FastQCFastQC Report
Wed 25 May 2016
SRR1780216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316430
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC308.369367E-658.32951420
GATTACG252.3891532E-455.99633419
TTAATAC350.001253819240.00372
GTCTATA350.001253819240.00371
GATATTA350.001253819240.00371
TTAGATC701.9217623E-839.9973838
AGATCTA501.7083458E-434.9977140
TGTATAA400.002417569734.99770711
GCAATTT400.002417569734.99770713
GATCCTA450.004301839531.113991
CGAAAGC450.004305168531.10907237
GGCAATT450.004305168531.10907212
ATCCGAC450.004305168531.10907210
AGCAAGC703.480735E-530.0027756
AGTAACC703.480735E-530.00277569
CATCCGA604.978136E-429.1647579
CGTGCGA500.007204899627.99816710
CCTGCAA500.007204899627.99816713
TAGATCT2650.027.73403439
GCGACTC657.942224E-426.9255743
Produced by FastQC (version 0.11.5)