FastQCFastQC Report
Wed 25 May 2016
SRR1780215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712006
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT191882.6949211102153634No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC58580.8227458757370023No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG47480.6668483130760133No Hit
CACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTTCTG37770.5304730578113106TruSeq Adapter, Index 2 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC10890.1529481493133485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA1850.036.8658642
TCTTCTG5500.034.798544
GTCTTCT5750.034.433343
CCGTCTT5700.033.9634541
CGTCTTC5800.033.37787242
ATGCCGT26450.031.35592844
CGAATAT454.0684183E-529.33207125
ATTTCCG300.00574465829.3320725
GCCGTCT6700.028.89427640
TATGCCG30250.027.41700243
TTTATAC3050.027.4105823
TTTTCGA508.339148E-526.39886321
TCTTTAT2600.026.2739221
TGCCGTC8150.024.02350639
GAATATC551.593187E-423.99896426
TCTAGCA651.8116529E-523.6929513
CGGCTTA651.812638E-523.69128625
ATACAAT1053.0668161E-923.0466276
ACAACTT500.002580051821.99905223
AAGGCAG500.002580051821.9990525
Produced by FastQC (version 0.11.5)