FastQCFastQC Report
Wed 25 May 2016
SRR1780221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643683
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAG609.779053E-625.65469628
TATTAAG551.5774264E-424.03364215
GCTATAC752.0813695E-623.4411453
ATGCCAT500.00259314421.9794943
TCAGATG602.8919597E-421.9760744
TTCGGTA654.944241E-420.29822226
TAAACCG550.00450968219.9782497
CTTGTAC901.0102131E-519.5342883
ATTAGAG809.0555994E-519.2290653
TATACCG809.0555994E-519.2290656
GGTAAGC708.143179E-418.84834929
CACTTAA708.17729E-418.8366361
CGTTAAA600.007345518.36046217
GACAAAT600.007371940718.3490336
TAAGCTG600.00744839418.3162442
TATGCAG902.2212439E-417.1031330
TCGGTAA902.2212439E-417.1031327
TTGTACT1053.805253E-516.7436774
CTGCCGA4250.016.58921618
AAGTTGT800.001967442216.52441619
Produced by FastQC (version 0.11.5)