FastQCFastQC Report
Wed 25 May 2016
SRR1780214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135665
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATATA150.002220665370.01
ACGGAAA150.002220665370.031
ACGTTGA200.00693889452.49999654
ATTACGG200.00693889452.49999626
CGACAGG200.00693889452.4999961
AACGGAA200.00693889452.49999630
AATCGAG200.00693889452.49999662
CTTATAA501.7025576E-435.0000041
TACGTAC400.002411944834.99999623
AATGATT552.979655E-431.8181825
CTGCTAT450.00429520731.11110922
CCCTCAG450.00429520731.11110919
CTGAGAC450.00429520731.1111093
GCGCGAG500.00718832128.00000241
TATGGCT500.00718832128.0000024
GCAGTCC500.00718832128.00000228
ACTACGT500.00718832128.00000221
TGGCTTC500.00718832128.00000222
GGTAGAA500.00718832128.00000215
GTTGCCG500.00718832128.00000218
Produced by FastQC (version 0.11.5)