FastQCFastQC Report
Wed 25 May 2016
SRR1780217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596226
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT182183.0555527601949595No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC60441.0137095665066602No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG47670.7995290376467984No Hit
CACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTTCTG39590.6640099559562984RNA PCR Primer, Index 34 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC10490.1759399959075921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG4500.037.1541444
GCTAGTC301.3012775E-436.6621937
GTATACC250.00232913935.269651
CCGTCTT4750.034.73551641
GTCTTCT4800.034.3736943
ATGCCGT23750.032.51244444
CGTCTTC5100.032.35170742
GATCATA300.005687488229.3913781
CTTATAT300.005687488229.3913781
TAAGTGC300.0057390729.3371374
ATAGACC300.0057390729.3371374
GTATCAA300.00574613229.32975634
CGAGAAT300.00574613229.32975616
GCCGTCT5750.028.6945640
CTTTATA1550.028.3907782
TATGCCG28600.026.92205243
AAGTGCT701.1310349E-625.14195
ACTCGCA450.001397010924.44556221
AGCTAGT450.001397693824.4435146
AAGTTGT450.00139837724.44146219
Produced by FastQC (version 0.11.5)