FastQCFastQC Report
Wed 25 May 2016
SRR1780216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316430
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT137794.354517586828051No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC39721.2552539266188414No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG35421.119362892266852No Hit
CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG33001.0428846822361977RNA PCR Primer, Index 34 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC8860.27999873589735486No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT4560.14410770154536548RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT3520.11124103277186108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGTAC200.006947405652.560
TTAATAC506.182381E-849.0000043
TCTTTAT1700.047.352941
CTTTATA1750.046.02
TTTATAC2600.041.730773
TATACCG350.001254390240.06
ACGAGTG350.001254390240.05
GTGTTTC400.002416793535.016
GATAAAG400.002416793535.016
TGTATAA400.002416793535.014
AGTGCGG400.002416793535.08
CCGTCTT31400.032.5477747
CTCGTAT31700.032.46056739
TCGTATG32050.032.43369340
TATGCCG31950.032.42566343
CGTCTTC31750.032.29921348
CGTATGC32100.032.27414341
GCCGTCT32050.032.10608346
CTGCTTG32200.032.0652254
TCTCGTA31000.032.06451838