FastQCFastQC Report
Wed 25 May 2016
SRR1780219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557481
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTT250.002345051435.22033739
TTAAACG300.005708621329.3687514
AAACTCG454.0795392E-529.31867829
CAATGTA406.963289E-427.54310216
AATGTAT450.001384574624.4827617
CCTTACG450.001392573624.45856734
TTACGAG450.001392573624.45856736
CTTACGA450.001392573624.45856735
CGAAACT450.001401343224.43223227
TCCGTAT551.5800973E-424.02681713
TATTAAG703.172739E-522.03448515
CCCAATG703.181561E-522.02656414
AAGTTGT654.8757915E-420.33952519
CCGTATT654.887632E-420.33221214
CGCAATC550.004440190720.0313517
CTAGATA550.004505289319.98104343
TATAGGT550.004505289319.9810435
TAATGCA708.128403E-418.85279723
TTACTCG951.5732154E-518.55535519
GGTATAG951.6076734E-518.5087551
Produced by FastQC (version 0.11.5)