##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780220_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 145658 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.744545442062915 34.0 32.0 34.0 21.0 34.0 2 30.688379628993946 34.0 32.0 34.0 21.0 34.0 3 30.692443944033283 34.0 32.0 34.0 21.0 34.0 4 30.513064850540307 34.0 32.0 34.0 21.0 34.0 5 30.450486756649138 34.0 32.0 34.0 21.0 34.0 6 33.75416386329621 38.0 34.0 38.0 21.0 38.0 7 34.074530750113276 38.0 34.0 38.0 21.0 38.0 8 34.05000755193673 38.0 35.0 38.0 21.0 38.0 9 34.29121641104505 38.0 36.0 38.0 21.0 38.0 10-11 34.087307253978494 38.0 35.0 38.0 21.5 38.0 12-13 34.13552980268849 38.0 35.0 38.0 22.0 38.0 14-15 34.08153688777822 38.0 35.0 38.0 21.0 38.0 16-17 34.1719129742273 38.0 35.5 38.0 21.5 38.0 18-19 34.167659860769746 38.0 35.5 38.0 21.5 38.0 20-21 34.17709634898186 38.0 35.0 38.0 21.5 38.0 22-23 34.18578107621964 38.0 35.5 38.0 21.5 38.0 24-25 34.242468659462574 38.0 36.0 38.0 22.0 38.0 26-27 34.2403815787667 38.0 36.0 38.0 22.0 38.0 28-29 34.33663444506996 38.0 36.0 38.0 22.5 38.0 30-31 34.3612331626138 38.0 36.0 38.0 22.5 38.0 32-33 34.403980557195624 38.0 36.0 38.0 23.0 38.0 34-35 34.379793763473344 38.0 36.0 38.0 23.0 38.0 36-37 34.30867168298343 38.0 36.0 38.0 22.5 38.0 38-39 34.32273544879101 38.0 36.0 38.0 22.5 38.0 40-41 34.37041563113595 38.0 36.0 38.0 23.0 38.0 42-43 34.45477762979033 38.0 36.0 38.0 23.0 38.0 44-45 34.49436694174024 38.0 36.0 38.0 23.0 38.0 46-47 34.415964107704355 38.0 36.0 38.0 23.0 38.0 48-49 34.37857172280273 38.0 36.0 38.0 23.0 38.0 50-51 34.32580428126158 38.0 36.0 38.0 23.0 38.0 52-53 34.37559214049348 38.0 36.0 38.0 23.0 38.0 54-55 34.369337763802875 38.0 36.0 38.0 23.0 38.0 56-57 34.30986626206594 38.0 36.0 38.0 23.0 38.0 58-59 34.36815348281591 38.0 36.0 38.0 23.0 38.0 60-61 34.397461862719524 38.0 36.0 38.0 23.0 38.0 62-63 34.2620041466998 38.0 36.0 38.0 23.0 38.0 64-65 34.26558445124881 38.0 36.0 38.0 23.0 38.0 66-67 34.17076645292397 38.0 35.5 38.0 23.0 38.0 68-69 34.134554916310805 38.0 35.0 38.0 22.5 38.0 70-71 34.12769981738044 38.0 35.5 38.0 22.5 38.0 72-73 34.198811599774814 38.0 36.0 38.0 23.0 38.0 74-75 34.2281920663472 38.0 36.0 38.0 23.0 38.0 76 32.78015625643631 37.0 32.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.6038475215258892 1101 2 -0.43233792198781984 1101 3 -0.2854807914914872 1101 4 0.16780141357881817 1101 5 -0.6510790138202225 1101 6 -0.5206414373154331 1101 7 -0.649683037178427 1101 8 -1.001772906458008 1101 9 -1.114653313696877 1101 10-11 -0.9879556766760302 1101 12-13 -0.45491615162683274 1101 14-15 -0.9579251567786997 1101 16-17 -0.746975460512644 1101 18-19 -0.6491419337861757 1101 20-21 -0.6341922480638473 1101 22-23 -0.8787407796564111 1101 24-25 -0.9505573574927482 1101 26-27 -1.2746004915334055 1101 28-29 -1.0398081825209857 1101 30-31 -0.990728305822806 1101 32-33 -0.6242811105272708 1101 34-35 -0.7939218795058594 1101 36-37 -0.9773794984446269 1101 38-39 -1.0390620704025508 1101 40-41 -0.5981703270986785 1101 42-43 -0.6439841802842139 1101 44-45 -0.8685250742552171 1101 46-47 -0.7289861100279253 1101 48-49 -0.965680417294557 1101 50-51 -0.911677581256022 1101 52-53 -0.7940184943149049 1101 54-55 -0.702059408392465 1101 56-57 -0.504213122149892 1101 58-59 -0.9028731588351846 1101 60-61 -0.6159143420466435 1101 62-63 -0.8533248034182463 1101 64-65 -0.5839102461829455 1101 66-67 -0.5830775661174954 1101 68-69 -1.2321430694907747 1101 70-71 -0.9548363555463837 1101 72-73 -0.8410651813908601 1101 74-75 -1.2981964457160373 1101 76 -1.807607893541924 1102 1 -0.14441665588825714 1102 2 -0.5213567213259118 1102 3 -0.10877243894286082 1102 4 -0.2744306348165928 1102 5 -0.32280947525852 1102 6 -0.6063913762786228 1102 7 -0.27630051269588307 1102 8 -0.5284663388970401 1102 9 -0.6091057456747393 1102 10-11 -0.6601473051381674 1102 12-13 -0.32759336183761434 1102 14-15 -0.44425291089530106 1102 16-17 -0.4730083390087003 1102 18-19 -0.5180212979182315 1102 20-21 -0.34915711798762317 1102 22-23 -0.32408570546277105 1102 24-25 -0.4616796210090115 1102 26-27 -0.6955171288119786 1102 28-29 -0.6725022117644031 1102 30-31 -0.3432837136843503 1102 32-33 -0.6417172931494122 1102 34-35 -0.595158892228639 1102 36-37 -0.6600965860392307 1102 38-39 -0.6424787754998036 1102 40-41 -0.37872264242841425 1102 42-43 -0.18758480964156377 1102 44-45 -0.40250901479179646 1102 46-47 -0.9434287761158728 1102 48-49 -0.7735636606551068 1102 50-51 -0.6091587954816688 1102 52-53 -0.08161717049426898 1102 54-55 -1.0668425242539001 1102 56-57 -0.39002681067226064 1102 58-59 -0.6900310135947052 1102 60-61 -0.8635542519834374 1102 62-63 -0.8419990838620492 1102 64-65 -0.021300040827988198 1102 66-67 -0.45387619889290676 1102 68-69 -0.7230960575818202 1102 70-71 -0.9334463438815703 1102 72-73 -0.4498666896783021 1102 74-75 -0.7355862403610729 1102 76 -0.8125722209162554 1103 1 -0.16097677970226698 1103 2 0.2091661237047049 1103 3 0.09284925542494804 1103 4 0.30799315900616264 1103 5 0.103554834007344 1103 6 -0.5617443134968454 1103 7 -0.3624252687688241 1103 8 -0.7997670589024253 1103 9 -0.32931299956074866 1103 10-11 -0.7473049661300237 1103 12-13 -0.46696823566843904 1103 14-15 -0.45543654591313043 1103 16-17 -0.2516807029142285 1103 18-19 -0.37197459850365533 1103 20-21 -0.6662892086948418 1103 22-23 -0.2853702758146994 1103 24-25 -0.5232064361627664 1103 26-27 -0.8303936512532673 1103 28-29 -0.8642625239910444 1103 30-31 -0.6600524371888241 1103 32-33 -0.2630415647327524 1103 34-35 -0.7139429579038961 1103 36-37 -0.7855524772982321 1103 38-39 -0.8807870993003561 1103 40-41 -0.7435466784959743 1103 42-43 -0.6296916665152565 1103 44-45 -0.4879104009703852 1103 46-47 -0.7071189719304201 1103 48-49 -0.526413401587746 1103 50-51 -0.9423160527321883 1103 52-53 -0.7183946417276985 1103 54-55 -0.671162556553206 1103 56-57 -0.3316626359868451 1103 58-59 -0.7181515976738382 1103 60-61 -0.3998539626354827 1103 62-63 -0.5837694896220853 1103 64-65 -0.4569604943640897 1103 66-67 -0.40378199258108793 1103 68-69 -0.5597557709254701 1103 70-71 -0.8861547753464833 1103 72-73 -0.456969172687522 1103 74-75 -0.445922018572503 1103 76 -0.46978236828184095 1104 1 -0.10871935970291347 1104 2 -0.37562972560727204 1104 3 0.08077101108863971 1104 4 0.3286370312629394 1104 5 -0.5921752423678939 1104 6 0.8074671202307186 1104 7 0.4177107242703926 1104 8 0.02652922545837555 1104 9 0.32577384907448703 1104 10-11 0.5977854273474605 1104 12-13 0.4409142044720653 1104 14-15 0.3812976875952927 1104 16-17 0.3543133030797776 1104 18-19 0.1894832984381125 1104 20-21 -0.3537984736234563 1104 22-23 0.4156351703520329 1104 24-25 0.5636131958181494 1104 26-27 0.3150705619528793 1104 28-29 0.00633462079964886 1104 30-31 0.5109378628337353 1104 32-33 0.421725281324413 1104 34-35 0.41214063592163086 1104 36-37 0.3265257670380777 1104 38-39 0.21664772394091614 1104 40-41 0.06488360090204992 1104 42-43 0.05581315124761943 1104 44-45 0.2512182123517732 1104 46-47 0.3068251484653146 1104 48-49 0.33785458848669947 1104 50-51 0.27052916656464987 1104 52-53 -0.09971010046250939 1104 54-55 0.4909108539073088 1104 56-57 0.5628428585186498 1104 58-59 0.16492983702050168 1104 60-61 0.24831077585184147 1104 62-63 0.16392475162054154 1104 64-65 0.21914082367270993 1104 66-67 0.43603303110490543 1104 68-69 0.5621094606171795 1104 70-71 0.4188531199839929 1104 72-73 0.3056810802853107 1104 74-75 0.32681492190637584 1104 76 -0.4467151129565252 1105 1 0.0670519080279135 1105 2 -0.4020052919443202 1105 3 -0.021681984479428706 1105 4 0.24614008670533138 1105 5 -0.3611282441085706 1105 6 0.4308576119409011 1105 7 -0.028717185807010992 1105 8 -0.16226625125236183 1105 9 -0.02085528811796422 1105 10-11 -0.008676678994277154 1105 12-13 -1.282694797663659 1105 14-15 -0.3039763146021528 1105 16-17 -0.18290445428722535 1105 18-19 -0.23853788596246517 1105 20-21 -0.05305343948892016 1105 22-23 -0.23325746325411956 1105 24-25 -0.07360703059320883 1105 26-27 -0.1387027550847577 1105 28-29 -0.2141849895851493 1105 30-31 -0.33011123411329635 1105 32-33 -0.23038019625048634 1105 34-35 -0.2401592788963427 1105 36-37 -0.31604611301574437 1105 38-39 -0.09402286850627917 1105 40-41 -0.3505732340299943 1105 42-43 -0.16052451525855815 1105 44-45 -0.28292558479154195 1105 46-47 -0.42394716860936654 1105 48-49 -0.4326323070603806 1105 50-51 -0.6931172415930789 1105 52-53 -0.574032039156144 1105 54-55 -0.5541276014585463 1105 56-57 -0.490831079483641 1105 58-59 -0.4382330088000188 1105 60-61 -0.3807029643799993 1105 62-63 -0.7728306771907398 1105 64-65 -0.724861250324544 1105 66-67 -0.5685038378999465 1105 68-69 -0.3280118929129401 1105 70-71 -0.34592411048895144 1105 72-73 -0.4678982933181359 1105 74-75 -0.5374061563252894 1105 76 -0.16228737571222496 1106 1 -0.4654270851808491 1106 2 -0.07085257225229213 1106 3 -0.0570322255619331 1106 4 -0.11170436711809728 1106 5 0.1464687324482341 1106 6 -0.24795270664611024 1106 7 -0.2545162112453738 1106 8 -0.13424440674738491 1106 9 -0.036253873596741926 1106 10-11 -0.7291264865119658 1106 12-13 -0.304513723599527 1106 14-15 -0.42647116681921204 1106 16-17 0.00993916821721541 1106 18-19 -0.20615481417487302 1106 20-21 -0.393405023471324 1106 22-23 0.10980549956673258 1106 24-25 -0.2597169693314001 1106 26-27 -0.07782462644773602 1106 28-29 -0.2869413875439406 1106 30-31 -0.2986362184461271 1106 32-33 -0.2095076950035093 1106 34-35 -0.43013165352704164 1106 36-37 -0.23747048217253308 1106 38-39 -0.4940311816961085 1106 40-41 -0.4034738978062222 1106 42-43 -0.08569941198315689 1106 44-45 -0.18492906354175176 1106 46-47 -0.008821639442857077 1106 48-49 -0.2631743270376177 1106 50-51 -0.6495273504319172 1106 52-53 -0.27704652968549937 1106 54-55 -0.5167406288665006 1106 56-57 -0.5530361211135357 1106 58-59 -0.453580436187508 1106 60-61 -0.8277543398931826 1106 62-63 -0.7912411952943117 1106 64-65 -0.5015369975524138 1106 66-67 -0.6013281482249297 1106 68-69 -0.335327499967633 1106 70-71 -0.23581591112586864 1106 72-73 -0.14343129668178278 1106 74-75 -0.6869028886022122 1106 76 0.16706202126177772 1107 1 -0.22197095793782395 1107 2 -0.6741298906257924 1107 3 -0.7257356079875024 1107 4 0.03428066460401524 1107 5 -0.4459749465569196 1107 6 -1.0787617787901738 1107 7 -0.13551080392332437 1107 8 -0.400349235346674 1107 9 0.1312781334441766 1107 10-11 -0.21035915332215183 1107 12-13 0.10390055071758297 1107 14-15 -0.5104746112960186 1107 16-17 -0.01666055065268779 1107 18-19 0.01754622549992746 1107 20-21 0.09521997177986208 1107 22-23 0.17441476672896528 1107 24-25 -0.023855786812120527 1107 26-27 0.2762789864538533 1107 28-29 0.25743474425794943 1107 30-31 -0.16318723342706676 1107 32-33 -0.13261164982007756 1107 34-35 0.34729752575037764 1107 36-37 0.1096333713358959 1107 38-39 0.029871207909671682 1107 40-41 0.2694179430893371 1107 42-43 0.6454762689974984 1107 44-45 0.19623648662477677 1107 46-47 0.10685326273146956 1107 48-49 0.34575408609698144 1107 50-51 -0.08129948309493074 1107 52-53 -0.20677350424449514 1107 54-55 0.19119749718615964 1107 56-57 -0.05112076406565791 1107 58-59 0.05018696031989833 1107 60-61 0.14015947220554636 1107 62-63 0.01423767562645395 1107 64-65 0.19565929963501816 1107 66-67 0.23399230208976007 1107 68-69 0.34532707726082634 1107 70-71 0.28663967158590253 1107 72-73 0.11094394867387791 1107 74-75 0.14393467645660962 1107 76 0.5852556971010472 1108 1 -0.7102448144664848 1108 2 -0.29682508610329705 1108 3 -0.9366871115160009 1108 4 -0.49232408100573366 1108 5 -0.24914996755241958 1108 6 0.18922227716440432 1108 7 -0.20223889026183883 1108 8 0.04190373849356632 1108 9 -0.21735371202894527 1108 10-11 -0.29655978256041493 1108 12-13 -0.5461295323869919 1108 14-15 -0.28231933233789874 1108 16-17 -0.218499380419928 1108 18-19 -0.5666112595525377 1108 20-21 -0.5141164450716929 1108 22-23 -0.4992462217517186 1108 24-25 -0.6580797053871805 1108 26-27 -0.2655114596535242 1108 28-29 -0.10050719535021813 1108 30-31 -0.18503508812567304 1108 32-33 -0.5171144638438179 1108 34-35 0.037469751524078276 1108 36-37 -0.5822247617567697 1108 38-39 -0.2538250028726097 1108 40-41 -0.3298657029829357 1108 42-43 0.01846602820731391 1108 44-45 -0.42373869481394877 1108 46-47 -0.3870799778456231 1108 48-49 -0.18977218021370135 1108 50-51 -0.34997607718610624 1108 52-53 -0.49862662348640185 1108 54-55 -0.62118734092914 1108 56-57 -0.4480203241002556 1108 58-59 -0.5620794255711274 1108 60-61 -0.08663893338991357 1108 62-63 0.06403461467056104 1108 64-65 -0.21441728693538664 1108 66-67 -0.2159726009549061 1108 68-69 -0.19816494444914667 1108 70-71 0.05141172939559624 1108 72-73 -0.49912574385173514 1108 74-75 -0.23213860097229144 1108 76 0.18534469295208567 1109 1 -0.36675893724321895 1109 2 -0.08672240987226942 1109 3 0.20154859628509314 1109 4 -0.15331989979433303 1109 5 -0.24191276372156167 1109 6 -0.589480396509309 1109 7 0.2736903743819923 1109 8 -0.31638260416118413 1109 9 0.13551112104226348 1109 10-11 0.48600443803365323 1109 12-13 -0.08409608269578683 1109 14-15 0.07124556594486364 1109 16-17 0.20781156361321962 1109 18-19 0.18364766867639304 1109 20-21 0.14493901032232515 1109 22-23 0.2239914679691566 1109 24-25 -0.44847890239000776 1109 26-27 0.09124049349645702 1109 28-29 -0.09478200451052032 1109 30-31 -0.13104375742637586 1109 32-33 -0.1441842913528717 1109 34-35 0.28243503596153374 1109 36-37 -0.3269151347412915 1109 38-39 -0.1387489866592304 1109 40-41 -0.5759913694833756 1109 42-43 0.04365558924405377 1109 44-45 0.04109501572347085 1109 46-47 -0.18526620158518625 1109 48-49 -0.11359933123598154 1109 50-51 0.13251589923544316 1109 52-53 -0.074300795875061 1109 54-55 0.11772669489886312 1109 56-57 -0.08133959219645703 1109 58-59 -0.2279277511685791 1109 60-61 -0.28515057557027745 1109 62-63 -0.05813155971280537 1109 64-65 -0.10986462540892461 1109 66-67 0.0814624112562754 1109 68-69 -0.01488531474848287 1109 70-71 0.08794298618846597 1109 72-73 -0.08601374620445768 1109 74-75 0.22861299415346537 1109 76 -0.9675700077780256 1110 1 0.026292740900586864 1110 2 0.15327386781245167 1110 3 0.2545295504385763 1110 4 0.010927261940267385 1110 5 0.0750796055321139 1110 6 0.2316499465640831 1110 7 0.20024909390553347 1110 8 0.49268123354586635 1110 9 0.4830326794515969 1110 10-11 0.34246575697402903 1110 12-13 0.1230910877242195 1110 14-15 0.2898416326983835 1110 16-17 0.4998023819021995 1110 18-19 0.2848636250012362 1110 20-21 0.19854965614403142 1110 22-23 0.16923931212822652 1110 24-25 0.25330470701504026 1110 26-27 0.1467588258992123 1110 28-29 0.6576871312919508 1110 30-31 0.3929217491186918 1110 32-33 0.3014605997694133 1110 34-35 0.012015034720761264 1110 36-37 0.22560673386097818 1110 38-39 0.35313946795954365 1110 40-41 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0.22481568176999644 2116 8 0.6563534163669829 2116 9 0.02706997916265408 2116 10-11 0.6947543043866844 2116 12-13 0.8423713609051973 2116 14-15 0.6101167837359469 2116 16-17 0.5232985731288835 2116 18-19 0.7367121934278131 2116 20-21 0.7373892936538766 2116 22-23 0.9220696247103035 2116 24-25 0.6569952770176641 2116 26-27 0.6428482664035471 2116 28-29 0.7190477827235213 2116 30-31 0.5421961121341923 2116 32-33 0.6386059898403289 2116 34-35 0.6593127757241746 2116 36-37 0.6390917614417546 2116 38-39 0.8615450366487991 2116 40-41 0.6037972010287547 2116 42-43 0.5421721080080886 2116 44-45 0.6179821107731911 2116 46-47 0.5134668252511219 2116 48-49 0.4645440452207694 2116 50-51 0.8385660970329454 2116 52-53 0.7705284033123618 2116 54-55 0.4620614003409642 2116 56-57 0.339234020548119 2116 58-59 0.3651172611269402 2116 60-61 0.352525838414536 2116 62-63 0.6837560699247405 2116 64-65 0.21861286844331573 2116 66-67 0.31038628594889417 2116 68-69 0.7632340052557964 2116 70-71 0.45637423819317746 2116 72-73 0.11570629014751432 2116 74-75 0.6798585838038491 2116 76 0.787802599941422 2117 1 0.5792973108480659 2117 2 -0.1290056492736973 2117 3 0.3718476051415287 2117 4 0.20787835668141597 2117 5 0.06560549053313736 2117 6 0.7210640383722833 2117 7 0.7215095809118779 2117 8 0.3007639018495283 2117 9 0.22317318828455512 2117 10-11 0.22980632037351967 2117 12-13 -0.165504506027915 2117 14-15 0.5713913223512037 2117 16-17 -0.3719935392491607 2117 18-19 0.006761858409596755 2117 20-21 -0.004683411177431651 2117 22-23 0.021855095499070387 2117 24-25 0.09078509716577798 2117 26-27 0.12835285086340065 2117 28-29 0.08339479106959402 2117 30-31 -0.04892737440629702 2117 32-33 -0.013364446271104669 2117 34-35 -3.393874626311799E-5 2117 36-37 -0.24217513640707722 2117 38-39 -0.13548681946969054 2117 40-41 -0.10102676746777206 2117 42-43 0.062229119880662154 2117 44-45 -0.07684273935024066 2117 46-47 0.12425793845201127 2117 48-49 0.2849125652630846 2117 50-51 -0.17510646673806463 2117 52-53 0.03212914387895438 2117 54-55 0.050789800482021974 2117 56-57 -0.4154826833428018 2117 58-59 0.1778303209600196 2117 60-61 0.17675147612465736 2117 62-63 0.20922039342303833 2117 64-65 0.26046075841627925 2117 66-67 -0.04815667866848372 2117 68-69 -0.07733846447501236 2117 70-71 0.04990750491469953 2117 72-73 -1.1836427753279821 2117 74-75 -0.4980243361444323 2117 76 -0.504897529363852 2118 1 0.4528053565288488 2118 2 0.37312053663871225 2118 3 0.4680414102089401 2118 4 0.04051369968997065 2118 5 0.07212096175714322 2118 6 0.6243145256232197 2118 7 0.3802728494394927 2118 8 0.14680065909386286 2118 9 -0.08733049227211609 2118 10-11 -0.3333107970338176 2118 12-13 0.25593131376172096 2118 14-15 0.04512004035774453 2118 16-17 0.09719287248996267 2118 18-19 -0.18040290489114597 2118 20-21 0.2627327897149172 2118 22-23 0.09554676632683368 2118 24-25 0.030808936219955285 2118 26-27 -0.02607348162698031 2118 28-29 -0.3272892931402538 2118 30-31 4.299375792626847E-4 2118 32-33 0.3173984034623203 2118 34-35 -0.02510118742947043 2118 36-37 0.22351192768454808 2118 38-39 0.07269819091963114 2118 40-41 -0.11388858149094006 2118 42-43 0.22180581559446466 2118 44-45 0.18985265230286785 2118 46-47 0.08750069411781425 2118 48-49 -0.19026179808920318 2118 50-51 0.2890383071936 2118 52-53 0.11269467711672121 2118 54-55 -0.179140293102364 2118 56-57 0.38174844273036257 2118 58-59 0.20199152451010605 2118 60-61 -0.019360986224135956 2118 62-63 -0.012579455166751075 2118 64-65 0.06511742771383666 2118 66-67 0.0018368783439015601 2118 68-69 0.039581626371109735 2118 70-71 0.12023271471447572 2118 72-73 -0.0550608769016776 2118 74-75 -0.17030579805832247 2118 76 -0.08987248224951117 2119 1 1.27003698265478 2119 2 1.0034673448715985 2119 3 0.5026961249733084 2119 4 0.6028565046157794 2119 5 0.9537971290257587 2119 6 0.628370049751851 2119 7 1.0966559485213452 2119 8 1.3402213109366912 2119 9 1.0438213287974634 2119 10-11 0.9059999379211447 2119 12-13 0.9675539585552428 2119 14-15 1.024133138134168 2119 16-17 0.8188368392451082 2119 18-19 0.8211636065748493 2119 20-21 0.3914739236951661 2119 22-23 0.5167425367458733 2119 24-25 0.547039735575936 2119 26-27 0.5916455036217059 2119 28-29 0.883576204444708 2119 30-31 0.50371961165785 2119 32-33 0.6651421918092097 2119 34-35 0.338198930058887 2119 36-37 0.8031081158399758 2119 38-39 0.5420467832350226 2119 40-41 0.5904715420894178 2119 42-43 0.4325103106120878 2119 44-45 0.7829948132184086 2119 46-47 0.6713264632314306 2119 48-49 0.5810492849794215 2119 50-51 0.584743454924336 2119 52-53 0.3180077618388779 2119 54-55 0.5098027468001618 2119 56-57 0.45170623394919573 2119 58-59 0.41699087179699745 2119 60-61 0.47452091621700987 2119 62-63 0.4246817606202029 2119 64-65 0.36916860042172317 2119 66-67 0.5329886994134867 2119 68-69 0.20731149017164086 2119 70-71 0.5816878298095602 2119 72-73 0.6129474778261397 2119 74-75 0.9705540426796802 2119 76 1.2760210819992182 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 4.0 13 139.0 14 750.0 15 1663.0 16 2235.0 17 2440.0 18 2445.0 19 2075.0 20 1647.0 21 1240.0 22 981.0 23 866.0 24 814.0 25 905.0 26 996.0 27 1171.0 28 1387.0 29 1766.0 30 2186.0 31 2785.0 32 3551.0 33 4727.0 34 6774.0 35 10372.0 36 20470.0 37 71269.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.86931030221478 18.630627909211988 10.547995990608138 28.952065797965098 2 21.363055925524176 20.063436268519407 30.211866152219585 28.361641653736836 3 20.403273421301954 21.48251383377501 28.262093396861143 29.8521193480619 4 15.814442049183706 18.147303958587926 32.67173790660314 33.36651608562523 5 14.851913386151121 31.169589037334028 32.45891059879993 21.519586977714923 6 28.135770091584394 34.951049719205265 18.375235139848137 18.537945049362204 7 28.10007002704966 29.651649754905325 20.2474289088138 22.000851309231212 8 24.82527564569059 31.523156984168395 20.980653311180987 22.67091405896003 9 24.34744401268725 18.380040917766273 20.7582144475415 36.51430062200497 10-11 25.630586716829836 23.78104875804968 25.534471158467092 25.053893366653394 12-13 25.126323305276742 21.53709374013099 25.233766768732234 28.10281618586003 14-15 24.02477035246948 22.408655892570266 23.31179887132873 30.25477488363152 16-17 24.799873676694723 24.93718161721292 23.861373903252826 26.40157080283953 18-19 23.563072402477037 24.07282813165085 25.337090993972183 27.027008471899933 20-21 23.546938719466144 24.34778728253855 25.46238448969504 26.642889508300264 22-23 25.125293495722858 24.190912960496505 24.47582693707177 26.207966606708865 24-25 24.052918480275714 25.243721594419803 24.29492372543904 26.408436199865438 26-27 23.071166705570583 25.674181987944362 25.355970835793435 25.89868047069162 28-29 23.921446127229537 24.574688654244873 26.54471433082975 24.959150887695834 30-31 23.792719932993727 24.34744401268725 26.13416358868033 25.725672465638688 32-33 22.9324856856472 25.09096651059331 26.28108308503481 25.695464718724686 34-35 23.224265059248374 24.85205069409164 26.396765024921393 25.52691922173859 36-37 23.485150146232954 24.12500514904777 26.25259168737728 26.137253017341994 38-39 22.811311428139888 24.136676323991814 27.803141605679055 25.248870642189235 40-41 22.408999162421566 23.392810556234465 28.4481456562633 25.75004462508067 42-43 21.49899078663719 23.461807796344864 28.18966345823779 26.849537958780157 44-45 20.767139463675186 22.9709319089923 29.674992104793418 26.5869365225391 46-47 20.667934476650785 23.81468920347664 29.717214296502764 25.80016202336981 48-49 20.15165662030235 24.22695629488253 29.683917120927106 25.93746996388801 50-51 19.881846517184087 25.33949388293125 28.45913029150476 26.319529308379902 52-53 18.995523761139108 25.082728034162216 28.855950239602357 27.065797965096323 54-55 18.894945694709524 25.059042414422827 28.560738167488225 27.48527372337942 56-57 19.54063628499636 25.123233876615085 28.14057586950253 27.19555396888602 58-59 19.875667659860767 24.69963888011644 27.90200332285216 27.52269013717063 60-61 19.58285847670571 24.293893915885157 28.041714152329426 28.081533455079704 62-63 20.063779538370703 24.116766672616677 27.533674772412088 28.285779016600532 64-65 20.411855167584342 24.71920526164028 26.369646706669048 28.49929286410633 66-67 20.54779002869736 25.27701876999547 25.32267366021777 28.8525175410894 68-69 20.32123192684233 26.07477790440621 24.967046094275634 28.636944074475828 70-71 20.611294951187027 26.66760493759354 24.79300827966881 27.928091831550617 72-73 20.47536009007401 26.949772755358442 24.680759038295186 27.894108116272363 74-75 20.35693199137706 28.21849812574661 24.615881036400335 26.80868884647599 76 20.960057120103258 28.481099561987673 23.977399112990703 26.58144420491837 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.5 10 3.0 11 3.5 12 2.0 13 1.5 14 3.0 15 7.5 16 10.5 17 11.0 18 12.0 19 23.0 20 38.5 21 44.0 22 54.0 23 99.0 24 143.0 25 152.0 26 238.0 27 384.5 28 553.5 29 662.0 30 820.0 31 1055.5 32 1387.5 33 1642.0 34 1936.5 35 2566.0 36 3190.5 37 3480.0 38 3993.0 39 5112.5 40 6360.5 41 7303.5 42 7605.0 43 7802.5 44 8342.0 45 8780.0 46 8876.0 47 9011.5 48 8768.5 49 8121.5 50 7853.0 51 7380.5 52 6234.0 53 5188.0 54 4816.0 55 4645.0 56 4195.5 57 3807.0 58 3697.0 59 3482.5 60 3230.0 61 3097.0 62 3002.0 63 2987.5 64 2804.5 65 2535.5 66 2394.0 67 2353.0 68 2145.0 69 1749.5 70 1365.0 71 1168.0 72 962.5 73 607.5 74 340.5 75 223.0 76 150.5 77 56.0 78 29.0 79 24.0 80 21.5 81 13.5 82 5.5 83 3.0 84 3.5 85 5.5 86 7.0 87 7.0 88 5.0 89 4.0 90 4.0 91 4.0 92 5.0 93 6.5 94 6.0 95 4.0 96 4.0 97 5.0 98 6.5 99 23.0 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 145658.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.12342610773182 #Duplication Level Percentage of deduplicated Percentage of total 1 74.22838430990036 43.88636394842714 2 13.62781300076639 16.114459899215973 3 4.809679741749692 8.530942344395777 4 2.1911795443461295 5.182001675156874 5 1.2691887874776469 3.7519394746598196 6 0.9150235723077638 3.2459597138502523 7 0.6851064818040363 2.8354089717008333 8 0.4772521424092524 2.257342542119211 9 0.3576488074502427 1.9030880555822545 >10 1.4375624143616899 12.256793310357137 >50 0.0011611974267865023 0.03570006453473205 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006178857323319007 0.0 2 0.0 0.0 0.0 0.006865397025910008 0.0 3 0.0 0.0 0.0 0.007551936728501009 0.0 4 0.0 0.0 0.0 0.03501352483214104 0.0 5 0.0 0.0 0.0 0.04325200126323305 0.0 6 0.0 0.0 0.0 0.04325200126323305 0.0 7 0.0 0.0 0.0 0.04325200126323305 0.0 8 0.0 0.0 0.0 0.04325200126323305 0.0 9 0.0 0.0 0.0 0.05080393799173406 0.0 10 0.0 0.0 0.0 0.059042414422826074 0.0 11 0.0 0.0 0.0 0.06041549382800807 0.0 12 0.0 0.0 0.0 0.06041549382800807 0.0 13 0.0 0.0 0.0 0.06316165263837208 0.0 14 0.0 0.0 0.0 0.06384819234096308 0.0 15 0.0 0.0 0.0 0.06384819234096308 0.0 16 0.0 0.0 0.0 0.06522127174614507 0.0 17 0.0 0.0 0.0 0.06796743055650908 0.0 18 0.0 0.0 0.0 0.07002704966428208 0.0 19 0.0 0.0 0.0 0.07414628787982809 0.0 20 0.0 0.0 0.0 0.07551936728501009 0.0 21 0.0 0.0 0.0 0.0810116849057381 0.0 22 0.0 0.0 0.0 0.0871905422290571 0.0 23 0.0 0.0 0.0 0.08925016133683011 0.0 24 0.0 0.0 0.0 0.09130978044460311 0.0 25 0.0 0.0 0.0 0.09748863776792212 0.0 26 0.0 0.0 0.0 0.10847327300937813 0.0 27 0.0 0.0 0.0 0.20252921226434525 0.0 28 0.0 0.0 0.0 0.28628705598044735 0.0 29 0.0 0.0 0.0 0.3789699158302325 0.0 30 0.0 0.0 0.0 0.4840104903266556 0.0 31 0.0 0.0 0.0 0.6130799544137637 0.0 32 0.0 0.0 0.0 0.7387167199879169 0.0 33 0.0 0.0 0.0 0.9130978044460312 0.0 34 0.0 0.0 0.0 1.1657444149995195 0.0 35 0.0 0.0 0.0 1.549520108747889 0.0 36 0.0 0.0 0.0 2.0980653311180983 0.0 37 0.0 0.0 0.0 2.8175589394334675 0.0 38 0.0 0.0 0.0 3.6544508368918973 0.0 39 0.0 0.0 0.0 4.455642669815595 0.0 40 0.0 0.0 0.0 5.430529047494817 0.0 41 0.0 0.0 0.0 6.462398220489091 0.0 42 0.0 0.0 0.0 7.636381111919702 0.0 43 0.0 0.0 0.0 8.741710033091215 0.0 44 0.0 0.0 0.0 9.869008224745636 0.0 45 0.0 0.0 0.0 10.948935176921282 0.0 46 0.0 0.0 0.0 12.016504414450287 0.0 47 0.0 0.0 0.0 13.016792761125377 0.0 48 0.0 0.0 0.0 13.92577132735586 0.0 49 0.0 0.0 0.0 14.82445179804748 0.0 50 0.0 0.0 0.0 15.692237982122506 0.0 51 0.0 0.0 0.0 16.577187658762305 0.0 52 0.0 0.0 0.0 17.50607587636793 0.0 53 0.0 0.0 0.0 18.411621744085462 0.0 54 0.0 0.0 0.0 19.404358154032046 0.0 55 0.0 0.0 0.0 20.278323195430392 0.0 56 0.0 0.0 0.0 21.15434785593651 0.0 57 0.0 0.0 0.0 21.966524324101663 0.0 58 0.0 0.0 0.0 22.715539139628444 0.0 59 0.0 0.0 0.0 23.394526905490945 0.0 60 0.0 6.865397025910008E-4 0.0 24.103035878564857 0.0 61 0.0 6.865397025910008E-4 0.0 24.796440978181767 0.0 62 0.0 6.865397025910008E-4 0.0 25.46924988672095 0.0 63 0.0 6.865397025910008E-4 0.0 26.192862733251864 0.0 64 0.0 6.865397025910008E-4 0.0 26.940504469373465 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTACG 15 0.0022209948 70.00001 11 TTTAACG 20 0.0069399164 52.499996 34 TAACGGC 20 0.0069399164 52.499996 36 TACTGTG 20 0.0069399164 52.499996 31 TGACAGG 20 0.0069399164 52.499996 4 ATTTCGC 20 0.0069399164 52.499996 57 ATTAGAG 30 5.8624154E-4 46.666668 3 GACACAT 35 0.0012521622 40.0 26 CACTAAG 35 0.0012521622 40.0 65 GCCCTAA 35 0.0012521622 40.0 1 GTGACAC 35 0.0012521622 40.0 24 GAGGCAC 35 0.0012521622 40.0 7 ACATGTT 45 9.162379E-5 38.888885 29 AGGCACC 50 1.7031736E-4 35.000004 8 TTTGCAC 50 1.7031736E-4 35.000004 45 GACTACT 40 0.0024125273 34.999996 37 CACGACG 55 2.980728E-4 31.81818 4 ATCTCGG 45 0.004296238 31.111109 42 ACCTTTG 45 0.004296238 31.111109 42 ACCTTAA 45 0.004296238 31.111109 1 >>END_MODULE