0.16340839672574958 1107 58-59 -0.15333492846286134 1107 60-61 -0.31270385688358004 1107 62-63 -0.06108115619466048 1107 64-65 0.1863334243609458 1107 66-67 -0.16285416166108746 1107 68-69 -0.025514210280306315 1107 70-71 -0.29084235068209807 1107 72-73 -0.41602452402207035 1107 74-75 -0.3042109659376777 1107 76 -0.4161243588674779 1108 1 -0.15508231973723952 1108 2 0.04357732710769113 1108 3 0.06401995075932376 1108 4 0.058396949394712294 1108 5 -0.010889477153511962 1108 6 -0.05159889398932194 1108 7 0.006715691676760116 1108 8 0.014230290600877993 1108 9 0.009914136925459616 1108 10-11 -0.1867252625447975 1108 12-13 -0.158702882841105 1108 14-15 -0.07352520824962028 1108 16-17 0.04642630145971083 1108 18-19 0.07072456516314674 1108 20-21 0.08550859274022571 1108 22-23 0.013756151656906468 1108 24-25 0.051776312969799676 1108 26-27 -0.028439488245453504 1108 28-29 0.04598553695298335 1108 30-31 0.1282527246264351 1108 32-33 0.22070421138948149 1108 34-35 0.17675139035063125 1108 36-37 0.03916218202103039 1108 38-39 -0.12970598213322404 1108 40-41 0.1020517601777442 1108 42-43 0.14566812993612643 1108 44-45 0.07481173625914295 1108 46-47 0.11428135147128415 1108 48-49 0.24021934809112366 1108 50-51 0.24028302315362993 1108 52-53 -0.028418753989519985 1108 54-55 -0.08757506026829276 1108 56-57 0.049107624175363185 1108 58-59 0.05419735981476492 1108 60-61 0.1479192329320469 1108 62-63 0.12928412035074643 1108 64-65 0.15932711459065985 1108 66-67 0.18093712296871445 1108 68-69 0.08687117865787997 1108 70-71 0.29670817677526884 1108 72-73 -0.11123550696596851 1108 74-75 0.03818280316416889 1108 76 0.29557829320783924 1109 1 0.06765048199272172 1109 2 0.01849886305098636 1109 3 2.7259751339414606E-4 1109 4 0.020538980600129264 1109 5 0.03461216721284188 1109 6 -0.21080959813853184 1109 7 -0.07482685911839582 1109 8 -0.12296542653807307 1109 9 -0.02304827541254184 1109 10-11 -0.0021432619741545977 1109 12-13 0.034761699459807005 1109 14-15 -0.10153199595366402 1109 16-17 -0.026113531250409494 1109 18-19 0.1300719244159012 1109 20-21 0.09550994427422665 1109 22-23 0.030808005511026693 1109 24-25 -0.04018055807883769 1109 26-27 -0.08955135020877947 1109 28-29 -0.10859991753785891 1109 30-31 -0.06443847989068274 1109 32-33 0.15559781563029418 1109 34-35 -0.02017462067187381 1109 36-37 -0.02511076777698662 1109 38-39 0.24585527989918177 1109 40-41 -0.03249344863425563 1109 42-43 0.012204148316996566 1109 44-45 0.021358566911935384 1109 46-47 0.009672620561701706 1109 48-49 0.016585523700442195 1109 50-51 0.08289688989237476 1109 52-53 0.08689712953828632 1109 54-55 -0.044408566297633456 1109 56-57 -0.036414443371306504 1109 58-59 0.130521487699923 1109 60-61 -0.005111957469047468 1109 62-63 0.06348694055166959 1109 64-65 0.18983424017820028 1109 66-67 0.3320085890827471 1109 68-69 0.3928584742383592 1109 70-71 0.5347959063483358 1109 72-73 0.30267928632441254 1109 74-75 0.63879700027789 1109 76 1.1729340920395188 1110 1 -0.09107489858696027 1110 2 0.003791715893505909 1110 3 0.05690801507596177 1110 4 0.10220235034297076 1110 5 -0.005323614444790792 1110 6 -0.17868609250508882 1110 7 -0.09532512464977572 1110 8 -0.138934005420559 1110 9 -0.008226452643704363 1110 10-11 -0.10241274966085712 1110 12-13 -0.17034996591675622 1110 14-15 -0.11130092459676177 1110 16-17 0.023956707561545443 1110 18-19 -0.010341194474044357 1110 20-21 -0.032611382522603094 1110 22-23 -0.2445926424061753 1110 24-25 -0.03701981154060974 1110 26-27 -0.05348587043469166 1110 28-29 0.09458376412264613 1110 30-31 0.006216031161173419 1110 32-33 -0.0460449125025022 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0.21772221004135162 2115 54-55 0.3584738485918848 2115 56-57 0.3512306140440913 2115 58-59 0.2970539825862062 2115 60-61 0.2609000521710598 2115 62-63 0.3541561792660559 2115 64-65 0.22419740521455367 2115 66-67 0.41714184950764377 2115 68-69 0.24416069997099044 2115 70-71 0.2118899987395224 2115 72-73 0.3917237807246394 2115 74-75 0.4896562059809497 2115 76 0.3958820232625584 2116 1 0.07580821829615303 2116 2 0.09989561199740393 2116 3 0.027254335422284726 2116 4 0.06486468800530787 2116 5 -0.06592967505085312 2116 6 0.0881970243780259 2116 7 -0.01697014629479554 2116 8 0.12221318072662513 2116 9 -0.06396238337963212 2116 10-11 -0.028224437972546923 2116 12-13 -0.06261187067866558 2116 14-15 0.13512764683180478 2116 16-17 -0.21619480758996446 2116 18-19 -0.10444292607577665 2116 20-21 -0.12254644745766541 2116 22-23 -0.016021213834747527 2116 24-25 0.14820746118665795 2116 26-27 -0.05170015614897494 2116 28-29 -0.1827971882583057 2116 30-31 -0.00834024589509852 2116 32-33 -0.03971374367133507 2116 34-35 0.0299156863720782 2116 36-37 -0.0045659152784907064 2116 38-39 -0.035222885864406805 2116 40-41 -0.15468138762683026 2116 42-43 -0.01721838830752631 2116 44-45 -0.017666618719211158 2116 46-47 -0.14189521006241534 2116 48-49 -0.10536661356626809 2116 50-51 -0.05899805291316085 2116 52-53 -0.15100172657249544 2116 54-55 -0.2853126401487316 2116 56-57 -0.3051080014688381 2116 58-59 -0.01721832767234588 2116 60-61 -0.22219002289148904 2116 62-63 -0.24887759888240168 2116 64-65 -0.07008950054024154 2116 66-67 -0.038842303953572355 2116 68-69 -0.10491144348188897 2116 70-71 0.02921693785545898 2116 72-73 -0.35905812086001276 2116 74-75 0.17424753208604216 2116 76 -0.005452422108589872 2117 1 0.03010797259590703 2117 2 -0.029260817159027397 2117 3 -0.02262281445486991 2117 4 0.12024961839023973 2117 5 0.015356156234979323 2117 6 0.08234116852217 2117 7 0.14019611087146444 2117 8 -0.3833764998630542 2117 9 0.0020695576523053205 2117 10-11 0.11795697070886035 2117 12-13 0.006071585504791699 2117 14-15 0.056155492859652156 2117 16-17 0.14588545449029056 2117 18-19 0.22550179136894144 2117 20-21 0.10044700053578026 2117 22-23 0.002058229244696008 2117 24-25 0.035359385838582114 2117 26-27 0.11616429921548388 2117 28-29 -0.472047802508925 2117 30-31 0.1097903847355326 2117 32-33 0.07937921292161576 2117 34-35 0.2889656454220315 2117 36-37 0.07231778660521115 2117 38-39 0.1558397676982466 2117 40-41 -0.009687530132971744 2117 42-43 0.05222264363349893 2117 44-45 0.12226990871731402 2117 46-47 0.15404049837329126 2117 48-49 0.03565508995543354 2117 50-51 0.20774027632516834 2117 52-53 -0.02659550216626627 2117 54-55 0.08362675379066076 2117 56-57 0.014343267982432906 2117 58-59 0.015388142434119345 2117 60-61 0.08270350700204432 2117 62-63 0.28958472707991945 2117 64-65 0.02155669110594971 2117 66-67 0.3681192030079359 2117 68-69 0.4453240192535759 2117 70-71 0.3070424906810132 2117 72-73 0.3744083001064027 2117 74-75 0.11564802348653558 2117 76 -0.3882943549505171 2118 1 -0.0058476258596869 2118 2 -0.04227159579480144 2118 3 -0.1430378723699235 2118 4 -0.05303303926741876 2118 5 -0.005255973752149146 2118 6 0.011857738663742623 2118 7 0.10928797058831918 2118 8 0.05756220670065204 2118 9 -0.04691692883417886 2118 10-11 0.010411925663817101 2118 12-13 0.16281426568497182 2118 14-15 0.015423913065575334 2118 16-17 -0.09448214525230725 2118 18-19 -0.21383821828356986 2118 20-21 0.0549494941007751 2118 22-23 -0.1542923177308566 2118 24-25 -0.16029497387827263 2118 26-27 -0.17320989640871431 2118 28-29 0.0027953442092254477 2118 30-31 0.12759725410489864 2118 32-33 -0.06146699042458437 2118 34-35 -0.09835135908247139 2118 36-37 0.0842124938124158 2118 38-39 0.25978523101870366 2118 40-41 0.31128857990563574 2118 42-43 0.05792365714701475 2118 44-45 -0.10189957326466725 2118 46-47 0.02170056915836227 2118 48-49 -0.1193682369428899 2118 50-51 -0.0738113646014753 2118 52-53 -0.18677688891015265 2118 54-55 0.12008508712399646 2118 56-57 -0.18195459237792733 2118 58-59 0.078662354145834 2118 60-61 0.22590250957603786 2118 62-63 0.013744154364346173 2118 64-65 0.0914770579013151 2118 66-67 0.0323224687736996 2118 68-69 -0.04105186555980822 2118 70-71 -0.05067348422246454 2118 72-73 0.09924139212700567 2118 74-75 0.29246993468344584 2118 76 0.025878746722582946 2119 1 -0.1078385946610041 2119 2 -0.01922664505588756 2119 3 -0.09933918599882219 2119 4 0.0125919607325784 2119 5 -0.04377711496725567 2119 6 -0.039963895162202334 2119 7 -0.18071100313910193 2119 8 -0.08674711185435768 2119 9 -0.006256009293949205 2119 10-11 0.11544066129599884 2119 12-13 0.027918432351640377 2119 14-15 -0.2543446624680854 2119 16-17 -0.08304433737053074 2119 18-19 0.009937951667168932 2119 20-21 0.01018604992999883 2119 22-23 0.13073949671906604 2119 24-25 -0.13769901739222945 2119 26-27 0.01659921689177679 2119 28-29 0.08739612417638654 2119 30-31 -0.07084024589509852 2119 32-33 -0.04597027449683111 2119 34-35 0.14882895700948495 2119 36-37 0.01170890185839113 2119 38-39 -0.11836029443285412 2119 40-41 -0.1330643552339339 2119 42-43 -0.23598798078401728 2119 44-45 0.02735689225257687 2119 46-47 -0.2599855966872866 2119 48-49 -0.2088540743813141 2119 50-51 0.12050821667931899 2119 52-53 0.18165240090922197 2119 54-55 -0.030166349657612557 2119 56-57 0.031569114518624986 2119 58-59 -0.11025646769324737 2119 60-61 -0.1350426874682924 2119 62-63 -0.022088152696412067 2119 64-65 -0.015753032410671608 2119 66-67 0.2264137044058856 2119 68-69 -0.013832551109892677 2119 70-71 0.19630157735389275 2119 72-73 -0.1543428648516496 2119 74-75 -0.2693783822295259 2119 76 -1.1474586917010612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 2.0 19 2.0 20 4.0 21 3.0 22 10.0 23 17.0 24 28.0 25 70.0 26 112.0 27 220.0 28 421.0 29 680.0 30 1116.0 31 1811.0 32 2821.0 33 4845.0 34 7880.0 35 13854.0 36 21799.0 37 114266.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.74468410585895 17.620409267954013 12.721667196196798 32.913239429990234 2 19.39845377201963 19.6949906449677 35.96392134712465 24.942634235888022 3 18.434120568126993 19.81148727362587 32.35487932596698 29.399512832280156 4 14.227297866581942 23.126934255892493 34.17940480813358 28.46636306939198 5 14.457937656652664 31.185794471705442 38.51978677586755 15.83648109577435 6 27.95977924477236 37.888469187230086 17.57510502347584 16.57664654452172 7 26.26587119473765 26.079358915522295 23.23695884962521 24.41781104011485 8 23.02808863157647 36.57464609736294 17.524505477695016 22.872759793365578 9 22.40500817829868 16.752568221131785 19.001894541132724 41.84052905943682 10-11 25.84312964074322 26.69449641684612 24.422223791200388 23.04015015121027 12-13 24.85732104823431 20.343959237947306 26.036702321695437 28.762017392122946 14-15 22.089937750791353 20.02565279297725 21.590708511314293 36.2937009449171 16-17 26.224391334533603 25.844600557771734 21.00469516715501 26.92631294053965 18-19 27.79533072098469 24.744942987255975 21.898130170273355 25.56159612148598 20-21 23.710888316211857 23.414645626669493 21.57099822313223 31.30346783398642 22-23 22.714489121097657 25.76311175439216 22.564455584189407 28.95794354032078 24-25 27.171956084301197 26.046410374083617 22.361174850849014 24.42045869076617 26-27 26.536519927983903 27.139595909673925 22.926301173203424 23.397582989138748 28-29 23.07398124286605 25.53659053200127 25.668384697755968 25.721043527376708 30-31 26.03817323872395 22.42589520010355 27.84798955060543 23.687942010567067 32-33 23.568797731257575 22.34499476353538 27.151069062496326 26.93513844271072 34-35 22.04492768971888 28.48872100822537 25.563655405325896 23.90269589672986 36-37 26.755392381826525 27.510855367670423 23.266083006789753 22.4676692437133 38-39 22.5518056977442 24.653157764676813 27.597933655758343 25.197102881820644 40-41 21.712206257869408 21.29976112307457 28.750838422706256 28.237194196349773 42-43 25.29624268954237 21.00822536802344 28.162765794707052 25.532766147727138 44-45 21.15443452065756 25.034125275061488 30.364140219578495 23.447299984702465 46-47 23.660288770431038 22.550040597309987 28.559030842188253 25.230639790070718 48-49 23.33551029053553 21.605417681599416 25.92991374542545 29.129158282439604 50-51 21.707793506783872 22.158482484320025 28.417822807451078 27.715901201445032 52-53 18.41705793059625 22.126122309692757 34.518304091502806 24.93851566820819 54-55 18.9871853708476 22.953071863122346 30.90161330179687 27.158129464233184 56-57 21.989621209446817 23.797966604299784 26.283816382485497 27.9285958037679 58-59 19.755592426542403 23.853861451383253 30.53917934597145 25.851366776102893 60-61 22.138183829326554 26.834527717960487 26.645661971499514 24.38162648121345 62-63 19.143690942681303 32.6331768277615 23.44553488426825 24.777597345288946 64-65 18.420293948058976 34.196173262258625 22.44119273720008 24.94234005248232 66-67 18.624163048210775 33.857273978889395 22.22849813487721 25.29006483802262 68-69 18.40352549393394 34.24294842376531 22.474141278638754 24.879384803661996 70-71 18.530318541791694 32.77056047822454 23.013379461291347 25.685741518692414 72-73 18.889516480154388 30.569186053353103 23.746484508301855 26.794812958190654 74-75 18.970711100128266 29.6928136877655 24.611972087878467 26.724503124227766 76 18.863039973641165 30.254998176062887 24.473705887198317 26.40825596309763 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 4.0 16 10.0 17 14.0 18 12.5 19 15.5 20 26.5 21 33.0 22 52.0 23 92.5 24 131.0 25 148.0 26 248.5 27 411.0 28 561.0 29 649.0 30 803.0 31 1178.0 32 1598.5 33 1798.0 34 2166.5 35 2774.0 36 3377.0 37 3741.0 38 4279.5 39 7566.0 40 12019.5 41 13069.0 42 12413.0 43 11478.0 44 10404.0 45 9875.0 46 9485.0 47 9276.0 48 8783.5 49 7905.5 50 7311.0 51 6841.0 52 5758.0 53 4669.5 54 4194.0 55 4080.5 56 3656.0 57 3253.5 58 3162.0 59 3066.0 60 3020.0 61 3088.0 62 3106.0 63 3155.0 64 3213.5 65 3188.5 66 3157.5 67 3161.0 68 2970.5 69 2575.0 70 2149.5 71 1929.0 72 1658.5 73 1146.0 74 681.5 75 459.0 76 334.5 77 131.0 78 31.5 79 11.0 80 8.0 81 3.5 82 2.0 83 2.0 84 1.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 169962.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.255974864969815 #Duplication Level Percentage of deduplicated Percentage of total 1 56.767896906239635 21.149433402760618 2 20.738775445744697 15.452865934738352 3 8.464806304385592 9.46093832739083 4 3.9749846022646516 5.923677057224555 5 2.2014813411032677 4.100916675492169 6 1.4939751425277554 3.339570021534225 7 1.1354842785173955 2.961250161800873 8 0.9080715718324094 2.7064873324625505 9 0.724878002558393 2.430543297913651 >10 3.5343724830624907 20.6434379449524 >50 0.037902117780831 0.9131452913004083 >100 0.00947552944520775 0.6389663571857239 >500 0.0015792549075346253 0.32007154540426686 >1k 0.004737764722603875 4.562784622445017 >5k 0.0015792549075346253 5.395912027394359 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 9171 5.395912027394359 No Hit CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG 2714 1.5968275261529048 RNA PCR Primer, Index 47 (96% over 25bp) CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 2690 1.582706722679187 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 2351 1.3832503736129254 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 544 0.32007154540426686 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT 288 0.16944964168461185 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 276 0.16238923994775303 Illumina PCR Primer Index 9 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005883668114049023 0.0 2 0.0 0.0 0.0 0.008237135359668631 0.0 3 0.0 0.0 0.0 0.009413868982478436 0.0 4 0.0 0.0 0.0 0.03883220955272355 0.0 5 0.0 0.0 0.0 0.04648097810098728 0.0 6 0.0 0.0 0.0 0.04765771172379708 0.0 7 0.0 0.0 0.0 0.0505995457808216 0.0 8 0.0 0.0 0.0 0.0505995457808216 0.0 9 0.0 0.0 0.0 0.055306480272060816 0.0 10 0.0 0.0 0.0 0.06177851519751474 0.0 11 0.0 0.0 0.0 0.06472034925453925 0.0 12 0.0 0.0 0.0 0.06648544968875396 0.0 13 0.0 0.0 0.0 0.06766218331156376 0.0 14 0.0 0.0 0.0 0.06942728374577847 0.0 15 0.0 0.0 0.0 0.07236911780280297 0.0 16 0.0 0.0 0.0 0.07295748461420788 0.0 17 0.0 0.0 0.0 0.07354585142561279 0.0 18 0.0 0.0 0.0 0.08060625316247161 0.0 19 0.0 0.0 0.0 0.08766665489933044 0.0 20 0.0 0.0 0.0 0.09119685576775985 0.0 21 0.0 0.0 0.0 0.09355032301337945 0.0 22 0.0 0.0 0.0 0.09943399112742848 0.0 23 0.0 0.0 0.0 0.1035525588072628 0.0 24 0.0 0.0 0.0 0.10767112648709712 0.0 25 0.0 0.0 0.0 0.11473152822395594 0.0 26 0.0 0.0 0.0 0.1317941657546981 0.0 27 0.0 0.0 0.0 0.21298878572857463 0.0 28 0.0 0.0 0.0 0.28065096904013836 0.0 29 0.0 0.0 0.0 0.37655475929913745 0.0 30 0.0 0.0 0.0 0.4848142525976395 0.0 31 0.0 0.0 0.0 0.6048410821242395 0.0 32 0.0 0.0 0.0 0.746637483672821 0.0 33 0.0 0.0 0.0 0.9502124004189172 0.0 34 0.0 0.0 0.0 1.2414539720643438 0.0 35 0.0 0.0 0.0 1.6303644344029842 0.0 36 0.0 0.0 0.0 2.151069062496323 0.0 37 0.0 0.0 0.0 2.9588966945552535 0.0 38 0.0 0.0 0.0 3.670820536355185 0.0 39 0.0 0.0 0.0 4.412751085536767 0.0 40 0.0 0.0 0.0 5.274708464244949 0.0 41 0.0 0.0 0.0 6.123721773102223 0.0 42 0.0 0.0 0.0 7.0274531954201525 0.0 43 0.0 0.0 0.0 7.958249491062708 0.0 44 0.0 0.0 0.0 8.869629681928902 0.0 45 0.0 0.0 0.0 9.859851025523353 0.0 46 0.0 0.0 0.0 10.852425836363423 0.0 47 0.0 0.0 0.0 11.745566656076065 0.0 48 0.0 0.0 0.0 12.516327179016486 0.0 49 0.0 0.0 0.0 13.230016121250632 0.0 50 0.0 0.0 0.0 13.93664466174792 0.0 51 0.0 0.0 0.0 14.66151257339876 0.0 52 0.0 0.0 0.0 15.436391664019016 0.0 53 0.0 0.0 0.0 16.233040326661254 0.0 54 0.0 0.0 0.0 17.00556595003589 0.0 55 0.0 0.0 0.0 17.703368988362104 0.0 56 0.0 0.0 0.0 18.435297301749802 0.0 57 0.0 0.0 0.0 19.11427260211106 0.0 58 0.0 0.0 0.0 19.797954836963555 0.0 59 0.0 0.0 0.0 20.466339534719527 0.0 60 0.0 0.0 0.0 21.167084407102763 0.0 61 0.0 0.0 0.0 21.83017380355609 0.0 62 0.0 0.0 0.0 22.469140160741812 0.0 63 0.0 0.0 0.0 23.08574857909415 0.0 64 0.0 0.0 0.0 23.744719407867642 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAAGTT 15 0.0022216348 70.0 9 CAAGTTG 15 0.0022216348 70.0 10 ATCGTAT 15 0.0022216348 70.0 39 ATATCGT 15 0.0022216348 70.0 37 AGTTGCG 15 0.0022216348 70.0 12 CGTACCG 15 0.0022216348 70.0 66 CATCGTT 20 7.9092235E-5 70.0 32 TCGTTGT 20 0.0069418997 52.5 34 TGCCCAT 20 0.0069418997 52.5 12 CAGTTTC 20 0.0069418997 52.5 4 CACGCTA 20 0.0069418997 52.5 23 AAGCGCA 20 0.0069418997 52.5 5 CTGGTCG 20 0.0069418997 52.5 44 CGGATGG 20 0.0069418997 52.5 29 GGTATTG 20 0.0069418997 52.5 33 TTGCGGG 20 0.0069418997 52.5 14 GAACGGA 20 0.0069418997 52.5 26 AACTACA 20 0.0069418997 52.5 46 ATGCCCA 20 0.0069418997 52.5 11 CTTTATA 95 0.0 44.21053 2 >>END_MODULE