##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780218_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 129146 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.75558670032366 34.0 34.0 34.0 34.0 34.0 2 33.74590773233395 34.0 34.0 34.0 34.0 34.0 3 33.778630387313584 34.0 34.0 34.0 34.0 34.0 4 33.797934121072274 34.0 34.0 34.0 34.0 34.0 5 33.80445387391015 34.0 34.0 34.0 34.0 34.0 6 37.58220153934307 38.0 38.0 38.0 37.0 38.0 7 37.584021185325135 38.0 38.0 38.0 37.0 38.0 8 37.50196676629551 38.0 38.0 38.0 37.0 38.0 9 37.51692657922042 38.0 38.0 38.0 37.0 38.0 10-11 37.465961005373764 38.0 38.0 38.0 37.0 38.0 12-13 37.46154352438326 38.0 38.0 38.0 37.0 38.0 14-15 37.38989206014898 38.0 38.0 38.0 37.0 38.0 16-17 37.42843758227123 38.0 38.0 38.0 37.0 38.0 18-19 37.390929645517474 38.0 38.0 38.0 36.5 38.0 20-21 37.40833630155018 38.0 38.0 38.0 37.0 38.0 22-23 37.39854118594459 38.0 38.0 38.0 37.0 38.0 24-25 37.41404302107692 38.0 38.0 38.0 37.0 38.0 26-27 37.33249965155716 38.0 38.0 38.0 36.5 38.0 28-29 37.290268378424415 38.0 38.0 38.0 36.5 38.0 30-31 37.28488300063494 38.0 38.0 38.0 37.0 38.0 32-33 37.264038375172284 38.0 38.0 38.0 36.0 38.0 34-35 37.22277112725133 38.0 38.0 38.0 36.0 38.0 36-37 37.19865113902095 38.0 38.0 38.0 36.0 38.0 38-39 36.831074907469066 38.0 38.0 38.0 35.0 38.0 40-41 37.057210443993625 38.0 38.0 38.0 36.0 38.0 42-43 37.209356077617585 38.0 38.0 38.0 36.0 38.0 44-45 37.25713533520202 38.0 38.0 38.0 36.5 38.0 46-47 37.21645656853484 38.0 38.0 38.0 36.5 38.0 48-49 37.22394808975888 38.0 38.0 38.0 36.0 38.0 50-51 37.11466479798058 38.0 38.0 38.0 36.0 38.0 52-53 37.10614343456243 38.0 38.0 38.0 36.0 38.0 54-55 37.080126368606074 38.0 38.0 38.0 36.0 38.0 56-57 37.1287689901352 38.0 38.0 38.0 36.0 38.0 58-59 37.041228532049004 38.0 38.0 38.0 36.0 38.0 60-61 37.03813900546668 38.0 38.0 38.0 36.0 38.0 62-63 37.02065491769005 38.0 38.0 38.0 36.0 38.0 64-65 36.95336673222555 38.0 38.0 38.0 35.5 38.0 66-67 36.67631595248788 38.0 38.0 38.0 34.5 38.0 68-69 36.59734331686619 38.0 38.0 38.0 34.0 38.0 70-71 35.976894367614946 38.0 37.5 38.0 31.5 38.0 72-73 34.910190791817016 38.0 37.0 38.0 24.0 38.0 74-75 34.564667120932896 38.0 37.0 38.0 23.0 38.0 76 33.982430737305066 38.0 35.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.023730020215985803 1101 2 -0.14728592072665236 1101 3 0.01814163225642318 1101 4 0.031650012309263786 1101 5 0.00507952739116746 1101 6 -0.0029665889237904253 1101 7 0.07706691894365036 1101 8 0.015625314816006153 1101 9 0.04874654096611408 1101 10-11 0.03202537449478626 1101 12-13 -0.04768949591370131 1101 14-15 0.0419893731989589 1101 16-17 -0.00665108194474584 1101 18-19 0.09138120668224303 1101 20-21 -0.004488552423666192 1101 22-23 0.053269230768947295 1101 24-25 -0.018535266929433192 1101 26-27 0.04518314700772663 1101 28-29 -0.23384123955138847 1101 30-31 -0.051830214269024566 1101 32-33 -0.2143378928271673 1101 34-35 0.1880713678846817 1101 36-37 0.16885511428172606 1101 38-39 0.31746591436424865 1101 40-41 0.017271982980886946 1101 42-43 0.0019491139938594415 1101 44-45 -0.10971893608982697 1101 46-47 0.09940374899424143 1101 48-49 0.191772262500983 1101 50-51 -0.06162341803117499 1101 52-53 0.10976294740457604 1101 54-55 0.03669445143013661 1101 56-57 0.07860671941731567 1101 58-59 -0.017607532078770305 1101 60-61 0.09662841317430093 1101 62-63 -0.044118018092753175 1101 64-65 -0.045312626070732165 1101 66-67 -0.20592121970839372 1101 68-69 -0.43986713372717645 1101 70-71 -0.15227051921610268 1101 72-73 -0.3099443089414038 1101 74-75 -0.05793550029648031 1101 76 -0.713753757952702 1102 1 -0.005161755069138962 1102 2 0.029375784722212472 1102 3 -0.11624686739871493 1102 4 -0.05462072497610393 1102 5 -0.21882870218010453 1102 6 -0.10956621864753657 1102 7 -0.03521391018474418 1102 8 0.03787819171730433 1102 9 -0.28490140617876136 1102 10-11 -0.11299949209685423 1102 12-13 -0.08896440788234372 1102 14-15 -0.2484052251239106 1102 16-17 -0.09871192341569213 1102 18-19 0.026564711238094674 1102 20-21 0.07093708376175556 1102 22-23 0.0065218535590929605 1102 24-25 -0.001900061643922868 1102 26-27 -0.16227138524624252 1102 28-29 -0.08727355523004832 1102 30-31 -0.002033503193409558 1102 32-33 -0.032539511878241 1102 34-35 0.1004393208378147 1102 36-37 0.057872067125948945 1102 38-39 -0.17560048125051964 1102 40-41 -0.18826764020857212 1102 42-43 0.03433013552070463 1102 44-45 -0.09001624614173664 1102 46-47 -0.030156638706777983 1102 48-49 -0.17718805985716557 1102 50-51 -0.04172106931806496 1102 52-53 -0.15109122909376538 1102 54-55 0.05298479157607261 1102 56-57 -0.03141433550700157 1102 58-59 -0.07277687901309804 1102 60-61 0.019678110055991738 1102 62-63 -0.024787419479480377 1102 64-65 0.06270276580497125 1102 66-67 0.021326756477762387 1102 68-69 0.005272082884431484 1102 70-71 0.05046480586466373 1102 72-73 0.07491611143105104 1102 74-75 -0.3259410907418996 1102 76 -0.24557210477812674 1103 1 0.10476744423311146 1103 2 0.08639257851091031 1103 3 -0.00701883442679474 1103 4 -0.08665205513993612 1103 5 0.024920656509223704 1103 6 -0.10330702652795765 1103 7 0.006975780960033262 1103 8 0.0017595748961483082 1103 9 -0.04746254446244791 1103 10-11 -0.08800547766309563 1103 12-13 0.09898237188302517 1103 14-15 0.028726491536488652 1103 16-17 0.087199763829922 1103 18-19 0.08170887787625958 1103 20-21 0.01763989184740211 1103 22-23 0.20981733531166924 1103 24-25 0.1545027840652864 1103 26-27 0.020040411449727458 1103 28-29 0.07174583800455281 1103 30-31 0.17519911301208424 1103 32-33 0.15438972162724696 1103 34-35 -0.15210026940741983 1103 36-37 0.003574430569528886 1103 38-39 -0.030108467706035924 1103 40-41 0.19515063115989761 1103 42-43 -0.06479084132371327 1103 44-45 -0.005867735692646647 1103 46-47 -0.07059341402572272 1103 48-49 0.02217404979840154 1103 50-51 0.11095362370789985 1103 52-53 0.15037608971458383 1103 54-55 -0.12271722983515332 1103 56-57 -0.237805210083728 1103 58-59 0.0886196390975158 1103 60-61 -0.03491880481215759 1103 62-63 0.11452235922558884 1103 64-65 0.04288199075965338 1103 66-67 -0.13121112360568787 1103 68-69 -0.01618189666203307 1103 70-71 -0.4503218685547381 1103 72-73 -0.1491407343465596 1103 74-75 -0.22646097992341652 1103 76 -0.5092961530730946 1104 1 -0.16008061039285337 1104 2 0.05529363655099928 1104 3 -0.03321631270824099 1104 4 0.03882541502874659 1104 5 -0.015119231264158373 1104 6 0.01348302181061456 1104 7 -0.10305362782203531 1104 8 -0.29910578696708257 1104 9 -0.14140163110003812 1104 10-11 0.007099819297998522 1104 12-13 -0.042137528059512874 1104 14-15 -0.1447684210822473 1104 16-17 -0.14209577752829006 1104 18-19 -0.05884481850341672 1104 20-21 -0.04885944899023542 1104 22-23 -0.05072926239647302 1104 24-25 -0.1647430665921874 1104 26-27 0.012553003140901353 1104 28-29 -0.028032751049970273 1104 30-31 0.012326855296507233 1104 32-33 0.017427615011960995 1104 34-35 0.09549970355001847 1104 36-37 0.021633788984836144 1104 38-39 -0.10419662644585514 1104 40-41 -0.11858529825902053 1104 42-43 -0.009962054520478603 1104 44-45 -0.10812240898474101 1104 46-47 -0.08058225897045901 1104 48-49 0.026729030699598866 1104 50-51 -0.19183006962948923 1104 52-53 -0.10531974911176434 1104 54-55 -0.4627943011261024 1104 56-57 -0.3850857428221346 1104 58-59 0.07977165207793746 1104 60-61 0.10390247768116723 1104 62-63 -0.22039972440352074 1104 64-65 -0.24703113502204133 1104 66-67 -0.5156295395878416 1104 68-69 -0.24753943308498094 1104 70-71 -0.031804476809469406 1104 72-73 0.4067528531868234 1104 74-75 0.2243807197013723 1104 76 0.14587742634751777 1105 1 -0.028984754811872904 1105 2 -0.15754643464147478 1105 3 -0.08887357179799693 1105 4 -0.08164242122856535 1105 5 -0.016161692489689017 1105 6 -0.03233442034550649 1105 7 0.0115916811024519 1105 8 0.03516830405757787 1105 9 0.08722791377664407 1105 10-11 0.1710713423084229 1105 12-13 0.02958888943349791 1105 14-15 -0.00919760999656205 1105 16-17 0.014794636913613601 1105 18-19 -0.07486312513957927 1105 20-21 0.06673933056735137 1105 22-23 -0.1362746475986114 1105 24-25 -0.05541176732953801 1105 26-27 -0.06877168539202927 1105 28-29 -0.030155812326349007 1105 30-31 -0.08581705520438021 1105 32-33 -0.12266900334552133 1105 34-35 -0.00994143552956217 1105 36-37 -0.1365152066927635 1105 38-39 0.012762437881626454 1105 40-41 -0.2387607368555109 1105 42-43 -0.196130373869579 1105 44-45 -0.3471511402331444 1105 46-47 -0.2803152871931829 1105 48-49 -0.188653628847824 1105 50-51 -0.3419826249307931 1105 52-53 -0.01407896599454972 1105 54-55 -0.2765624170681278 1105 56-57 0.06047744049849513 1105 58-59 0.01505006550280541 1105 60-61 -0.1421819362522143 1105 62-63 -0.15181340352886963 1105 64-65 -0.5586761896876951 1105 66-67 -0.49640757162445937 1105 68-69 0.039162829818657485 1105 70-71 0.025136789399525128 1105 72-73 0.7661604301001219 1105 74-75 0.1486751514328688 1105 76 0.04872512530506157 1106 1 -0.07266120718397673 1106 2 0.14278801351905912 1106 3 -0.03494070916947578 1106 4 -0.18534612493227343 1106 5 0.03593789874033604 1106 6 0.09205552143574636 1106 7 0.02132783866469623 1106 8 0.03985057830507088 1106 9 0.059827938550583326 1106 10-11 0.0034261050979651486 1106 12-13 0.13375091098681935 1106 14-15 0.07835348625018668 1106 16-17 0.18520216819070612 1106 18-19 0.06628638557515387 1106 20-21 0.06266401779638642 1106 22-23 0.11297594078241247 1106 24-25 0.22032216057830567 1106 26-27 0.07759273923326049 1106 28-29 0.29049326484446425 1106 30-31 0.12463754655456682 1106 32-33 0.14420824179258318 1106 34-35 -0.10145636182709694 1106 36-37 -0.00453205297047532 1106 38-39 0.061195486314680636 1106 40-41 0.14243584434107248 1106 42-43 0.0878037274558281 1106 44-45 0.09588118975570126 1106 46-47 -0.004296459000443065 1106 48-49 0.19646962149281677 1106 50-51 0.023532053627363325 1106 52-53 0.12526204725951118 1106 54-55 0.3299407416089437 1106 56-57 0.220777205146085 1106 58-59 0.16527922442762133 1106 60-61 0.3013458724929805 1106 62-63 0.2995058086599087 1106 64-65 0.34968501432568644 1106 66-67 0.17637825768310478 1106 68-69 0.25598433359668604 1106 70-71 0.4409425617270415 1106 72-73 0.23007718966905344 1106 74-75 0.051003901587705514 1106 76 0.44914628224795194 1107 1 0.005952672616842847 1107 2 0.15039662646891827 1107 3 0.06669345181186515 1107 4 -0.010821451891274592 1107 5 -0.09840484391100546 1107 6 0.04319131826886746 1107 7 0.07027658197545605 1107 8 0.15230587770399495 1107 9 0.1697893037048246 1107 10-11 0.009720118810385259 1107 12-13 0.057537971272395794 1107 14-15 0.20304597147679715 1107 16-17 0.23314065678201956 1107 18-19 -0.02261180272956409 1107 20-21 0.06943145631456815 1107 22-23 0.13825767081191742 1107 24-25 -0.06295918056653704 1107 26-27 0.18246901473268196 1107 28-29 0.21594576596462645 1107 30-31 0.025480411789125412 1107 32-33 0.0467714989552519 1107 34-35 0.0019150991111231974 1107 36-37 0.11491471632792383 1107 38-39 0.28031931226342977 1107 40-41 0.2209820920267802 1107 42-43 0.2607010027269894 1107 44-45 0.12414718686442683 1107 46-47 0.1766279029328146 1107 48-49 0.10787155069890275 1107 50-51 0.26119976013779933 1107 52-53 0.15739998295826751 1107 54-55 0.15120174239516615 1107 56-57 0.15735096219462008 1107 58-59 0.3113688200721114 1107 60-61 -0.008366944638233065 1107 62-63 0.053772972422621024 1107 64-65 0.17540973470915588 1107 66-67 0.23545068322437857 1107 68-69 0.21926432209922098 1107 70-71 -0.014716132804061033 1107 72-73 -0.07344306192241135 1107 74-75 -0.07869336015703254 1107 76 -0.4212959472447224 1108 1 -0.18563512785020464 1108 2 -0.08009167089908686 1108 3 -0.06003384964723324 1108 4 -0.14633670130746168 1108 5 -0.056106808688980436 1108 6 -0.014891662434514785 1108 7 -0.029296750421430318 1108 8 0.06746399053386654 1108 9 -0.13697241209592192 1108 10-11 -0.15294032049922635 1108 12-13 -0.20582831320778894 1108 14-15 -0.02799102394047992 1108 16-17 -0.10167050329734906 1108 18-19 -0.08438203432402958 1108 20-21 0.008806906246967117 1108 22-23 -0.10886276182552024 1108 24-25 -0.0033793515847477806 1108 26-27 -0.2569459414592572 1108 28-29 -0.03253982741939154 1108 30-31 0.008321526392393253 1108 32-33 -0.2544330030025037 1108 34-35 -0.028258904014258235 1108 36-37 0.057872067125948945 1108 38-39 -0.2776714871676802 1108 40-41 -0.016670007072470128 1108 42-43 -0.3147822905148061 1108 44-45 0.09785357634347491 1108 46-47 -0.0020501298310406924 1108 48-49 -0.09730640305243554 1108 50-51 -0.01953172020563443 1108 52-53 -0.27387229418252446 1108 54-55 -0.3612163918558835 1108 56-57 -0.08466877337681211 1108 58-59 -0.6437827961728644 1108 60-61 -0.07203786627537312 1108 62-63 -0.15644422421320314 1108 64-65 0.16921164154462076 1108 66-67 0.18848651979137543 1108 68-69 0.2168105444228985 1108 70-71 0.09780208397117462 1108 72-73 -0.07893004241510226 1108 74-75 0.2391476666545458 1108 76 0.4023568893047127 1109 1 -0.02397969920014731 1109 2 -0.0831362560098654 1109 3 0.1078655586367887 1109 4 0.05711726869391498 1109 5 0.07914501462187218 1109 6 -0.21385615947593806 1109 7 0.01914139461778319 1109 8 -0.024251985797491216 1109 9 0.10060843281944898 1109 10-11 -0.1671827864411526 1109 12-13 -0.04166153186747579 1109 14-15 -0.11710964235248156 1109 16-17 -0.10308958957779879 1109 18-19 0.084515034617759 1109 20-21 0.05217074276823297 1109 22-23 0.049051439357910454 1109 24-25 0.029964875744269648 1109 26-27 0.08172544976132912 1109 28-29 -0.055615030391734877 1109 30-31 -0.06928099184072067 1109 32-33 -0.04959434881232738 1109 34-35 -6.770270361400321E-4 1109 36-37 -0.04050071393913868 1109 38-39 -0.13016391870751676 1109 40-41 -0.07635763608031709 1109 42-43 0.0031618404883602125 1109 44-45 0.031964970316231245 1109 46-47 0.04362724735349133 1109 48-49 -0.15652100330013496 1109 50-51 0.016401264519828374 1109 52-53 0.02399821634450916 1109 54-55 -0.3862439135291993 1109 56-57 0.07706405997944898 1109 58-59 -0.05022630793837379 1109 60-61 -0.16140589985180753 1109 62-63 -0.32812786257841253 1109 64-65 -0.17681319676555063 1109 66-67 -0.11808771992239997 1109 68-69 -0.13918229994204268 1109 70-71 0.23737136978375162 1109 72-73 -0.2826936312413082 1109 74-75 0.11029463239006532 1109 76 0.18557210879006192 1110 1 0.06536806877632984 1110 2 0.061444070106148274 1110 3 -0.037249383891946763 1110 4 -0.0075044901940870545 1110 5 5.519906453343992E-5 1110 6 -0.30149983763686095 1110 7 -0.07114195208095708 1110 8 -0.024251985797491216 1110 9 0.07915694925890193 1110 10-11 -0.10887618392845866 1110 12-13 -0.03928917153601219 1110 14-15 -0.13458491634339254 1110 16-17 -0.05659466843371774 1110 18-19 0.057383251672014524 1110 20-21 0.08839100473026917 1110 22-23 -0.027816376734044468 1110 24-25 0.03622991183088686 1110 26-27 0.042397729900322645 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0.34214911024157857 2115 44-45 0.2629007145733908 2115 46-47 0.3109757645286564 2115 48-49 0.28830422250172916 2115 50-51 0.2898615447275006 2115 52-53 0.42100945309865523 2115 54-55 0.32543199856809224 2115 56-57 0.29963446748958233 2115 58-59 0.3722632020538015 2115 60-61 0.3424211750361792 2115 62-63 0.13032992847485048 2115 64-65 0.23335944387167018 2115 66-67 0.330982403112948 2115 68-69 0.43117187116953914 2115 70-71 0.12806193233264196 2115 72-73 0.13770676702340268 2115 74-75 -0.02151498285778075 2115 76 0.31587045172441464 2116 1 0.04868614425186735 2116 2 0.1834052134282942 2116 3 0.1679688034745439 2116 4 0.15552572036435208 2116 5 -0.07305536954496716 2116 6 0.08371021220547448 2116 7 0.16080291619287124 2116 8 0.29285715874528506 2116 9 0.16869514476292125 2116 10-11 0.15291051121954524 2116 12-13 0.18575151608477825 2116 14-15 0.3500046472818781 2116 16-17 0.13481515762670426 2116 18-19 0.11379918087555296 2116 20-21 0.14323028862813914 2116 22-23 0.21479627711602234 2116 24-25 0.08387257280128324 2116 26-27 0.1631636743617193 2116 28-29 0.16439337325785175 2116 30-31 0.29509868161746766 2116 32-33 0.1777029345793153 2116 34-35 0.25442947635962554 2116 36-37 0.11916005302251165 2116 38-39 0.14993057947454957 2116 40-41 -0.21752179880241584 2116 42-43 -0.06533647878171678 2116 44-45 -0.03005482113606206 2116 46-47 0.11088645706742994 2116 48-49 0.02468483466378757 2116 50-51 0.06864966954144336 2116 52-53 0.06288653355655782 2116 54-55 0.1470877047736039 2116 56-57 0.1644148523235529 2116 58-59 0.19873609859246244 2116 60-61 0.04313292843933425 2116 62-63 0.15965254491283787 2116 64-65 0.06717990881172398 2116 66-67 0.25684367885649095 2116 68-69 0.17307330820778333 2116 70-71 0.4589190133318084 2116 72-73 0.62357995042845 2116 74-75 0.2822521682342085 2116 76 0.713775785815514 2117 1 -0.017991152513921804 2117 2 -0.1059992352681931 2117 3 -0.010377684606453386 2117 4 -0.03419497378111913 2117 5 -0.07794521514277619 2117 6 0.029762459142681053 2117 7 -0.002927185590330339 2117 8 -0.26599372753923234 2117 9 -0.18233730361465916 2117 10-11 0.048509650181514985 2117 12-13 -0.04134726411135148 2117 14-15 0.1290580369547385 2117 16-17 -0.05165006866153021 2117 18-19 -0.10064978136101388 2117 20-21 -0.055300100167734456 2117 22-23 0.07551540332187301 2117 24-25 -0.10065266039651988 2117 26-27 -0.0950414334009011 2117 28-29 -0.6820947077435093 2117 30-31 -0.2261748042577878 2117 32-33 0.19460479218913918 2117 34-35 0.08838732801658722 2117 36-37 -0.2991343475727746 2117 38-39 0.08333300845220037 2117 40-41 0.041719743624966554 2117 42-43 0.06716770681980933 2117 44-45 -0.04237244849793598 2117 46-47 0.05222159751760813 2117 48-49 -0.08161615659295762 2117 50-51 0.28837381846913246 2117 52-53 -0.06308014108267912 2117 54-55 0.030448942117139666 2117 56-57 -0.11528963092074918 2117 58-59 0.08123364038203817 2117 60-61 -0.04715647600936279 2117 62-63 -0.00864849949439872 2117 64-65 -0.02915536451469336 2117 66-67 -0.009307428002578888 2117 68-69 -0.056058478446132654 2117 70-71 0.10052522130969521 2117 72-73 0.18757314716500417 2117 74-75 0.7164385670223723 2117 76 0.41180124490291803 2118 1 -0.12274769477046732 2118 2 0.06975687798792052 2118 3 -0.1897534889822623 2118 4 -0.032237659323804735 2118 5 0.03166439446683711 2118 6 -0.008499016618792155 2118 7 -0.0944383396014814 2118 8 -0.056990081035586115 2118 9 0.07659126781391024 2118 10-11 0.18525353692539426 2118 12-13 0.05610286458878022 2118 14-15 0.16016056805727175 2118 16-17 0.0937542642428042 2118 18-19 0.05589515643392673 2118 20-21 0.003928771561142241 2118 22-23 0.08178953459600535 2118 24-25 -0.029143651387506964 2118 26-27 0.05455905994958954 2118 28-29 0.21591044026164496 2118 30-31 -0.010829995162971784 2118 32-33 -0.09517159133724817 2118 34-35 -0.04511224298298089 2118 36-37 0.08896357177514602 2118 38-39 -0.0021185144993225435 2118 40-41 0.08653688219211375 2118 42-43 0.17443122033331804 2118 44-45 0.16399016411467926 2118 46-47 0.09148854303455067 2118 48-49 0.25772318274638195 2118 50-51 0.10907845167376706 2118 52-53 -0.05012969063223238 2118 54-55 0.05059855601675878 2118 56-57 -0.012879184760308249 2118 58-59 -0.017972708824316896 2118 60-61 -0.12288845799134407 2118 62-63 0.14917003332412548 2118 64-65 0.010513768904438336 2118 66-67 -0.17415084284598947 2118 68-69 -0.3958268182144664 2118 70-71 -0.3704636246791466 2118 72-73 -0.3551258017839345 2118 74-75 -0.22580725022343984 2118 76 -0.5921133840117108 2119 1 0.11414097961820602 2119 2 0.16727403800508256 2119 3 0.1667994925707248 2119 4 0.0168560772699351 2119 5 -0.014882152079714217 2119 6 0.07781050719072624 2119 7 0.03010584744270517 2119 8 0.034306572761067855 2119 9 0.13598101470365975 2119 10-11 0.1911522928241638 2119 12-13 0.017211294447207592 2119 14-15 -0.05862965073294646 2119 16-17 0.16306464605318638 2119 18-19 0.16361448290324887 2119 20-21 0.15791311304548117 2119 22-23 0.05749738530386139 2119 24-25 0.04949748975363377 2119 26-27 0.0716252332657632 2119 28-29 0.09117856552976633 2119 30-31 0.19574711766413344 2119 32-33 0.03844863603298165 2119 34-35 0.1754744151036789 2119 36-37 0.22338817494974705 2119 38-39 0.1433930685122604 2119 40-41 0.18436238626761536 2119 42-43 -0.039438899786794934 2119 44-45 -0.009339415464900469 2119 46-47 0.06723661253262492 2119 48-49 0.22318864821184548 2119 50-51 0.1772627073580182 2119 52-53 0.10058352258098324 2119 54-55 0.32174023340843405 2119 56-57 0.2240497084185833 2119 58-59 0.2839363430847399 2119 60-61 0.3207113918585023 2119 62-63 0.3366968458509447 2119 64-65 0.23060439524506648 2119 66-67 0.2339358152406703 2119 68-69 0.23523281284516884 2119 70-71 0.32725194803641955 2119 72-73 0.22811368770555163 2119 74-75 0.3725947231785369 2119 76 -0.038649205547535814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 3.0 21 2.0 22 2.0 23 11.0 24 22.0 25 47.0 26 81.0 27 153.0 28 263.0 29 491.0 30 804.0 31 1280.0 32 2087.0 33 3552.0 34 6018.0 35 10486.0 36 17247.0 37 86596.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.47577160732814 17.058213185077353 12.811856348628684 33.65415885896582 2 19.364904836386724 19.055952178154957 36.23573320118316 25.34340978427516 3 18.281634738977594 19.168228206835675 32.83105942112029 29.719077633066455 4 14.825856007929012 23.047558577114273 33.160918650209844 28.965666764746878 5 15.144874792870086 30.772923667786845 38.31012962073932 15.77207191860375 6 28.25639199046041 37.558267387298095 17.34471063757298 16.840629984668514 7 26.845585616279248 25.928019450854073 22.245365710126524 24.981029222740155 8 23.323989902900593 36.883836897774614 16.895606522850105 22.896566676474688 9 22.30808542269989 16.47747510569433 17.829433354498008 43.38500611710777 10-11 26.07552692301736 26.329503043067536 23.993000170349838 23.601969863565266 12-13 25.127762377464265 19.71334768401654 25.674817648243074 29.48407229027612 14-15 22.399842039242408 19.182940238180045 21.06530593282022 37.35191178975733 16-17 27.004320691310607 25.39451473526087 20.081148467625788 27.520016105802735 18-19 28.765892865439113 23.955058615830147 20.923218682731175 26.355829835999568 20-21 24.21213200563703 22.202778250971768 20.576324469979713 33.00876527341149 22-23 22.9712883093553 26.063525002710108 20.602264104192155 30.36292258374243 24-25 27.897883016121288 25.66126709305747 21.198101373639137 25.242748517182108 26-27 28.287364688027505 27.505691233177952 21.06491877410063 23.14202530469391 28-29 23.76264073219457 27.254425224164898 22.536509067257214 26.44642497638332 30-31 26.723630619608812 23.794387747200844 25.16725256686231 24.31472906632803 32-33 26.373251978381056 25.6225512210986 24.395258080002478 23.608938720517862 34-35 24.645749771576355 26.53624579932789 25.805290136744457 23.01271429235129 36-37 23.227587381723012 25.140925773930277 28.70627042262246 22.92521642172425 38-39 21.437365462344943 24.101404611834667 28.511916745388937 25.94931318043145 40-41 21.902343084570948 20.319638238892416 28.602124727053102 29.175893949483527 42-43 25.81961500936924 20.401715887445217 27.70701376736407 26.071655335821475 44-45 21.412200145571678 24.868753194059437 29.90530097718861 23.813745683180276 46-47 24.375512985303455 21.836526102240875 27.66558778436808 26.122373128087588 48-49 23.94847691759714 20.758289068186393 24.98141638145974 30.31181763275673 50-51 22.12805661809115 21.242237467672247 28.185929103495265 28.443776810741333 52-53 18.897991420562775 21.340188623728185 34.40098802905239 25.36083192665665 54-55 19.37497096309603 22.200842457373827 30.631223576417387 27.79296300311276 56-57 22.58103232000991 22.98793613429762 26.017840273798647 28.413191271893822 58-59 20.028494881761727 23.06691651309371 30.453904882845773 26.450683722298795 60-61 22.83694423365803 26.31672680532111 26.31208090068605 24.534248060334814 62-63 19.359871773032072 33.11716971489631 22.722345252659782 24.800613259411826 64-65 18.514704288169977 34.92984684001053 21.641785266287766 24.913663605531724 66-67 18.804686169141902 34.54810834249609 21.321992163907517 25.32521332445449 68-69 18.57045514379075 35.29145308410636 21.294891053536308 24.84320071856658 70-71 18.88134359562046 34.26548247719635 21.58138850603193 25.271785421151254 72-73 19.17016400043362 32.04202995059855 22.36422343704025 26.423582611927586 74-75 19.447756802378706 31.17363294256113 23.080854226998902 26.297756028061265 76 19.656048193517414 31.551887011599277 23.00497111795952 25.78709367692379 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.5 15 3.0 16 4.0 17 5.0 18 10.0 19 16.0 20 20.0 21 23.0 22 37.5 23 70.0 24 92.5 25 97.0 26 161.5 27 283.5 28 425.0 29 509.0 30 635.5 31 822.0 32 1056.0 33 1230.0 34 1443.5 35 1891.0 36 2439.0 37 2753.0 38 4925.0 39 8439.0 40 9280.5 41 8066.5 42 7353.0 43 6954.0 44 6519.0 45 6367.0 46 6251.0 47 6326.5 48 6175.5 49 5636.5 50 5324.0 51 4936.5 52 3971.5 53 3249.5 54 3105.0 55 3063.5 56 2821.5 57 2562.5 58 2504.0 59 2479.5 60 2453.0 61 2483.5 62 2516.0 63 2541.5 64 2626.5 65 2783.5 66 2785.5 67 2690.0 68 2661.5 69 2514.0 70 2173.0 71 1951.0 72 1776.5 73 1356.0 74 902.0 75 694.0 76 540.5 77 251.5 78 80.5 79 45.0 80 24.0 81 4.0 82 2.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 129146.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.61146299536958 #Duplication Level Percentage of deduplicated Percentage of total 1 60.57494866529775 25.811871834977467 2 20.57930984354273 17.538289997367322 3 7.622976140720684 9.74478497204714 4 3.572531845686976 6.089232341690799 5 1.8952953789682179 4.038065445309959 6 1.2647416910468645 3.2335496260046765 7 1.059402882011957 3.159989469282827 8 0.7050571496065854 2.4034813312065415 9 0.5651360142465156 2.167314512257445 >10 2.1333430248405443 13.438279156923173 >50 0.012720103214551798 0.41116256020318087 >100 0.005451472806236485 0.3755439580010221 >500 0.0018171576020788284 0.3972248462979883 >1k 0.005451472806236485 5.0818453533210475 >5k 0.0018171576020788284 6.109364595109411 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 7890 6.109364595109411 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 2456 1.901723630619609 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 2126 1.6461988756910784 No Hit CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG 1981 1.5339228470103603 Illumina PCR Primer Index 10 (96% over 26bp) TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 513 0.3972248462979883 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 227 0.1757700586932619 Illumina PCR Primer Index 10 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT 155 0.1200192030724916 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004645904635064191 0.0 2 0.0 0.0 0.0 0.006968856952596286 0.0 3 0.0 0.0 0.0 0.006968856952596286 0.0 4 0.0 0.0 0.0 0.026326792932030416 0.0 5 0.0 0.0 0.0 0.029424062688739875 0.0 6 0.0 0.0 0.0 0.03019838012791724 0.0 7 0.0 0.0 0.0 0.03019838012791724 0.0 8 0.0 0.0 0.0 0.03019838012791724 0.0 9 0.0 0.0 0.0 0.03174701500627197 0.0 10 0.0 0.0 0.0 0.03484428476298143 0.0 11 0.0 0.0 0.0 0.03484428476298143 0.0 12 0.0 0.0 0.0 0.035618602202158796 0.0 13 0.0 0.0 0.0 0.03639291964133616 0.0 14 0.0 0.0 0.0 0.038715871958868255 7.743174391773651E-4 15 0.0 0.0 0.0 0.041038824276400356 7.743174391773651E-4 16 0.0 0.0 0.0 0.041038824276400356 7.743174391773651E-4 17 0.0 0.0 0.0 0.04336177659393245 7.743174391773651E-4 18 0.0 0.0 0.0 0.044136094033109814 7.743174391773651E-4 19 0.0 0.0 0.0 0.04955631610735137 7.743174391773651E-4 20 0.0 0.0 0.0 0.05420222074241556 7.743174391773651E-4 21 0.0 0.0 0.0 0.058073807938302385 7.743174391773651E-4 22 0.0 0.0 0.0 0.0665912997692534 7.743174391773651E-4 23 0.0 0.0 0.0 0.07433447416102705 7.743174391773651E-4 24 0.0 0.0 0.0 0.07820606135691388 7.743174391773651E-4 25 0.0 0.0 0.0 0.08130333111362334 7.743174391773651E-4 26 0.0 0.0 0.0 0.09678967989717065 7.743174391773651E-4 27 0.0 0.0 0.0 0.1711241540581977 7.743174391773651E-4 28 0.0 0.0 0.0 0.2570733898068852 7.743174391773651E-4 29 0.0 0.0 0.0 0.3825128149536184 7.743174391773651E-4 30 0.0 0.0 0.0 0.4816254471683211 7.743174391773651E-4 31 0.0 0.0 0.0 0.6062905548758769 7.743174391773651E-4 32 0.0 0.0 0.0 0.786706518204203 7.743174391773651E-4 33 0.0 0.0 0.0 0.9919006395862048 7.743174391773651E-4 34 0.0 0.0 0.0 1.2977560280612639 7.743174391773651E-4 35 0.0 0.0 0.0 1.6934322394808976 7.743174391773651E-4 36 0.0 0.0 0.0 2.199061527263717 7.743174391773651E-4 37 0.0 0.0 0.0 2.8974958574017005 7.743174391773651E-4 38 0.0 0.0 0.0 3.645486503647035 7.743174391773651E-4 39 0.0 0.0 0.0 4.391154197574838 7.743174391773651E-4 40 0.0 0.0 0.0 5.13991916125935 7.743174391773651E-4 41 0.0 0.0 0.0 5.961469964226534 7.743174391773651E-4 42 0.0 0.0 0.0 6.8031530206123305 7.743174391773651E-4 43 0.0 0.0 0.0 7.6649683304167375 7.743174391773651E-4 44 0.0 0.0 0.0 8.51052297399842 7.743174391773651E-4 45 0.0 0.0 0.0 9.486937264801078 7.743174391773651E-4 46 0.0 0.0 0.0 10.411472287178851 7.743174391773651E-4 47 0.0 0.0 0.0 11.260898517956422 7.743174391773651E-4 48 0.0 0.0 0.0 12.043733448964737 7.743174391773651E-4 49 0.0 0.0 0.0 12.805661809115264 7.743174391773651E-4 50 0.0 0.0 0.0 13.493255695104764 7.743174391773651E-4 51 0.0 0.0 0.0 14.22343704024902 7.743174391773651E-4 52 0.0 0.0 0.0 15.004723336378982 7.743174391773651E-4 53 0.0 0.0 0.0 15.748068077989252 7.743174391773651E-4 54 0.0 0.0 0.0 16.450373995323123 7.743174391773651E-4 55 0.0 0.0 0.0 17.220045529865423 7.743174391773651E-4 56 0.0 0.0 0.0 17.915382590246697 7.743174391773651E-4 57 0.0 0.0 0.0 18.57277809610828 7.743174391773651E-4 58 0.0 0.0 0.0 19.267340839050377 7.743174391773651E-4 59 0.0 0.0 0.0 19.89531228222322 7.743174391773651E-4 60 0.0 0.0 0.0 20.527929630031128 7.743174391773651E-4 61 0.0 0.0 0.0 21.180679231257646 7.743174391773651E-4 62 0.0 0.0 0.0 21.8698217521255 7.743174391773651E-4 63 0.0 0.0 0.0 22.50166478249423 7.743174391773651E-4 64 0.0 0.0 0.0 23.112601242005173 7.743174391773651E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTATA 65 0.0 64.61539 2 TCTTTGC 25 2.3830678E-4 56.000004 30 TCGTTCT 20 0.006938137 52.5 55 GTTGTAC 20 0.006938137 52.5 14 CTCGAAT 20 0.006938137 52.5 43 CACGTAG 20 0.006938137 52.5 68 ATACCTT 20 0.006938137 52.5 66 TCTTTAT 95 0.0 44.210526 1 TACCTCG 40 4.5720684E-5 43.75 36 ATCACGT 35 0.0012516335 40.0 66 TACCGCA 35 0.0012516335 40.0 7 TTTATAC 120 0.0 37.916664 3 CTCGTAT 1640 0.0 31.585365 39 TCGTATG 1640 0.0 31.585365 40 TCTTCTG 1645 0.0 31.489363 50 CGTATGC 1635 0.0 31.46789 41 GTCTTCT 1660 0.0 31.415665 49 TATGCCG 1640 0.0 31.371952 43 GCCGTCT 1640 0.0 31.371952 46 CCGTCTT 1645 0.0 31.276596 47 >>END_MODULE