##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780220_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 145658 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.68748712738058 34.0 34.0 34.0 34.0 34.0 2 33.67249996567301 34.0 34.0 34.0 34.0 34.0 3 33.70110807507998 34.0 34.0 34.0 34.0 34.0 4 33.743543094097134 34.0 34.0 34.0 34.0 34.0 5 33.745616443998955 34.0 34.0 34.0 34.0 34.0 6 37.48221862170289 38.0 38.0 38.0 37.0 38.0 7 37.50060415493828 38.0 38.0 38.0 37.0 38.0 8 37.39036647489324 38.0 38.0 38.0 37.0 38.0 9 37.39977893421577 38.0 38.0 38.0 37.0 38.0 10-11 37.34622197201664 38.0 38.0 38.0 37.0 38.0 12-13 37.33906136291861 38.0 38.0 38.0 37.0 38.0 14-15 37.25701643576048 38.0 38.0 38.0 36.0 38.0 16-17 37.29098298754617 38.0 38.0 38.0 36.0 38.0 18-19 37.269751747243546 38.0 38.0 38.0 36.0 38.0 20-21 37.281707836164166 38.0 38.0 38.0 36.0 38.0 22-23 37.269089236430545 38.0 38.0 38.0 36.0 38.0 24-25 37.29528759148141 38.0 38.0 38.0 36.0 38.0 26-27 37.1798081808071 38.0 38.0 38.0 36.0 38.0 28-29 37.135169369344624 38.0 38.0 38.0 36.0 38.0 30-31 37.15551497343091 38.0 38.0 38.0 36.0 38.0 32-33 37.044233753037936 38.0 38.0 38.0 36.0 38.0 34-35 37.10491356465144 38.0 38.0 38.0 36.0 38.0 36-37 37.081052877287895 38.0 38.0 38.0 36.0 38.0 38-39 36.50889068914856 38.0 37.5 38.0 34.0 38.0 40-41 36.7961320353156 38.0 37.5 38.0 34.5 38.0 42-43 37.017300800505296 38.0 38.0 38.0 36.0 38.0 44-45 37.09331104367766 38.0 38.0 38.0 36.0 38.0 46-47 37.03787982809046 38.0 38.0 38.0 36.0 38.0 48-49 37.055733293056335 38.0 38.0 38.0 36.0 38.0 50-51 36.92008334591989 38.0 38.0 38.0 35.0 38.0 52-53 36.926471597852505 38.0 38.0 38.0 35.5 38.0 54-55 36.90159483172912 38.0 37.5 38.0 35.5 38.0 56-57 36.94465803457414 38.0 38.0 38.0 36.0 38.0 58-59 36.850200469593155 38.0 37.5 38.0 35.0 38.0 60-61 36.869612379683915 38.0 38.0 38.0 35.0 38.0 62-63 36.83670653173873 38.0 37.5 38.0 35.0 38.0 64-65 36.787279105850686 38.0 37.5 38.0 34.5 38.0 66-67 36.507267022751925 38.0 37.0 38.0 34.0 38.0 68-69 36.44666959590273 38.0 37.0 38.0 34.0 38.0 70-71 35.95456136978402 38.0 37.0 38.0 32.5 38.0 72-73 35.17792362932349 38.0 37.0 38.0 26.0 38.0 74-75 34.92317277458156 38.0 37.0 38.0 24.0 38.0 76 34.24810858311936 38.0 35.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3131818829877986 1101 2 -0.2614212867953043 1101 3 -0.08133755717962998 1101 4 -0.06037574335969964 1101 5 -0.04415523075553551 1101 6 -0.08505102216027183 1101 7 0.03780065783113429 1101 8 0.02612027116844473 1101 9 0.02727818211639743 1101 10-11 0.08301154220509233 1101 12-13 0.16111659162072556 1101 14-15 0.20391378469570043 1101 16-17 0.1646622071738406 1101 18-19 0.0336548248976456 1101 20-21 -0.024977835726787134 1101 22-23 -0.06612177358906024 1101 24-25 0.009345467557828613 1101 26-27 0.09662753864628826 1101 28-29 -0.11546514721280943 1101 30-31 -0.10066865281532955 1101 32-33 -0.3437617476879993 1101 34-35 0.09675819409474684 1101 36-37 0.027565574457327102 1101 38-39 0.02818470846133181 1101 40-41 -0.08447059315803784 1101 42-43 0.007430337761988426 1101 44-45 0.06309882198435446 1101 46-47 -0.2903758884750687 1101 48-49 0.06429256487879798 1101 50-51 -0.03441015881968923 1101 52-53 0.050133219511245386 1101 54-55 0.15812121867138984 1101 56-57 0.15852941598504344 1101 58-59 -0.22944094552537564 1101 60-61 -0.27857223405910503 1101 62-63 -0.15222733228546304 1101 64-65 -0.26208995940296376 1101 66-67 -0.6903863057050046 1101 68-69 -0.6810753698647787 1101 70-71 -0.7364904294551238 1101 72-73 -0.7645355118516335 1101 74-75 -0.5757420533110533 1101 76 -0.8283990456093235 1102 1 0.12858899838376914 1102 2 -0.04795520969902611 1102 3 -0.08771251309749317 1102 4 0.012881994751680281 1102 5 -0.06421599629279484 1102 6 0.06372959797374023 1102 7 -0.06609755989395438 1102 8 -0.10003603732516098 1102 9 -0.2932871543998914 1102 10-11 -0.010937675167333794 1102 12-13 0.11430813870045142 1102 14-15 0.1628563558374907 1102 16-17 0.013351950276685898 1102 18-19 -0.10398996798410565 1102 20-21 -0.10152477981706909 1102 22-23 0.037790007872033016 1102 24-25 -0.08208767678745232 1102 26-27 -0.15363017233762832 1102 28-29 -0.06658144268523358 1102 30-31 0.04116733448901755 1102 32-33 0.16587528673676388 1102 34-35 0.17523797056437473 1102 36-37 -0.005699488071492453 1102 38-39 -0.06532368749125084 1102 40-41 0.018078032835511237 1102 42-43 -0.017452335921646522 1102 44-45 -0.05240995375283575 1102 46-47 0.13560002232980395 1102 48-49 0.06436716542676635 1102 50-51 -0.05538647653664697 1102 52-53 0.2449084684058036 1102 54-55 -0.03688461373509 1102 56-57 -0.012176983385600693 1102 58-59 0.22381163836633533 1102 60-61 0.2477346319040521 1102 62-63 0.18654731980470984 1102 64-65 0.20540454728395474 1102 66-67 0.10567614173551476 1102 68-69 0.05850768089072034 1102 70-71 -0.09388130919904114 1102 72-73 -0.10597543806491672 1102 74-75 -0.19921274207757023 1102 76 -0.16726186198346227 1103 1 -0.006967155307101791 1103 2 0.10688632182644398 1103 3 0.17620646947434437 1103 4 0.07977520516821102 1103 5 0.051799076717095716 1103 6 0.013187911680759612 1103 7 0.15058368632097086 1103 8 -0.0966647971663619 1103 9 0.07324268881389173 1103 10-11 -0.01912689527365785 1103 12-13 -0.19356145284789505 1103 14-15 3.134583205053332E-4 1103 16-17 -0.07021914184362998 1103 18-19 0.03924400012033402 1103 20-21 0.10674855306766062 1103 22-23 0.04123526727583737 1103 24-25 0.10682625901768006 1103 26-27 0.0646155715557839 1103 28-29 0.14142272930857303 1103 30-31 0.08065996620962324 1103 32-33 0.24043285352230725 1103 34-35 -0.04487844152094311 1103 36-37 0.05287665081552717 1103 38-39 0.03449462890738175 1103 40-41 -0.1252164964693847 1103 42-43 0.05527830131676126 1103 44-45 -0.03674478990114949 1103 46-47 -0.09116802827244896 1103 48-49 0.0010369723038294865 1103 50-51 -0.09596248084321957 1103 52-53 0.13100967290079524 1103 54-55 0.07837007975793853 1103 56-57 -0.22665111029495932 1103 58-59 -0.02400815841718895 1103 60-61 -0.0792453828997921 1103 62-63 -0.014694184804667998 1103 64-65 0.3585974683525208 1103 66-67 -0.25899649133090463 1103 68-69 -0.08306421871227343 1103 70-71 -0.33315188318463385 1103 72-73 -0.3602416381860394 1103 74-75 -0.27378040239652535 1103 76 -0.6007387477919579 1104 1 0.1564178467875763 1104 2 0.022918676568473018 1104 3 -0.029027006726877858 1104 4 0.09026149307385367 1104 5 0.05906278075499216 1104 6 0.11036815079648221 1104 7 0.032714458774322 1104 8 -0.17960121558665065 1104 9 -0.13928671726390718 1104 10-11 -0.04171076301881982 1104 12-13 -0.017189437766198523 1104 14-15 -0.1700387700962409 1104 16-17 -0.12691405982887005 1104 18-19 0.010237522726754378 1104 20-21 0.18346538462320439 1104 22-23 0.0029284128399496012 1104 24-25 0.1339378570970169 1104 26-27 0.0847857239839982 1104 28-29 0.011861967489750214 1104 30-31 0.13054878706618922 1104 32-33 -0.02389958822949012 1104 34-35 0.12973404276895195 1104 36-37 0.19062599824229665 1104 38-39 0.25524484643179335 1104 40-41 0.07108719983431655 1104 42-43 0.04328706674488103 1104 44-45 0.10384687649697355 1104 46-47 0.06785037268143412 1104 48-49 0.05691335398665842 1104 50-51 -0.2996297812847146 1104 52-53 -0.24896841352890675 1104 54-55 -0.050140119719991105 1104 56-57 -0.2547745997087745 1104 58-59 -0.029338312134441935 1104 60-61 -0.09448820459422791 1104 62-63 -0.07244611029852877 1104 64-65 -0.012083463618729695 1104 66-67 -0.4531765048657519 1104 68-69 -0.4114970761773904 1104 70-71 -0.16081454310536003 1104 72-73 0.26790118673098107 1104 74-75 0.14705360230844633 1104 76 0.4111654770154942 1105 1 -0.11761521371635553 1105 2 0.14900042662711144 1105 3 -0.037316823153425105 1105 4 0.10227956647854342 1105 5 0.1082893526103561 1105 6 0.09441812425425411 1105 7 0.07381170208629584 1105 8 0.11944943685832499 1105 9 0.06784758640386457 1105 10-11 -0.05336156607549469 1105 12-13 0.12793173058558693 1105 14-15 0.13117538246601157 1105 16-17 -0.04685221026773689 1105 18-19 -0.12344579764409502 1105 20-21 0.08140126354126664 1105 22-23 -0.0324159571216569 1105 24-25 0.11390773475586968 1105 26-27 0.0356887769573504 1105 28-29 0.0881307476583828 1105 30-31 0.23451407230253807 1105 32-33 0.15275436285874378 1105 34-35 0.11035513087680471 1105 36-37 -0.07616967599199143 1105 38-39 -0.010300874097083579 1105 40-41 0.007367337606986268 1105 42-43 0.12553093425596273 1105 44-45 -0.07603535008722417 1105 46-47 -0.057456714710383494 1105 48-49 -0.14306072104759693 1105 50-51 -0.23959496775928812 1105 52-53 -0.03713983927857356 1105 54-55 -0.24327494188707988 1105 56-57 -0.19958837203819257 1105 58-59 -0.05213376415474613 1105 60-61 -0.04530792732054323 1105 62-63 -0.27950325633015893 1105 64-65 -0.16441732779660612 1105 66-67 -0.3398874908786169 1105 68-69 -0.1489975391232008 1105 70-71 -0.09535709091223765 1105 72-73 -0.41871227202980776 1105 74-75 -0.8526038177871058 1105 76 -0.29109863665930646 1106 1 0.0117955729671948 1106 2 0.05527880338087954 1106 3 -0.017857564178378027 1106 4 -0.08033563514828757 1106 5 -0.2878597220676653 1106 6 -0.0509859607193377 1106 7 -0.3058580724344182 1106 8 -0.004647303092312427 1106 9 0.19169493065944465 1106 10-11 -0.11387199593135477 1106 12-13 -0.0975443594014962 1106 14-15 -0.23499185333442085 1106 16-17 0.05326097700923782 1106 18-19 0.11854156630735702 1106 20-21 0.19010581277453298 1106 22-23 0.11624945617177218 1106 24-25 -0.023464529884932972 1106 26-27 -0.24122357644274928 1106 28-29 0.09088688982672011 1106 30-31 0.16940900637254686 1106 32-33 0.09231747275910607 1106 34-35 -0.10693566450361658 1106 36-37 -0.1436312115021039 1106 38-39 -0.1393343698573517 1106 40-41 0.0026671879695712164 1106 42-43 0.12392584914168481 1106 44-45 0.10030659059496827 1106 46-47 0.008137550467580468 1106 48-49 0.06474344577894442 1106 50-51 0.11007698098030261 1106 52-53 0.1255077198409964 1106 54-55 0.1093385971017824 1106 56-57 0.09121020576687044 1106 58-59 0.14985756454878185 1106 60-61 0.194166295396208 1106 62-63 0.05655744274515939 1106 64-65 0.07317363768447649 1106 66-67 -0.09947323823438126 1106 68-69 -0.02628526501815287 1106 70-71 0.06899147474278067 1106 72-73 -0.2366246382194248 1106 74-75 0.024760645244775503 1106 76 0.3254522848258148 1107 1 -0.05913912127216747 1107 2 0.03509948797064055 1107 3 -0.0512006062085959 1107 4 0.1481585459653445 1107 5 0.08032572248512082 1107 6 0.1355793133825074 1107 7 0.19142560302495326 1107 8 0.18085423552508928 1107 9 0.0015902033683019567 1107 10-11 0.14314359285889822 1107 12-13 0.16503684540557373 1107 14-15 0.19876845160308676 1107 16-17 0.12418300773093449 1107 18-19 9.323118086399518E-4 1107 20-21 0.09008935020156628 1107 22-23 0.12885282213477467 1107 24-25 0.14786969833697583 1107 26-27 0.2047633548070138 1107 28-29 0.13752662541247673 1107 30-31 0.12221840776807369 1107 32-33 0.17549778858723641 1107 34-35 0.11956401257668148 1107 36-37 0.07672349362788111 1107 38-39 0.3125062724362664 1107 40-41 0.17785112556375537 1107 42-43 0.041597154876996 1107 44-45 0.027339401769253868 1107 46-47 0.17036354360395478 1107 48-49 0.2432895473150296 1107 50-51 0.3865722258294184 1107 52-53 0.11564917133339492 1107 54-55 0.417644598342811 1107 56-57 0.14656877326974893 1107 58-59 0.14921172514751646 1107 60-61 0.008193040745531732 1107 62-63 0.057383737273248414 1107 64-65 0.2606126945056815 1107 66-67 0.5637531727980729 1107 68-69 0.49102892951881216 1107 70-71 0.44031430579879327 1107 72-73 0.3813391947741067 1107 74-75 0.08815421471050655 1107 76 0.12774311517266312 1108 1 -0.366770202133047 1108 2 0.053067875641581 1108 3 0.04393721201424228 1108 4 0.11904687497423083 1108 5 -0.07607508474485059 1108 6 -0.02479497057693436 1108 7 -0.12959872668587025 1108 8 0.016794771255042917 1108 9 0.13216341185675162 1108 10-11 0.10883318535817921 1108 12-13 -0.11416883528733734 1108 14-15 0.16553602201635442 1108 16-17 0.12079239115264073 1108 18-19 0.12497811333535935 1108 20-21 0.09237691961206451 1108 22-23 0.10322264376702606 1108 24-25 0.06772642755143465 1108 26-27 -0.03702019725555772 1108 28-29 0.23603625827324493 1108 30-31 0.1647483968629473 1108 32-33 -0.13293550839567558 1108 34-35 -0.0723097887996218 1108 36-37 0.1384157936065833 1108 38-39 -0.0399047630492646 1108 40-41 0.13303670071511675 1108 42-43 0.1949927853060558 1108 44-45 0.008105016780724839 1108 46-47 0.006185000378295058 1108 48-49 0.10552459663878722 1108 50-51 0.12871614841556323 1108 52-53 0.07558850373916215 1108 54-55 0.15494388762946443 1108 56-57 0.2931863041989402 1108 58-59 0.25402627534242583 1108 60-61 0.2724449115385994 1108 62-63 0.10727468601916001 1108 64-65 0.12307650077048748 1108 66-67 -0.048795242198352184 1108 68-69 0.27361269216311257 1108 70-71 0.20086219434089259 1108 72-73 -0.020104167514219284 1108 74-75 -0.1916813309844727 1108 76 0.164087265889016 1109 1 0.08655239047374863 1109 2 0.10689937410079864 1109 3 0.06227316659631299 1109 4 0.12834271805733266 1109 5 0.1669658195220265 1109 6 -0.053098063650445226 1109 7 -0.016015543594846804 1109 8 0.09198625027865859 1109 9 0.12036139924918388 1109 10-11 0.12809297028791633 1109 12-13 0.22450289486469188 1109 14-15 0.14011310590909432 1109 16-17 0.10279528091953694 1109 18-19 0.2923895982408027 1109 20-21 0.15168427061644252 1109 22-23 -0.02858461133801171 1109 24-25 0.25765507843946267 1109 26-27 0.24889410709060655 1109 28-29 0.169651535678085 1109 30-31 0.03876542858644427 1109 32-33 0.22880001400002214 1109 34-35 -0.004566492163768032 1109 36-37 0.012838799219885288 1109 38-39 -0.007938315033101162 1109 40-41 0.04576829763099255 1109 42-43 0.1445689102053649 1109 44-45 0.09740648595867185 1109 46-47 0.11466633836593587 1109 48-49 0.11106438207998792 1109 50-51 0.1492897543587688 1109 52-53 0.1612713710016891 1109 54-55 0.07875185878294388 1109 56-57 0.10181916315018924 1109 58-59 0.17463440504948835 1109 60-61 -0.02325112590050793 1109 62-63 0.19970247381309747 1109 64-65 0.20100430774429157 1109 66-67 -3.852533226762489E-4 1109 68-69 0.20439254562891307 1109 70-71 0.16096806721672152 1109 72-73 -0.37554869294032756 1109 74-75 -0.40649266500828674 1109 76 -0.007304041794434113 1110 1 -0.16624244791572806 1110 2 -0.19527818033805744 1110 3 0.03187425847546166 1110 4 0.09850215722849498 1110 5 0.005783364034719796 1110 6 -0.24068414219129863 1110 7 -0.07109340202278958 1110 8 0.10574020523864647 1110 9 0.012476294714161895 1110 10-11 -0.16302620583963545 1110 12-13 -0.148491707778156 1110 14-15 -0.35936266802228545 1110 16-17 -0.030296101920590957 1110 18-19 -0.012030651154319116 1110 20-21 -0.06330115031537531 1110 22-23 -0.10468424531033804 1110 24-25 -0.22136036916894852 1110 26-27 0.004474478081363031 1110 28-29 0.05304690759942332 1110 30-31 -0.20670575448942685 1110 32-33 -0.031380518291065584 1110 34-35 -0.12562238893156064 1110 36-37 0.010388274993815116 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62-63 0.1229716346305878 2115 64-65 0.10012457395469454 2115 66-67 0.566302070696878 2115 68-69 0.4081930494886947 2115 70-71 0.2773003991175145 2115 72-73 0.6591657640631894 2115 74-75 0.5455295102109758 2115 76 0.6811246970741678 2116 1 0.0012188076660990532 2116 2 0.05082111044207949 2116 3 0.02954302653430574 2116 4 0.06218747375069 2116 5 0.08647025660103935 2116 6 0.024938395239750832 2116 7 -0.04954756540603 2116 8 0.11267479940728009 2116 9 -0.13865977042899402 2116 10-11 0.10600781044615104 2116 12-13 0.021760459282525346 2116 14-15 0.15543572966618058 2116 16-17 -0.3303946046786663 2116 18-19 0.13391103521289693 2116 20-21 0.07760376168882743 2116 22-23 0.17679066932060294 2116 24-25 -0.09719764261260622 2116 26-27 -0.15696497555667577 2116 28-29 -0.01653872413160684 2116 30-31 0.06522752630910134 2116 32-33 0.09950994664195179 2116 34-35 0.03042896383965399 2116 36-37 0.04355087021315285 2116 38-39 0.14292237137767216 2116 40-41 -0.22177286563213272 2116 42-43 -0.19959873653900218 2116 44-45 -0.23908314303736944 2116 46-47 0.025076099939767005 2116 48-49 -0.12664678011396546 2116 50-51 -0.008092903611945701 2116 52-53 0.12652984421883673 2116 54-55 0.19378179570157528 2116 56-57 -0.012776421154313766 2116 58-59 -0.12088574891180315 2116 60-61 -0.11097030691195187 2116 62-63 0.20176063696930413 2116 64-65 -0.10631687262564782 2116 66-67 0.18815051060333587 2116 68-69 0.11821030463671889 2116 70-71 0.17688541993776852 2116 72-73 0.3942243745246543 2116 74-75 0.6901875445501489 2116 76 0.6920838551370281 2117 1 -0.024833267095864642 2117 2 0.05628719719416608 2117 3 -0.06116168723082893 2117 4 -0.09183274489277693 2117 5 0.17605824296521178 2117 6 -0.04954438364822522 2117 7 -0.06848784987764134 2117 8 -0.4642618410064969 2117 9 0.05404917514523788 2117 10-11 -0.008640420422544537 2117 12-13 0.033603647245378454 2117 14-15 0.002921623635856463 2117 16-17 0.0956809801338423 2117 18-19 -0.06674724620241079 2117 20-21 -0.19974136585319968 2117 22-23 -0.04139729353352095 2117 24-25 -0.26008555844662595 2117 26-27 -0.13128024533450855 2117 28-29 -0.913381030467562 2117 30-31 0.08374169111455387 2117 32-33 0.03717892513025589 2117 34-35 -0.06198757548090583 2117 36-37 -0.19839693747463372 2117 38-39 -0.4442705285870545 2117 40-41 -0.06905304665952627 2117 42-43 -0.032559827405343356 2117 44-45 0.18860141085941962 2117 46-47 0.12914480806249173 2117 48-49 -0.009309587214005433 2117 50-51 -0.013735315743126364 2117 52-53 -0.10985636713652269 2117 54-55 -0.016295199713965758 2117 56-57 -0.20621270891733445 2117 58-59 -0.014737870693863897 2117 60-61 -0.18816408850358357 2117 62-63 -0.15453417906454803 2117 64-65 -0.13989216356252854 2117 66-67 0.20629733086899193 2117 68-69 -0.1213938448872014 2117 70-71 0.07915707857183207 2117 72-73 -0.4151458499963212 2117 74-75 -0.44370219331279515 2117 76 -0.7013127594156927 2118 1 0.0903029215548159 2118 2 -0.142994409686942 2118 3 -0.27378027847159103 2118 4 -0.2133072226080941 2118 5 -0.24022318673373633 2118 6 -0.11620514424291173 2118 7 -0.10645315438934233 2118 8 -0.18805096202191152 2118 9 0.009632829285664002 2118 10-11 -0.07383137446793597 2118 12-13 -0.29613235133716387 2118 14-15 -0.2843671671764483 2118 16-17 -0.15142772917466374 2118 18-19 -0.6222455307569561 2118 20-21 -0.47917776551172153 2118 22-23 -0.3032121220695103 2118 24-25 -0.3022911541813684 2118 26-27 -0.6592911339835723 2118 28-29 -0.4441060276487576 2118 30-31 -0.2232626417946193 2118 32-33 -0.4207121239492011 2118 34-35 -0.3321299655071641 2118 36-37 -0.22515144353909733 2118 38-39 -0.7956670461522606 2118 40-41 -0.342682703731505 2118 42-43 -0.34090510711154565 2118 44-45 -0.48583039817042106 2118 46-47 -0.38814655994485747 2118 48-49 -0.5068612519209239 2118 50-51 -0.372400328709638 2118 52-53 -0.31950715720787315 2118 54-55 -0.2014867980581201 2118 56-57 -0.5664602807163774 2118 58-59 -0.5521555212584346 2118 60-61 -0.5996535461651007 2118 62-63 -0.33110022774132375 2118 64-65 -0.6383015509954717 2118 66-67 -0.31605983614934985 2118 68-69 -0.8838328378482387 2118 70-71 -0.8857890419442427 2118 72-73 -1.0531385372053848 2118 74-75 -0.5752116242359122 2118 76 -0.9725383767344411 2119 1 0.07890219921897312 2119 2 0.14402530224900545 2119 3 0.031837405702290766 2119 4 0.020190014239737764 2119 5 -0.31509373196675483 2119 6 -0.1195122240044526 2119 7 -0.16897436756544693 2119 8 -0.13826200592774995 2119 9 -0.1804111200638019 2119 10-11 -0.040177486473503166 2119 12-13 -0.043958816678092205 2119 14-15 -0.49842660758373825 2119 16-17 -0.1267029565363984 2119 18-19 -0.217724404609271 2119 20-21 -0.05690719417017931 2119 22-23 -0.019978146176384826 2119 24-25 0.006942560626512773 2119 26-27 -0.0767490339879231 2119 28-29 0.1950959217877397 2119 30-31 -0.03215259436412765 2119 32-33 -0.140280463011905 2119 34-35 0.015579011473825233 2119 36-37 -0.03661743718602395 2119 38-39 0.08074390202231996 2119 40-41 -0.052334154930328225 2119 42-43 0.009610536246015045 2119 44-45 0.061278082748593476 2119 46-47 0.07264278777717692 2119 48-49 0.05096912969867162 2119 50-51 0.196225927763102 2119 52-53 -0.22047317261190358 2119 54-55 -0.30994160855374275 2119 56-57 -0.2814291680580894 2119 58-59 -0.20735764475175245 2119 60-61 -0.0903328029424344 2119 62-63 0.05258629590864672 2119 64-65 -0.20372081038366474 2119 66-67 -0.22371833664976748 2119 68-69 -0.08705724061574216 2119 70-71 0.2402150483912493 2119 72-73 0.16412354886571734 2119 74-75 0.2675454359442355 2119 76 -0.812989183922987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 0.0 18 0.0 19 0.0 20 2.0 21 5.0 22 7.0 23 16.0 24 26.0 25 61.0 26 128.0 27 228.0 28 418.0 29 687.0 30 1130.0 31 1757.0 32 2817.0 33 4777.0 34 7619.0 35 12391.0 36 19162.0 37 94426.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.77261805050186 16.734405250655644 11.724725040849112 31.76825165799338 2 21.60128520232325 19.867772453280974 33.08572134726551 25.445220997130264 3 19.43456590094605 19.72977797306018 31.278748850046 29.55690727594777 4 14.76884208213761 21.34040011533867 33.92329978442653 29.967458018097187 5 14.572491727196585 31.219706435623173 37.790577929121646 16.417223908058602 6 28.456384132694396 37.24203270675143 17.84042071152975 16.461162449024428 7 27.609194139697095 26.871850499114363 22.379134685358853 23.139820675829682 8 24.578121352757833 35.64308173941699 17.464883494212472 22.31391341361271 9 22.934545304754973 17.436048826703647 18.678685688393358 40.95072018014802 10-11 25.497398014527178 26.311290831948813 24.601463702645923 23.589847450878086 12-13 25.333658295459227 20.686814318472038 25.978319076192175 28.001208309876557 14-15 22.279243158631864 20.612324760740915 21.972703181424983 35.13572889920224 16-17 25.816638976232 26.054181713328482 21.629776599980776 26.499402710458746 18-19 27.09463263260514 24.914182537176124 22.534635927995716 25.456548902223012 20-21 23.81297285422016 23.750841011135673 22.422386686622087 30.013799448022077 22-23 22.9709319089923 26.593115379862418 22.237020966922515 28.198931744222765 24-25 25.423938266349943 26.191146383995385 23.8709854590891 24.51392989056557 26-27 26.717035796180095 27.057902758516526 22.87618943003474 23.348872015268643 28-29 23.985637589421795 26.65490395309561 23.331365252852574 26.028093204630025 30-31 22.946559749550318 24.705131197737167 28.538082357302724 23.810226695409796 32-33 25.04428181081712 22.93351549520109 26.21242911477571 25.809773579206087 34-35 25.706106084114843 22.33622595394692 24.119856101278337 27.8378118606599 36-37 28.070892089689547 24.486125032610637 23.70621593046726 23.736766947232557 38-39 22.449848274725728 25.073803018028535 27.504840104903266 24.971508602342475 40-41 21.889288607560175 21.66925263287976 28.851831001386813 27.589627758173258 42-43 24.738771643164124 21.392577132735585 28.462219720166416 25.406431503933874 44-45 21.047590932183606 24.703758118331983 30.418171332848175 23.83047961663623 46-47 22.98912521111096 22.46083290996718 29.17828062996883 25.371761248953028 48-49 22.38943278089772 22.19685839432094 27.08433453706628 28.32937428771506 50-51 21.24050858861168 22.613931263644975 28.94863309945214 27.196927048291204 52-53 18.219733897211277 22.832250889068913 33.984401817957135 24.96361339576268 54-55 18.52009501709484 23.480001098463525 30.980790619121507 27.019113265320133 56-57 20.977563882519327 24.26917848659188 27.057216218813934 27.696041412074862 58-59 19.102280684892005 24.16688407090582 30.365651045599968 26.365184198602204 60-61 21.0788284886515 26.67618668387593 27.239492509851843 25.005492317620725 62-63 18.50327479438136 31.484710760823297 24.348130552389843 25.663883892405497 64-65 18.36425050460668 33.00505293221107 23.08901673783795 25.5416798253443 66-67 18.51769212813577 32.7359293687954 22.555232119073445 26.191146383995385 68-69 18.2561205014486 33.03491740927378 22.482458910598798 26.226503178678822 70-71 18.644701973115104 32.27148525999258 22.697002567658487 26.386810199233825 72-73 18.866111027200702 30.35466641035851 23.451852970657296 27.32736959178349 74-75 19.379642724738773 29.054703483502454 23.708618819426327 27.85703497233245 76 19.354927295445496 29.20951818643672 23.669142786527345 27.76641173159044 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 2.0 15 4.5 16 5.5 17 6.0 18 10.0 19 24.5 20 35.5 21 36.0 22 42.5 23 65.5 24 120.5 25 159.0 26 241.0 27 388.0 28 509.0 29 565.0 30 669.5 31 886.0 32 1301.5 33 1605.0 34 1792.0 35 2309.0 36 2852.5 37 3066.0 38 3473.0 39 4539.0 40 5744.5 41 7848.0 42 9405.0 43 10562.5 44 11555.5 45 10708.5 46 10026.0 47 9594.0 48 8522.0 49 7414.0 50 6946.0 51 6513.0 52 5458.5 53 4531.0 54 4225.0 55 4067.5 56 3578.5 57 3133.5 58 3020.0 59 2886.5 60 2886.0 61 2961.5 62 2904.0 63 2911.0 64 2805.0 65 2676.0 66 2539.5 67 2419.0 68 2219.5 69 1819.0 70 1416.5 71 1215.0 72 975.5 73 573.5 74 306.5 75 202.0 76 129.5 77 35.5 78 9.0 79 4.0 80 2.5 81 0.5 82 0.0 83 0.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 145658.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.23443957764078 #Duplication Level Percentage of deduplicated Percentage of total 1 62.17503996523413 27.50278048579549 2 19.976408871505953 17.672905024097542 3 7.047849637596809 9.352730368397204 4 3.1180642858251466 5.517033050021283 5 1.8578013689062718 4.10894012000714 6 1.2897518275364344 3.42308695711873 7 0.925020564635036 2.8642436392096555 8 0.6425478418773571 2.2738194949813946 9 0.5696015892970775 2.2676406376580758 >10 2.36066489733203 15.459501022944158 >50 0.0217286709813599 0.5766933501764407 >100 0.009312287563439959 0.9233958999848961 >500 0.0 0.0 >1k 0.004656143781719979 3.8549204300484696 >5k 0.001552047927239993 4.202309519559516 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 6121 4.202309519559516 No Hit CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG 2067 1.4190775652555987 RNA PCR Primer, Index 18 (95% over 24bp) CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 1869 1.2831427041425805 No Hit TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 1679 1.1527001606502905 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 450 0.30894286616595035 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 258 0.17712724326847823 RNA PCR Primer, Index 30 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT 256 0.1757541638632962 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT 166 0.11396559063010614 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006178857323319007 0.0 2 0.0 0.0 0.0 0.006178857323319007 0.0 3 0.0 0.0 0.0 0.006865397025910008 0.0 4 0.0 0.0 0.0 0.03570006453473205 0.0 5 0.0 0.0 0.0 0.04393854096582405 0.0 6 0.0 0.0 0.0 0.04393854096582405 0.0 7 0.0 0.0 0.0 0.04393854096582405 0.0 8 0.0 0.0 0.0 0.04393854096582405 0.0 9 0.0 0.0 0.0 0.05080393799173406 0.0 10 0.0 0.0 0.0 0.059042414422826074 0.0 11 0.0 0.0 0.0 0.06041549382800807 0.0 12 0.0 0.0 0.0 0.06041549382800807 0.0 13 0.0 0.0 0.0 0.06316165263837208 0.0 14 0.0 0.0 0.0 0.06384819234096308 0.0 15 0.0 0.0 0.0 0.06384819234096308 0.0 16 0.0 0.0 0.0 0.06522127174614507 0.0 17 0.0 0.0 0.0 0.06659435115132709 0.0 18 0.0 0.0 0.0 0.06796743055650908 0.0 19 0.0 0.0 0.0 0.07277320847464609 0.0 20 0.0 0.0 0.0 0.07414628787982809 0.0 21 0.0 0.0 0.0 0.0789520657979651 0.0 22 0.0 0.0 0.0 0.08307130401351111 0.0 23 0.0 0.0 0.0 0.0851309231212841 0.0 24 0.0 0.0 0.0 0.0871905422290571 0.0 25 0.0 0.0 0.0 0.09405593925496711 0.0 26 0.0 0.0 0.0 0.10572711419901414 0.0 27 0.0 0.0 0.0 0.19840997404879923 0.0 28 0.0 0.0 0.0 0.2828543574674923 0.0 29 0.0 0.0 0.0 0.37210451880432244 0.0 30 0.0 0.0 0.0 0.4819508712188826 0.0 31 0.0 0.0 0.0 0.6062145573878538 0.0 32 0.0 0.0 0.0 0.7359705611775529 0.0 33 0.0 0.0 0.0 0.9158439632563952 0.0 34 0.0 0.0 0.0 1.1760425105383845 0.0 35 0.0 0.0 0.0 1.5639374425022998 0.0 36 0.0 0.0 0.0 2.1234673001139655 0.0 37 0.0 0.0 0.0 2.8422743687267435 0.0 38 0.0 0.0 0.0 3.6812258852929465 0.0 39 0.0 0.0 0.0 4.487223496134781 0.0 40 0.0 0.0 0.0 5.474467588460641 0.0 41 0.0 0.0 0.0 6.513888698183416 0.0 42 0.0 0.0 0.0 7.681006192588117 0.0 43 0.0 0.0 0.0 8.803498606324403 0.0 44 0.0 0.0 0.0 9.931483337681419 0.0 45 0.0 0.0 0.0 11.01758914718038 0.0 46 0.0 0.0 0.0 12.085844924411978 0.0 47 0.0 0.0 0.0 13.093685207815568 0.0 48 0.0 0.0 0.0 14.017767647503055 0.0 49 0.0 0.0 0.0 14.919194277005039 0.0 50 0.0 0.0 0.0 15.796592016916339 0.0 51 0.0 0.0 0.0 16.68978016998723 0.0 52 0.0 0.0 0.0 17.62347416551099 0.0 53 0.0 0.0 0.0 18.525587334715567 0.0 54 0.0 0.0 0.0 19.519010284364743 0.0 55 0.0 0.0 0.0 20.39434840516827 0.0 56 0.0 0.0 0.0 21.27792500240289 0.0 57 0.0 0.0 0.0 22.084609152947316 0.0 58 0.0 0.0 0.0 22.82881819055596 0.0 59 0.0 0.0 0.0 23.509865575526234 0.0 60 0.0 0.0 0.0 24.217688008897554 0.0 61 0.0 0.0 0.0 24.909033489406692 0.0 62 0.0 0.0 0.0 25.581842397945874 0.0 63 0.0 0.0 0.0 26.30682832388197 0.0 64 0.0 0.0 0.0 27.055156599706162 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTCA 20 0.0069399164 52.499996 55 CTTACAG 20 0.0069399164 52.499996 69 TCTTTAT 100 0.0 49.000004 1 CTTTATA 100 0.0 49.000004 2 GCTTTAC 45 1.6755966E-6 46.666664 1 TATTAAC 40 4.574975E-5 43.749996 2 ACGTTCT 35 0.0012521622 40.0 39 ATCACTC 35 0.0012521622 40.0 57 CCACGTT 35 0.0012521622 40.0 37 GTATAAG 45 9.162379E-5 38.888885 1 TTTATAC 165 0.0 36.060608 3 AGAACTC 50 1.7031736E-4 35.000004 17 ATAAGAG 50 1.7031736E-4 35.000004 3 TATAAGA 50 1.7031736E-4 35.000004 2 TATCACT 40 0.0024125273 34.999996 56 AAGAGAC 55 2.980728E-4 31.81818 5 TGATCAA 45 0.004296238 31.111109 48 CTACTGG 45 0.004296238 31.111109 9 TTGTATT 45 0.004296238 31.111109 37 GTGCTAG 45 0.004296238 31.111109 27 >>END_MODULE