Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780216_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 316430 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT | 13779 | 4.354517586828051 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC | 3972 | 1.2552539266188414 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG | 3542 | 1.119362892266852 | No Hit |
CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG | 3300 | 1.0428846822361977 | RNA PCR Primer, Index 34 (96% over 25bp) |
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC | 886 | 0.27999873589735486 | No Hit |
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT | 456 | 0.14410770154536548 | RNA PCR Primer, Index 24 (95% over 23bp) |
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT | 352 | 0.11124103277186108 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTGGTAC | 20 | 0.0069474056 | 52.5 | 60 |
TTAATAC | 50 | 6.182381E-8 | 49.000004 | 3 |
TCTTTAT | 170 | 0.0 | 47.35294 | 1 |
CTTTATA | 175 | 0.0 | 46.0 | 2 |
TTTATAC | 260 | 0.0 | 41.73077 | 3 |
TATACCG | 35 | 0.0012543902 | 40.0 | 6 |
ACGAGTG | 35 | 0.0012543902 | 40.0 | 5 |
GTGTTTC | 40 | 0.0024167935 | 35.0 | 16 |
GATAAAG | 40 | 0.0024167935 | 35.0 | 16 |
TGTATAA | 40 | 0.0024167935 | 35.0 | 14 |
AGTGCGG | 40 | 0.0024167935 | 35.0 | 8 |
CCGTCTT | 3140 | 0.0 | 32.54777 | 47 |
CTCGTAT | 3170 | 0.0 | 32.460567 | 39 |
TCGTATG | 3205 | 0.0 | 32.433693 | 40 |
TATGCCG | 3195 | 0.0 | 32.425663 | 43 |
CGTCTTC | 3175 | 0.0 | 32.299213 | 48 |
CGTATGC | 3210 | 0.0 | 32.274143 | 41 |
GCCGTCT | 3205 | 0.0 | 32.106083 | 46 |
CTGCTTG | 3220 | 0.0 | 32.06522 | 54 |
TCTCGTA | 3100 | 0.0 | 32.064518 | 38 |