FastQCFastQC Report
Wed 25 May 2016
SRR1780220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145658
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACG150.002220994870.0000111
TTTAACG200.006939916452.49999634
TAACGGC200.006939916452.49999636
TACTGTG200.006939916452.49999631
TGACAGG200.006939916452.4999964
ATTTCGC200.006939916452.49999657
ATTAGAG305.8624154E-446.6666683
GACACAT350.001252162240.026
CACTAAG350.001252162240.065
GCCCTAA350.001252162240.01
GTGACAC350.001252162240.024
GAGGCAC350.001252162240.07
ACATGTT459.162379E-538.88888529
AGGCACC501.7031736E-435.0000048
TTTGCAC501.7031736E-435.00000445
GACTACT400.002412527334.99999637
CACGACG552.980728E-431.818184
ATCTCGG450.00429623831.11110942
ACCTTTG450.00429623831.11110942
ACCTTAA450.00429623831.1111091