FastQCFastQC Report
Wed 25 May 2016
SRR1780218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129146
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAGT150.002220421670.060
TCCAAGT150.002220421670.012
TTGCAAA150.002220421670.058
AGCAACG200.00693813752.546
AACGGCC200.00693813752.537
TCCGTTT200.00693813752.522
CCAAGGC200.00693813752.549
GACGCTT200.00693813752.514
ACGCTTC352.077096E-550.015
ATGGCTC305.859926E-446.6666647
TGTCAGG350.001251633540.029
AAGAGGC400.00241151435.067
CCGATGT400.00241151435.014
GGATAAT400.00241151435.06
AGAGGCG400.00241151435.068
TGCAAAG400.00241151435.059
GGCTAAG652.089676E-532.3076931
GACAGCG450.004294445331.11110937
AATATAC450.004294445331.1111091
TAAGAGG450.004294445331.1111094
Produced by FastQC (version 0.11.5)