##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780214_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 135665 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.384609147532526 34.0 27.0 34.0 12.0 34.0 2 29.601511075074633 34.0 31.0 34.0 12.0 34.0 3 29.413061585523163 34.0 27.0 34.0 11.0 34.0 4 29.289278738068035 34.0 27.0 34.0 11.0 34.0 5 29.330660081819186 34.0 27.0 34.0 11.0 34.0 6 32.00317694320569 37.0 31.0 38.0 11.0 38.0 7 32.31100873475104 37.0 31.0 38.0 12.0 38.0 8 32.398223565400066 38.0 31.0 38.0 11.0 38.0 9 32.580496074890355 38.0 32.0 38.0 12.0 38.0 10-11 32.273077064828804 38.0 31.0 38.0 11.0 38.0 12-13 32.33888254155457 38.0 31.0 38.0 11.0 38.0 14-15 32.19806140124572 38.0 31.0 38.0 11.0 38.0 16-17 32.23983709873586 38.0 31.0 38.0 11.0 38.0 18-19 32.249566947996904 38.0 31.0 38.0 11.0 38.0 20-21 32.28005749456382 38.0 31.0 38.0 11.0 38.0 22-23 32.29143846976007 38.0 31.0 38.0 11.0 38.0 24-25 32.249441639332176 38.0 31.0 38.0 11.0 38.0 26-27 32.18782294622784 38.0 31.0 38.0 11.0 38.0 28-29 32.23131242398556 38.0 31.0 38.0 11.0 38.0 30-31 32.253753731618325 38.0 31.0 38.0 11.0 38.0 32-33 32.334024988021966 38.0 31.0 38.0 11.0 38.0 34-35 32.28259315225003 38.0 31.0 38.0 11.0 38.0 36-37 32.22144252386393 38.0 31.0 38.0 11.0 38.0 38-39 32.188700106880916 38.0 31.0 38.0 11.0 38.0 40-41 32.26677846165186 38.0 31.0 38.0 11.0 38.0 42-43 32.33523753363063 38.0 31.0 38.0 11.0 38.0 44-45 32.43732723989238 38.0 31.0 38.0 11.0 38.0 46-47 32.3309438690893 38.0 31.5 38.0 11.0 38.0 48-49 32.261463900047914 38.0 31.0 38.0 11.0 38.0 50-51 32.1802454575609 38.0 31.0 38.0 11.0 38.0 52-53 32.29340655290605 38.0 31.0 38.0 11.0 38.0 54-55 32.26791729628128 38.0 31.0 38.0 11.0 38.0 56-57 32.22551505546751 38.0 31.0 38.0 11.0 38.0 58-59 32.25157188663252 38.0 31.0 38.0 11.0 38.0 60-61 32.29172225703019 38.0 31.0 38.0 11.0 38.0 62-63 32.16969004533225 38.0 31.0 38.0 11.0 38.0 64-65 32.1200493863561 38.0 31.0 38.0 11.0 38.0 66-67 32.088972837504144 38.0 30.5 38.0 11.0 38.0 68-69 32.026031032322265 38.0 30.5 38.0 11.0 38.0 70-71 32.01561567095419 37.5 30.5 38.0 11.0 38.0 72-73 32.02952493273873 38.0 30.5 38.0 11.0 38.0 74-75 32.02866619983047 38.0 30.0 38.0 11.0 38.0 76 30.566940625806215 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5940031173045668 1101 2 -0.6241215050760829 1101 3 -0.15119236386451718 1101 4 0.0969980781364228 1101 5 -0.5736461085398865 1101 6 -0.3207071455877788 1101 7 -0.41069798095914933 1101 8 -0.5999545855530286 1101 9 -0.7042787933844572 1101 10-11 -0.8523174737294212 1101 12-13 -0.5885215157270061 1101 14-15 -0.3558678771104482 1101 16-17 -0.5845193876965169 1101 18-19 -0.10851078966145877 1101 20-21 -0.10322202700363903 1101 22-23 -0.4211865678755977 1101 24-25 -0.4922319845816432 1101 26-27 -0.6026055590167942 1101 28-29 -0.550435979835008 1101 30-31 -0.6626159173815296 1101 32-33 -0.5977036410857508 1101 34-35 -0.6766931698072902 1101 36-37 -0.8264721730310747 1101 38-39 -0.603684762810289 1101 40-41 -0.5327823183695983 1101 42-43 -0.712189430726859 1101 44-45 -0.7489689381714406 1101 46-47 -0.6078650876225922 1101 48-49 -0.39675868522932944 1101 50-51 -0.6473601288278985 1101 52-53 -0.3499725801349314 1101 54-55 -0.5936971756037615 1101 56-57 -0.39695052971151057 1101 58-59 -0.3597963519521663 1101 60-61 -0.7834616373566377 1101 62-63 -0.7685425668269552 1101 64-65 -0.7909980511876356 1101 66-67 -0.7901669212120837 1101 68-69 -0.7327253020593147 1101 70-71 -0.5459181997563292 1101 72-73 -0.8941062261706065 1101 74-75 -0.5356254940910574 1101 76 -1.4995576640153416 1102 1 0.25514257863778766 1102 2 -0.2303480444502526 1102 3 -0.12329753095575668 1102 4 0.46643200745893054 1102 5 0.12717684855048006 1102 6 -0.24137154257529403 1102 7 -0.813835852063594 1102 8 -0.21054589252316447 1102 9 -0.369889979887013 1102 10-11 0.1808547599999173 1102 12-13 0.40907786209521646 1102 14-15 0.14979838482322805 1102 16-17 0.03672988814885514 1102 18-19 -0.083679230010965 1102 20-21 0.16318751517191998 1102 22-23 -0.32141194860237476 1102 24-25 -0.11915545830177621 1102 26-27 -0.02535314053874771 1102 28-29 -0.23293484896340289 1102 30-31 0.007320357011572298 1102 32-33 0.08368022832040367 1102 34-35 -0.02897753044820206 1102 36-37 0.29134084032511254 1102 38-39 -0.06501688988057452 1102 40-41 0.2526179308189498 1102 42-43 -0.027127252628467602 1102 44-45 0.24272213151405708 1102 46-47 -0.47652129471891413 1102 48-49 0.2004843688382394 1102 50-51 -0.10852956910597555 1102 52-53 -0.05439448650670187 1102 54-55 -0.05364051298442263 1102 56-57 -0.09085334617699914 1102 58-59 -0.05291351025038438 1102 60-61 0.11453771189619744 1102 62-63 0.12541738841433414 1102 64-65 0.3598046692529664 1102 66-67 0.3358240794307292 1102 68-69 0.41466099574481063 1102 70-71 0.39475667267342374 1102 72-73 0.4044994289810049 1102 74-75 -0.048799156289788925 1102 76 0.17959223797578616 1103 1 0.6973254810662866 1103 2 0.579209019381203 1103 3 0.2502361502400525 1103 4 0.9964724170473822 1103 5 0.5863474711720045 1103 6 -0.12425435476878732 1103 7 0.7389830109753603 1103 8 1.0772253028944618 1103 9 0.9408558320318576 1103 10-11 0.24332876839569906 1103 12-13 0.6898421133408448 1103 14-15 0.2864337961771426 1103 16-17 0.7704503165689616 1103 18-19 0.3533406609223917 1103 20-21 0.6906385724907622 1103 22-23 0.06376989454565063 1103 24-25 -0.03457779734999633 1103 26-27 0.3751882694812565 1103 28-29 0.6974272680282425 1103 30-31 0.24373214248765862 1103 32-33 0.44189749851538807 1103 34-35 0.28884192107991424 1103 36-37 1.019717787243394 1103 38-39 0.875726175755581 1103 40-41 0.8706806245299603 1103 42-43 0.4877222903116234 1103 44-45 0.7214776731202406 1103 46-47 0.6874200132963111 1103 48-49 0.774241675911874 1103 50-51 0.1976260274258621 1103 52-53 0.5875098643897658 1103 54-55 0.6638807707065837 1103 56-57 0.7338788912587617 1103 58-59 0.4700218736407038 1103 60-61 0.5945408505050054 1103 62-63 0.26375549504857076 1103 64-65 1.06201346520416 1103 66-67 0.7256510636199849 1103 68-69 0.8136291780979832 1103 70-71 0.5233807850356129 1103 72-73 0.727301421997602 1103 74-75 0.6844740203746511 1103 76 0.524125173752001 1104 1 0.3545472430105008 1104 2 0.15815721837911312 1104 3 0.3775490472298344 1104 4 0.5773064407977273 1104 5 0.1495936146998389 1104 6 0.6123771992612745 1104 7 0.44141722409722917 1104 8 0.04317788110884635 1104 9 -0.16916902298056868 1104 10-11 -0.05839135627388714 1104 12-13 0.18452787496944723 1104 14-15 -0.0028548770386223055 1104 16-17 0.09341161376389806 1104 18-19 0.03136567374441768 1104 20-21 0.11871290081364094 1104 22-23 0.3932092759320156 1104 24-25 0.1665939389501574 1104 26-27 0.05474798068969022 1104 28-29 0.24784743609546922 1104 30-31 0.503715572479372 1104 32-33 0.117527750616226 1104 34-35 0.1381455862079335 1104 36-37 0.1072705548292987 1104 38-39 0.10159906012059139 1104 40-41 0.23218691718786744 1104 42-43 0.4235332344075715 1104 44-45 0.3561089904358994 1104 46-47 0.20372963573683478 1104 48-49 0.13211440246728756 1104 50-51 0.5913096980772252 1104 52-53 0.3733030549054135 1104 54-55 0.30801222610564594 1104 56-57 0.4074378923239763 1104 58-59 0.53972242991955 1104 60-61 0.5016541183473606 1104 62-63 0.1808936179033509 1104 64-65 0.4470373339065645 1104 66-67 0.5611582242307094 1104 68-69 0.4610667446739072 1104 70-71 0.17649555206918421 1104 72-73 0.6203183470774931 1104 74-75 0.6687246220237597 1104 76 0.688428641618021 1105 1 0.23363221033076087 1105 2 0.03521915426457767 1105 3 0.10521789254573832 1105 4 -0.03100354986520415 1105 5 -0.09643813133190804 1105 6 0.4147425898619588 1105 7 0.5097334394722708 1105 8 -0.16161514721358827 1105 9 0.32787423876857247 1105 10-11 0.10718598577403782 1105 12-13 -1.1172150444205329 1105 14-15 0.23835271711014627 1105 16-17 0.07512652194939307 1105 18-19 0.6440329628822994 1105 20-21 0.6614299876483756 1105 22-23 0.3832497365607068 1105 24-25 0.22378348143382 1105 26-27 0.2149565085452707 1105 28-29 0.2476918182927932 1105 30-31 0.43781143304582315 1105 32-33 -0.03614482952693976 1105 34-35 0.31282656971244904 1105 36-37 0.14920955265064606 1105 38-39 -0.05596396508948942 1105 40-41 -0.019758305345590088 1105 42-43 0.40034618180875015 1105 44-45 0.2531678139653124 1105 46-47 -0.03491026466777214 1105 48-49 0.035475747005101255 1105 50-51 -0.1171403486081104 1105 52-53 0.3357213555590093 1105 54-55 0.5844672525606711 1105 56-57 0.4773880946271163 1105 58-59 0.6615711694153532 1105 60-61 0.22193111803612453 1105 62-63 0.48746068917630225 1105 64-65 0.11946185927227759 1105 66-67 0.02505489400972749 1105 68-69 0.18017661084259373 1105 70-71 0.11494871111058558 1105 72-73 0.14801831595394077 1105 74-75 0.15892070045513407 1105 76 -0.020721685179363902 1106 1 0.11518357301419968 1106 2 0.491543403041657 1106 3 0.8906826025387495 1106 4 0.5001435001497683 1106 5 0.6985603712137625 1106 6 -0.04118233776108582 1106 7 -0.23095738612421712 1106 8 0.18978746833996496 1106 9 0.5440068133917109 1106 10-11 -0.25993990021977 1106 12-13 0.48046889288604433 1106 14-15 0.2292681608557814 1106 16-17 0.3738936178863028 1106 18-19 0.2817488458811894 1106 20-21 0.1989412185558308 1106 22-23 -0.003942037423520617 1106 24-25 -0.21369780038708086 1106 26-27 0.004089981641016038 1106 28-29 0.1367803677586963 1106 30-31 0.15077439600878506 1106 32-33 -0.05648525081075917 1106 34-35 0.19614992001693565 1106 36-37 0.2381832972847775 1106 38-39 0.2600737707595613 1106 40-41 0.49187192326576934 1106 42-43 0.3669030351580602 1106 44-45 0.3643537977928091 1106 46-47 0.3358843844287662 1106 48-49 -0.059762348232993645 1106 50-51 -0.18145601198038008 1106 52-53 0.28623049003040535 1106 54-55 -0.13358705511627988 1106 56-57 -0.032470146363202446 1106 58-59 -0.0028493157598461494 1106 60-61 -0.29032304987842394 1106 62-63 -0.0493267721394659 1106 64-65 0.19982524152244707 1106 66-67 0.05235847791708892 1106 68-69 0.11708386850457231 1106 70-71 0.21690039325093835 1106 72-73 0.3026554328552038 1106 74-75 -0.014933746984219454 1106 76 0.7593022740898796 1107 1 0.9305880703003204 1107 2 0.6844762391380996 1107 3 0.8262731944229564 1107 4 0.017811409908659925 1107 5 0.20112655664373946 1107 6 0.02886160124124615 1107 7 -0.042263371428646224 1107 8 0.12167832238124987 1107 9 -0.0876218835768654 1107 10-11 0.1403961039156627 1107 12-13 0.38440124897658734 1107 14-15 0.2799124764781311 1107 16-17 0.11561720833048383 1107 18-19 0.3664218880109402 1107 20-21 0.18546782196018086 1107 22-23 0.5132200199556465 1107 24-25 0.5697826116795284 1107 26-27 0.26152822703480183 1107 28-29 1.0357834324118045 1107 30-31 0.3781716562827597 1107 32-33 0.5323084574194965 1107 34-35 0.47653244780188686 1107 36-37 0.36158543146488853 1107 38-39 0.19951548696530352 1107 40-41 0.4043483845145275 1107 42-43 0.42175663335618907 1107 44-45 0.2675513756091519 1107 46-47 0.29731775591641707 1107 48-49 0.5476740509842273 1107 50-51 0.3338598688304586 1107 52-53 0.3289916317059962 1107 54-55 0.07011268272664495 1107 56-57 0.44155784167357837 1107 58-59 -0.07678499496820734 1107 60-61 0.4800125850216972 1107 62-63 0.5598967259958982 1107 64-65 0.3791849874885571 1107 66-67 0.2568299175655824 1107 68-69 0.19082385299668658 1107 70-71 0.2516269745011783 1107 72-73 0.6328773432787145 1107 74-75 0.45539819556276484 1107 76 0.6728421330419891 1108 1 -0.6902706841603674 1108 2 -0.6457643502483386 1108 3 -0.25270305361050305 1108 4 -0.136979273487988 1108 5 -0.41623271583237553 1108 6 -0.4296396074614144 1108 7 -0.32048753780753003 1108 8 0.13001261500240702 1108 9 -0.6985807876864527 1108 10-11 -0.09760704254840391 1108 12-13 -0.35888949197733666 1108 14-15 -0.19893330841117063 1108 16-17 -0.26233068315487174 1108 18-19 -0.2501469312975999 1108 20-21 -0.24263163700148027 1108 22-23 -0.5409643935357771 1108 24-25 -0.4552548005456387 1108 26-27 -0.166540534716475 1108 28-29 -0.01265676558519857 1108 30-31 -0.41785305497160863 1108 32-33 -0.1919960589075842 1108 34-35 -0.021518279338280877 1108 36-37 -0.6252224423695765 1108 38-39 -0.723330911868203 1108 40-41 -0.39106238253201653 1108 42-43 -0.5610606031274443 1108 44-45 -0.27554367062852947 1108 46-47 -0.11839921580377677 1108 48-49 -0.20962229221058237 1108 50-51 -0.20420850920569222 1108 52-53 -0.6491059086800206 1108 54-55 -0.4174779699727864 1108 56-57 -0.39648367630347536 1108 58-59 -0.44347084739136733 1108 60-61 -0.20983047549017897 1108 62-63 -0.4535601636092572 1108 64-65 -0.3092651871018326 1108 66-67 -0.4276372939765736 1108 68-69 -0.43109011807118947 1108 70-71 -0.48036719302884734 1108 72-73 -0.48752479017740313 1108 74-75 -0.28645745080536855 1108 76 -0.8858010502587277 1109 1 -0.1172372267455728 1109 2 -0.0692536398633905 1109 3 0.13606571649701138 1109 4 0.23767987399063273 1109 5 -0.013473203539394518 1109 6 -0.055946363585619 1109 7 -0.1218680369567906 1109 8 0.043527092411409285 1109 9 0.0335147030297307 1109 10-11 0.3007427482963223 1109 12-13 0.08408876033155366 1109 14-15 0.0966814892320258 1109 16-17 -0.1691544206074127 1109 18-19 0.42125637803673754 1109 20-21 0.3074764699017578 1109 22-23 0.12002573195739075 1109 24-25 -0.151284936738044 1109 26-27 0.13548266183005353 1109 28-29 -0.6474003181786543 1109 30-31 0.12159667717588718 1109 32-33 0.5166985595233413 1109 34-35 0.3729678970951511 1109 36-37 -0.08155616949437672 1109 38-39 0.29217927098478214 1109 40-41 -0.02153366918991395 1109 42-43 0.2368723631625258 1109 44-45 0.046934392214645015 1109 46-47 0.1623480963836954 1109 48-49 0.4688469734797778 1109 50-51 0.48276070818949535 1109 52-53 0.7143264669227349 1109 54-55 0.33232699179971803 1109 56-57 0.20620302171790428 1109 58-59 0.5448602940837901 1109 60-61 0.658821494581673 1109 62-63 0.5398865519469922 1109 64-65 0.691957052457127 1109 66-67 0.7482946172494565 1109 68-69 0.6557706209193626 1109 70-71 0.7217407058336107 1109 72-73 -0.03562307458400937 1109 74-75 0.43554063224748063 1109 76 0.37086182709380466 1110 1 0.09610944166045243 1110 2 -0.034821612497502485 1110 3 -0.2839745786139467 1110 4 -0.5377690976143583 1110 5 0.02469119471545156 1110 6 0.24152721992527404 1110 7 -0.4338869959514433 1110 8 0.08719220815113715 1110 9 0.6074301412752945 1110 10-11 0.30841766241555035 1110 12-13 0.2699390886352404 1110 14-15 0.6333783047586863 1110 16-17 0.37532601887019723 1110 18-19 0.6007508048460366 1110 20-21 0.6702121890535295 1110 22-23 0.3495737889503303 1110 24-25 0.6532656568176378 1110 26-27 0.7585639333002376 1110 28-29 0.5690119674817922 1110 30-31 0.05924434136397849 1110 32-33 0.41894667884326253 1110 34-35 0.492005162825464 1110 36-37 0.3705643969563184 1110 38-39 0.39895406769739594 1110 40-41 0.6181330989392606 1110 42-43 0.5299758114383835 1110 44-45 0.2231131691565693 1110 46-47 0.3664471817716475 1110 48-49 0.7064359343587299 1110 50-51 0.3417459381470209 1110 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20-21 0.3454959217143099 2116 22-23 0.4833718366828066 2116 24-25 -0.2002091925588516 2116 26-27 0.16662521021397936 2116 28-29 0.3128118834128557 2116 30-31 0.48125872649311674 2116 32-33 0.6116980683159596 2116 34-35 0.4087769156627985 2116 36-37 0.267544920986019 2116 38-39 0.3008736417481117 2116 40-41 0.16094987536259708 2116 42-43 0.2792635607261289 2116 44-45 0.5031678139653124 2116 46-47 0.6043856246281223 2116 48-49 0.6231843347136916 2116 50-51 0.7970843156165515 2116 52-53 0.312420582258234 2116 54-55 0.0473284654218844 2116 56-57 0.6187189859580045 2116 58-59 0.38379339163757464 2116 60-61 0.3760825221875237 2116 62-63 0.6944203961360103 2116 64-65 0.34005607986649267 2116 66-67 0.36652823548307367 2116 68-69 0.7444826751486602 2116 70-71 0.3472441934060697 2116 72-73 0.2668624347980497 2116 74-75 0.35896140049583636 2116 76 0.7027215382638587 2117 1 -0.3755956616089762 2117 2 -0.39492164028299825 2117 3 0.22700069229463793 2117 4 -0.8783266599001784 2117 5 0.09471064456262823 2117 6 0.09611645767650145 2117 7 0.27038270859611657 2117 8 0.20315034467054716 2117 9 -0.04321355604803756 2117 10-11 0.2535181532926458 2117 12-13 -0.03276073538995661 2117 14-15 0.35320788705006123 2117 16-17 0.21141474722066533 2117 18-19 0.28617268129320195 2117 20-21 0.06377058490422627 2117 22-23 -0.15248696866752454 2117 24-25 0.11610255595628871 2117 26-27 0.2189696015414171 2117 28-29 -0.38308120654802735 2117 30-31 0.243994734991837 2117 32-33 0.0955698148995694 2117 34-35 0.20875518598005272 2117 36-37 0.28206759228835665 2117 38-39 0.5245445094867804 2117 40-41 0.17074030464454637 2117 42-43 -0.21578690042601778 2117 44-45 -0.3005045024188675 2117 46-47 0.2681791443538444 2117 48-49 -0.11383821586817788 2117 50-51 0.16648892230929135 2117 52-53 -0.05659771896345944 2117 54-55 0.35885968373276 2117 56-57 0.0022961746199428035 2117 58-59 0.08671241235320792 2117 60-61 -0.20184253455109058 2117 62-63 -0.28980143978074224 2117 64-65 -0.18862710135906013 2117 66-67 0.06141934562842266 2117 68-69 0.014003173482720399 2117 70-71 0.3176435366867665 2117 72-73 -0.9849966602813822 2117 74-75 -0.3132342632252545 2117 76 -0.6505306709205527 2118 1 0.4799814166799692 2118 2 0.25574825479367647 2118 3 0.15844540577325006 2118 4 0.058202799341145806 2118 5 -0.1685016233954002 2118 6 0.6959066110259826 2118 7 0.9634900532288881 2118 8 0.7111890855906395 2118 9 0.3671334980278331 2118 10-11 -0.2094277708397172 2118 12-13 0.2141077069022117 2118 14-15 0.3141199128773451 2118 16-17 0.1890418658647306 2118 18-19 0.17937687822620774 2118 20-21 0.2547633209817093 2118 22-23 0.22584232915329494 2118 24-25 0.29600570365604284 2118 26-27 0.24088243447120306 2118 28-29 0.3045701251711037 2118 30-31 -0.003511318276927966 2118 32-33 0.1590403556582487 2118 34-35 0.12668900357488155 2118 36-37 -0.030684627243530826 2118 38-39 0.10260746348193805 2118 40-41 0.3481252925380076 2118 42-43 0.4999758114383823 2118 44-45 0.4560249568224535 2118 46-47 0.15011619035868762 2118 48-49 0.3916662231955783 2118 50-51 -0.09126733273510013 2118 52-53 0.23730865714630767 2118 54-55 -0.0315115834181654 2118 56-57 -0.2886965614575381 2118 58-59 -0.0898574020132159 2118 60-61 -0.06859798249298166 2118 62-63 -0.3528038610882476 2118 64-65 0.05020260001300869 2118 66-67 -0.0777493387945043 2118 68-69 -0.30590804524206305 2118 70-71 0.057223843385713735 2118 72-73 -0.2706060226704068 2118 74-75 -0.30679358525915035 2118 76 0.24086561640793747 2119 1 -0.09401169863845027 2119 2 -0.2954049293715535 2119 3 -0.6479057129875052 2119 4 -0.21923265676900883 2119 5 -0.19349301754342108 2119 6 -0.746967743535123 2119 7 -0.7560280190602704 2119 8 -0.7773479195728719 2119 9 -0.21665307684308033 2119 10-11 -0.08509902729410612 2119 12-13 -0.29037594197902195 2119 14-15 -0.11213654151163865 2119 16-17 -0.11710271244852066 2119 18-19 -0.4188933455259445 2119 20-21 -0.5773106893453175 2119 22-23 -0.46864992557992124 2119 24-25 -0.601326482230359 2119 26-27 -0.14117436415186546 2119 28-29 -0.1835968283573024 2119 30-31 -0.8567557244731425 2119 32-33 -0.7653314171472623 2119 34-35 -0.48695987766436133 2119 36-37 -0.6171734396359554 2119 38-39 -0.6071687843665998 2119 40-41 -0.7630967384430605 2119 42-43 -0.6597832247061959 2119 44-45 -0.662494836637098 2119 46-47 -0.4645653931180789 2119 48-49 -0.6572236218990852 2119 50-51 -0.7482122552824322 2119 52-53 -0.9390682791703853 2119 54-55 -0.5770279345369289 2119 56-57 -0.5012008643835273 2119 58-59 -0.6833083486569365 2119 60-61 -0.7914637312021 2119 62-63 -0.7816076476631224 2119 64-65 -1.0982741641866411 2119 66-67 -0.6845135384502683 2119 68-69 -0.7076206097859483 2119 70-71 -0.8745489621220308 2119 72-73 -0.6101413066635182 2119 74-75 -0.7246851515242128 2119 76 -0.18686243866951457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 28.0 13 414.0 14 1580.0 15 2797.0 16 3695.0 17 4469.0 18 4580.0 19 4059.0 20 3172.0 21 2197.0 22 1677.0 23 1211.0 24 1076.0 25 1037.0 26 970.0 27 1164.0 28 1332.0 29 1495.0 30 1921.0 31 2359.0 32 3018.0 33 3933.0 34 5396.0 35 8325.0 36 15649.0 37 58109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.8613865035197 18.98647403530756 14.79821619430214 29.353923266870602 2 19.189179228246047 18.209560314008773 34.86897873438249 27.732281723362696 3 18.743964913573876 19.84594405336675 27.018759444219214 34.39133158884016 4 15.5485939630708 20.792393026941365 27.286330298898022 36.372682711089816 5 16.25474514428924 32.975343677440755 29.45269597906608 21.317215199203922 6 32.06869863266134 29.36571702355066 15.994545387535473 22.571038956252533 7 28.386098109313384 24.267865698595806 20.75480042752368 26.591235764567134 8 24.165407437437807 32.055430656396275 18.127004017248368 25.652157888917554 9 23.38701949655401 17.339033649062028 18.448383886779936 40.82556296760402 10-11 26.912615634098696 21.898426270593006 23.055320089927395 28.133638005380902 12-13 25.335200678141007 19.029594958169017 23.437142962444256 32.19806140124572 14-15 24.834334574134818 20.231083919949878 21.156893819334393 33.77768768658092 16-17 26.533741200751855 21.766115062838608 21.364758780820402 30.335384955589134 18-19 25.673534072900157 20.562414771680242 22.107396896767774 31.656654258651827 20-21 25.281391663288243 21.132937751078025 22.692662072015626 30.893008513618103 22-23 27.067777245420704 20.90922492905318 21.317952308996425 30.70504551652969 24-25 25.829064239118416 22.182950650499393 20.59632182213541 31.391663288246786 26-27 24.85165665425865 22.39597538053293 22.600154793056426 30.152213172151992 28-29 25.613091069914862 21.665868131058122 23.590461799285002 29.130578999742013 30-31 25.19588692735783 20.99288689050234 23.869826410643867 29.941399771495963 32-33 24.52732834555707 22.138355508052925 23.685917517414218 29.648398628975787 34-35 24.68027862750157 21.961449157852062 23.817123060479858 29.54114915416651 36-37 24.96627722699296 20.19091143625843 24.409390778756496 30.433420557992115 38-39 23.606309659823832 20.33575351048539 26.901558987211143 29.156377842479635 40-41 23.53259868057347 20.03243283087016 26.949471123723878 29.48549736483249 42-43 21.767589282423614 20.10909224929053 27.055246378948144 31.068072089337704 44-45 20.842147937935355 20.067076991117826 28.963255076843698 30.127519994103118 46-47 21.19559208344083 21.396454501898056 28.44064423395865 28.967309180702465 48-49 20.918438801459477 21.942652858143223 27.74997235838278 29.38893598201452 50-51 20.633177311760587 22.88799616702908 26.40732687133748 30.071499649872845 52-53 19.839678620130467 23.03431246084104 26.946522684553862 30.179486234474624 54-55 19.95135075369476 22.170419784026834 26.670106512365017 31.208122949913392 56-57 20.48980945711864 22.595363579405152 25.984225850440424 30.930601113035788 58-59 20.821140308849003 22.567353407290017 25.60866841115984 31.00283787270114 60-61 20.57826263221907 21.699406626617034 25.78704898094571 31.93528176021819 62-63 20.504920207864963 22.02520915490362 25.425496627722698 32.044374009508715 64-65 20.819666089263997 22.597206353886413 24.36516419120628 32.217963365643314 66-67 20.773965282128774 24.162827553164043 22.800648656617405 32.262558508089775 68-69 20.68551210702834 25.334095013452252 22.37128220248406 31.609110677035346 70-71 20.56020344230273 26.597132642907162 22.063170309217558 30.77949360557255 72-73 20.181697563852136 26.491357387682896 22.947702060221868 30.3792429882431 74-75 20.32395975380533 27.82073489846313 23.09364979913758 28.76165554859396 76 20.788707477978846 27.702060221870045 22.722883573508273 28.786348726642835 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 2.0 14 2.5 15 2.5 16 5.0 17 7.0 18 13.5 19 19.0 20 27.5 21 37.0 22 55.0 23 93.0 24 128.5 25 144.0 26 201.0 27 333.0 28 485.0 29 562.0 30 652.5 31 909.5 32 1280.0 33 1484.0 34 1691.0 35 2140.5 36 2537.0 37 2691.0 38 3040.0 39 3700.0 40 4369.0 41 5002.0 42 5277.0 43 5374.0 44 5677.0 45 5887.5 46 5892.0 47 5904.0 48 5625.0 49 5121.5 50 4909.0 51 4558.5 52 3925.5 53 3567.0 54 3491.0 55 3504.0 56 3583.0 57 3773.5 58 3898.0 59 3906.5 60 4112.0 61 4351.5 62 4394.0 63 4392.5 64 4413.5 65 4499.0 66 4439.5 67 4317.0 68 4342.5 69 4156.5 70 3681.0 71 3417.0 72 3196.5 73 2663.5 74 1920.5 75 1490.0 76 1193.5 77 647.0 78 275.5 79 154.0 80 101.0 81 31.5 82 16.0 83 17.0 84 14.0 85 9.0 86 7.5 87 8.0 88 8.5 89 9.0 90 9.5 91 8.0 92 6.0 93 6.0 94 5.5 95 5.0 96 5.0 97 6.0 98 7.5 99 22.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 135665.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.46813842921904 #Duplication Level Percentage of deduplicated Percentage of total 1 75.65928094572715 50.28931559355766 2 13.625878856433118 18.113736040983305 3 5.497149954532349 10.961559724320939 4 2.206844544990796 5.867393948328604 5 1.0513008184177257 3.493900416467033 6 0.601060172555282 2.3970810452216855 7 0.3748308825160246 1.744001769063502 8 0.28278661254907184 1.5037039767073306 9 0.18630647414997673 1.1145100062654334 >10 0.5145607381285071 4.51479747908451 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.371097925035935E-4 0.0 2 0.0 0.0 0.0 7.371097925035935E-4 0.0 3 0.0 0.0 0.0 0.00442265875502156 0.0 4 0.0 0.0 0.0 0.022113293775107803 0.0 5 0.0 0.0 0.0 0.02579884273762577 0.0 6 0.0 0.0 0.0 0.02579884273762577 0.0 7 0.0 0.0 0.0 0.026535952530129362 0.0 8 0.0 0.0 0.0 0.026535952530129362 0.0 9 0.0 0.0 0.0 0.03538127004017248 0.0 10 0.0 0.0 0.0 0.04201525817270482 0.0 11 0.0 0.0 0.0 0.046437916927726385 0.0 12 0.0 0.0 0.0 0.04864924630523716 0.0 13 0.0 0.0 0.0 0.04864924630523716 0.0 14 0.0 0.0 0.0 0.05380901485276232 0.0 15 0.0 0.0 0.0 0.05528323443776951 0.0 16 0.0 0.0 0.0 0.057494563815280286 0.0 17 0.0 0.0 0.0 0.06265433236280545 0.0 18 0.0 0.0 0.0 0.07149964987284856 0.0 19 0.0 0.0 0.0 0.08034496738289168 0.0 20 0.0 0.0 0.0 0.08403051634540964 0.0 21 0.0 0.0 0.0 0.08476762613791325 0.0 22 0.0 0.0 0.0 0.09287583385545277 0.0 23 0.0 0.0 0.0 0.09287583385545277 0.0 24 0.0 0.0 0.0 0.09950982198798511 0.0 25 0.0 0.0 0.0 0.10761802970552464 0.0 26 0.0 0.0 0.0 0.1334168724431504 0.0 27 0.0 0.0 0.0 0.23734935318615707 0.0 28 0.0 0.0 0.0 0.37445177459182544 0.0 29 0.0 0.0 0.0 0.5403014779051339 0.0 30 0.0 0.0 0.0 0.710573839973464 0.0 31 0.0 0.0 0.0 0.9051708251944127 0.0 32 0.0 0.0 0.0 1.1122986768879224 0.0 33 0.0 0.0 0.0 1.4027199351343382 0.0 34 0.0 0.0 0.0 1.8014963328787823 0.0 35 0.0 0.0 0.0 2.330004054103859 0.0 36 0.0 0.0 0.0 3.121659971252718 0.0 37 0.0 0.0 0.0 4.1956289389304535 0.0 38 0.0 0.0 0.0 5.260015479305642 0.0 39 0.0 0.0 0.0 6.176980061180113 0.0 40 0.0 0.00221132937751078 0.0 7.105001289942137 0.0 41 0.0 0.00221132937751078 0.0 8.080934655216895 0.0 42 0.0 0.00221132937751078 0.0 8.943353112446099 0.0 43 0.0 0.00221132937751078 0.0 9.931817344193417 0.0 44 0.0 0.00221132937751078 0.0 10.866472561087974 0.0 45 0.0 0.00221132937751078 0.0 11.839457487192718 0.0 46 0.0 0.00221132937751078 0.0 12.795488888069878 0.0 47 0.0 0.00221132937751078 0.0 13.663067113846607 0.0 48 0.0 0.00221132937751078 0.0 14.439243725352892 0.0 49 0.0 0.00221132937751078 0.0 15.215420336859175 0.0 50 0.0 0.00221132937751078 0.0 15.99601960712048 0.0 51 0.0 0.00221132937751078 0.0 16.739763387756607 0.0 52 0.0 0.00221132937751078 0.0 17.552058379095566 0.0 53 0.0 0.00221132937751078 0.0 18.22798805882136 0.0 54 0.0 0.00221132937751078 0.0 18.917922824604727 0.0 55 0.0 0.00221132937751078 0.0 19.639553311465743 0.0 56 0.0 0.00221132937751078 0.0 20.357498249364244 0.0 57 0.0 0.00221132937751078 0.0 20.989938451332325 0.0 58 0.0 0.00221132937751078 0.0 21.7027236206833 0.0 59 0.0 0.00221132937751078 0.0 22.348431798916447 0.0 60 0.0 0.00221132937751078 0.0 22.974975122544503 0.0 61 0.0 0.00221132937751078 0.0 23.642059484760257 0.0 62 0.0 0.00221132937751078 0.0 24.197840268307964 0.0 63 0.0 0.00221132937751078 0.0 24.786790992518334 0.0 64 0.0 0.00221132937751078 0.0 25.418494084693915 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATATA 15 0.0022206653 70.0 1 ACGGAAA 15 0.0022206653 70.0 31 ACGTTGA 20 0.006938894 52.499996 54 ATTACGG 20 0.006938894 52.499996 26 CGACAGG 20 0.006938894 52.499996 1 AACGGAA 20 0.006938894 52.499996 30 AATCGAG 20 0.006938894 52.499996 62 CTTATAA 50 1.7025576E-4 35.000004 1 TACGTAC 40 0.0024119448 34.999996 23 AATGATT 55 2.979655E-4 31.81818 25 CTGCTAT 45 0.004295207 31.111109 22 CCCTCAG 45 0.004295207 31.111109 19 CTGAGAC 45 0.004295207 31.111109 3 GCGCGAG 50 0.007188321 28.000002 41 TATGGCT 50 0.007188321 28.000002 4 GCAGTCC 50 0.007188321 28.000002 28 ACTACGT 50 0.007188321 28.000002 21 TGGCTTC 50 0.007188321 28.000002 22 GGTAGAA 50 0.007188321 28.000002 15 GTTGCCG 50 0.007188321 28.000002 18 >>END_MODULE