FastQCFastQC Report
Wed 25 May 2016
SRR1780219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557481
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT135622.432728649048129No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC43650.7829863259913792No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG37660.6755387179114625No Hit
CACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCTG29910.5365205271569793TruSeq Adapter, Index 5 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC8070.1447582966953134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC353.213157E-431.4327353
ATGCCGT18600.030.63297344
CTTACAT300.005738674729.3372213
GTCAAAA300.00574370929.33195713
CTCGCTA300.00574370929.33195713
TATCGTG300.00574370929.33195737
GTCTTCT4050.028.24558843
TCTTCTG4100.027.90113344
CGTCTTC4150.027.56497242
CTTAATA655.9146987E-727.0805112
CATCGTT508.336176E-526.39876232
TATGCCG22250.025.6077943
CCGTCTT4550.025.14167841
TCTTTAT1600.024.7866361
GCCGTCT5000.023.31890740
CTTTATA1800.023.22532
TCTAGCA602.867518E-422.0029163
GGAGTTA500.002579412721.99896812
CGAGAAT602.871E-421.99896814
GACGTGT27650.020.44750426
Produced by FastQC (version 0.11.5)