FastQCFastQC Report
Wed 25 May 2016
SRR1780218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129146
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT78906.109364595109411No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC24561.901723630619609No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG21261.6461988756910784No Hit
CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG19811.5339228470103603Illumina PCR Primer Index 10 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC5130.3972248462979883No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT2270.1757700586932619Illumina PCR Primer Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT1550.1200192030724916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA650.064.615392
TCTTTGC252.3830678E-456.00000430
TCGTTCT200.00693813752.555
GTTGTAC200.00693813752.514
CTCGAAT200.00693813752.543
CACGTAG200.00693813752.568
ATACCTT200.00693813752.566
TCTTTAT950.044.2105261
TACCTCG404.5720684E-543.7536
ATCACGT350.001251633540.066
TACCGCA350.001251633540.07
TTTATAC1200.037.9166643
CTCGTAT16400.031.58536539
TCGTATG16400.031.58536540
TCTTCTG16450.031.48936350
CGTATGC16350.031.4678941
GTCTTCT16600.031.41566549
TATGCCG16400.031.37195243
GCCGTCT16400.031.37195246
CCGTCTT16450.031.27659647