##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780216_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 316430 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.22048478336441 34.0 33.0 34.0 21.0 34.0 2 31.122241886041145 34.0 33.0 34.0 21.0 34.0 3 30.99270296748096 34.0 33.0 34.0 21.0 34.0 4 30.86850804285308 34.0 32.0 34.0 21.0 34.0 5 30.90979047498657 34.0 33.0 34.0 21.0 34.0 6 33.97313149827766 38.0 34.0 38.0 22.0 38.0 7 34.35685617672155 38.0 35.0 38.0 24.0 38.0 8 34.22740258508991 38.0 35.0 38.0 23.0 38.0 9 34.518332648611064 38.0 36.0 38.0 24.0 38.0 10-11 34.31064058401542 38.0 35.5 38.0 23.0 38.0 12-13 34.37682899851468 38.0 36.0 38.0 23.0 38.0 14-15 34.353642195746296 38.0 36.0 38.0 23.0 38.0 16-17 34.424856998388265 38.0 36.0 38.0 23.0 38.0 18-19 34.42505767468319 38.0 36.0 38.0 23.0 38.0 20-21 34.45156906740827 38.0 36.0 38.0 23.5 38.0 22-23 34.44923995828461 38.0 36.0 38.0 23.0 38.0 24-25 34.48483234838669 38.0 36.0 38.0 23.0 38.0 26-27 34.48799734538444 38.0 36.0 38.0 23.0 38.0 28-29 34.593235470720224 38.0 36.0 38.0 24.0 38.0 30-31 34.5976598299782 38.0 36.0 38.0 24.0 38.0 32-33 34.66116992699807 38.0 36.0 38.0 24.5 38.0 34-35 34.60549410612142 38.0 36.0 38.0 24.0 38.0 36-37 34.55525234649053 38.0 36.0 38.0 23.5 38.0 38-39 34.5534715418892 38.0 36.0 38.0 24.0 38.0 40-41 34.590000948076984 38.0 36.0 38.0 24.0 38.0 42-43 34.64601491641122 38.0 36.0 38.0 24.0 38.0 44-45 34.68560661125683 38.0 36.0 38.0 24.5 38.0 46-47 34.63009354359574 38.0 36.0 38.0 24.0 38.0 48-49 34.59872325632841 38.0 36.0 38.0 24.0 38.0 50-51 34.54358783933255 38.0 36.0 38.0 24.0 38.0 52-53 34.57829219732642 38.0 36.0 38.0 24.0 38.0 54-55 34.562958632240935 38.0 36.0 38.0 24.0 38.0 56-57 34.51914167430395 38.0 36.0 38.0 24.0 38.0 58-59 34.578734633252225 38.0 36.0 38.0 24.0 38.0 60-61 34.58908605378757 38.0 36.0 38.0 24.0 38.0 62-63 34.48223303732263 38.0 36.0 38.0 24.0 38.0 64-65 34.473558132920395 38.0 36.0 38.0 24.0 38.0 66-67 34.39067566286383 38.0 36.0 38.0 23.5 38.0 68-69 34.34131877508453 38.0 36.0 38.0 23.0 38.0 70-71 34.365962456151436 38.0 36.0 38.0 23.5 38.0 72-73 34.40542142021933 38.0 36.0 38.0 23.0 38.0 74-75 34.43524318174636 38.0 36.0 38.0 24.0 38.0 76 32.98306734506842 37.0 32.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3852372375317188 1101 2 -0.08079602926814289 1101 3 0.03268719332879044 1101 4 0.18625325756714162 1101 5 -0.34872980184150393 1101 6 -0.12082592754191523 1101 7 -0.6342236892783575 1101 8 -0.8939524739543572 1101 9 -1.1226106010299546 1101 10-11 -0.9671657035271224 1101 12-13 -0.49175234014183644 1101 14-15 -0.5259962057691965 1101 16-17 -0.58930122062214 1101 18-19 -0.5628371138818622 1101 20-21 -0.6040887970012321 1101 22-23 -0.5937093936670053 1101 24-25 -0.8026837041466308 1101 26-27 -0.7481394103303316 1101 28-29 -0.7402592080543684 1101 30-31 -0.6617753666530319 1101 32-33 -0.7257042137118148 1101 34-35 -0.5162920048225388 1101 36-37 -0.6382094755062937 1101 38-39 -0.4738630870298053 1101 40-41 -0.32399548872244566 1101 42-43 -0.7381998567697963 1101 44-45 -0.5047899139131999 1101 46-47 -0.29191757158179854 1101 48-49 -0.8545232668870071 1101 50-51 -0.8440745857699952 1101 52-53 -0.4693843088557017 1101 54-55 -0.4793453461857169 1101 56-57 -0.6620482894707749 1101 58-59 -0.653867025422052 1101 60-61 -0.712756186080874 1101 62-63 -0.7677412757506374 1101 64-65 -0.45914828457152623 1101 66-67 -0.6949851571696826 1101 68-69 -0.5624778437661107 1101 70-71 -0.3131579891558687 1101 72-73 -0.46724917934985655 1101 74-75 -0.6704081581369437 1101 76 -1.2699205468104218 1102 1 0.22409615933868565 1102 2 -0.030680173394042498 1102 3 0.24606601523240101 1102 4 0.13504287640429524 1102 5 -0.30949516540259836 1102 6 -0.3700590152647649 1102 7 -0.5360261565021673 1102 8 -0.16683475366438927 1102 9 -0.2061608538876314 1102 10-11 0.05860105983502706 1102 12-13 0.2483105615563943 1102 14-15 0.18768640274516457 1102 16-17 -0.04344880134896556 1102 18-19 -0.25366052451200716 1102 20-21 0.08345103776267848 1102 22-23 -0.2166394750937286 1102 24-25 0.06929629954645833 1102 26-27 -0.01003027350126473 1102 28-29 0.06115667581575224 1102 30-31 0.15238615251828946 1102 32-33 0.19353247775416804 1102 34-35 0.01614689535080771 1102 36-37 -0.06207965736129495 1102 38-39 -0.014200439082749483 1102 40-41 -0.06086192983251948 1102 42-43 0.08488390494237308 1102 44-45 0.12269771124986306 1102 46-47 -0.3692811505454685 1102 48-49 -0.11249766541930484 1102 50-51 -0.11696282208023945 1102 52-53 0.0441892938748083 1102 54-55 -0.26186689418698705 1102 56-57 0.09415848794894544 1102 58-59 -0.016078204769321758 1102 60-61 0.07664895803907257 1102 62-63 0.06972097429553514 1102 64-65 0.07600756987299206 1102 66-67 0.27666365955168715 1102 68-69 0.21581526266046325 1102 70-71 0.19359456175817513 1102 72-73 0.04167008489088886 1102 74-75 -0.10108301115783291 1102 76 -0.20880285034685642 1103 1 0.26099643003736617 1103 2 0.45508557530463634 1103 3 0.19158735069895982 1103 4 0.466403814392244 1103 5 0.27079163607566414 1103 6 -0.44196658994371063 1103 7 0.31282215307411576 1103 8 0.37831450061921146 1103 9 0.15764396481529275 1103 10-11 0.43279721499612833 1103 12-13 0.3952456279138232 1103 14-15 0.114646600122839 1103 16-17 0.36945844920197857 1103 18-19 0.35849664352689814 1103 20-21 0.5199811513258865 1103 22-23 0.2695451239421729 1103 24-25 0.407894616152916 1103 26-27 0.43277297488290145 1103 28-29 0.6672360738552996 1103 30-31 0.25858839358959784 1103 32-33 0.19849356019367548 1103 34-35 -0.0069786753882823405 1103 36-37 0.22835901066503794 1103 38-39 -0.09722456811824998 1103 40-41 0.12239841303274801 1103 42-43 0.06297936433830387 1103 44-45 0.14348926438539422 1103 46-47 0.17565497847819955 1103 48-49 0.3689182559256423 1103 50-51 -0.18054075118353552 1103 52-53 0.38382881617820885 1103 54-55 0.0930147842928406 1103 56-57 0.29424832616615504 1103 58-59 0.1500706957855371 1103 60-61 0.281873782987752 1103 62-63 0.12458074823474874 1103 64-65 0.4319467344515715 1103 66-67 0.387656038843609 1103 68-69 0.3698552443672156 1103 70-71 0.4216785508710217 1103 72-73 0.38031354261142525 1103 74-75 0.3693029379673902 1103 76 0.3830963177246147 1104 1 -0.21446548055306636 1104 2 -0.3605989849331692 1104 3 -0.08755090190930304 1104 4 -0.05381242387784724 1104 5 -0.4439268461764776 1104 6 -0.21068635447130646 1104 7 0.1261621892109659 1104 8 -0.06505263815796525 1104 9 0.2162235533214414 1104 10-11 -0.01858195968475229 1104 12-13 -0.019379597941508564 1104 14-15 0.16396684841799214 1104 16-17 -0.1988045046943938 1104 18-19 -0.03475829614787784 1104 20-21 -0.3286782879700283 1104 22-23 -0.03732597823186978 1104 24-25 -0.2489480719627224 1104 26-27 -0.16919852363082555 1104 28-29 -0.37993490591971835 1104 30-31 -0.039813133483903584 1104 32-33 -0.31357367183989027 1104 34-35 -0.14747016574207095 1104 36-37 -0.22782359701696464 1104 38-39 -0.14124814450106982 1104 40-41 -0.1862081324006013 1104 42-43 -0.1558722705628952 1104 44-45 -0.0587102751611468 1104 46-47 -0.24592003463597933 1104 48-49 -0.5632260583484197 1104 50-51 -0.34943172862714533 1104 52-53 -0.22697872593288082 1104 54-55 -0.29558097836963526 1104 56-57 -0.21330034201592696 1104 58-59 -0.31306242772090087 1104 60-61 -0.24974304979187423 1104 62-63 -0.21529874701499807 1104 64-65 -0.03934122381618721 1104 66-67 -0.17891372859824628 1104 68-69 -0.21806076659041196 1104 70-71 -0.06348639638082432 1104 72-73 -0.1624051727817104 1104 74-75 -0.26375791183151875 1104 76 -0.11815535797627064 1105 1 -0.10094303808325478 1105 2 -0.25051696169325055 1105 3 -0.01857733161994446 1105 4 0.20711261599456066 1105 5 -0.10433354474107404 1105 6 0.3205793096890943 1105 7 -0.048894771372310686 1105 8 -0.3107919865288977 1105 9 0.0929950274613418 1105 10-11 -0.006406635009426509 1105 12-13 -0.7857101971668499 1105 14-15 -0.06924401966129778 1105 16-17 -0.16062667375340567 1105 18-19 -0.024556655683078077 1105 20-21 -0.02436067575768419 1105 22-23 0.21459074539538392 1105 24-25 -0.007344061937658353 1105 26-27 0.1534945727464816 1105 28-29 -0.138664115306824 1105 30-31 -0.10184890473019692 1105 32-33 -0.27626164176470525 1105 34-35 0.061606503203016416 1105 36-37 -0.158741801619378 1105 38-39 0.11001922847683687 1105 40-41 -0.1609831380966682 1105 42-43 0.013240853155494392 1105 44-45 -0.1251348593488899 1105 46-47 0.13730792161829442 1105 48-49 0.09996031668006111 1105 50-51 0.10474633015618195 1105 52-53 0.0072431924995655095 1105 54-55 0.09980236635581718 1105 56-57 -0.08153057669353103 1105 58-59 0.030968103151735704 1105 60-61 -0.07108048428720082 1105 62-63 -0.005384757495747294 1105 64-65 -0.33041834844594575 1105 66-67 -0.0014615754882072451 1105 68-69 -0.12068664947945251 1105 70-71 -0.03703959756560948 1105 72-73 0.09459528290295793 1105 74-75 0.02687776827784205 1105 76 -0.26720411623555407 1106 1 -0.3762351529610335 1106 2 -0.22648888699328396 1106 3 -0.23943371177083606 1106 4 -0.21776041442158345 1106 5 -0.17188192891879694 1106 6 -0.23398084957431564 1106 7 -0.8011174460473924 1106 8 -0.24571795392966322 1106 9 -0.14721785160932654 1106 10-11 -0.6725628782933342 1106 12-13 -0.3941094189479273 1106 14-15 -0.4613919830578581 1106 16-17 -0.21993334630195704 1106 18-19 -0.2880595457257229 1106 20-21 -0.39205214815240197 1106 22-23 -0.40127819032237966 1106 24-25 -0.17090011519135828 1106 26-27 -0.46577907074328806 1106 28-29 -0.3740880636367123 1106 30-31 -0.5040059737405684 1106 32-33 -0.3257359480979076 1106 34-35 -0.35572328968668643 1106 36-37 -0.24422852778359783 1106 38-39 -0.30595179191747945 1106 40-41 -0.35450853766909773 1106 42-43 -0.43343474945515936 1106 44-45 -0.41415184895377877 1106 46-47 -0.32401442842186867 1106 48-49 -0.0471335220431186 1106 50-51 -0.3228824209608163 1106 52-53 -0.39242114403486994 1106 54-55 -0.4374967162960033 1106 56-57 -0.4310772754843555 1106 58-59 -0.40309113423897003 1106 60-61 -0.32898992584726017 1106 62-63 -0.36244075984849644 1106 64-65 -0.25386756626806317 1106 66-67 -0.5834941068488462 1106 68-69 -0.34289190471941566 1106 70-71 -0.4697089563267909 1106 72-73 -0.2691174321535428 1106 74-75 -0.5069105629629007 1106 76 -0.10880885341699553 1107 1 -0.21231660656303575 1107 2 -0.3945168119917888 1107 3 -0.3640379227104056 1107 4 -0.3882889581738773 1107 5 -0.2402264851656497 1107 6 -0.8616361567748996 1107 7 -0.21834099112253824 1107 8 -0.1405468791756732 1107 9 0.1593127745695071 1107 10-11 0.02432030952617481 1107 12-13 0.20328242715734035 1107 14-15 -0.25892080844980825 1107 16-17 0.11535012827793167 1107 18-19 -0.00294206785149953 1107 20-21 0.09902499550942423 1107 22-23 0.13006915671741837 1107 24-25 -0.12053995781453608 1107 26-27 -0.04782582292599358 1107 28-29 0.1995841760813093 1107 30-31 0.018322503442348648 1107 32-33 -0.1695496904061642 1107 34-35 0.1239175770806824 1107 36-37 0.19012809629574434 1107 38-39 0.09823311731773998 1107 40-41 0.26093171631866596 1107 42-43 0.27821531754094053 1107 44-45 -0.1440828899110187 1107 46-47 0.08272230677712145 1107 48-49 -0.08569468478816589 1107 50-51 -0.06837405284924358 1107 52-53 0.08162106777521672 1107 54-55 0.10133968520265313 1107 56-57 0.22967069116268135 1107 58-59 0.13819681243725768 1107 60-61 0.02650759487032417 1107 62-63 0.2061491642794806 1107 64-65 0.41761897903752043 1107 66-67 0.17778001388069242 1107 68-69 0.3913384082419782 1107 70-71 0.20185226616000307 1107 72-73 -0.02712495961340977 1107 74-75 0.09191081775416876 1107 76 0.028221640476544962 1108 1 -0.09473269535265416 1108 2 0.06211427303188799 1108 3 -0.06777529382434366 1108 4 0.20601971041471856 1108 5 0.3220803480681269 1108 6 0.17865074727586716 1108 7 0.3209806201804781 1108 8 0.22132968455529323 1108 9 -0.026616460381880813 1108 10-11 0.009916669982935389 1108 12-13 -0.10448579869519392 1108 14-15 0.11726849281675555 1108 16-17 0.0036835048979355633 1108 18-19 -0.031409546576099956 1108 20-21 -0.11631103891213712 1108 22-23 -0.06442001728633784 1108 24-25 -0.16085283386748728 1108 26-27 0.008747690826176324 1108 28-29 -0.06392342938666218 1108 30-31 -0.09951679912281719 1108 32-33 -0.4097495882496176 1108 34-35 -0.03873857958243576 1108 36-37 -0.23276535789397457 1108 38-39 0.10556155059999384 1108 40-41 0.0966241731834927 1108 42-43 0.13527623920072074 1108 44-45 -0.2934781751268929 1108 46-47 -0.113944186742863 1108 48-49 -0.14190026184277116 1108 50-51 -0.13595536685941312 1108 52-53 -0.12450356320591993 1108 54-55 0.024036185042497493 1108 56-57 -0.10809736154315885 1108 58-59 -0.21634266067739105 1108 60-61 0.006303541506518684 1108 62-63 -0.030667880279814597 1108 64-65 -0.025565101856614092 1108 66-67 0.24647595965114988 1108 68-69 -0.12500663846433469 1108 70-71 0.08239404212551449 1108 72-73 -0.13269465548125936 1108 74-75 -0.08964862783015093 1108 76 -0.20437495372824088 1109 1 0.15976120654609005 1109 2 -0.042652449942533366 1109 3 0.057759558389236076 1109 4 0.44533338462433747 1109 5 0.15020307039086234 1109 6 0.3015717191991598 1109 7 0.5092528867533943 1109 8 0.12105043239087365 1109 9 0.5436910599903797 1109 10-11 0.2809348971175609 1109 12-13 0.03286939462333294 1109 14-15 0.36123613157821666 1109 16-17 0.38072966296552124 1109 18-19 0.33518466315742046 1109 20-21 0.37942943664544515 1109 22-23 0.528705720502451 1109 24-25 0.14764935127355727 1109 26-27 0.35263444078258743 1109 28-29 0.16020287147967593 1109 30-31 0.6443758102700059 1109 32-33 0.33219297212401244 1109 34-35 0.39895060161135376 1109 36-37 0.14024922209876678 1109 38-39 0.5356170294588622 1109 40-41 0.3124772356679486 1109 42-43 0.48652301293786593 1109 44-45 0.5332054124488224 1109 46-47 0.5065765599494085 1109 48-49 0.3801362210090886 1109 50-51 0.6412395637277015 1109 52-53 0.3449251846182335 1109 54-55 0.6558613566096554 1109 56-57 0.4878794922538958 1109 58-59 0.5043122813999332 1109 60-61 0.6469678872647151 1109 62-63 0.49119353404522315 1109 64-65 0.3604381336955882 1109 66-67 0.5704456277783834 1109 68-69 0.4054209860629783 1109 70-71 0.6168203747185572 1109 72-73 0.26752700271065777 1109 74-75 0.5808402492445879 1109 76 0.4227848202404303 1110 1 0.08896489633702842 1110 2 0.31235294465383134 1110 3 0.276593760695075 1110 4 -0.28619788991260364 1110 5 -0.12982732011012743 1110 6 0.36853391183426254 1110 7 -0.05944489722550372 1110 8 -0.1060027622834454 1110 9 -0.09062591632423533 1110 10-11 -0.0910684187233457 1110 12-13 0.22606161171771078 1110 14-15 0.16747903528325736 1110 16-17 0.012787122477810442 1110 18-19 0.03629789884197265 1110 20-21 -0.028808055516854836 1110 22-23 0.023571674668154685 1110 24-25 0.3660034062588977 1110 26-27 -0.07532580866806171 1110 28-29 -0.09818417245097777 1110 30-31 -0.1021805449034403 1110 32-33 0.027430051924497434 1110 34-35 -0.05480203213792123 1110 36-37 0.37910486157026213 1110 38-39 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2115 72-73 0.09202409892697716 2115 74-75 0.1067427884485852 2115 76 0.15435864762597618 2116 1 -0.2051312281548796 2116 2 0.4069867465850123 2116 3 -0.23704398020214157 2116 4 -0.43786952078174934 2116 5 -0.6125336013031379 2116 6 -0.033810887758690455 2116 7 0.019460195989069007 2116 8 -0.057157296667931234 2116 9 -0.8643583296126707 2116 10-11 0.06433851248480948 2116 12-13 -0.09934829229500508 2116 14-15 0.036823560463489 2116 16-17 2.5560789105583126E-4 2116 18-19 0.04811694079852913 2116 20-21 -0.2078988060928353 2116 22-23 0.08379169078990145 2116 24-25 -0.10938520218272174 2116 26-27 -0.05098811522137936 2116 28-29 -0.24913689914161807 2116 30-31 -0.15033211791723744 2116 32-33 -0.044839827276739186 2116 34-35 -0.09630338419230355 2116 36-37 -0.38048457696986304 2116 38-39 -0.10290231762990487 2116 40-41 -0.5162838116469715 2116 42-43 0.002952906069879191 2116 44-45 -0.1253087299833595 2116 46-47 -0.27210692072937803 2116 48-49 -0.30955841517534566 2116 50-51 -0.07330946428111673 2116 52-53 -0.27148874948390755 2116 54-55 -0.04725611766539117 2116 56-57 0.03300688413146702 2116 58-59 -0.030129749221273983 2116 60-61 -0.14389837175178144 2116 62-63 -0.1559293116654672 2116 64-65 -0.41924027028640154 2116 66-67 -0.29249937395410086 2116 68-69 -0.1063441257724449 2116 70-71 -0.12735709544452334 2116 72-73 -0.13243647842821105 2116 74-75 -0.16267657459216878 2116 76 0.100586384800458 2117 1 0.2785097022649232 2117 2 -0.06937424836381112 2117 3 0.445582339295278 2117 4 0.11811421860502236 2117 5 -0.2478264834195265 2117 6 0.10394340311491845 2117 7 0.23906453273604455 2117 8 0.29628495613005157 2117 9 0.15175933958274612 2117 10-11 0.357875545929339 2117 12-13 0.07600100895663076 2117 14-15 0.32009269053235556 2117 16-17 -0.04344880134896556 2117 18-19 0.028080708861629944 2117 20-21 -0.45887060673550906 2117 22-23 -0.07093209903569431 2117 24-25 0.04511250269035827 2117 26-27 0.01475811827624085 2117 28-29 0.0013017785968898465 2117 30-31 0.049605015879834014 2117 32-33 -0.03500440253603898 2117 34-35 0.20054834637861774 2117 36-37 -0.10561049170228642 2117 38-39 0.17987453068750625 2117 40-41 0.28859393473822337 2117 42-43 0.259007242306339 2117 44-45 0.06102902926679121 2117 46-47 0.1815048228523537 2117 48-49 0.07794973482253909 2117 50-51 0.26211819363923183 2117 52-53 0.0284698259183358 2117 54-55 0.08335680593393135 2117 56-57 -0.11200838024936388 2117 58-59 0.16167270212306306 2117 60-61 0.1473865638431775 2117 62-63 0.2589591846945609 2117 64-65 0.03791808982462186 2117 66-67 0.2276914709180744 2117 68-69 0.02053110304741068 2117 70-71 0.17243373950908136 2117 72-73 -1.075621329861228 2117 74-75 -0.13373113691357474 2117 76 -0.12657794103609632 2118 1 -0.029248189753250387 2118 2 -0.1444408712022387 2118 3 0.4023127134397484 2118 4 0.07790278647813764 2118 5 0.17694694023128932 2118 6 0.34945003017963927 2118 7 0.18879510645553665 2118 8 0.11000515194836424 2118 9 -0.048432017644806535 2118 10-11 -0.3502393799344077 2118 12-13 -0.11720668994335881 2118 14-15 0.1556450731059229 2118 16-17 -0.0990876762154258 2118 18-19 -0.07042057076645847 2118 20-21 0.07925629362959796 2118 22-23 0.025064220566100914 2118 24-25 0.1314206289156843 2118 26-27 -0.08474984124524809 2118 28-29 -0.09921946440331197 2118 30-31 -0.04339034106595818 2118 32-33 0.023527576426410235 2118 34-35 -0.2267755286639428 2118 36-37 0.09682388333440173 2118 38-39 -0.12578929105677616 2118 40-41 0.02113699011442094 2118 42-43 -0.03510250634653289 2118 44-45 -0.00971004400071962 2118 46-47 -0.25500463935165385 2118 48-49 0.002544861670074283 2118 50-51 0.11084913128964757 2118 52-53 0.009960710035684883 2118 54-55 -0.06844274443528064 2118 56-57 0.04293521045748605 2118 58-59 0.0030032218398972077 2118 60-61 -0.2079376868700038 2118 62-63 -0.12676430411162443 2118 64-65 -0.08407654512918583 2118 66-67 -0.2069477091807812 2118 68-69 -0.17604735928573945 2118 70-71 -0.061134339053040776 2118 72-73 -0.13763633320704116 2118 74-75 -0.3628043750770189 2118 76 0.22720987549575966 2119 1 0.37245083937296286 2119 2 -0.009732133338161475 2119 3 -0.7008426263660752 2119 4 -0.08107779604613441 2119 5 0.2776750955710945 2119 6 0.45988692338352166 2119 7 0.33563976664970596 2119 8 0.5371896179677833 2119 9 0.31564565225810526 2119 10-11 0.4476975132694747 2119 12-13 0.3184729217071265 2119 14-15 0.5870771119408786 2119 16-17 0.3590433917457361 2119 18-19 0.12739496321412247 2119 20-21 -0.03664176462282143 2119 22-23 0.0620787836728951 2119 24-25 0.3425856774593754 2119 26-27 0.29085695487125207 2119 28-29 0.25879024433455555 2119 30-31 0.031245581264137456 2119 32-33 0.33906155700892526 2119 34-35 0.23499631599624848 2119 36-37 0.4609015532373135 2119 38-39 -0.06571647552279813 2119 40-41 0.10912242700762675 2119 42-43 0.05106254219715822 2119 44-45 0.1644404414361702 2119 46-47 -0.22041726071088164 2119 48-49 0.16637981312638317 2119 50-51 0.23827631575566244 2119 52-53 0.07792187508422899 2119 54-55 -0.01686507453236885 2119 56-57 -0.012890032260955309 2119 58-59 0.08127992086902225 2119 60-61 0.09181959468339329 2119 62-63 0.0528473463738095 2119 64-65 -0.13140664221656095 2119 66-67 -0.16689916549145778 2119 68-69 -0.5201007573439966 2119 70-71 0.06568604929647393 2119 72-73 0.20156269591917209 2119 74-75 0.311346110359878 2119 76 0.09128754539867145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 13.0 13 251.0 14 1393.0 15 2930.0 16 4132.0 17 4754.0 18 4849.0 19 4331.0 20 3575.0 21 2639.0 22 2114.0 23 1863.0 24 1777.0 25 1837.0 26 2042.0 27 2567.0 28 2936.0 29 3725.0 30 4591.0 31 5870.0 32 7456.0 33 10104.0 34 14472.0 35 21728.0 36 42547.0 37 161934.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.08798154410138 19.572733305944443 11.437600733179535 26.90168441677464 2 21.1301077647505 19.691874980248397 31.175615459975347 28.002401795025754 3 19.38185380652909 21.36523085674557 29.222892898903392 30.030022437821952 4 15.50611509654584 19.425781373447524 31.97484435736182 33.09325917264482 5 15.60029074360838 31.88351294125083 33.70002844230952 18.816167872831276 6 29.0715166071485 32.749739278829445 18.155674240748347 20.02306987327371 7 28.40036785503225 28.224341005407215 20.636540898590205 22.73875024097033 8 24.898397749897292 30.802389153999304 19.31801662294978 24.98119647315362 9 24.449641310874444 16.968681856966786 20.579275037133016 38.00240179502576 10-11 26.43301836109092 23.261858862939672 24.925891982428976 25.379230793540437 12-13 25.4787788768448 21.20990424422463 24.658060234491042 28.65325664443953 14-15 24.361350632605365 22.270205527613577 22.802077555980084 30.56636628380097 16-17 24.992731409790476 24.30553360932908 23.782037101412634 26.91969787946781 18-19 24.35530765098126 23.661789337294188 24.633094207249627 27.34980880447492 20-21 23.95932749739279 24.184969819549348 24.884334607970167 26.9713680750877 22-23 25.530607085295326 23.700660493632082 24.318174635780426 26.450557785292165 24-25 24.54855734285624 24.66548683753121 23.87242044053977 26.91353537907278 26-27 23.87905697942673 24.76898524160162 24.859842619220682 26.492115159750973 28-29 24.327339379957653 24.075466927914547 25.78232152450779 25.814872167620013 30-31 24.45975413203552 23.729892867300826 25.506905160699052 26.303447839964605 32-33 23.561135164175333 24.806118256802453 25.38460322978226 26.24814334923996 34-35 24.285623992668203 24.176121101033406 25.581013178270073 25.95724172802832 36-37 24.500837468002402 23.692443826438705 25.398192333217455 26.408526372341434 38-39 23.51404734064406 23.67032203014885 26.939291470467403 25.876339158739693 40-41 23.34623771450242 23.14714154789369 27.31362386625794 26.192996871345954 42-43 22.625383181114305 23.178270075530133 27.122270328350663 27.0740764150049 44-45 21.83911133584047 22.935404354833615 28.388901178775715 26.836583130550203 46-47 22.037452260298107 23.262685685121014 28.40932972431458 26.2905323302663 48-49 21.326833738899598 23.812533577726512 28.420503744904085 26.440128938469805 50-51 21.286382454255286 24.45611983693076 27.530891508390482 26.726606200423475 52-53 20.55137171371777 24.468206137869792 27.76120393516397 27.21921821324847 54-55 20.36693797512558 24.3324342389063 27.651024951845514 27.64960283412261 56-57 20.882659671965364 24.41740669342351 27.279019056347376 27.420914578263755 58-59 21.154915779161268 24.170432639130297 26.90926903264545 27.765382549062984 60-61 20.911734032803466 23.621338052649875 27.15908731789021 28.307840596656447 62-63 20.96119204879436 23.812059539234586 26.91353537907278 28.313213032898272 64-65 21.418955219163795 24.008627500553047 25.696994595961193 28.875422684321965 66-67 21.511866763581203 24.495149006099297 24.890339095534557 29.102645134784943 68-69 21.334768092102667 25.275140276112058 24.770920536802034 28.619171094983244 70-71 21.755206522769647 25.696362544638625 24.42799355307651 28.120437379515216 72-73 21.343583098947637 25.96956672881838 24.614922731725816 28.07192744050817 74-75 21.110580888603764 27.190617800517654 24.492698204020492 27.206103106858095 76 21.565659279016778 27.23106921299881 24.009493440866674 27.19377806711774 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 2.0 14 3.5 15 6.5 16 14.0 17 18.0 18 20.0 19 30.0 20 62.0 21 86.0 22 104.0 23 186.0 24 280.0 25 310.0 26 518.0 27 818.5 28 1082.0 29 1253.0 30 1543.5 31 2147.0 32 3138.0 33 3816.0 34 4452.5 35 5721.0 36 6908.5 37 7464.0 38 8481.0 39 10879.0 40 13408.5 41 15222.0 42 15887.0 43 16253.0 44 17456.0 45 18520.5 46 18748.0 47 18629.0 48 18037.0 49 16635.5 50 15707.0 51 14803.5 52 12451.5 53 10350.5 54 9698.0 55 9168.0 56 8186.0 57 7368.0 58 7002.0 59 6534.0 60 6047.0 61 5871.5 62 5715.0 63 5634.0 64 5542.5 65 5564.5 66 5645.0 67 5693.0 68 5518.5 69 5136.5 70 4868.0 71 4807.0 72 4595.0 73 3942.5 74 3084.5 75 2667.0 76 2279.5 77 1507.5 78 861.0 79 599.0 80 433.5 81 180.5 82 68.5 83 44.0 84 31.5 85 20.0 86 17.0 87 13.0 88 13.0 89 12.0 90 10.0 91 10.5 92 12.0 93 12.5 94 8.5 95 5.0 96 6.0 97 6.5 98 7.5 99 39.0 100 70.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 3.1602566128369626E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.5801283064184813E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.5801283064184813E-4 48-49 0.0 50-51 0.0 52-53 3.1602566128369626E-4 54-55 1.5801283064184813E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.5801283064184813E-4 70-71 0.0 72-73 0.0 74-75 3.1602566128369626E-4 76 3.1602566128369626E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 316430.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.74936675694257 #Duplication Level Percentage of deduplicated Percentage of total 1 68.90395567561065 32.90120250558847 2 14.819445644538026 14.152382904312425 3 6.509742600513158 9.325082607755874 4 2.8517131862502727 5.446699952634941 5 1.439476667873058 3.436704967616613 6 0.9144880286136714 2.6199734563864556 7 0.6557104438319801 2.1916830928223563 8 0.5084078544324315 1.9420922482723555 9 0.4188193017953116 1.7998520801679856 >10 2.9221252715241475 24.497520234073626 >50 0.05545514472295015 1.653076135999718 >100 6.601802943208352E-4 0.033729814369178074 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013273077773915242 0.0 2 0.0 0.0 0.0 0.0167493600480359 0.0 3 0.0 0.0 0.0 0.017381411370603294 0.0 4 0.0 0.0 0.0 0.05435641374079575 0.0 5 0.0 0.0 0.0 0.0584647473374838 0.0 6 0.0 0.0 0.0 0.05941282432133489 0.0 7 0.0 0.0 0.0 0.06004487564390228 0.0 8 0.0 0.0 0.0 0.061625003950320764 0.0 9 0.0 0.0 0.0 0.06636538886957621 0.0 10 0.0 0.0 0.0 0.07047372246626427 0.0 11 0.0 0.0 0.0 0.07047372246626427 0.0 12 0.0 0.0 0.0 0.07078974812754796 0.0 13 0.0 0.0 0.0 0.07205385077268274 0.0 14 0.0 0.0 0.0 0.07363397907910123 0.0 15 0.0 0.0 0.0 0.07489808172423601 0.0 16 0.0 0.0 0.0 0.07900641532092406 0.0 17 0.0 0.0 0.0 0.07995449230477515 0.0 18 0.0 0.0 0.0 0.08216667193376102 0.0 19 0.0 0.0 0.0 0.08753910817558386 0.0 20 0.0 0.0 0.0 0.08943526214328604 0.0 21 0.0 0.0 0.0 0.09164744177227191 0.0 22 0.0 0.0 0.0 0.09638782669152735 0.0 23 0.0 0.0 0.0 0.1008121859494991 0.0 24 0.0 0.0 0.0 0.10270833991720128 0.0 25 0.0 0.0 0.0 0.11345321240084695 0.0 26 0.0 0.0 0.0 0.12356603356192523 0.0 27 0.0 0.0 0.0 0.19909616660872864 0.0 28 0.0 0.0 0.0 0.28157886420377337 0.0 29 0.0 0.0 0.0 0.3700660493632083 0.0 30 0.0 0.0 0.0 0.4591852858452106 0.0 31 0.0 0.0 0.0 0.5716904212622065 0.0 32 0.0 0.0 0.0 0.6864077363081882 0.0 33 0.0 0.0 0.0 0.8456846695951711 0.0 34 0.0 0.0 0.0 1.0779635306386879 0.0 35 0.0 0.0 0.0 1.3873526530354265 0.0 36 0.0 0.0 0.0 1.8478020415257719 0.0 37 0.0 0.0 0.0 2.523148879689031 0.0 38 0.0 0.0 0.0 3.2184053345131627 0.0 39 0.0 0.0 0.0 3.9310432007078977 0.0 40 0.0 0.0 0.0 4.704041968207818 0.0 41 0.0 0.0 0.0 5.605031128527637 0.0 42 0.0 0.0 0.0 6.543627342540214 0.0 43 0.0 0.0 0.0 7.555857535631893 0.0 44 0.0 0.0 0.0 8.460006952564548 0.0 45 0.0 0.0 0.0 9.335082008659104 0.0 46 0.0 0.0 0.0 10.249028221091553 0.0 47 0.0 0.0 0.0 11.142748791201846 0.0 48 0.0 0.0 0.0 11.975160383023102 0.0 49 0.0 0.0 0.0 12.709604019846411 0.0 50 0.0 0.0 0.0 13.443415605347154 0.0 51 0.0 0.0 0.0 14.195556679202351 0.0 52 0.0 0.0 0.0 15.014695193249691 0.0 53 0.0 0.0 0.0 15.903359352779447 0.0 54 0.0 0.0 0.0 16.68267863350504 0.0 55 0.0 0.0 0.0 17.419334449957336 0.0 56 0.0 0.0 0.0 18.127863982555382 0.0 57 0.0 0.0 0.0 18.798786461460672 0.0 58 0.0 0.0 0.0 19.43557816894732 0.0 59 0.0 0.0 0.0 20.057516670353632 0.0 60 0.0 0.0 0.0 20.700628891065953 0.0 61 0.0 0.0 0.0 21.340264829504157 0.0 62 0.0 0.0 0.0 21.965363587523306 0.0 63 0.0 0.0 0.0 22.61542837278387 0.0 64 0.0 0.0 0.0 23.276870081850646 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTACGC 30 8.369367E-6 58.329514 20 GATTACG 25 2.3891532E-4 55.996334 19 TTAATAC 35 0.0012538192 40.0037 2 GTCTATA 35 0.0012538192 40.0037 1 GATATTA 35 0.0012538192 40.0037 1 TTAGATC 70 1.9217623E-8 39.99738 38 AGATCTA 50 1.7083458E-4 34.99771 40 TGTATAA 40 0.0024175697 34.997707 11 GCAATTT 40 0.0024175697 34.997707 13 GATCCTA 45 0.0043018395 31.11399 1 CGAAAGC 45 0.0043051685 31.109072 37 GGCAATT 45 0.0043051685 31.109072 12 ATCCGAC 45 0.0043051685 31.109072 10 AGCAAGC 70 3.480735E-5 30.002775 6 AGTAACC 70 3.480735E-5 30.002775 69 CATCCGA 60 4.978136E-4 29.164757 9 CGTGCGA 50 0.0072048996 27.998167 10 CCTGCAA 50 0.0072048996 27.998167 13 TAGATCT 265 0.0 27.734034 39 GCGACTC 65 7.942224E-4 26.92557 43 >>END_MODULE