FastQCFastQC Report
Wed 25 May 2016
SRR1780220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145658
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT61214.202309519559516No Hit
CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG20671.4190775652555987RNA PCR Primer, Index 18 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC18691.2831427041425805No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG16791.1527001606502905No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4500.30894286616595035No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2580.17712724326847823RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT2560.1757541638632962No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT1660.11396559063010614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCA200.006939916452.49999655
CTTACAG200.006939916452.49999669
TCTTTAT1000.049.0000041
CTTTATA1000.049.0000042
GCTTTAC451.6755966E-646.6666641
TATTAAC404.574975E-543.7499962
ACGTTCT350.001252162240.039
ATCACTC350.001252162240.057
CCACGTT350.001252162240.037
GTATAAG459.162379E-538.8888851
TTTATAC1650.036.0606083
AGAACTC501.7031736E-435.00000417
ATAAGAG501.7031736E-435.0000043
TATAAGA501.7031736E-435.0000042
TATCACT400.002412527334.99999656
AAGAGAC552.980728E-431.818185
TGATCAA450.00429623831.11110948
CTACTGG450.00429623831.1111099
TTGTATT450.00429623831.11110937
GTGCTAG450.00429623831.11110927