FastQCFastQC Report
Wed 25 May 2016
SRR1780221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643683
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT182762.8392857975121295No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC57790.8978021790229042No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG47180.7329694896400868No Hit
CACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTG37570.5836723977485813RNA PCR Primer, Index 14 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC10060.15628811076259588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG5150.036.73818644
GTTAAGC301.3003913E-436.666993
CGTCTTC5200.035.96185742
GGTTACA250.002330438735.2660681
GTCTTCT5450.034.3122343
CCGTCTT5600.033.3931541
TCTTTAT1350.032.6537631
ATGCCGT25800.032.06229844
TATGCCC300.00574497629.33131437
CTTTATA1550.028.3873462
TATGCCG29550.027.77012643
GCCGTCT6950.027.53767240
TATTGCG407.0313667E-427.4981069
TGCCGTC7700.024.56973639
CTCGCTA450.001397432324.4446613
CAACCGT651.8126491E-523.6906766
CCATAGG500.00255120622.0412921
TTAATAC500.002579006422.0001963
CCTGTAT500.0025801721.99848633
TCTATCG500.0025801721.99848629
Produced by FastQC (version 0.11.5)