FastQCFastQC Report
Wed 25 May 2016
SRR1780215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712006
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA250.002345329635.2208240
GTCTTAA250.002355058235.19111611
CAATCGA401.8116798E-533.0195238
AATCGAG454.0508407E-529.35068339
ATACCGC801.3212775E-724.724647
CAACGGT450.001393412624.45718213
TATACCG752.0807474E-623.4426236
CGAATCA852.3364555E-723.30789635
GAGTTAG500.002555895822.03471220
CCGAATC904.0537634E-722.01301234
CGATAAC500.002591631421.9820943
CTATAAC500.002594804221.9774594
TGACAAT755.4437332E-520.54547935
ATCTTTT654.9098005E-420.31970434
CGAGTAG654.954222E-420.29258542
CGGTATG654.956569E-420.29115943
AACGGTC550.00446106320.01605414
GACGATA550.004501173719.98512341
GTCATAC1101.2775308E-719.9795071
TCAGATG550.004508498619.9795074
Produced by FastQC (version 0.11.5)