FastQCFastQC Report
Wed 25 May 2016
SRR1780217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596226
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTAG300.00577356629.3011821
CATAAGC703.610876E-828.26419431
TTATGCA450.001406338924.4176522
TTCGGTA500.002586554321.98879826
CTTAATA755.513021E-520.5108262
AAATTCA654.8672385E-420.34515817
CGTATTA550.00443343620.03689615
TCCGTAT550.00445289920.02173813
TAGGTTC550.00448549319.99652710
TAGATCT1553.274181E-1119.8825439
GTATAAG1404.125468E-918.8364731
TTCAGTA600.007347250418.35943414
CGTAAGC600.007347250418.35943414
GCATAAG600.007429597418.32399730
ATAAGCC600.00744399518.31784832
TATAAGA1203.1918535E-718.3132382
ATATAGC1203.1946547E-718.3117056
TATTGGC750.001273985117.6324720
GTTGGCA750.001294240417.59103828
ACGAGAG800.00198460216.50267624
Produced by FastQC (version 0.11.5)